Objective To investigate antifungal activity in vitro of single or combination of triazole and echinocandin against Aspergillus species. Methods Based on EUCAST protocol,the susceptibilities of 62 isolates of Aspergillus spp. were determined for voriconazole (VRC),itraconazole (ICZ),caspofungin (CAS) and micafungin (MICA). For VRC and ICZ,MIC-0 and MIC-2 were determined. For CAS and MICA,minimum effective concentration (MEC) and MIC-2 were determined. The fractional inhibitory concentration (FIC) was used to evaluate the effect of combination of triazole and echinocandin. Results Indifference was found in 2 isolates of Aspergillus fumigatus in combination of ICZ and CAS or MICA by using MIC-0 endpoint. Synergy was found in all other isolates of Aspergillus spp.With MIC-2 and MEC endpoints,synergy for VRC and CAS,VRC and MICA,ICZ and CAS and ICZ and MICA was found in 16,21,11 and 14 isolates of Aspergillus fumigatus,9,13,9 and 11 isolates of Aspergillus flavus,0,2,1 and 1 isolates of Aspergillus niger,respectively. Conclusion The in vitro sensitivity results of combination of triazole and echinocandin are different with different endpoints. Thus,the efficacy of combination of triazole and echinocandin can not predicted by in vitro sensitivity and should be further confirmed in invasive aspergillosis animal experiments.

Citation： Zhang Ming, Sun Wenkui, Chen Fei, Wu Ting, Yan Wenjie, Su Xin, Shi Yi. In Vitro Antifungal Activity of Single or Combination of Triazole and Echinocandin against Aspergillus Species. Chinese Journal of Respiratory and Critical Care Medicine, 2013, 12(2): 174-178. doi: 10.7507/1671-6205.2014042 Copy