The relationship between the ratio of expression of heat shock protein 70/ glial fibrillary acidic protein and post-injury interval after concussion of brain in rats_West China Medical Journal

Authors：

DAI Hao ¹ , CHEN Bo ² , JIN Wei ¹ ,  LIU Min ¹

1. Department of Forensic Pathology, West China School of Basic and Forensic Medicine, Sichuan University, Chengdu, Sichuan 610041, P. R. China;
2. Criminal Science and Technology Office, Jianyang Public Security Bureau, Jianyang, Sichuan 641499, P. R. China;

Corresponding author：

LIU Min, Email: min8liu@hotmail.com

Keywords：

Concussion of Brain; Post-injury interval; Heat shock protein 70; Glial fibrillary acidic protein; The ratio of percentage of positive cells; Rat

DOI：

10.7507/1002-0179.201611100

Video：

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Objectives To detect expressions of heat shock protein 70 (HSP70) and glial fibrillary acidic protein (GFAP) , and to estimate the post-injury interval after concussion of brain via the ratios of percentage of HSP70/GFAP-positive cells. Methods We established a brain concussion model of rat. Tissue levels of HSP70 and GFAP were determined by immunohistochemical staining at different time points after injury. Finally, the relationship between the ratio of percentage of HSP70/GFAP-positive cells and the post-injury interval was measured. Results The ratio of percentage of positive cells (increased from 7.15 to 11.73) and the percentage of HSP70-positive cells (P<0.05, compared with control group) increased, and the percentage of GFAP-positive cells did not change remarkably (P<0.05, compared with control group); the post-injury interval was between 0.5 hour and 3 hours. High ratio (>6.66) and high percentage of HSP70 and GFAP-positive cells (P<0.05, compared with control group) indicated the post-injury interval was between 3 and 12 hours. A low ratio (<6.66) and high percentage of HSP70 and GFAP-positive cells (P<0.05, compared with control group) suggested that the post-injury interval was later than 12 hours. Conclusion By analyzing the variation rule of the ratio of percentage positive cells after brain concussion, the post-injury interval after concussion of brain could be estimated.

Citation： DAI Hao, CHEN Bo, JIN Wei, LIU Min. The relationship between the ratio of expression of heat shock protein 70/ glial fibrillary acidic protein and post-injury interval after concussion of brain in rats. West China Medical Journal, 2017, 32(12): 1890-1894. doi: 10.7507/1002-0179.201611100 Copy

1.	Olczak M, Niderla-Bielińska J, Kwiatkowska M, et al. Tau protein (MAPT) as a possible biochemical marker of traumatic brain injury in postmortem examination. Forensic Sci Int, 2017, 280: 1-7.
2.	Defazio MV, Rammo RA, Robles JR, et al. The potential utility of blood-derived biochemical markers as indicators of early clinical trends following severe traumatic brain injury. World Neurosurg, 2014, 81(1): 151-158.
3.	胡玉莲, 许光亚, 刘敏. 大鼠脑挫伤后脑 C-FOS、AQP-4 表达与损伤时间的关系. 中国法医学杂志, 2015, 30(1): 24-28.
4.	陈锐, 严治, 胡玲, 等. 大鼠脑震荡模型建立法的改良和评价. 广东医学院学报, 2010, 28(4): 362-363.
5.	周亦武, 张益鹄, 邓伟年, 等. 87 例大脑及脑干挫伤 HSP70 变化的研究. 中国法医学杂志, 2005, 20(4): 209-212.
6.	Zhang J, Tao DQ, Zhao H, et al. Expression of Hsp70 and caspase-3 in rabbits after severe traumatic brain injury. Chin J Traumatol, 2012, 15(6): 338-341.
7.	Brenner M. Role of GFAP in CNS injuries. Neurosci Lett, 2014, 565(17): 7-13.
8.	李玉红, 闫平, 孟晓萍, 等. 实验性大鼠颅脑损伤后 GFAP 的表达研究. 伤残医学杂志, 2004, 12(2): 1-3.
9.	Stewart A, Tekes A, Huisman TA, et al. Glial fibrillary acidic protein as a biomarker for periventricular white matter injury. Am J Obstet Gynecol, 2013, 209(1): 27.e1-27.e7.
10.	Stetler RA, Gan Y, Zhang W, et al. Heat shock proteins: cellular and molecular mechanisms in the central nervous system. Prog Neurobiol, 2010, 92(2): 184-211.
11.	Bunch H. RNA polymeraseⅡpausing and transcriptional regulation of the HSP70 expression. Eur J Cell Biol, 2017, 96(8): 739-745.
12.	曾丽容, 郑定容. 急性颅脑损伤脑脊液 IL-19、IL-18 与热休克蛋白-70 变化及意义. 中华临床医师杂志: 电子版, 2015, 9(4): 684-686.
13.	金伟, 夏勇军, 李雪榕, 等. 大鼠脑震荡伤后脑外主要器官热休克蛋白 70、C-fos 表达比值与损伤时间变化的关系研究. 华西医学, 2017, 32(10): 1539-1543.
14.	陈锐, 余彬华, 胡玲, 等. 大鼠脑震荡伤后脑组织 HSP70、bFGF 及TGF-β₁ 表达变化. 法医学杂志, 2009, 25(4): 249-253.
15.	郝芳芳, 贺婕, 孙志华, 等. 蛛网膜下腔出血患者 GFAP、S100B 的表达与预后相关性研究. 检验医学与临床, 2017, 14(20): 3061-3063.
16.	Liu MD, Luo P, Wang ZJ, et al. Changes of serum Tau, GFAP, TNF-α and malonaldehyde after blast-related traumatic brain injury. Chin J Traumatol, 2014, 17(6): 317-322.
17.	Singh K, Trivedi R, Devi MM, et al. Longitudinal changes in the DTI measures, anti-GFAP expression and levels of serum inflammatory cytokines following mild traumatic brain injury. Exp Neurol, 2016, 275(Pt 3): 427-435.

1. Olczak M, Niderla-Bielińska J, Kwiatkowska M, et al. Tau protein (MAPT) as a possible biochemical marker of traumatic brain injury in postmortem examination. Forensic Sci Int, 2017, 280: 1-7.
2. Defazio MV, Rammo RA, Robles JR, et al. The potential utility of blood-derived biochemical markers as indicators of early clinical trends following severe traumatic brain injury. World Neurosurg, 2014, 81(1): 151-158.
3. 胡玉莲, 许光亚, 刘敏. 大鼠脑挫伤后脑 C-FOS、AQP-4 表达与损伤时间的关系. 中国法医学杂志, 2015, 30(1): 24-28.
4. 陈锐, 严治, 胡玲, 等. 大鼠脑震荡模型建立法的改良和评价. 广东医学院学报, 2010, 28(4): 362-363.
5. 周亦武, 张益鹄, 邓伟年, 等. 87 例大脑及脑干挫伤 HSP70 变化的研究. 中国法医学杂志, 2005, 20(4): 209-212.
6. Zhang J, Tao DQ, Zhao H, et al. Expression of Hsp70 and caspase-3 in rabbits after severe traumatic brain injury. Chin J Traumatol, 2012, 15(6): 338-341.
7. Brenner M. Role of GFAP in CNS injuries. Neurosci Lett, 2014, 565(17): 7-13.
8. 李玉红, 闫平, 孟晓萍, 等. 实验性大鼠颅脑损伤后 GFAP 的表达研究. 伤残医学杂志, 2004, 12(2): 1-3.
9. Stewart A, Tekes A, Huisman TA, et al. Glial fibrillary acidic protein as a biomarker for periventricular white matter injury. Am J Obstet Gynecol, 2013, 209(1): 27.e1-27.e7.
10. Stetler RA, Gan Y, Zhang W, et al. Heat shock proteins: cellular and molecular mechanisms in the central nervous system. Prog Neurobiol, 2010, 92(2): 184-211.
11. Bunch H. RNA polymeraseⅡpausing and transcriptional regulation of the HSP70 expression. Eur J Cell Biol, 2017, 96(8): 739-745.
12. 曾丽容, 郑定容. 急性颅脑损伤脑脊液 IL-19、IL-18 与热休克蛋白-70 变化及意义. 中华临床医师杂志: 电子版, 2015, 9(4): 684-686.
13. 金伟, 夏勇军, 李雪榕, 等. 大鼠脑震荡伤后脑外主要器官热休克蛋白 70、C-fos 表达比值与损伤时间变化的关系研究. 华西医学, 2017, 32(10): 1539-1543.
14. 陈锐, 余彬华, 胡玲, 等. 大鼠脑震荡伤后脑组织 HSP70、bFGF 及TGF-β₁ 表达变化. 法医学杂志, 2009, 25(4): 249-253.
15. 郝芳芳, 贺婕, 孙志华, 等. 蛛网膜下腔出血患者 GFAP、S100B 的表达与预后相关性研究. 检验医学与临床, 2017, 14(20): 3061-3063.
16. Liu MD, Luo P, Wang ZJ, et al. Changes of serum Tau, GFAP, TNF-α and malonaldehyde after blast-related traumatic brain injury. Chin J Traumatol, 2014, 17(6): 317-322.
17. Singh K, Trivedi R, Devi MM, et al. Longitudinal changes in the DTI measures, anti-GFAP expression and levels of serum inflammatory cytokines following mild traumatic brain injury. Exp Neurol, 2016, 275(Pt 3): 427-435.

West China Medical Journal

The relationship between the ratio of expression of heat shock protein 70/ glial fibrillary acidic protein and post-injury interval after concussion of brain in rats

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