Molecular Diagnosis of Mycobacterium Intracellulare Infection in Patients with Kidney Transplantation_West China Medical Journal

Authors：

WANG Xiaohui ^1,2 , JIANG Yong ³ , ZHOU Taoyou ^1,2 ,  ZONG Zhiyong ^1,2

1. Center of Infectious Diseases; 2. Division of Infectious Diseases, State Key Laboratory of Biotherapy; 3. Department of Pathology; West China Hospital, Sichuan University, Chengdu, Sichuan 610041, P. R. China;

Corresponding author：

ZONG Zhiyong, Email: melodywxh@yahoo.com.cn

Keywords：

胞内分枝杆菌; 分子诊断; 肾移植; 皮肤软组织感染; hsp65基因

DOI：

10.7507/1002-0179.20130306

Video：

Export PDF Favorites Scan Get Citation

Abstract Full text Figures/Tables Video References Cited by

目的　探讨肾移植患者非结核分枝杆菌（NTM）病临床特点及分子诊断。方法　回顾性分析2011年4月1例皮肤软组织NTM感染的肾移植患者的临床特点，并以其病变组织DNA为模板，聚合酶链反应（PCR）扩增hsp65基因和rpoB基因序列，测序比对鉴定其NTM菌种。结合文献复习NTM病及分析分子生物学技术在移植患者NTM感染诊断中的作用。结果　该肾移植患者系皮肤软组织胞内分枝杆菌感染，临床特点与结核病极其相似，难以进行鉴别诊断。PCR扩增、测序的结果显示hsp65产物和rpoB产物序列与胞内分枝杆菌GeneBank中FJ643456.1及CP003324.1序列100%一致。结论　NTM病的临床表现与结核病相似，分子生物学方法鉴定菌种对移植患者胞内分枝杆菌病的诊断有帮助。

Citation： WANG Xiaohui,JIANG Yong,ZHOU Taoyou,ZONG Zhiyong,.. Molecular Diagnosis of Mycobacterium Intracellulare Infection in Patients with Kidney Transplantation. West China Medical Journal, 2013, 28(7): 984-987. doi: 10.7507/1002-0179.20130306 Copy

1.	全国第五次结核病流行病学抽样调查技术指导组. 2010年全国第五次结核病流行病学抽样调查报告[J]. 中国防痨杂志, 2012, 34(8): 485-508.
2.	Telenti A, Marchesi F, Balz M, et al. Rapid identification of mycobacteria to the species level by polymerase chain reaction and restriction enzyme analysis [J]. J Clin Microbiol, 1993, 31(2): 175-178.
3.	Kim BJ, Lee KH, Park BN, et al. Differentiation of mycobacterial species by PCR-restriction analysis of DNA (342 base pairs) of the RNA polymerase gene (rpoB) [J]. J Clin Microbiol, 2001, 39(6): 2102-2109.
4.	Dai J, Chen Y, Lauzardo M. Web-accessible database of hsp65 sequences from Mycobacterium reference strains [J]. J Clin Microbiol, 2011, 49(6): 2296-2303.
5.	Lai CC, Tan CK, Chou CH, et al. Increasing incidence of nontuberculous mycobacteria, Taiwan, 2000-2008 [J]. Emerg Infect Dis, 2010, 16(2): 294-296.
6.	Thomson RM. Changing epidemiology of pulmonary nontuberculous mycobacteria infections [J]. Emerg Infect Dis, 2010, 16(10): 1576-1583.
7.	Nicholson O, Feja K, LaRussa P, et al. Nontuberculous mycobacterial infections in pediatric hematopoietic stem cell transplant recipients: case report and review of the literature [J]. Pediatr Infect Dis J, 2006, 25(3): 263-267.
8.	Haas S, Scully B, Cohen D, et al. Mycobacterium avium complex infection in kidney transplant patients [J]. Transpl Infect Dis, 2005, 7(2): 75-79.
9.	Gupta A, Clauss H. Prosthetic joint infection with Mycobacterium avium complex in a solid organ transplant recipient [J]. Transpl Infect Dis, 2009, 11(6): 537-540.
10.	Caroti L, Zanazzi M, Rogasi P, et al. Subcutaneous nodules and infectious complications in renal allograft recipients [J]. Transplant Proc, 2010, 42(4): 1146-1147.
11.	Singh S, Yosypiv IV, Iorember FM. Disseminated mycobacterium avium complex infection in a pediatric renal transplant recipient [J]. Clin Pediatr (Phila), 2011, 51(9): 892-895.
12.	David NG, Robert CM, George M. Sanford guide to antimicrobial therapy [M]. 40 ed. Sperryville: Antimicrobial Therapy, 2011: 122-123.
13.	Kim BJ, Lee SH, Lyu MA, et al. Identification of mycobacterial species by comparative sequence analysis of the RNA polymerase gene (rpoB) [J]. J Clin Microbiol, 1999, 37(6): 1714-1720.
14.	McNabb A, Adie K, Rodrigues M, et al. Direct identification of mycobacteria in primary liquid detection media by partial sequencing of the 65-kilodalton heat shock protein gene [J]. J Clin Microbiol, 2006, 44(1): 60-66.
15.	Ong CS, Ngeow YF, Yap SF, et al. Evaluation of PCR-RFLP analysis targeting hsp65 and rpoB genes for the typing of mycobacterial isolates in Malaysia [J]. J Med Microbiol, 2010, 59(Pt 11): 1311-1316.

1. 　全国第五次结核病流行病学抽样调查技术指导组. 2010年全国第五次结核病流行病学抽样调查报告[J]. 中国防痨杂志, 2012, 34(8): 485-508.
2. 　Telenti A, Marchesi F, Balz M, et al. Rapid identification of mycobacteria to the species level by polymerase chain reaction and restriction enzyme analysis [J]. J Clin Microbiol, 1993, 31(2): 175-178.
3. 　Kim BJ, Lee KH, Park BN, et al. Differentiation of mycobacterial species by PCR-restriction analysis of DNA (342 base pairs) of the RNA polymerase gene (rpoB) [J]. J Clin Microbiol, 2001, 39(6): 2102-2109.
4. 　Dai J, Chen Y, Lauzardo M. Web-accessible database of hsp65 sequences from Mycobacterium reference strains [J]. J Clin Microbiol, 2011, 49(6): 2296-2303.
5. 　Lai CC, Tan CK, Chou CH, et al. Increasing incidence of nontuberculous mycobacteria, Taiwan, 2000-2008 [J]. Emerg Infect Dis, 2010, 16(2): 294-296.
6. 　Thomson RM. Changing epidemiology of pulmonary nontuberculous mycobacteria infections [J]. Emerg Infect Dis, 2010, 16(10): 1576-1583.
7. 　Nicholson O, Feja K, LaRussa P, et al. Nontuberculous mycobacterial infections in pediatric hematopoietic stem cell transplant recipients: case report and review of the literature [J]. Pediatr Infect Dis J, 2006, 25(3): 263-267.
8. 　Haas S, Scully B, Cohen D, et al. Mycobacterium avium complex infection in kidney transplant patients [J]. Transpl Infect Dis, 2005, 7(2): 75-79.
9. 　Gupta A, Clauss H. Prosthetic joint infection with Mycobacterium avium complex in a solid organ transplant recipient [J]. Transpl Infect Dis, 2009, 11(6): 537-540.
10. 　Caroti L, Zanazzi M, Rogasi P, et al. Subcutaneous nodules and infectious complications in renal allograft recipients [J]. Transplant Proc, 2010, 42(4): 1146-1147.
11. 　Singh S, Yosypiv IV, Iorember FM. Disseminated mycobacterium avium complex infection in a pediatric renal transplant recipient [J]. Clin Pediatr (Phila), 2011, 51(9): 892-895.
12. 　David NG, Robert CM, George M. Sanford guide to antimicrobial therapy [M]. 40 ed. Sperryville: Antimicrobial Therapy, 2011: 122-123.
13. 　Kim BJ, Lee SH, Lyu MA, et al. Identification of mycobacterial species by comparative sequence analysis of the RNA polymerase gene (rpoB) [J]. J Clin Microbiol, 1999, 37(6): 1714-1720.
14. 　McNabb A, Adie K, Rodrigues M, et al. Direct identification of mycobacteria in primary liquid detection media by partial sequencing of the 65-kilodalton heat shock protein gene [J]. J Clin Microbiol, 2006, 44(1): 60-66.
15. 　Ong CS, Ngeow YF, Yap SF, et al. Evaluation of PCR-RFLP analysis targeting hsp65 and rpoB genes for the typing of mycobacterial isolates in Malaysia [J]. J Med Microbiol, 2010, 59(Pt 11): 1311-1316.

Previous Article
Treatment of IgA Nephrophy with Low-dose Mycophenolate mofetil and Steroid
Next Article
易误诊的皮下脂膜炎样T细胞淋巴瘤一例

West China Medical Journal

Molecular Diagnosis of Mycobacterium Intracellulare Infection in Patients with Kidney Transplantation

Abstract Full text Figures/Tables Video References Cited by

Previous Article

Next Article

Format

Content