Serum and Synovial Fluid Levels of Interleukin-17A in Correlation with Disease Activity in Patients with Rheumatoid Arthritis_West China Medical Journal

Authors：

LIN Hui ¹ , CHEN Yongtao ¹ , ZHOU Yanli ² ,  LIU Yi. ¹

1. Department of Rheumatology, West China Hospital, Sichuan University, Chengdu, Sichuan 610041, P. R. China; 2. Department of Rheumatology, Luzhou Medical College, Luzhou, Sichuan 646000, P. R. China;

Corresponding author：

LIU Yi., Email: lhacd@163.com

Keywords：

类风湿关节炎; 血清; 关节液; 白细胞介素17A

DOI：

10.7507/1002-0179.20130482

Video：

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目的　检测类风湿关节炎（RA）患者血清和关节液白细胞介素17A（IL-17A）的变化，探讨其与临床炎症指标、疾病活动性的关系。方法　2011年6月－2012年6月采用酶联免疫吸附试验检测30例活动性RA患者和20例健康对照血清IL-17A水平，其中18例有膝关节积液RA患者同时检测配对血清和关节液IL-17A水平。结果　RA组患者血清IL-17A水平显著高于健康对照组[（40.651 ± 16.402）、（23.799 ± 10.693） pg/mL，P＜0.05]。RA患者关节液IL-17A水平明显高于其血清中水平[（63.555 ± 23.405）、（43.727 ± 17.212） pg/mL，P＜0.05]。RA患者血清IL-17A水平只与疾病活动性评分（DAS28）呈正相关（r=0.498，P=0.020），而RA患者关节液IL-17A水平与DAS28和血清C反应蛋白有相关性（r=0.515，P=0.029；r=0.498，P=0.035）。结论　RA患者血清和关节液IL-17A水平与疾病活动性显著相关，提示IL-17A可作为衡量疾病活动和关节损伤的标志之一。

Citation： LIN Hui,CHEN Yongtao,ZHOU Yanli,LIU Yi.. Serum and Synovial Fluid Levels of Interleukin-17A in Correlation with Disease Activity in Patients with Rheumatoid Arthritis. West China Medical Journal, 2013, 28(10): 1521-1524. doi: 10.7507/1002-0179.20130482 Copy

1.	Imboden JB. The immunopathogenesis of rheumatoid arthritis[J]. Annu Rev Pathol, 2009, 4: 417-434.
2.	Korn T, Bettelli E, Oukka M, et al. IL-17 and Th17 cells[J]. Annu Rev Immunol, 2009, 27: 485-517.
3.	Nakae S, Nambu A, Sudo K, et al. Suppression of immune induction of collagen-induced arthritis in IL-17-deficient mice[J]. J Immunol, 2003, 171(11): 6173-6177.
4.	Lubberts E, Koenders MI, Oppers-Walgreen B, et al. Treatment with a neutralizing anti-murine interleukin-17 antibody after the onset of collagen-induced arthritis reduces joint inflammation, cartilage destruction, and bone erosion[J]. Arthritis Rheum, 2004, 50(2): 650-659.
5.	Sarkar S, Fox DA. Targeting IL-17 and Th17 cells in rheumatoid arthritis[J]. Rheum Dis Clin North Am, 2010, 36(2): 345-366.
6.	Melis L, Vandooren B, Kruithof E, et al. Systemic levels of IL-23 are strongly associated with disease activity in rheumatoid arthritis but not spondyloarthritis[J]. Ann Rheum Dis, 2010, 69(3): 618-623.
7.	Hitchon CA, Alex P, Erdile LB, et al. A distinct multicytokine profile is associated with anti-cyclical citrullinated peptide antibodies in patients with early untreated inflammatory arthritis[J]. J Rheumatol, 2004, 31(12): 2336-2346.
8.	Mika K, Akito T, Hiroto M, et al. Interleukin-17 gene expression in patients with rheumatoid arthritis[J]. Mod Rheumatol, 2008, 18(1): 15-22.
9.	Yamada H, Nakashima Y, Okazaki K, et al. Th1 but not Th17 cells predominate in the joints of patients with rheumatoid arthritis[J]. Ann Rheum Dis, 2008, 67(9): 1299-1304.
10.	Cho ML, Yoon CH, Hwang SY, et al. Effector function of typeⅡ collagen-stimulated T cells from rheumatoid arthritis patients: cross-talk between T cells and synovial fibroblasts[J]. Arthritis Rheum, 2004, 50(3): 776-784.
11.	Zhu JF, Yamane H, Paul WE. Differentiation of effector CD4 T cell populations[J]. Annu Rev Immunol, 2010, 28: 445-489.
12.	卢月, 曹雅晶, 钱瑞琴. IL-17在类风湿关节炎发病机制中的作用[J]. 中国免疫学杂志, 2011, 27(9): 855-859.
13.	Hwang SY, Kim JY, Kim KW, et al. IL-17 induces production of IL-6 and IL-8 in rheumatoid arthritis synovial fibroblasts via NF-kappaB- and PI3-kinase/Akt-dependent pathways[J]. Arthritis Res Ther, 2004, 6(2): 120-128.
14.	Moran EM, Connolly M, Gao W, et al. Interleukin-17a induction of angiogenesis, cell migration, and cytoskeletal rearrangement[J]. Arthritis Rheum, 2011, 63(11): 3263-3273.
15.	Shahrara S, Pickens SR, Mandelin AM, et al. IL-17-mediated monocyte migration occurs partially through CC chemokine ligand 2/monocyte chemoattractant protein-1 induction[J]. J Immunol, 2010, 184(8): 4479-4487.
16.	Van HJ, Asmawidjaja PS, Davelaar N, et al. Th17 cells, but not Th1 cells, from patients with early rheumatoid arthritis are potent inducers of matrix metalloproteinases and proinflammatory cytokines upon synovial fibroblast interaction,including autocrine interleukin-17A production[J]. Arthritis Rheum, 2011, 63(1): 73-83.
17.	Pöllinger B, Junt T, Metzler B, et al. Th17 cells, not IL-17+γδ T cells, drive arthritic bone destruction in mice and humans[J]. J Immunol, 2011, 186(4): 2602-2612.
18.	Braun T, Zwerina J. Positive regulators of osteoclastogenesis and bone resorption in rheumatoid arthritis[J]. Arthritis Res Ther, 2011, 13(4): 235.
19.	Mitsdoerffer M, Lee Y, Jäger A, et al. Proinflammatory T helper type 17 cells are effective B-cell helpers[J]. Proc Natl Acad Sci USA, 2010, 107(32): 14292-14297.
20.	Eklund CM. Proinflammatory cytokines in CRP baseline regulation[J]. Adv Clin Chem, 2009, 48: 111-136.

1. 　Imboden JB. The immunopathogenesis of rheumatoid arthritis[J]. Annu Rev Pathol, 2009, 4: 417-434.
2. 　Korn T, Bettelli E, Oukka M, et al. IL-17 and Th17 cells[J]. Annu Rev Immunol, 2009, 27: 485-517.
3. 　Nakae S, Nambu A, Sudo K, et al. Suppression of immune induction of collagen-induced arthritis in IL-17-deficient mice[J]. J Immunol, 2003, 171(11): 6173-6177.
4. 　Lubberts E, Koenders MI, Oppers-Walgreen B, et al. Treatment with a neutralizing anti-murine interleukin-17 antibody after the onset of collagen-induced arthritis reduces joint inflammation, cartilage destruction, and bone erosion[J]. Arthritis Rheum, 2004, 50(2): 650-659.
5. 　Sarkar S, Fox DA. Targeting IL-17 and Th17 cells in rheumatoid arthritis[J]. Rheum Dis Clin North Am, 2010, 36(2): 345-366.
6. 　Melis L, Vandooren B, Kruithof E, et al. Systemic levels of IL-23 are strongly associated with disease activity in rheumatoid arthritis but not spondyloarthritis[J]. Ann Rheum Dis, 2010, 69(3): 618-623.
7. 　Hitchon CA, Alex P, Erdile LB, et al. A distinct multicytokine profile is associated with anti-cyclical citrullinated peptide antibodies in patients with early untreated inflammatory arthritis[J]. J Rheumatol, 2004, 31(12): 2336-2346.
8. 　Mika K, Akito T, Hiroto M, et al. Interleukin-17 gene expression in patients with rheumatoid arthritis[J]. Mod Rheumatol, 2008, 18(1): 15-22.
9. 　Yamada H, Nakashima Y, Okazaki K, et al. Th1 but not Th17 cells predominate in the joints of patients with rheumatoid arthritis[J]. Ann Rheum Dis, 2008, 67(9): 1299-1304.
10. 　Cho ML, Yoon CH, Hwang SY, et al. Effector function of typeⅡ collagen-stimulated T cells from rheumatoid arthritis patients: cross-talk between T cells and synovial fibroblasts[J]. Arthritis Rheum, 2004, 50(3): 776-784.
11. 　Zhu JF, Yamane H, Paul WE. Differentiation of effector CD4 T cell populations[J]. Annu Rev Immunol, 2010, 28: 445-489.
12. 　卢月, 曹雅晶, 钱瑞琴. IL-17在类风湿关节炎发病机制中的作用[J]. 中国免疫学杂志, 2011, 27(9): 855-859.
13. 　Hwang SY, Kim JY, Kim KW, et al. IL-17 induces production of IL-6 and IL-8 in rheumatoid arthritis synovial fibroblasts via NF-kappaB- and PI3-kinase/Akt-dependent pathways[J]. Arthritis Res Ther, 2004, 6(2): 120-128.
14. 　Moran EM, Connolly M, Gao W, et al. Interleukin-17a induction of angiogenesis, cell migration, and cytoskeletal rearrangement[J]. Arthritis Rheum, 2011, 63(11): 3263-3273.
15. 　Shahrara S, Pickens SR, Mandelin AM, et al. IL-17-mediated monocyte migration occurs partially through CC chemokine ligand 2/monocyte chemoattractant protein-1 induction[J]. J Immunol, 2010, 184(8): 4479-4487.
16. 　Van HJ, Asmawidjaja PS, Davelaar N, et al. Th17 cells, but not Th1 cells, from patients with early rheumatoid arthritis are potent inducers of matrix metalloproteinases and proinflammatory cytokines upon synovial fibroblast interaction,including autocrine interleukin-17A production[J]. Arthritis Rheum, 2011, 63(1): 73-83.
17. 　Pöllinger B, Junt T, Metzler B, et al. Th17 cells, not IL-17+γδ T cells, drive arthritic bone destruction in mice and humans[J]. J Immunol, 2011, 186(4): 2602-2612.
18. 　Braun T, Zwerina J. Positive regulators of osteoclastogenesis and bone resorption in rheumatoid arthritis[J]. Arthritis Res Ther, 2011, 13(4): 235.
19. 　Mitsdoerffer M, Lee Y, Jäger A, et al. Proinflammatory T helper type 17 cells are effective B-cell helpers[J]. Proc Natl Acad Sci USA, 2010, 107(32): 14292-14297.
20. 　Eklund CM. Proinflammatory cytokines in CRP baseline regulation[J]. Adv Clin Chem, 2009, 48: 111-136.

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Serum and Synovial Fluid Levels of Interleukin-17A in Correlation with Disease Activity in Patients with Rheumatoid Arthritis

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