Neuroprotective Effect of Verapamil on Rabbit Spinal Cord Ischemia-Reperfusion Injury_West China Medical Journal

Authors：

TU Jianqi , LIU ZhiQiang.

Department of Anatomy, Jining Medical College, Jining, Shandong 272067, P. R. China;

Corresponding author：

Keywords：

缺血再灌注损伤; 兔; 维拉帕米

DOI：

10.7507/1002-0179.20130323

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目的　观察维拉帕米对兔脊髓缺血再灌注神经损伤的影响。方法　选用健康兔30只，随机分为3组，A组为假手术组（只穿线不结扎），B组为缺血再灌注组（腹主动脉缺血30 min后，再灌注48 h），C组为维拉帕米组（0.2 mg/kg维拉帕米静脉注射后，腹主动脉缺血30 min，再灌注48 h）。对3组动物行神经功能评价并通过病理组织学观察神经元细胞的变化，以此评价维拉帕米对兔脊髓缺血再灌注神经损伤的保护作用。结果　A组神经功能评价、神经元细胞的病理组织学观察均正常。B组神经功能出现明显障碍，神经元细胞形态异常。C组亦出现神经功能障碍，神经元细胞形态异常，但均较B组轻。结论　应用维拉帕米可减轻因脊髓缺血再灌注造成的神经损伤。

Citation： TU Jianqi,LIU ZhiQiang.. Neuroprotective Effect of Verapamil on Rabbit Spinal Cord Ischemia-Reperfusion Injury. West China Medical Journal, 2013, 28(7): 1045-1047. doi: 10.7507/1002-0179.20130323 Copy

1.	Mohan IK, Khan M, Wisel S, et al. Cardioprotection by HO-4038, a novel verapamil derivative, targeted against ischemia and reperfusion-mediated acute myocardial infarction[J]. Am J Physiol Heart Circ Physiol, 2009, 296(1): 140-151.
2.	Coetzee A, Conradie S. Calcium antagonist verapamil and reperfusion injury of the heart[J]. J Cardiothorac Vasc Anesth, 2007, 21(3): 337-343.
3.	Krebs P, Fan W, Chen YH, et al. Lethal mitochondrial cardiomyopathy in a hypomorphic Med30 mouse mutant is ameliorated by ketogenic diet[J]. Proc Natl Acad Sci USA, 2011, 108(49): 19678-19682.
4.	Turer AT, Hill JA. Pathogenesis of myocardial ischemia-reperfusion injury and rationale for therapy[J]. Am J Cardiol, 2010, 106(3): 360-368.
5.	魏育涛, 陈新忠, 董念国, 等. 脂联素对兔短期缺血再灌注心肌的保护作用[J]. 华中科技大学学报·医学版, 2010, 39(6): 750-753.
6.	Giorgi C, Romagnoli A, Pinton P, et al. Ca2+ signaling, mitochondria and cell death[J]. Curr Mol Med, 2008, 8(2): 119-130.
7.	Wang QD, Pernow J, Sjöquist PO, et al. Pharmacologi-cal possibilities for protection against myocardial reperfu-sion injury[J]. Cardiovasc Res, 2002, 55(1): 25-37.
8.	Murphy E, Steenbergen C. Ion transport and energetics during cell death and protection[J]. Physiology (Bethesda), 2008, 23(1): 115-123.
9.	Dong Z, Saikumar P, Weinberg JM, et al. Calcium in cell injury and death[J]. Annu Rev Pathol, 2006, 1(1): 405-434.
10.	González G, Zaldívar D, Carrillo E, et al. Pharmacological preconditioning by diazoxide downregulates cardiac L-type Ca2+ channels[J]. Br J Pharmacol, 2010, 161(5): 1172-1185.
11.	胡小琴. 心血管麻醉及体外循环[M]. 北京: 人民卫生出版社, 1997: 249- 249.
12.	徐成斌, 王思让, 李文彬. 心脏电生理学与药理学基础与临床[M]. 北京: 军事医学科学出版社, 1997: 254-254.
13.	孟春阳, 尹飞, 郭丽, 等. 银杏叶对大鼠脊髓缺血再灌注损伤保护作用的实验研究[J]. 中国实验诊断学, 2010, 14(6): 812-814.
14.	Yilmaz ER, Kertmen H, Dolgun H, et al. Effects of darbepoetin-α in spinal cord ischemia-reperfusion injury in the rabbit[J]. Acta Neurochir (Wien), 2012, 154(6): 1037-1043.
15.	Ucak A, Onan B, Güler A, et al. Rosuvastatin, a new generation 3-hydroxy-3-methylglutaryl coenzyme a reductase inhibitor, reduces ischemia/reperfusion-induced spinal cord tissue injury in rats[J]. Ann Vasc Surg, 2011, 25(5): 686-695.

1. 　Mohan IK, Khan M, Wisel S, et al. Cardioprotection by HO-4038, a novel verapamil derivative, targeted against ischemia and reperfusion-mediated acute myocardial infarction[J]. Am J Physiol Heart Circ Physiol, 2009, 296(1): 140-151.
2. 　Coetzee A, Conradie S. Calcium antagonist verapamil and reperfusion injury of the heart[J]. J Cardiothorac Vasc Anesth, 2007, 21(3): 337-343.
3. 　Krebs P, Fan W, Chen YH, et al. Lethal mitochondrial cardiomyopathy in a hypomorphic Med30 mouse mutant is ameliorated by ketogenic diet[J]. Proc Natl Acad Sci USA, 2011, 108(49): 19678-19682.
4. 　Turer AT, Hill JA. Pathogenesis of myocardial ischemia-reperfusion injury and rationale for therapy[J]. Am J Cardiol, 2010, 106(3): 360-368.
5. 　魏育涛, 陈新忠, 董念国, 等. 脂联素对兔短期缺血再灌注心肌的保护作用[J]. 华中科技大学学报·医学版, 2010, 39(6): 750-753.
6. 　Giorgi C, Romagnoli A, Pinton P, et al. Ca2+ signaling, mitochondria and cell death[J]. Curr Mol Med, 2008, 8(2): 119-130.
7. 　Wang QD, Pernow J, Sjöquist PO, et al. Pharmacologi-cal possibilities for protection against myocardial reperfu-sion injury[J]. Cardiovasc Res, 2002, 55(1): 25-37.
8. 　Murphy E, Steenbergen C. Ion transport and energetics during cell death and protection[J]. Physiology (Bethesda), 2008, 23(1): 115-123.
9. 　Dong Z, Saikumar P, Weinberg JM, et al. Calcium in cell injury and death[J]. Annu Rev Pathol, 2006, 1(1): 405-434.
10. 　González G, Zaldívar D, Carrillo E, et al. Pharmacological preconditioning by diazoxide downregulates cardiac L-type Ca2+ channels[J]. Br J Pharmacol, 2010, 161(5): 1172-1185.
11. 　胡小琴. 心血管麻醉及体外循环[M]. 北京: 人民卫生出版社, 1997: 249- 249.
12. 　徐成斌, 王思让, 李文彬. 心脏电生理学与药理学基础与临床[M]. 北京: 军事医学科学出版社, 1997: 254-254.
13. 　孟春阳, 尹飞, 郭丽, 等. 银杏叶对大鼠脊髓缺血再灌注损伤保护作用的实验研究[J]. 中国实验诊断学, 2010, 14(6): 812-814.
14. 　Yilmaz ER, Kertmen H, Dolgun H, et al. Effects of darbepoetin-α in spinal cord ischemia-reperfusion injury in the rabbit[J]. Acta Neurochir (Wien), 2012, 154(6): 1037-1043.
15. 　Ucak A, Onan B, Güler A, et al. Rosuvastatin, a new generation 3-hydroxy-3-methylglutaryl coenzyme a reductase inhibitor, reduces ischemia/reperfusion-induced spinal cord tissue injury in rats[J]. Ann Vasc Surg, 2011, 25(5): 686-695.

West China Medical Journal

Neuroprotective Effect of Verapamil on Rabbit Spinal Cord Ischemia-Reperfusion Injury

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