Experimental Study on the Protective Effect of Glucosamine on Chondrocyte during Hemarthrosis_West China Medical Journal

Authors：

ZHANG Shiyang ¹ ,  ZHANG Minghua ² , JIANG Dianming ¹ , ZHAO Zenghui ¹ , LI Weichao ¹ , QIAO Bo ¹

1. Department of Orthopedics Surgery, the First Affiliated Hospital; 2. The Affiliated Yongchuan Hospital; Chongqing Medical University, Chongqing 400016, P. R. China;

Corresponding author：

ZHANG Minghua, Email: zhang-shiyang@qq.com

Keywords：

氨基葡萄糖; 关节腔积血; 软骨细胞; 兔

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目的　观察盐酸氨基葡萄糖对兔关节腔积血造成的软骨损伤的保护作用。方法　成年新西兰大白兔50只，随机分为A、B、C、D、E共5组，每组10只。A、B、C、D组采用自体静脉血4 mL右侧关节腔内注射建立关节腔积血模型。建模后，A、B、C组分别给予25、82.5、500 mg/kg的盐酸氨基葡萄糖灌胃，1次/d；D组为阳性对照组，以6 mL/kg蒸馏水灌胃，1次/d；E组为阴性对照组，以6 mL/kg蒸馏水灌胃，1次/d。灌胃8周后取材。肉眼大体观察，光镜下进行Mankin评分及沙红O染色观察，评估软骨损伤程度；采用免疫组织化学法测定Ⅱ型胶原蛋白（COL Ⅱ）表达情况。结果　软骨大体观察和沙红O染色组织学观察显示，A、B、C组与D组、E组之间差异均有统计学意义（P＜0.05）。免疫组织化学提示，A、B、C组COL Ⅱ表达明显高于D组（P＜0.05）。结论　盐酸氨基葡萄糖对兔关节腔积血导致的软骨损伤有一定的保护治疗作用，在防治关节腔积血导致的软骨损伤的发生发展方面，具有一定的作用。

Citation： ZHANG Shiyang,ZHANG Minghua,JIANG Dianming,ZHAO Zenghui,LI Weichao,QIAO Bo. Experimental Study on the Protective Effect of Glucosamine on Chondrocyte during Hemarthrosis. West China Medical Journal, 2012, 27(12): 1788-1791. doi: Copy

1.	Roosendaal G, Vianen ME, Marx JJ, et al. Blood-induced joint damage: a human in vitro study[J]. Arthritis Rheum, 1999, 42(5): 1025-1032.
2.	Lohmander LS, injury RL. Surgery and osteoarthrosis: truth or Consequences[J]. Acta Orthop Scand, 1994, 65(2): 605-609.
3.	Da CC, Dowless GV. Glucosamine sulfate for osteoarthritis[J]. Ann Pharmaeother, 1998, 32(5): 580-587.
4.	Roosendaal G, Tekoppele JM, Vianen ME, et al. Blood-induced joint damage: a canine in vitro study[J]. Arthritis Rheum, 1999, 42(5): 1033-1039.
5.	Lipowitz AJ, Wong PL, Stevens JB. Synovial membrane changes after experimental transection of the cranial cruciate ligament in dogs[J]. Am J Vet Res, 1985, 46(5): 1166-1170.
6.	Mankin HJ, Dorfman H, Lippiello L, et al. Biochemical and metabolic abnormalities in articular cartilage from osteo-arthritic human hips. II. Correlation of morphology with biochemical and metabolic data[J]. J Bone Joint Surg Am, 1971, 53(3): 523-537.
7.	Michel JJ, Goris R, Marieke E, et al. Immature articular cartilage is more susceptible to blood-induced damage than mature articular cartilage:An in vivo animal study[J]. Arthritis Rheum, 2010, 48(2): 396-403.
8.	Valentino LA. Blood-induced joint disease: the pathophysiology of hemophilic arthropathy[J]. J Thromb Haemost, 2010, 8(9): 1895-1902.
9.	张继春, 陈金栋, 陈百成, 等. 兔关节内积血对关节软骨影响的实验研究[J]. 中国康复医学杂志, 2007, 22(1): 31-33.
10.	Hakobyan N. Kazarian T’valentino LA.Synovitis in a murine model of human factor Ⅷdeficiency[J]. Haemophilia, 2005, 11(3): 227-232.
11.	Tan AH, Mitra AK, Chang PC, et al. Assessment of blood-induced cartilage damage in rabbit knees using scanning electron microscopy[J]. J Orthop Surg (Hong Kong), 2004, 12(2): 199-204.
12.	Hakobyan N, Kazarian T, Valentino LA. Synovitis in a murine model of human factor Ⅷ deficiency[J]. Haemophilia, 2005, 11(3): 227-232.
13.	傅欣, 林霖, 魏学磊, 等. 氨基葡萄糖和硫酸软骨素联合用药对兔骨关节炎治疗作用实验研究[J]. 中国运动医学杂志, 2008, 22(5): 588-592.
14.	Piperno M, Reboul P, Hellio Le Graverand MP, et al. Glucosamine sulfate modulates dysregulated activities of human osteoarthritic chondrocytes in vitro[J]. Osteoarthritis Cartilage, 2000, 8(3): 207-212.
15.	Nakamura H, Shibakawa A, Tanaka M, et al. Effects of glucosamine hydrochloride on the production of prostaglandin E2, nitric oxide and metalloproteases by chondrocytes and synoviocytes in osteoarthritis[J]. Clin Exp Rheumatol, 2004, 22(3): 293-299.
16.	杨南萍, 李江涛, 陈永涛, 等. 多中心随机双盲双模拟阳性药平行对照临床研究:评价国产盐酸氨基葡萄糖片在治疗骨关节炎中的有效性和安全性[J]. 华西医学, 2005, 20(4): 54-56.
17.	Bassleer C, Rovati L, Franchimont P. Stimulation of proteoglycan production by glucosamine sulfate in chondrocytes isolated from human osteoarthritic articular cartilage in vitro[J]. Osteoarthritis Cartilage, 1998, 6(6): 427-434.
18.	Qiu GX, Gao SN, Giacovelli G, et al. Efficacy and safety of glucosamine sulfate versus ibuprofen in patients with knee osteoarthritis[J]. Arzneimittelforschung, 1998, 48(5): 469-474.
19.	王晓滨, 黄公怡, 薛庆云. 硫酸氨基葡萄糖抑制白介素1β诱导的人骨关节炎软骨细胞合成一氧化氮研究[J]. 中华老年医学杂志, 2012, 31(2): 140-143.
20.	Largo R, Alvarez-Soria MA, Díez-Ortego I, et al. Glucosamine inhibits IL-1beta-induced NFkappaB activation in human osteoarthritic chondrocytes[J]. Osteoarthritis Cartilage, 2003, 11(4): 290-298.
21.	Huser CA, Davies ME. Effect of a glucosamine derivative on impact-induced chondrocyte apoptosis in vitro. A preliminary report[J]. Osteoarthritis Cartilage, 2008, 16(1): 125-128.

1. 　Roosendaal G, Vianen ME, Marx JJ, et al. Blood-induced joint damage: a human in vitro study[J]. Arthritis Rheum, 1999, 42(5): 1025-1032.
2. 　Lohmander LS, injury RL. Surgery and osteoarthrosis: truth or Consequences[J]. Acta Orthop Scand, 1994, 65(2): 605-609.
3. 　Da CC, Dowless GV. Glucosamine sulfate for osteoarthritis[J]. Ann Pharmaeother, 1998, 32(5): 580-587.
4. 　Roosendaal G, Tekoppele JM, Vianen ME, et al. Blood-induced joint damage: a canine in vitro study[J]. Arthritis Rheum, 1999, 42(5): 1033-1039.
5. 　Lipowitz AJ, Wong PL, Stevens JB. Synovial membrane changes after experimental transection of the cranial cruciate ligament in dogs[J]. Am J Vet Res, 1985, 46(5): 1166-1170.
6. 　Mankin HJ, Dorfman H, Lippiello L, et al. Biochemical and metabolic abnormalities in articular cartilage from osteo-arthritic human hips. II. Correlation of morphology with biochemical and metabolic data[J]. J Bone Joint Surg Am, 1971, 53(3): 523-537.
7. 　Michel JJ, Goris R, Marieke E, et al. Immature articular cartilage is more susceptible to blood-induced damage than mature articular cartilage:An in vivo animal study[J]. Arthritis Rheum, 2010, 48(2): 396-403.
8. 　Valentino LA. Blood-induced joint disease: the pathophysiology of hemophilic arthropathy[J]. J Thromb Haemost, 2010, 8(9): 1895-1902.
9. 　张继春, 陈金栋, 陈百成, 等. 兔关节内积血对关节软骨影响的实验研究[J]. 中国康复医学杂志, 2007, 22(1): 31-33.
10. 　Hakobyan N. Kazarian T’valentino LA.Synovitis in a murine model of human factor Ⅷdeficiency[J]. Haemophilia, 2005, 11(3): 227-232.
11. 　Tan AH, Mitra AK, Chang PC, et al. Assessment of blood-induced cartilage damage in rabbit knees using scanning electron microscopy[J]. J Orthop Surg (Hong Kong), 2004, 12(2): 199-204.
12. 　Hakobyan N, Kazarian T, Valentino LA. Synovitis in a murine model of human factor Ⅷ deficiency[J]. Haemophilia, 2005, 11(3): 227-232.
13. 　傅欣, 林霖, 魏学磊, 等. 氨基葡萄糖和硫酸软骨素联合用药对兔骨关节炎治疗作用实验研究[J]. 中国运动医学杂志, 2008, 22(5): 588-592.
14. 　Piperno M, Reboul P, Hellio Le Graverand MP, et al. Glucosamine sulfate modulates dysregulated activities of human osteoarthritic chondrocytes in vitro[J]. Osteoarthritis Cartilage, 2000, 8(3): 207-212.
15. 　Nakamura H, Shibakawa A, Tanaka M, et al. Effects of glucosamine hydrochloride on the production of prostaglandin E2, nitric oxide and metalloproteases by chondrocytes and synoviocytes in osteoarthritis[J]. Clin Exp Rheumatol, 2004, 22(3): 293-299.
16. 　杨南萍, 李江涛, 陈永涛, 等. 多中心随机双盲双模拟阳性药平行对照临床研究:评价国产盐酸氨基葡萄糖片在治疗骨关节炎中的有效性和安全性[J]. 华西医学, 2005, 20(4): 54-56.
17. 　Bassleer C, Rovati L, Franchimont P. Stimulation of proteoglycan production by glucosamine sulfate in chondrocytes isolated from human osteoarthritic articular cartilage in vitro[J]. Osteoarthritis Cartilage, 1998, 6(6): 427-434.
18. 　Qiu GX, Gao SN, Giacovelli G, et al. Efficacy and safety of glucosamine sulfate versus ibuprofen in patients with knee osteoarthritis[J]. Arzneimittelforschung, 1998, 48(5): 469-474.
19. 　王晓滨, 黄公怡, 薛庆云. 硫酸氨基葡萄糖抑制白介素1β诱导的人骨关节炎软骨细胞合成一氧化氮研究[J]. 中华老年医学杂志, 2012, 31(2): 140-143.
20. 　Largo R, Alvarez-Soria MA, Díez-Ortego I, et al. Glucosamine inhibits IL-1beta-induced NFkappaB activation in human osteoarthritic chondrocytes[J]. Osteoarthritis Cartilage, 2003, 11(4): 290-298.
21. 　Huser CA, Davies ME. Effect of a glucosamine derivative on impact-induced chondrocyte apoptosis in vitro. A preliminary report[J]. Osteoarthritis Cartilage, 2008, 16(1): 125-128.

West China Medical Journal

Experimental Study on the Protective Effect of Glucosamine on Chondrocyte during Hemarthrosis

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