RELATIONSHIP BETWEEN Fas EXPRESSION AND RECOVERY OF NEUROLOGICAL FUNCTION AFTER SURGICAL DECOMPRESSION IN SPINAL CORD INJURY RAT MODEL_Chinese Journal of Reparative and Reconstructive Surgery

Authors：

LI Quanhui ^1,2 , GU Wenhao ² , XIE Jiabing ² , XU Zhujun ²

1Second Department of Orthopaedics, the People’s Hospital of Yingshang County, Yingshang Anhui, 236200, P.R.China;;
2Department of Trauma Orthopaedics, Yejishan Hospital Affiliated to Wannan Medical College. Corresponding author: XU Zhujun, E-mail: WH4305@vip.sina.com;

Corresponding author：

Keywords：

Spinal cord injury; Nerve decompression; Fas; Apoptosis; Rat

DOI：

10.7507/1002-1892.20130156

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Objective To investigate the relationship between the expression of apoptosis-related gene Fas and recovery of neurological function after surgical decompression at different time points in acute spinal cord injury (SCI) rat model by cerclage. Methods A total of 100 13-week-old male Sprague Dawley rats (weighing, 255-376 g) were randomly divided into 4 groups (n=25). The rats only received laminectomy in group A as control; the rats were made the acute SCI models by cerclage in groups B, C, and D. The spinal cord decompression was performed in group B at 8 hours and in group C at 72 hours, no spinal cord decompression in group D. At 1, 3, 7, 14, and 21 days, Basso-Beattie-Bresnahan (BBB) score and inclined plane test were used to evaluate the recovery of neurological function; the neuronal apoptosis level of spinal cord was examined by TUNEL staining; HE staining and immunohistochemical staining were applied to analyze the expressions of Fas. Results The BBB score and inclined plane test score in group A were significantly better than those in groups B, C, and D at different time points (P lt; 0.05); group B was significantly better than groups C and D, and group C than group D at 3, 7, 14, and 21 days (P lt; 0.05). In group A, no bleeding, edema, or necrosis was found. The edema, hemorrhage, and neuron death were observed in spinal cord tissue of groups B, C, and D at 1 day after operation, especially in group D. The degree of cell degeneration in group B was lighter than that in groups C and D at 3 and 7 days after operation; few glial cells and fibroblast proliferation were found at damaged zone in group B at 14 and 21 days, but necrosis and cystic cavity in groups C and D. Fas and TUNEL expression was little in group A at different time points. Fas and TUNEL were expressed in groups B, C, and D; the expressions of Fas and TUNEL reached the maximum at 3 days, and then gradually decreased at 7 and 21 days. The number of positive cells was highest in group D, and the number of positive cells in group B was significantly less than that in groups C and D (P lt; 0.05). Conclusion Early decompression of SCI is beneficial to recovering the neurological function. The Fas signal pathway may play an important role in the apoptosis of neuron and glial cells after SCI.

Citation： LI Quanhui,GU Wenhao,XIE Jiabing,XU Zhujun. RELATIONSHIP BETWEEN Fas EXPRESSION AND RECOVERY OF NEUROLOGICAL FUNCTION AFTER SURGICAL DECOMPRESSION IN SPINAL CORD INJURY RAT MODEL. Chinese Journal of Reparative and Reconstructive Surgery, 2013, 27(6): 708-714. doi: 10.7507/1002-1892.20130156 Copy

1.	Baptiste DC, Fehlinqus MG. Pharmacological approaches to repair the injured spinal cord. J Neurotrauma, 2006, 23(3-4): 318-334.
2.	Esposito E, Mazzon E, Paterniti I, et al. Olprinone attenuates the acute inflammatory response and apoptosis after spinal cord strauma in mice. PloS One, 2010, 5(9): e12170.
3.	徐祝军, 王兵, 杨民, 等. 环扎法致大鼠脊髓损伤后早期不同时段减压疗效的评价. 中华实验外科杂志, 2011, 28(7): 1191-1192.
4.	Basso DM, Beattie MS, Bresnahan JC. A sensitive and reliable locomotor rating scale for open field testing in rats. J Neurotrauma, 1995, 12(1): 1-21.
5.	Cheng H, Cao Y, Olson L. Spinal cord repair in adult paraplegic rats: partial restoration of hind limb function. Science, 1996, 273(5274): 510-513.
6.	李亚涛, 谢怀春, 罗伟, 等. 前路减压与植骨内固定治疗胸腰椎骨折. 中国修复重建外科杂志, 2006, 20(6): 683-684.
7.	任忠明, 金才益, 裴斐, 等. 前路减压内固定修复严重胸腰椎爆裂骨折. 中国修复重建外科杂志, 2006, 20(4): 397-399.
8.	Marco RA, Kushwaha VP. Thoracolumbar burst fractures treated with posterior decompression and pedicle screw instrumentation supplemented with balloon-assisted vertebroplasty and calcium phosphate reconstruction. J Bone Joint Surg (Am), 2009, 9(1): 20-28.
9.	Fehlings MG, Perrin RG. The timing of surgical intervention in thet reatment of spinal cord injury: a systematic review of recent clinicalevidence. Spine (Phila Pa 1976), 2006, 31(11 Suppl): S28-36.
10.	Cengiz SL, Kalkan E, Bayir A, et al. Timing of thoracolomberspine stabilization in trauma patients, impact on neurologicaloutcome and clinical course: a real prospective (rct) randomizedcontrolled study. Arch Orthop Trauma Surg, 2008, 128(9): 959-966.
11.	林斌, 王宁, 沈忠美. 手术减压时间对兔颈脊髓急性损伤并压迫模型的疗效影响. 中国骨与关节损伤杂志, 2010, 25(5): 411-413.
12.	Gris P, Mushy S, Jacob JE, et al. Diferential gene expression profiles in embryonic, adult-injured and adult-uninjured rat spinal cords. Mol Cell Neurosci, 2003, 24(3): 555-567.
13.	Ray SK, Matzelle DD, Sribnick EA, et al. Calpain inhibitor prevented apoptosis and maintained transcription of proteolipid protein and myelin basis protein genes in rat spinal cord injury. J Chem Neuroanat, 2003, 26(2): 119-124．.
14.	Matthess Y, Raab M, Sanhaji M, et al. Cdk/cyclin B1 controls Fas-mediated apoptosis by regulating caspase-8 activity. Mol Cell Biol, 2010, 30(24): 5726-5740.
15.	Strasser A, Tos PJ, Naguta S. The many roles of Fas receptor signal in the immune system. Immunity, 2009, 30(2): 180-192.
16.	Shuman SL, Bresnahan JC, Beattie MS. Apoptosisof microglia and oligodendrocytes after spinal cord contusion in rats. J Neuroscience Res, 1997, 50(5): 798-808.
17.	李京, 孙善全, 朱淑娟, 等. 大鼠椎管减压与细胞凋亡的相关性研究. 重庆医科大学学报, 2010 , 35(1): 1-4.
18.	杨永利, 刘宏志. 大鼠急性脊髓损伤后神经细胞凋亡及Fas和Cathepsin D 的表达. 解剖学研究, 2012 , 34(4): 272-274, 289.
19.	杨冬娣, 刘金元. 青篙琥酯对肝纤维化小鼠肝星状细胞凋亡及其相关蛋白的影响. 长春中医药大学学报, 2009, 23(5): 323-324.
20.	邱炯, 李法琦. 半胱氨酸天冬氨酸蛋白酶与心血管疾病. 中国临床康复, 2005, 9(43): 145-147.
21.	Yu WR, Liu T, Kiehl TR, et al. Human neuropathological and animal model evidence supporting a role for Fas-mediated apoptosis and inflammation in cervical spondylotic myelopathy. Brain, 2011, 134(Pt 5): 1277-1292.
22.	Austin JW, Fehlings MG. Molecular mechanisms of Fas-mediated cell death in oligodendrocytes. J Neurotrauma, 2008, 25(5): 411-426.
23.	林院, 吴世强, 徐杰, 等. β-干扰素对SD大鼠急性脊髓损伤后Bax、Fas、Bcl-2基因表达的影响. 福建医药杂志, 2011, 33(5): 70-72.

1. Baptiste DC, Fehlinqus MG. Pharmacological approaches to repair the injured spinal cord. J Neurotrauma, 2006, 23(3-4): 318-334.
2. Esposito E, Mazzon E, Paterniti I, et al. Olprinone attenuates the acute inflammatory response and apoptosis after spinal cord strauma in mice. PloS One, 2010, 5(9): e12170.
3. 徐祝军, 王兵, 杨民, 等. 环扎法致大鼠脊髓损伤后早期不同时段减压疗效的评价. 中华实验外科杂志, 2011, 28(7): 1191-1192.
4. Basso DM, Beattie MS, Bresnahan JC. A sensitive and reliable locomotor rating scale for open field testing in rats. J Neurotrauma, 1995, 12(1): 1-21.
5. Cheng H, Cao Y, Olson L. Spinal cord repair in adult paraplegic rats: partial restoration of hind limb function. Science, 1996, 273(5274): 510-513.
6. 李亚涛, 谢怀春, 罗伟, 等. 前路减压与植骨内固定治疗胸腰椎骨折. 中国修复重建外科杂志, 2006, 20(6): 683-684.
7. 任忠明, 金才益, 裴斐, 等. 前路减压内固定修复严重胸腰椎爆裂骨折. 中国修复重建外科杂志, 2006, 20(4): 397-399.
8. Marco RA, Kushwaha VP. Thoracolumbar burst fractures treated with posterior decompression and pedicle screw instrumentation supplemented with balloon-assisted vertebroplasty and calcium phosphate reconstruction. J Bone Joint Surg (Am), 2009, 9(1): 20-28.
9. Fehlings MG, Perrin RG. The timing of surgical intervention in thet reatment of spinal cord injury: a systematic review of recent clinicalevidence. Spine (Phila Pa 1976), 2006, 31(11 Suppl): S28-36.
10. Cengiz SL, Kalkan E, Bayir A, et al. Timing of thoracolomberspine stabilization in trauma patients, impact on neurologicaloutcome and clinical course: a real prospective (rct) randomizedcontrolled study. Arch Orthop Trauma Surg, 2008, 128(9): 959-966.
11. 林斌, 王宁, 沈忠美. 手术减压时间对兔颈脊髓急性损伤并压迫模型的疗效影响. 中国骨与关节损伤杂志, 2010, 25(5): 411-413.
12. Gris P, Mushy S, Jacob JE, et al. Diferential gene expression profiles in embryonic, adult-injured and adult-uninjured rat spinal cords. Mol Cell Neurosci, 2003, 24(3): 555-567.
13. Ray SK, Matzelle DD, Sribnick EA, et al. Calpain inhibitor prevented apoptosis and maintained transcription of proteolipid protein and myelin basis protein genes in rat spinal cord injury. J Chem Neuroanat, 2003, 26(2): 119-124．.
14. Matthess Y, Raab M, Sanhaji M, et al. Cdk/cyclin B1 controls Fas-mediated apoptosis by regulating caspase-8 activity. Mol Cell Biol, 2010, 30(24): 5726-5740.
15. Strasser A, Tos PJ, Naguta S. The many roles of Fas receptor signal in the immune system. Immunity, 2009, 30(2): 180-192.
16. Shuman SL, Bresnahan JC, Beattie MS. Apoptosisof microglia and oligodendrocytes after spinal cord contusion in rats. J Neuroscience Res, 1997, 50(5): 798-808.
17. 李京, 孙善全, 朱淑娟, 等. 大鼠椎管减压与细胞凋亡的相关性研究. 重庆医科大学学报, 2010 , 35(1): 1-4.
18. 杨永利, 刘宏志. 大鼠急性脊髓损伤后神经细胞凋亡及Fas和Cathepsin D 的表达. 解剖学研究, 2012 , 34(4): 272-274, 289.
19. 杨冬娣, 刘金元. 青篙琥酯对肝纤维化小鼠肝星状细胞凋亡及其相关蛋白的影响. 长春中医药大学学报, 2009, 23(5): 323-324.
20. 邱炯, 李法琦. 半胱氨酸天冬氨酸蛋白酶与心血管疾病. 中国临床康复, 2005, 9(43): 145-147.
21. Yu WR, Liu T, Kiehl TR, et al. Human neuropathological and animal model evidence supporting a role for Fas-mediated apoptosis and inflammation in cervical spondylotic myelopathy. Brain, 2011, 134(Pt 5): 1277-1292.
22. Austin JW, Fehlings MG. Molecular mechanisms of Fas-mediated cell death in oligodendrocytes. J Neurotrauma, 2008, 25(5): 411-426.
23. 林院, 吴世强, 徐杰, 等. β-干扰素对SD大鼠急性脊髓损伤后Bax、Fas、Bcl-2基因表达的影响. 福建医药杂志, 2011, 33(5): 70-72.

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Chinese Journal of Reparative and Reconstructive Surgery

RELATIONSHIP BETWEEN Fas EXPRESSION AND RECOVERY OF NEUROLOGICAL FUNCTION AFTER SURGICAL DECOMPRESSION IN SPINAL CORD INJURY RAT MODEL

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