The expression of miR-146 in the lungs of mice after exposed to fine particulate matter and the changes of pulmonary function_Chinese Journal of Respiratory and Critical Care Medicine

Authors：

CHEN Qianhua ¹ ,  HOU Tianfang ¹ , DOU Zhifang ¹ , HUANG Hancong ¹ , SHI Zongming ² , WANG Guangfa ³

1. Department of Respiratory and Critical Care Medicine, Aviation General Hospital, China Medical University, Beijing 100012, P. R. China;
2. Department of Traditional Chinese Medicine, Peking University First Hospital, Beijing 100034, P. R. China;
3. Department of Respiratory and Critical Care Medicine, Peking University First Hospital, Beijing 100034, P. R. China;

Corresponding author：

HOU Tianfang, Email: tf_hou@126.com

Keywords：

MicroRNA; Fine particulate matter; Pulmonary function; Air pollution

DOI：

10.7507/1671-6205.201902010

Video：

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Objective To investigate the changes of lung function after exposure to fine particulate matter (PM_2.5) for 60 days and the expression of miR-146 in mice.Methods Thirty SPF BALB/c mice were treated with noninvasive tracheal instillation of fine particulate matter suspension at different doses (2.5 mg/kg, 5.0 mg/kg, 10.0 mg/kg) for 2 months (two times one week), the blank group and normal saline group were set as control groups. The mice were examined and killed on the next day after the last instillation. Histopathological changes of the lungs, pro-infammatory factors levels in the lung tissues, pulmonary functions and the relative expression of miR-146a and miR-146b in the lung tissues were detected.Results Peak inspiratory flow (PIF) and peak expiratory flow (PEF) were decreased significantly after PM_2.5 exposure, however, lung resistance increased and maximal voluntary ventilation reduced from the general tendency without significant difference. Hematoxylin-eosin stain showed lymphocyte infiltration and macrophage infiltration by phagocytic particles, alveolar spacer widening, inflammatory response increased with the increase of PM_2.5 exposure dosage. Pro-infammatory factors as interleukin-6 in the bronchoalveolar lavage fluid, interferon-γ and tumor necrosis factor-α in the lung homogenate were increased significantly by enzyme linked immunosorbent assay. The relative expressions of miR-146a and miR-146b were up-regulated remarkablely in treatment groups compared to the control group by real-time fluorescence quantitative polymerase chain reaction, which had negative relationships with PIF and PEF.Conclusions The lung function of mice decreases significantly after exposure to fine particulate matter, and the expression of miR-146 is up-regulated.

Citation： CHEN Qianhua, HOU Tianfang, DOU Zhifang, HUANG Hancong, SHI Zongming, WANG Guangfa. The expression of miR-146 in the lungs of mice after exposed to fine particulate matter and the changes of pulmonary function. Chinese Journal of Respiratory and Critical Care Medicine, 2020, 19(6): 586-591. doi: 10.7507/1671-6205.201902010 Copy

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2.	Guo C, Zhang Z, Lau AKH, et al. Effect of long-term exposure to fine particulate matter on lung function decline and risk of chronic obstructive pulmonary disease in Taiwan: a longitudinal, cohort study. Lancet Planet Health, 2018, 2(3): e114-e125.
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4.	Hou TF, Liao JP, Zhang C, et al. Elevated expression of miR-146, miR-139 and miR-340 involved in regulating Th1/Th2 balance with acute exposure of fine particulate matter in mice. Int Immunopharmacol, 2018, 54: 68-77.
5.	Tantucci C, Modina D. Lung function decline in COPD. Int J Chron Obstruct Pulmon Dis, 2012, 7: 95-99.
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8.	IARC (2013) International Agency for Research on Cancer. Outdoor air pollution a leading environmental cause of cancer deaths. https://www.iarc.fr/wp-content/uploads/2018/07/pr221_E.pdf.
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10.	Bollati V, Marinelli B, Apostoli P, et al. Exposure to metal-rich particulate matter modifies the expression of candidate micro RNAs in peripheral blood leukocytes. Environ Health Perspect, 2010, 118(6): 763-768.
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12.	Qiao L, Cai J, Wang H, et al. PM2.5 constituents and hospital emergency-room visits in Shanghai, China. Environ Sci Technol, 2014, 48(17): 10406-10414.
13.	Chuang KJ, Chan CC, Su TC, et al. The effect of urban air pollution on inflammation, oxidative stress, coagulation, and autonomic dysfunction in young adults. Am J Respir Crit Care Med, 2007, 176(4): 370-376.
14.	Luo B, Liu J, Fei G, et al. Impact of probable interaction of low temperature and ambient fine particulate matter on the function of rats alveolar macrophages. Environ Toxicol Pharmacol, 2017, 49: 172-178.
15.	Aztatzi-Aguilar OG, Uribe-Ramírez M, Narváez-Morales J, et al. Early kidney damage induced by subchronic exposure to PM2.5 in rats. Part Fibre Toxicol, 2016, 13(1): 68.
16.	Kirenga BJ, Nantanda R, de Jong C, et al. Lung function of children at three sites of varying ambient air pollution levels in Uganda: a cross sectional comparative study. Int J Environ Res Public Health, 2018, 15(12): 2653.
17.	Ogino K, Nagaoka K, Okuda T, et al. PM2.5-induced airway inflammation and hyperresponsiveness in NC/Nga mice. Environ Toxicol, 2017, 32(3): 1047-1054.
18.	Sonkoly E, Pivarcsi A. Advances in microRNAs: implications for immunity and inflammatory diseases. J Cell Mol Med, 2009, 13(1): 24-38.
19.	Deiters A. Small molecule modifiers of the microRNA and RNA interference pathway. AAPS J, 2010, 12(1): 51-60.
20.	Plank M, Maltby S, Mattes J, et al. Targeting translational control as a novel way to treat inflammatory disease: the emerging role of microRNAs. Clin Exp Allergy, 2013, 43(9): 981-999.
21.	Kapsogeorgou EK, Gourzi VC, Manoussakis MN, et al. Cellular microRNAs (miRNAs) and Sjogren's syndrome: candidate regulators of autoimmune response and autoantigen expression. J Autoimmun, 2011, 37(2): 129-135.
22.	Tang Y, Luo X, Cui H, et al. MicroRNA-146a contributes to abnormal activation of the type I interferon pathway in human lupus by targeting the key signaling proteins. Arthritis Rheum, 2009, 60(4): 1065-1075.
23.	Yamamoto M, Singh A, Sava F, et al. MicroRNA expression in response to controlled exposure to diesel exhaust: attenuation by the antioxidant N-acetylcysteine in a randomized crossover study. Environ Health Perspect, 2013, 121(6): 670-675.

1. Kariisa M, Foraker R, Pennell M, et al. Short- and long-term effects of ambient ozone and fine particulate matter on the respiratory health of chronic obstructive pulmonary disease subjects. Arch Environ Occup Health, 2015, 70(1): 56-62.
2. Guo C, Zhang Z, Lau AKH, et al. Effect of long-term exposure to fine particulate matter on lung function decline and risk of chronic obstructive pulmonary disease in Taiwan: a longitudinal, cohort study. Lancet Planet Health, 2018, 2(3): e114-e125.
3. Vrijens K, Bollati V, Nawrot TS. MicroRNAs as potential signatures of environmental exposure or effect: a systematic review. Environ Health Perspect, 2015, 123(5): 399-411.
4. Hou TF, Liao JP, Zhang C, et al. Elevated expression of miR-146, miR-139 and miR-340 involved in regulating Th1/Th2 balance with acute exposure of fine particulate matter in mice. Int Immunopharmacol, 2018, 54: 68-77.
5. Tantucci C, Modina D. Lung function decline in COPD. Int J Chron Obstruct Pulmon Dis, 2012, 7: 95-99.
6. Mannino DM, Davis KJ. Lung function decline and outcomes in an elderly population. Thorax, 2006, 61(6): 472-477.
7. Tálamo C, de Oca MM, Halbert R, et al. Diagnostic labeling of COPD in five Latin American cities. Chest, 2007, 131(1): 60-67.
8. IARC (2013) International Agency for Research on Cancer. Outdoor air pollution a leading environmental cause of cancer deaths. https://www.iarc.fr/wp-content/uploads/2018/07/pr221_E.pdf.
9. Zhao C, Liao J, Chu W, et al. Involvement of TLR2 and TLR4 and Th1/Th2 shift in inflammatory responses induced by fine ambient particulate matter in mice. Inhal Toxicol, 2012, 24(13): 918-927.
10. Bollati V, Marinelli B, Apostoli P, et al. Exposure to metal-rich particulate matter modifies the expression of candidate micro RNAs in peripheral blood leukocytes. Environ Health Perspect, 2010, 118(6): 763-768.
11. Li XB, Ding Z, Zhang CC, et al. MicroRNA-1228^* inhibit apoptosis in A549 cells exposed to fine particulate matter. Environ Sci Pollut Res Int, 2016, 23(10): 10103-10113.
12. Qiao L, Cai J, Wang H, et al. PM2.5 constituents and hospital emergency-room visits in Shanghai, China. Environ Sci Technol, 2014, 48(17): 10406-10414.
13. Chuang KJ, Chan CC, Su TC, et al. The effect of urban air pollution on inflammation, oxidative stress, coagulation, and autonomic dysfunction in young adults. Am J Respir Crit Care Med, 2007, 176(4): 370-376.
14. Luo B, Liu J, Fei G, et al. Impact of probable interaction of low temperature and ambient fine particulate matter on the function of rats alveolar macrophages. Environ Toxicol Pharmacol, 2017, 49: 172-178.
15. Aztatzi-Aguilar OG, Uribe-Ramírez M, Narváez-Morales J, et al. Early kidney damage induced by subchronic exposure to PM2.5 in rats. Part Fibre Toxicol, 2016, 13(1): 68.
16. Kirenga BJ, Nantanda R, de Jong C, et al. Lung function of children at three sites of varying ambient air pollution levels in Uganda: a cross sectional comparative study. Int J Environ Res Public Health, 2018, 15(12): 2653.
17. Ogino K, Nagaoka K, Okuda T, et al. PM2.5-induced airway inflammation and hyperresponsiveness in NC/Nga mice. Environ Toxicol, 2017, 32(3): 1047-1054.
18. Sonkoly E, Pivarcsi A. Advances in microRNAs: implications for immunity and inflammatory diseases. J Cell Mol Med, 2009, 13(1): 24-38.
19. Deiters A. Small molecule modifiers of the microRNA and RNA interference pathway. AAPS J, 2010, 12(1): 51-60.
20. Plank M, Maltby S, Mattes J, et al. Targeting translational control as a novel way to treat inflammatory disease: the emerging role of microRNAs. Clin Exp Allergy, 2013, 43(9): 981-999.
21. Kapsogeorgou EK, Gourzi VC, Manoussakis MN, et al. Cellular microRNAs (miRNAs) and Sjogren's syndrome: candidate regulators of autoimmune response and autoantigen expression. J Autoimmun, 2011, 37(2): 129-135.
22. Tang Y, Luo X, Cui H, et al. MicroRNA-146a contributes to abnormal activation of the type I interferon pathway in human lupus by targeting the key signaling proteins. Arthritis Rheum, 2009, 60(4): 1065-1075.
23. Yamamoto M, Singh A, Sava F, et al. MicroRNA expression in response to controlled exposure to diesel exhaust: attenuation by the antioxidant N-acetylcysteine in a randomized crossover study. Environ Health Perspect, 2013, 121(6): 670-675.

Chinese Journal of Respiratory and Critical Care Medicine

The expression of miR-146 in the lungs of mice after exposed to fine particulate matter and the changes of pulmonary function

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