Experimental study on promotion of peripheral nerve regeneration by selenium-methylselenocysteine_Chinese Journal of Reparative and Reconstructive Surgery

Authors：

SONG Jianguo ,  LIN Haodong

Department of Trauma Center, Shanghai General Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, 201620, P. R. China;

Corresponding author：

LIN Haodong, Email: haodonglin@hotmail.com

Keywords：

Nerve regeneration; selenium-methylselenocysteine; oxidative stress; inflammatory response; p38 mitogen-activated protein kinase pathway

DOI：

10.7507/1002-1892.202402031

Video：

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Abstract Full text Figures/Tables Video References Cited by

Objective To investigate the feasibility of selenium-methylselenocysteine (SMC) to promote peripheral nerve regeneration and its mechanism of action. Methods Rat Schwann cells RSC96 cells were randomly divided into 5 groups, which were group A (without any treatment, control group), group B (adding 100 μmol/L H₂O₂), group C (adding 100 μmol/L H₂O₂+100 μmol/L SMC), group D (adding 100 μmol/L H₂O₂+200 μmol/L SMC), group E (adding 100 μmol/L H₂O₂+400 μmol/L SMC); the effect of SMC on cell proliferation was detected by MTT method, and the level of oxidative stress was detected by immunofluorescence for free radicals [reactive oxygen species (ROS)] after determining the appropriate dose group. Thirty-six 4-week-old male Sprague Dawley rats were randomly divided into 3 groups, namely, the sham operation group (Sham group), the sciatic nerve injury group (PNI group), and the SMC treatment group (SMC group), with 12 rats in each group; the rats in the PNI group were fed with food and water normally after modelling operation, and the rats in the SMC group were added 0.75 mg/kg SMC to the drinking water every day. At 4 weeks after operation, the sciatic nerves of rats in each group were sampled for neuroelectrophysiological detection of highest potential of compound muscle action potential (CMAP). The levels of inflammatory factors [interleukin 17 (IL-17), IL-6, IL-10 and oxidative stress factors catalase (CAT), superoxide dismutase (SOD), and malondialdehyde (MDA)] were detected by ELISA assay. The luxol fast blue (LFB) staining was used to observe the myelin density, fluorescence intensity of glial fibrillary acidic protein (GFAP) and myelin basic protein (MBP) was observed by immunofluorescence staining, and myelin morphology was observed by transmission electron microscopy with measurement of axon diameter. Western blot was used to detect the protein expressions of p38 mitogen-activated protein kinases (p38MAPK), phosphorylated p38MAPK (p-p38MAPK), heme oxygenase 1 (HO-1), and nuclear factor erythroid 2-related factor 2 (Nrf2). Results MTT assay showed that the addition of SMC significantly promoted the proliferation of RSC96 cells, and the low concentration could achieve an effective effect, so the treatment method of group C was selected for the subsequent experiments; ROS immunofluorescence test showed that group B showed a significant increase in the intensity of ROS fluorescence compared with that of group A, and group C showed a significant decrease in the intensity of ROS fluorescence compared with that of group B (P<0.05). Neuroelectrophysiological tests showed that the highest potential of CMAP in SMC group was significantly higher than that in PNI and Sham groups (P<0.05). ELISA assay showed that the levels of IL-6, IL-17, and MDA in PNI group were significantly higher than those in Sham group, and the levels of IL-10, SOD, and CAT were significantly lower; the levels of IL-6, IL-17, and MDA in SMC group were significantly lower than those in PNI group, and the levels of IL-10, SOD, and CAT were significantly higher (P<0.05). LFB staining and transmission electron microscopy showed that the myelin density and the diameter of axons in the SMC group were significantly higher than those of the PNI group and the Sham group (P<0.05). Immunofluorescence staining showed that the fluorescence intensity of GFAP and MBP in the SMC group were significantly stronger than those in the PNI group and Sham group (P<0.05). Western blot showed that the relative expressions of Nrf2 and HO-1 proteins in the SMC group were significantly higher than those in the PNI group and Sham group, and the ratio of p-p38MAPK/p38MAPK proteins was significantly higher in the PNI group than that in the SMC group and Sham group (P<0.05). Conclusion SMC may inhibit oxidative stress and inflammation after nerve injury by up-regulating the Nrf2/HO-1 pathway, and then inhibit the phosphorylation of p38MAPK pathway to promote the proliferation of Schwann cells, which ultimately promotes the formation of myelin sheaths and accelerates the regeneration of peripheral nerves.

Citation： SONG Jianguo, LIN Haodong. Experimental study on promotion of peripheral nerve regeneration by selenium-methylselenocysteine. Chinese Journal of Reparative and Reconstructive Surgery, 2024, 38(5): 598-607. doi: 10.7507/1002-1892.202402031 Copy

1.	Gao Y, Wang Y, Zhang J, et al. Advancing neural regeneration via adaptable hydrogels: Enriched with Mg²⁺ and silk fibroin to facilitate endogenous cell infiltration and macrophage polarization. Bioact Mater, 2023, 33: 100-113.
2.	Zhang Y, Yi D, Hong Q, et al. Platelet-rich plasma-derived exosomes boost mesenchymal stem cells to promote peripheral nerve regeneration. J Control Release, 2024, 367: 265-282.
3.	Ding Z, Jiang M, Qian J, et al. Role of transforming growth factor-β in peripheral nerve regeneration. Neural Regen Res, 2024, 19(2): 380-386.
4.	黄喜军, 朱庆棠, 江丽, 等. 去细胞异种神经复合同种异体脂肪干细胞修复猕猴周围神经缺损的免疫反应研究. 中国修复重建外科杂志, 2012, 26(8): 993-1000.
5.	Khaled MM, Ibrahium AM, Abdelgalil AI, et al. Regenerative strategies in treatment of peripheral nerve injuries in different animal models. Tissue Eng Regen Med, 2023, 20(6): 839-877.
6.	Cui TW, Lu LF, Cao XD, et al. Exosomes combined with biosynthesized cellulose conduits improve peripheral nerve regeneration. IBRO Neurosci Rep, 2023, 15: 262-269.
7.	Ding W, Li X, Chen H, et al. Nerve merging repair in the replantation of a severed limb with defects in multiple nerves: five cases and long-term follow-up. BMC Surg, 2022, 22(1): 222. doi: 10.1186/s12893-022-01673-1.
8.	Bajaber MA, Hameed A, Hussain G, et al. Chitosan nanoparticles loaded with Foeniculum vulgare extract regulate retrieval of sensory and motor functions in mice. Heliyon, 2024, 10(3): e25414. doi: 10.1016/j.heliyon.2024.e25414.
9.	Zhao G, Zhang T, Li J, et al. Parkin-mediated mitophagy is a potential treatment for oxaliplatin-induced peripheral neuropathy. Am J Physiol Cell Physiol, 2024, 326(1): C214-C228.
10.	Zhang D, Li X, Jing B, et al. α-Asarone attenuates chronic sciatica by inhibiting peripheral sensitization and promoting neural repair. Phytother Res, 2023, 37(1): 151-162.
11.	Liu N, Zhang GX, Zhu CH, et al. Antinociceptive and neuroprotective effect of echinacoside on peripheral neuropathic pain in mice through inhibiting P2X7R/FKN/CX3CR1 pathway. Biomed Pharmacother, 2023, 168: 115675. doi: 10.1016/j.biopha.2023.115675.
12.	Niu S, Wang Z, Yin X, et al. A preliminary predictive model for selenium nutritional status in residents based on three selenium biomarkers. J Trace Elem Med Biol, 2024, 81: 127347. doi: 10.1016/j.jtemb.2023.127347.
13.	García-Esquinas E, Carballo-Casla A, Ortolá R, et al. Blood selenium concentrations are inversely associated with the risk of undernutrition in older adults. Nutrients, 2023, 15(22): 4750. doi: 10.3390/nu15224750.
14.	Wu A, Han M, Ni Z, et al. Multifunctional Sr/Se co-doped ZIF-8 nanozyme for chemo/chemodynamic synergistic tumor therapy via apoptosis and ferroptosis. Theranostics, 2024, 14(5): 1939-1955.
15.	Li H, Wang H, Cui L, et al. The effect of selenium on the proliferation of bovine endometrial epithelial cells in a lipopolysaccharide-induced damage model. BMC Vet Res, 2024, 20(1): 109. doi: 10.1186/s12917-024-03958-4.
16.	Garcia CS, da Rocha MJ, Presa MH, et al. Exploring the antioxidant potential of chalcogen-indolizines throughout in vitro assays. PeerJ, 2024, 12: e17074. doi: 10.7717/peerj.17074.
17.	Fei Y, Li T, Wu R, et al. Se-(methyl)-selenocysteine ameliorates blood-brain barrier disruption of focal cerebral ischemia mice via ferroptosis inhibition and tight junction upregulation in an Akt/GSK3β-dependent manner. Psychopharmacology (Berl), 2024, 241(2): 379-399.
18.	Zhang ZH, Peng JY, Chen YB, et al. Different effects and mechanisms of selenium compounds in improving pathology in Alzheimer’s disease. Antioxidants (Basel), 2023, 12(3): 702. doi: 10.3390/antiox12030702.
19.	Kobayashi H, Suzuki N, Ogra Y. Mutagenicity comparison of nine bioselenocompounds in three Salmonella typhimurium strains. Toxicol Rep, 2018, 5: 220-223.
20.	Li HA, Jia LL, Deng ZY, et al. The effects of selenium on the growth and bone development in the weaned rats. Food Bioscience, 2023, 55: 103018. doi: 10.1016/j.fbio.2023.103018.
21.	Xie Y, Liu Q, Zheng L, et al. Se-methylselenocysteine ameliorates neuropathology and cognitive deficits by attenuating oxidative stress and metal dyshomeostasis in alzheimer model mice. Mol Nutr Food Res, 2018, 62(12): e1800107.Xie Y, Liu Q, Zheng L, et al. Se-methylselenocysteine ameliorates neuropathology and cognitive deficits by attenuating oxidative stress and metal dyshomeostasis in alzheimer model mice. Mol Nutr Food Res, 2018, 62(12): e1800107. doi: 10.1002/mnfr.201800107.
22.	王玺, 王胜, 肖玉周. 脐血间充质干细胞诱导分化为类雪旺细胞修复大鼠坐骨神经损伤的实验研究. 中国修复重建外科杂志, 2015, 29(2): 213-220.
23.	Wu G, Wen X, Kuang R, et al. Roles of macrophages and their interactions with schwann cells after peripheral nerve injury. Cell Mol Neurobiol, 2023, 44(1): 11. doi: 10.1007/s10571-023-01442-5.
24.	Wu X, Jia W. Selenium decipher: Trapping of native selenomethionine-containing peptides in selenium-enriched milk and unveiling the deterioration after ultrahigh-temperature treatment. Anal Chem, 2024, 96(3): 1156-1166.
25.	Yalçın MB, Bora ES, Erbaş O. The effect of liraglutide on axon regeneration and functional recovery after peripheral nerve lesion. Curr Issues Mol Biol, 2024, 46(1): 327-339.
26.	Krause Neto W, Silva WA, Ciena AP, et al. Effects of strength training and anabolic steroid in the peripheral nerve and skeletal muscle morphology of aged rats. Front Aging Neurosci, 2017, 9: 205. doi: 10.3389/fnagi.2017.00205.
27.	Nevinsky GA, Buneva VN, Dmitrenok PS. Multiple sclerosis: Enzymatic cross site-specific recognition and hydrolysis of H3 histone by IgGs against H3, H1, H2A, H2B, H4 histones, myelin basic protein, and DNA. Biomedicines, 2022, 10(10): 2663. doi: 10.3390/biomedicines10102663.
28.	Smirnova EV, Rakitina TV, Ziganshin RH, et al. Comprehensive atlas of the myelin basic protein interaction landscape. Biomolecules, 2021, 11(11): 1628. doi: 10.3390/biom11111628.
29.	Sobierajski E, Lauer G, Czubay K, et al. Development of myelin in fetal and postnatal neocortex of the pig, the European wild boar Sus scrofa. Brain Struct Funct, 2023, 228(3-4): 947-966.
30.	Liao D, Li X, Dong Y, et al. The role of Wnt/β-catenin signaling pathway in the transdifferentiation from periodontal ligament stem cells to Schwann cells. Cell Reprogram, 2017, 19(6): 384-388.
31.	Bulle A, Liu P, Seehra K, et al. Combined KRAS-MAPK pathway inhibitors and HER2-directed drug conjugate is efficacious in pancreatic cancer. Nat Commun, 2024, 15(1): 2503. doi: 10.1038/s41467-024-46811-w.
32.	Deng Y, Yang L, Xie Q, et al. Protein kinase A is involved in neuropathic pain by activating the p38MAPK pathway to mediate spinal cord cell apoptosis. Mediators Inflamm, 2020, 2020: 6420425. doi: 10.1155/2020/6420425.
33.	Ma J, Li T, Yuan H, et al. MicroRNA-29a inhibits proliferation and motility of Schwannoma cells by targeting CDK6. J Cell Biochem, 2018, 119(3): 2617-2626.
34.	Khan A, Shal B, Khan AU, et al. Withametelin, a steroidal lactone, isolated from datura innoxa attenuates STZ-induced diabetic neuropathic pain in rats through inhibition of NF-kB/MAPK signaling. Food Chem Toxicol, 2023, 175: 113742. doi: 10.1016/j.fct.2023.113742.
35.	Caillaud M, Chantemargue B, Richard L, et al. Local low dose curcumin treatment improves functional recovery and remyelination in a rat model of sciatic nerve crush through inhibition of oxidative stress. Neuropharmacology, 2018, 139: 98-116.
36.	Fideles SOM, de Cássia Ortiz A, Buchaim DV, et al. Influence of the neuroprotective properties of quercetin on regeneration and functional recovery of the nervous system. Antioxidants (Basel), 2023, 12(1): 149. doi: 10.3390/antiox12010149.
37.	Brambilla S, Guiotto M, Torretta E, et al. Human platelet lysate stimulates neurotrophic properties of human adipose-derived stem cells better than Schwann cell-like cells. Stem Cell Res Ther, 2023, 14(1): 179. doi: 10.1186/s13287-023-03407-3.
38.	Chen T, Wu Z, Hou Q, et al. The dual angiogenesis effects via Nrf2/HO-1 signaling pathway of melatonin nanocomposite scaffold on promoting diabetic bone defect repair. Int J Nanomedicine, 2024, 19: 2709-2732.
39.	Zhang L, Song W, Li H, et al. 4-octyl itaconate alleviates cisplatin-induced ferroptosis possibly via activating the NRF2/HO-1 signalling pathway. J Cell Mol Med, 2024, 28(7): e18207. doi: 10.1111/jcmm.18207.
40.	Li D, Zhang W, Fu H, et al. DL-3- n-butylphthalide attenuates doxorubicin-induced acute cardiotoxicity via Nrf2/HO-1 signaling pathway. Heliyon, 2024, 10(5): e27644. doi: 10.1016/j.heliyon.2024.e27644.
41.	Qiu C, Li Z, Peng P. Human umbilical cord mesenchymal stem cells protect MC3T3-E1 osteoblasts from dexamethasone-induced apoptosis via induction of the Nrf2-ARE signaling pathway. Regen Ther, 2024, 27: 1-11.
42.	He G, Zhang Y, Feng Y, et al. SBFI26 induces triple-negative breast cancer cells ferroptosis via lipid peroxidation. J Cell Mol Med, 2024, 28(7): e18212. doi: 10.1111/jcmm.18212.
43.	Kerns JM, Walter JS, Patetta MJ, et al. Histological assessment of wallerian degeneration of the rat tibial nerve following crush and transection injuries. J Reconstr Microsurg, 2021, 37(5): 391-404.
44.	Kong YF, Shi W, Zhang DZ, et al. Injectable, antioxidative, and neurotrophic factor-deliverable hydrogel for peripheral nerve regeneration and neuropathic pain relief. Applied Materials Today, 2021, 24: 156. doi: 10.1016/j.apmt.2021.101090.

1. Gao Y, Wang Y, Zhang J, et al. Advancing neural regeneration via adaptable hydrogels: Enriched with Mg²⁺ and silk fibroin to facilitate endogenous cell infiltration and macrophage polarization. Bioact Mater, 2023, 33: 100-113.
2. Zhang Y, Yi D, Hong Q, et al. Platelet-rich plasma-derived exosomes boost mesenchymal stem cells to promote peripheral nerve regeneration. J Control Release, 2024, 367: 265-282.
3. Ding Z, Jiang M, Qian J, et al. Role of transforming growth factor-β in peripheral nerve regeneration. Neural Regen Res, 2024, 19(2): 380-386.
4. 黄喜军, 朱庆棠, 江丽, 等. 去细胞异种神经复合同种异体脂肪干细胞修复猕猴周围神经缺损的免疫反应研究. 中国修复重建外科杂志, 2012, 26(8): 993-1000.
5. Khaled MM, Ibrahium AM, Abdelgalil AI, et al. Regenerative strategies in treatment of peripheral nerve injuries in different animal models. Tissue Eng Regen Med, 2023, 20(6): 839-877.
6. Cui TW, Lu LF, Cao XD, et al. Exosomes combined with biosynthesized cellulose conduits improve peripheral nerve regeneration. IBRO Neurosci Rep, 2023, 15: 262-269.
7. Ding W, Li X, Chen H, et al. Nerve merging repair in the replantation of a severed limb with defects in multiple nerves: five cases and long-term follow-up. BMC Surg, 2022, 22(1): 222. doi: 10.1186/s12893-022-01673-1.
8. Bajaber MA, Hameed A, Hussain G, et al. Chitosan nanoparticles loaded with Foeniculum vulgare extract regulate retrieval of sensory and motor functions in mice. Heliyon, 2024, 10(3): e25414. doi: 10.1016/j.heliyon.2024.e25414.
9. Zhao G, Zhang T, Li J, et al. Parkin-mediated mitophagy is a potential treatment for oxaliplatin-induced peripheral neuropathy. Am J Physiol Cell Physiol, 2024, 326(1): C214-C228.
10. Zhang D, Li X, Jing B, et al. α-Asarone attenuates chronic sciatica by inhibiting peripheral sensitization and promoting neural repair. Phytother Res, 2023, 37(1): 151-162.
11. Liu N, Zhang GX, Zhu CH, et al. Antinociceptive and neuroprotective effect of echinacoside on peripheral neuropathic pain in mice through inhibiting P2X7R/FKN/CX3CR1 pathway. Biomed Pharmacother, 2023, 168: 115675. doi: 10.1016/j.biopha.2023.115675.
12. Niu S, Wang Z, Yin X, et al. A preliminary predictive model for selenium nutritional status in residents based on three selenium biomarkers. J Trace Elem Med Biol, 2024, 81: 127347. doi: 10.1016/j.jtemb.2023.127347.
13. García-Esquinas E, Carballo-Casla A, Ortolá R, et al. Blood selenium concentrations are inversely associated with the risk of undernutrition in older adults. Nutrients, 2023, 15(22): 4750. doi: 10.3390/nu15224750.
14. Wu A, Han M, Ni Z, et al. Multifunctional Sr/Se co-doped ZIF-8 nanozyme for chemo/chemodynamic synergistic tumor therapy via apoptosis and ferroptosis. Theranostics, 2024, 14(5): 1939-1955.
15. Li H, Wang H, Cui L, et al. The effect of selenium on the proliferation of bovine endometrial epithelial cells in a lipopolysaccharide-induced damage model. BMC Vet Res, 2024, 20(1): 109. doi: 10.1186/s12917-024-03958-4.
16. Garcia CS, da Rocha MJ, Presa MH, et al. Exploring the antioxidant potential of chalcogen-indolizines throughout in vitro assays. PeerJ, 2024, 12: e17074. doi: 10.7717/peerj.17074.
17. Fei Y, Li T, Wu R, et al. Se-(methyl)-selenocysteine ameliorates blood-brain barrier disruption of focal cerebral ischemia mice via ferroptosis inhibition and tight junction upregulation in an Akt/GSK3β-dependent manner. Psychopharmacology (Berl), 2024, 241(2): 379-399.
18. Zhang ZH, Peng JY, Chen YB, et al. Different effects and mechanisms of selenium compounds in improving pathology in Alzheimer’s disease. Antioxidants (Basel), 2023, 12(3): 702. doi: 10.3390/antiox12030702.
19. Kobayashi H, Suzuki N, Ogra Y. Mutagenicity comparison of nine bioselenocompounds in three Salmonella typhimurium strains. Toxicol Rep, 2018, 5: 220-223.
20. Li HA, Jia LL, Deng ZY, et al. The effects of selenium on the growth and bone development in the weaned rats. Food Bioscience, 2023, 55: 103018. doi: 10.1016/j.fbio.2023.103018.
21. Xie Y, Liu Q, Zheng L, et al. Se-methylselenocysteine ameliorates neuropathology and cognitive deficits by attenuating oxidative stress and metal dyshomeostasis in alzheimer model mice. Mol Nutr Food Res, 2018, 62(12): e1800107.Xie Y, Liu Q, Zheng L, et al. Se-methylselenocysteine ameliorates neuropathology and cognitive deficits by attenuating oxidative stress and metal dyshomeostasis in alzheimer model mice. Mol Nutr Food Res, 2018, 62(12): e1800107. doi: 10.1002/mnfr.201800107.
22. 王玺, 王胜, 肖玉周. 脐血间充质干细胞诱导分化为类雪旺细胞修复大鼠坐骨神经损伤的实验研究. 中国修复重建外科杂志, 2015, 29(2): 213-220.
23. Wu G, Wen X, Kuang R, et al. Roles of macrophages and their interactions with schwann cells after peripheral nerve injury. Cell Mol Neurobiol, 2023, 44(1): 11. doi: 10.1007/s10571-023-01442-5.
24. Wu X, Jia W. Selenium decipher: Trapping of native selenomethionine-containing peptides in selenium-enriched milk and unveiling the deterioration after ultrahigh-temperature treatment. Anal Chem, 2024, 96(3): 1156-1166.
25. Yalçın MB, Bora ES, Erbaş O. The effect of liraglutide on axon regeneration and functional recovery after peripheral nerve lesion. Curr Issues Mol Biol, 2024, 46(1): 327-339.
26. Krause Neto W, Silva WA, Ciena AP, et al. Effects of strength training and anabolic steroid in the peripheral nerve and skeletal muscle morphology of aged rats. Front Aging Neurosci, 2017, 9: 205. doi: 10.3389/fnagi.2017.00205.
27. Nevinsky GA, Buneva VN, Dmitrenok PS. Multiple sclerosis: Enzymatic cross site-specific recognition and hydrolysis of H3 histone by IgGs against H3, H1, H2A, H2B, H4 histones, myelin basic protein, and DNA. Biomedicines, 2022, 10(10): 2663. doi: 10.3390/biomedicines10102663.
28. Smirnova EV, Rakitina TV, Ziganshin RH, et al. Comprehensive atlas of the myelin basic protein interaction landscape. Biomolecules, 2021, 11(11): 1628. doi: 10.3390/biom11111628.
29. Sobierajski E, Lauer G, Czubay K, et al. Development of myelin in fetal and postnatal neocortex of the pig, the European wild boar Sus scrofa. Brain Struct Funct, 2023, 228(3-4): 947-966.
30. Liao D, Li X, Dong Y, et al. The role of Wnt/β-catenin signaling pathway in the transdifferentiation from periodontal ligament stem cells to Schwann cells. Cell Reprogram, 2017, 19(6): 384-388.
31. Bulle A, Liu P, Seehra K, et al. Combined KRAS-MAPK pathway inhibitors and HER2-directed drug conjugate is efficacious in pancreatic cancer. Nat Commun, 2024, 15(1): 2503. doi: 10.1038/s41467-024-46811-w.
32. Deng Y, Yang L, Xie Q, et al. Protein kinase A is involved in neuropathic pain by activating the p38MAPK pathway to mediate spinal cord cell apoptosis. Mediators Inflamm, 2020, 2020: 6420425. doi: 10.1155/2020/6420425.
33. Ma J, Li T, Yuan H, et al. MicroRNA-29a inhibits proliferation and motility of Schwannoma cells by targeting CDK6. J Cell Biochem, 2018, 119(3): 2617-2626.
34. Khan A, Shal B, Khan AU, et al. Withametelin, a steroidal lactone, isolated from datura innoxa attenuates STZ-induced diabetic neuropathic pain in rats through inhibition of NF-kB/MAPK signaling. Food Chem Toxicol, 2023, 175: 113742. doi: 10.1016/j.fct.2023.113742.
35. Caillaud M, Chantemargue B, Richard L, et al. Local low dose curcumin treatment improves functional recovery and remyelination in a rat model of sciatic nerve crush through inhibition of oxidative stress. Neuropharmacology, 2018, 139: 98-116.
36. Fideles SOM, de Cássia Ortiz A, Buchaim DV, et al. Influence of the neuroprotective properties of quercetin on regeneration and functional recovery of the nervous system. Antioxidants (Basel), 2023, 12(1): 149. doi: 10.3390/antiox12010149.
37. Brambilla S, Guiotto M, Torretta E, et al. Human platelet lysate stimulates neurotrophic properties of human adipose-derived stem cells better than Schwann cell-like cells. Stem Cell Res Ther, 2023, 14(1): 179. doi: 10.1186/s13287-023-03407-3.
38. Chen T, Wu Z, Hou Q, et al. The dual angiogenesis effects via Nrf2/HO-1 signaling pathway of melatonin nanocomposite scaffold on promoting diabetic bone defect repair. Int J Nanomedicine, 2024, 19: 2709-2732.
39. Zhang L, Song W, Li H, et al. 4-octyl itaconate alleviates cisplatin-induced ferroptosis possibly via activating the NRF2/HO-1 signalling pathway. J Cell Mol Med, 2024, 28(7): e18207. doi: 10.1111/jcmm.18207.
40. Li D, Zhang W, Fu H, et al. DL-3- n-butylphthalide attenuates doxorubicin-induced acute cardiotoxicity via Nrf2/HO-1 signaling pathway. Heliyon, 2024, 10(5): e27644. doi: 10.1016/j.heliyon.2024.e27644.
41. Qiu C, Li Z, Peng P. Human umbilical cord mesenchymal stem cells protect MC3T3-E1 osteoblasts from dexamethasone-induced apoptosis via induction of the Nrf2-ARE signaling pathway. Regen Ther, 2024, 27: 1-11.
42. He G, Zhang Y, Feng Y, et al. SBFI26 induces triple-negative breast cancer cells ferroptosis via lipid peroxidation. J Cell Mol Med, 2024, 28(7): e18212. doi: 10.1111/jcmm.18212.
43. Kerns JM, Walter JS, Patetta MJ, et al. Histological assessment of wallerian degeneration of the rat tibial nerve following crush and transection injuries. J Reconstr Microsurg, 2021, 37(5): 391-404.
44. Kong YF, Shi W, Zhang DZ, et al. Injectable, antioxidative, and neurotrophic factor-deliverable hydrogel for peripheral nerve regeneration and neuropathic pain relief. Applied Materials Today, 2021, 24: 156. doi: 10.1016/j.apmt.2021.101090.

Chinese Journal of Reparative and Reconstructive Surgery

Experimental study on promotion of peripheral nerve regeneration by selenium-methylselenocysteine

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