Expression Difference of MicroRNA-155 among Macrophages M1, M2, and Tumor-associated Macrophages_Chinese Journal of Clinical Thoracic and Cardiovascular Surgery

Authors：

WANGXiao-long ^1,2 , CHENXin-tao ¹ , QUGe ¹ , MAHai-bo ¹ , LIYin ¹ ,  QINJian-jun ¹

1. Department of Toracic Surgery, Te Henan Cancer Hospital Afliated to Zhengzhou University, Zhengzhou 540008, P. R. China;
2. Department of Toracic Surgery, Te Huaihe Hospital Henan University, Kaifeng 475000, Henan, P. R. China;

Corresponding author：

QINJian-jun, Email: qinjianjun73@aliyun.com

Keywords：

MicroRNA-155; Macrophages; Tumor-associated macrophages

DOI：

10.7507/1007-4848.20150124

Video：

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Objective To investigate the expressions of microRNA-155 (miR-155) in different phenotypes of activated macrophages. Methods The THP-1 cells underwent polarized activation into M1, M2 or tumor-associated macrophages (TAMs), and the phenotypes were confirmed by flow cytometry. The miR-155 expression was determined by qRt-PCR in M1 macrophages, M2 macrophages and TAMs. Results The miR-155 expression significantly decreased in the M2 macrophages (1.83±0.337, P=0.000), TAMs (1.60±0.233, P=0.000) compared with the M1 (6.580±0.637). The phenotype of TAMs was similar to M2. There was no statistically significant difference between TAMs and M2 macrophages in the expression of miR-155 (P=0.546). Conclusion Different expressions of miR-155 in macrophages M1-type and M2-type may be associated with the differentiation or their cellular functions. The phenotypic characteristics TAMs may transform to macrophages to M2-type. And they may have the same functions.

Citation： WANGXiao-long, CHENXin-tao, QUGe, MAHai-bo, LIYin, QINJian-jun. Expression Difference of MicroRNA-155 among Macrophages M1, M2, and Tumor-associated Macrophages. Chinese Journal of Clinical Thoracic and Cardiovascular Surgery, 2015, 22(5): 476-480. doi: 10.7507/1007-4848.20150124 Copy

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6.	Thai TH, Calado DP, Casola S, et al. Regulation of the germinal center response by microRNA-155. Science, 2007, 316(5824):604-608.
7.	Dorsett Y, McBride KM, Jankovic M, et al. MicroRNA-155 suppresses activation-induced cytidine deaminase-mediated Myc-Igh translocation. Immunity, 2008, 28(5):630-638.
8.	Mantovani A, Sica A, Locati M. Macrophage polarization comes of age. Immunity, 2005, 23(4):344-346.
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12.	Rehman J, Li J, Orschell CM, et al. Peripheral blood "endothelial progenitor cells" are derived from monocyte/macrophages and secrete angiogenic growth factors. Circulation, 2003, 107(8):1164-1169.
13.	Daigneault M, Preston JA, Marriott HM, et al. Te identifcation of markers of macrophage differentiation in PMA-stimulated THP-1 cells and monocyte-derived macrophages. PLoS One, 2010, 5(1):e8668.
14.	Gordon S, Taylor PR. Monocyte and macrophage heterogeneity. Nat Rev Immunol, 2005, 5(12):953-964.
15.	Mantovani A, Sozzani S, Locati M, et al. Macrophage polarization:tumor-associated macrophages as a paradigm for polarized M2 mononuclear phagocytes. Trends in Immunology, 2002, 23(11):549-555.
16.	Llave C, Xie Z, Kasschau KD, et al. Cleavage of Scarecrow-like mRNA targets directed by a class of Arabidopsis miRNA. Science, 2002, 297(5589):2053-2056.
17.	Kluiver J, van den Berg A, de Jong D, et al. Regulation of primicroRNA BIC transcription and processing in Burkitt lymphoma. Oncogene, 2007, 26(26):3769-3776.
18.	Gordon S. Macrophage heterogeneity and tissue lipids. J Clin Invest, 2007, 117(1):89-93.
19.	Gordon S. Alternative activation of macrophages. Nat Rev Immunol, 2003, 3(1):23-35.
20.	Van Ginderachter JA, Movahedi K, Hassanzadeh Ghassabeh G, et al. Classical and alternative activation of mononuclear phagocytes:picking the best of both worlds for tumor promotion. Immunobiology, 2006, 211(6-8):487-501.
21.	O'Connell RM, Taganov KD, Boldin MP, et al. MicroRNA-155 is induced during the macrophage inflammatory response. Proc Natl Acad Sci USA, 2007, 104(5):1604-1609.
22.	Wyckoff J. A paracrine loop between tumor cells and macrophages is required for tumor cell migration in mammary tumors. Cancer Res, 2004(64):7022-7029.
23.	Luo Y, Zhou H, Krueger J, et al. Targeting tumor-associated macrophages as a novel strategy against breast cancer. J Clin Invest, 2006, 116(8):2132-2141.
24.	Murray P J, Wynn TA. Obstacles and opportunities for understanding maerophage polarization. J Leukoc Biol, 201I, 89(4):557-563.

1. Okada H, Kohanbash G, Lotze MT. MicroRNAs in immune regulation——Opportunities for cancer immunotherapy. Int J Biochem Cell Biol, 2010, 42(8):1256-1261.
2. Faraoni I, Antonetti FR, Cardone J, et al. miR-155 gene:a typical multifunctional microRNA. Biochim Biophys Acta, 2009, 1792(6):497-505.
3. Kurowska-Stolarska M, Alivernini S, Ballantine LE, et al. MicroRNA-[155] as a proinflammatory regulator in clinical and experimental arthritis. Proc Natl Acad Sci USA, 2011, 108(27):11193-11198.
4. Vigorito E, Perks KL, Abreu-Goodger C, et al. microRNA-155 regulates the generation of immunoglobulin class-switched plasma cells. Immunity, 2007, 27(6):847-859.
5. Rodriguez A, Vigorito E, Clare S, et al. Requirement of bic/microRNA-[155] for normal immune function. Science, 2007, 316(5824):608-611.
6. Thai TH, Calado DP, Casola S, et al. Regulation of the germinal center response by microRNA-155. Science, 2007, 316(5824):604-608.
7. Dorsett Y, McBride KM, Jankovic M, et al. MicroRNA-155 suppresses activation-induced cytidine deaminase-mediated Myc-Igh translocation. Immunity, 2008, 28(5):630-638.
8. Mantovani A, Sica A, Locati M. Macrophage polarization comes of age. Immunity, 2005, 23(4):344-346.
9. Tsuchiya S, Kobayashi Y, Goto Y, et al. Induction of maturation in cultured human monocytic leukemia cells by a phorbol diester. Cancer Research, 1982, 42(4):1530-1536.
10. Martinez FO, Gordon S, Locati M, et al. Transcriptional profling of the human monocyte-to-macrophage differentiation and polarization:new molecules and patterns of gene expression. J Immunol, 2006, 177(10):7303-7311.
11. Sica A, Mantovani A. Macrophage plasticity and polarization:in vivo veritas. J Clin Invest, 2012, 122(3):787-795.
12. Rehman J, Li J, Orschell CM, et al. Peripheral blood "endothelial progenitor cells" are derived from monocyte/macrophages and secrete angiogenic growth factors. Circulation, 2003, 107(8):1164-1169.
13. Daigneault M, Preston JA, Marriott HM, et al. Te identifcation of markers of macrophage differentiation in PMA-stimulated THP-1 cells and monocyte-derived macrophages. PLoS One, 2010, 5(1):e8668.
14. Gordon S, Taylor PR. Monocyte and macrophage heterogeneity. Nat Rev Immunol, 2005, 5(12):953-964.
15. Mantovani A, Sozzani S, Locati M, et al. Macrophage polarization:tumor-associated macrophages as a paradigm for polarized M2 mononuclear phagocytes. Trends in Immunology, 2002, 23(11):549-555.
16. Llave C, Xie Z, Kasschau KD, et al. Cleavage of Scarecrow-like mRNA targets directed by a class of Arabidopsis miRNA. Science, 2002, 297(5589):2053-2056.
17. Kluiver J, van den Berg A, de Jong D, et al. Regulation of primicroRNA BIC transcription and processing in Burkitt lymphoma. Oncogene, 2007, 26(26):3769-3776.
18. Gordon S. Macrophage heterogeneity and tissue lipids. J Clin Invest, 2007, 117(1):89-93.
19. Gordon S. Alternative activation of macrophages. Nat Rev Immunol, 2003, 3(1):23-35.
20. Van Ginderachter JA, Movahedi K, Hassanzadeh Ghassabeh G, et al. Classical and alternative activation of mononuclear phagocytes:picking the best of both worlds for tumor promotion. Immunobiology, 2006, 211(6-8):487-501.
21. O'Connell RM, Taganov KD, Boldin MP, et al. MicroRNA-155 is induced during the macrophage inflammatory response. Proc Natl Acad Sci USA, 2007, 104(5):1604-1609.
22. Wyckoff J. A paracrine loop between tumor cells and macrophages is required for tumor cell migration in mammary tumors. Cancer Res, 2004(64):7022-7029.
23. Luo Y, Zhou H, Krueger J, et al. Targeting tumor-associated macrophages as a novel strategy against breast cancer. J Clin Invest, 2006, 116(8):2132-2141.
24. Murray P J, Wynn TA. Obstacles and opportunities for understanding maerophage polarization. J Leukoc Biol, 201I, 89(4):557-563.

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Expression Difference of MicroRNA-155 among Macrophages M1, M2, and Tumor-associated Macrophages

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