Effects of sevoflurane on hyperoxia-induced lung injury in rat_Chinese Journal of Respiratory and Critical Care Medicine

Authors：

OU Gang , LI Qin , WEN Juan ,  CHEN Bin

North Sichuan Medical College, Nanchong, Sichuan 637000, P. R. China;

Corresponding author：

CHEN Bin, Email: 154024348@qq.com

Keywords：

Sevoflurane; hyperoxia-induced lung injury; Rat; Lung protection

DOI：

10.7507/1671-6205.201903087

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Objective To explore the effects of different concentrations of sevoflurane on hyperoxia-induced lung injury in rat.Methods The 72 SD rats were randomly divided into control group C (n=12); sevoflurane inhalation group S, group S contains 5 subgroups (n=12) S_0, S_1.0, S_1.5, S_2.0, S_2.5. Group C wasn’t given any treatment, rats in group S were inhaled 95% oxygen for 48 hours to establish a hyperoxia-induced lung injury model. then rats in each subgroup inhaled sevoflurane at different concentrations of 0%, 1.0%, 1.5%, 2.0% and 2.5% for 1 h respectively, rats in group C were breathe air freely. At the two time points which include inhaled 95% oxygen for 48 hours, and sevoflurane was inhaled for 1 h, blood was collected by the abdominal aorta, then arterial blood was used for blood gas analysis; using enzyme linked immunosorbent assay for the detection of serum tumor necrosis factor (TNF) -α and interleukin (IL) -8 and IL-6 concentration; HE staining was carried out in the right lung, and the pathological changes of lung tissue were observed under light microscope; Wet to dry ratio (W/D) of the left lung was taken.Results After inhalation of 95% oxygen for 48 hours (T₁): compared with the group C, group S of arterial blood gas results suggested that the PaO₂ value decreased, PaCO₂ value increased. The degree of lung tissue injury and the pathological score, TNF-α, IL-8 and IL-6, W/D content increased significantly (P < 0.05), there was no significant difference between the S₀ to S_2.5 groups; After treatment with sevoflurane for 1 h (T₂): compared with the group C, group S of arterial blood gas results suggested that the PaO₂ value decreased, PaCO₂ value increased. the degree of lung tissue injury and the pathological score, TNF-α, IL-8 and IL-6, W/D content increased significantly (P < 0.05); Compared with before sevoflurane treatment, the PaO₂ value increased, PaCO₂ value decreased, TNF-α, IL-8 and IL-6, W/D content decreased, pathological score decreased in group S_1.0 to S_2.5 (P <0.05), but there was no significant difference in group S₀; After treatment with sevoflurane, compared with S_2.0 group, the PaO₂ value decreased, PaCO₂ value increased, TNF-, IL-8 and IL-6, W/D content increased, pathological score increased in the group S_1.0 and S_1.5 (P < 0.05), but there was no significant difference in group S_2.5.Conclusion Sevoflurane can effectively reduce the degree of lung injury caused by hyperoxia in rats especially when the concentration is 2%.

Citation： OU Gang, LI Qin, WEN Juan, CHEN Bin. Effects of sevoflurane on hyperoxia-induced lung injury in rat. Chinese Journal of Respiratory and Critical Care Medicine, 2020, 19(3): 281-286. doi: 10.7507/1671-6205.201903087 Copy

1.	Fukumoto J, Fukumoto I, Parthasarathy PT, et al. NLRP3 deletion protects from hyperoxia-induced acute lung injury. Am J Physiol Cell Physiol, 2013, 305(2): C182-C189.
2.	Ferrando C, Aguilar G, Piqueras L, et al. Sevoflurane, but not propofol, reduces the lung inflammatory response and improves oxygenation in an acute respiratory distress syndrome model: a randomised laboratory study. Eur J Anaesthesiol, 2013, 30(8): 455-463.
3.	Luo C, Yuan D, Zhao W, et al. Sevoflurane ameliorates intestinal ischemia-reperfusion-induced lung injury by inhibiting the synergistic action between mast cell activation and oxidative stress. Mol Med Rep, 2015, 12(1): 1082-1090.
4.	石敏, 金国萍, 刘庆辉, 等. 高氧性急性肺损伤大鼠模型的建立与评价. 中国呼吸与危重监护杂志, 2011, 10(2): 144-147.
5.	Blackwell TS, Hipps AN, Yamamoto Y, et al. NF-kappaB signaling in fetal lung macrophages disrupts airway morphogenesis. J Immunol, 2011, 187(5): 2740-2747.
6.	Kim YE, Park WS, Sung DK, et al. Intratracheal transplantation of mesenchymal stem cells simultaneously attenuates both lung and brain injuries in hyperoxic newborn rats. Pediatr Res, 2016, 80(3): 415-424.
7.	Idriss HT, Naismith JH. TNF alpha and the TNF receptor superfamily: structure-function relationship(s). Microsc Res Tech, 2000, 50(3): 184-195.
8.	陈玉强, 房学东, 王跃生, 等. 半胱氨酸蛋白酶-8 在大鼠小肠缺血再灌注后 TNF-α 诱导肺损伤中的作用. 中国老年学杂志, 2011, 31(3): 470-471.
9.	Watanabe K, Iwahara C, Nakayama H, et al. Sevoflurane suppresses tumour necrosis factor-alpha-induced inflammatory responses in small airway epithelial cells after anoxia/reoxygenation. Br J Anaesth, 2013, 110(4): 637-645.
10.	Allen TC, Kurdowska A. Interleukin 8 and acute lung injury. Arch Pathol Lab Med, 2014, 138(2): 266-269.
11.	Miller EJ, Cohen AB, Nagao S, et al. Elevated levels of NAP-1/interleukin-8 are present in the airspaces of patients with the adult respiratory distress syndrome and are associated with increased mortality. Am Rev Respir Dis, 1992, 146(2): 427-432.
12.	Birukova AA, Tian Y, Meliton A, et al. Stimulation of Rho signaling by pathologic mechanical stretch is a "second hit" to Rho-independent lung injury induced by IL-6. Am J Physiol Lung Cell Mol Physiol, 2012, 302(9): L965-L975.
13.	麦振华, 姚华国, 张媛莉, 等. 血管生成素-1 对脓毒症小鼠肺血管通透性的影响. 中国急救医学, 2013, 33(5): 399-401.
14.	Yang Y, Wang WF, Li YH, et al. Sevoflurane attenuates ventilatorinduced lung injury by regulating cPLA2 expression. Mol Med Rep, 2018, 18(3): 2923-2928.
15.	O'Gara B, Talmor D. Lung protective properties of the volatile anesthetics. Intensive Care Med, 2016, 42(9): 1487-1489.
16.	Ishibe Y, Gui X, Uno H, et al. Effect of sevoflurane on hypoxic pulmonary vasoconstriction in the perfused rabbit lung. Anesthesiology, 1993, 79(6): 1348-1353.
17.	Rodriguez-Gonzalez R, Baluja A, Veiras DRS, et al. Effects of sevoflurane postconditioning on cell death, inflammation and TLR expression in human endothelial cells exposed to LPS. J Transl Med, 2013, 11: 87.
18.	Sun XJ, Li XQ, Wang XL, et al. Sevoflurane inhibits nuclear factor-kappaB activation in lipopolysaccharide-induced acute inflammatory lung injury via toll-like receptor 4 signaling. PLoS One, 2015, 10(4): e122752.
19.	Wang L, Ye Y, Su HB, et al. The anesthetic agent sevoflurane attenuates pulmonary acute lung injury by modulating apoptotic pathways. Braz J Med Biol Res, 2017, 50(3): e5747.

1. Fukumoto J, Fukumoto I, Parthasarathy PT, et al. NLRP3 deletion protects from hyperoxia-induced acute lung injury. Am J Physiol Cell Physiol, 2013, 305(2): C182-C189.
2. Ferrando C, Aguilar G, Piqueras L, et al. Sevoflurane, but not propofol, reduces the lung inflammatory response and improves oxygenation in an acute respiratory distress syndrome model: a randomised laboratory study. Eur J Anaesthesiol, 2013, 30(8): 455-463.
3. Luo C, Yuan D, Zhao W, et al. Sevoflurane ameliorates intestinal ischemia-reperfusion-induced lung injury by inhibiting the synergistic action between mast cell activation and oxidative stress. Mol Med Rep, 2015, 12(1): 1082-1090.
4. 石敏, 金国萍, 刘庆辉, 等. 高氧性急性肺损伤大鼠模型的建立与评价. 中国呼吸与危重监护杂志, 2011, 10(2): 144-147.
5. Blackwell TS, Hipps AN, Yamamoto Y, et al. NF-kappaB signaling in fetal lung macrophages disrupts airway morphogenesis. J Immunol, 2011, 187(5): 2740-2747.
6. Kim YE, Park WS, Sung DK, et al. Intratracheal transplantation of mesenchymal stem cells simultaneously attenuates both lung and brain injuries in hyperoxic newborn rats. Pediatr Res, 2016, 80(3): 415-424.
7. Idriss HT, Naismith JH. TNF alpha and the TNF receptor superfamily: structure-function relationship(s). Microsc Res Tech, 2000, 50(3): 184-195.
8. 陈玉强, 房学东, 王跃生, 等. 半胱氨酸蛋白酶-8 在大鼠小肠缺血再灌注后 TNF-α 诱导肺损伤中的作用. 中国老年学杂志, 2011, 31(3): 470-471.
9. Watanabe K, Iwahara C, Nakayama H, et al. Sevoflurane suppresses tumour necrosis factor-alpha-induced inflammatory responses in small airway epithelial cells after anoxia/reoxygenation. Br J Anaesth, 2013, 110(4): 637-645.
10. Allen TC, Kurdowska A. Interleukin 8 and acute lung injury. Arch Pathol Lab Med, 2014, 138(2): 266-269.
11. Miller EJ, Cohen AB, Nagao S, et al. Elevated levels of NAP-1/interleukin-8 are present in the airspaces of patients with the adult respiratory distress syndrome and are associated with increased mortality. Am Rev Respir Dis, 1992, 146(2): 427-432.
12. Birukova AA, Tian Y, Meliton A, et al. Stimulation of Rho signaling by pathologic mechanical stretch is a "second hit" to Rho-independent lung injury induced by IL-6. Am J Physiol Lung Cell Mol Physiol, 2012, 302(9): L965-L975.
13. 麦振华, 姚华国, 张媛莉, 等. 血管生成素-1 对脓毒症小鼠肺血管通透性的影响. 中国急救医学, 2013, 33(5): 399-401.
14. Yang Y, Wang WF, Li YH, et al. Sevoflurane attenuates ventilatorinduced lung injury by regulating cPLA2 expression. Mol Med Rep, 2018, 18(3): 2923-2928.
15. O'Gara B, Talmor D. Lung protective properties of the volatile anesthetics. Intensive Care Med, 2016, 42(9): 1487-1489.
16. Ishibe Y, Gui X, Uno H, et al. Effect of sevoflurane on hypoxic pulmonary vasoconstriction in the perfused rabbit lung. Anesthesiology, 1993, 79(6): 1348-1353.
17. Rodriguez-Gonzalez R, Baluja A, Veiras DRS, et al. Effects of sevoflurane postconditioning on cell death, inflammation and TLR expression in human endothelial cells exposed to LPS. J Transl Med, 2013, 11: 87.
18. Sun XJ, Li XQ, Wang XL, et al. Sevoflurane inhibits nuclear factor-kappaB activation in lipopolysaccharide-induced acute inflammatory lung injury via toll-like receptor 4 signaling. PLoS One, 2015, 10(4): e122752.
19. Wang L, Ye Y, Su HB, et al. The anesthetic agent sevoflurane attenuates pulmonary acute lung injury by modulating apoptotic pathways. Braz J Med Biol Res, 2017, 50(3): e5747.

Chinese Journal of Respiratory and Critical Care Medicine

Effects of sevoflurane on hyperoxia-induced lung injury in rat

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