SHI Shaorui 1 , NIE Bin 1 , GUO Yu 1 , LUO Li 1 , ZHANG Linlin 1 , LI Wenquan 2 , Xian Gongbing 2 , WU Jialiang 2 , LIN Chunxing 3 , CHEN Xinzu 4,5
  • 1. Department of Clinical Laboratory, the Second People’s Hospital of Yibin · West China Yibin Hospital, Sichuan University, Yibin, Sichuan 644000, P. R. China;
  • 2. No.2 Department of Infecious Diseases, the Second People’s Hospital of Yibin · West China Yibin Hospital, Sichuan University, Yibin, Sichuan 644000, P. R. China;
  • 3. Department of Research and Education, the Second People’s Hospital of Yibin · West China Yibin Hospital, Sichuan University, Yibin, Sichuan 644000, P. R. China;
  • 4. Department of Gastrointestinal and Hernia Surgery, the Second People’s Hospital of Yibin · West China Yibin Hospital, Sichuan University, Yibin, Sichuan 644000, P. R. China;
  • 5. Department of Gastrointestinal Surgery, West China Hospital, Sichuan University, Chengdu, Sichuan 610041, P. R. China;
NIE Bin, Email: nb1995703@126.com; LI Wenquan, Email: 903394570@qq.com; CHEN Xinzu, Email: chenxinzu@scu.edu.cn
Real-time reverse transcriptase-polymerase chain reaction; 2019 novel coronavirus; Novel coronavirus pneumonia
10.7507/1002-0179.202002063
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Objective To detect the 2019 novel coronavirus (2019-nCoV) in various biological specimens of novel coronavirus pneumonia (NCP), and preliminarily observe the status of 2019-nCoV in different systems of the body and its clinical significance.Methods The study design was a small-scale cross-sectional observational study. All the confirmed NCP cases being treated in the Second People’s Hospital of Yibin · West China Yibin Hospital, Sichuan University on February 2nd, 2020 were enrolled in this study. Two sets of primers were designed for 2019-nCoV-1ab and N regions using real-time reverse transcriptase-polymerase chain reaction (RT-PCR) assay. The 2019-nCoV in upper respiratory specimens, blood, feces and urine specimens of the NCP cases were detected on the single day.Results A total of 7 imported NCP cases (mild type) were included. The 7 patients were confirmed by the positive results of 2019-nCoV nucleic acid tests of upper or lower respiratory specimens between the 3rd day and the 7th day after fever onset, while 2 patients were found positive on the 3rd day after onset. The 2019-nCoV nucleic acid tests of the 7 patients were detected again on a single day between the 7th day and the 15th day after onset, and the results showed: the upper respiratory specimens of 5 patients were found negative (1 case was on the 7th day after onset); 2019-nCoV was not detected in the blood, feces or urine specimens of the 7 patients.Conclusions For mild type NCP patients, real-time RT-PCR test could detect 2019-nCoV between the 3rd day and the 7th day after onset, while 2019-nCoV might become negative since the 7th day after onset. 2019-nCoV was not detected in the blood, feces or urine of mild type NCP patients on the single day between the 7th day and the 15th day after onset. This study was only a preliminary observational study, which needed high-qualified studies to obtain more definitive conclusions.

Citation： SHI Shaorui, NIE Bin, GUO Yu, LUO Li, ZHANG Linlin, LI Wenquan, Xian Gongbing, WU Jialiang, LIN Chunxing, CHEN Xinzu. Detection of 2019 novel coronavirus in various biological specimens of novel coronavirus pneumonia. West China Medical Journal, 2020, 35(2): 132-136. doi: 10.7507/1002-0179.202002063 Copy

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