Antitumor Responses of TAG-72 Redirected T Lymphocytes to Breast Cancer_CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY

Authors：

 XU Hongyong ¹ , DING Rong ² , XU Li ³ , LI Kaizong ⁴

1. Department of Hepatobiliary General Surgery， The 451th Hospital of PLA， Xi’an 710054， Shaanxi Province， China;;
2. Department of Specific Diagnosis， The 451th Hospital of PLA， Xi’an 710054， Shaanxi Province， China;;
3. Institute of Digestive Disease， Xijing Hospital， The Fourth Military Medical University， Xi’an 710032， ShaanxiProvince， China;;
4. Department of Hepatobiliary Surgery， Xijing Hospital， The Fourth Military Medical University， Xi’an 710032， Shaanxi Province， China;

Corresponding author：

XU Hongyong, Email: hongyongxu@sohu.com

Keywords：

Breast cancer; Tumor associated glycoprotein-72; Peripheral blood mononuclear cell

Video：

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Abstract Full text Figures/Tables Video References Cited by

Objective 　To investigate the effect of tumor associated glycoprotein-72 （TAG-72） redirected T lymphocytes on breast cancer cells.
Methods 　Peripheral blood mononuclear cells （PBMCs）were isolated from healthy volunteers. The recombinant vector anti-TAG-72-scFv-CD3ζ-pcDNA 3.0 were transfected into PBMCs by lipofectamineTM2000 （transfection group）， PBMCs transfected with plasmid pcDNA 3.0 as control group. MCF-7 and Bcap37 cells were cocultivated with PBMCs of transfection group and control group， respectively， and antitumor response of G1 block was observed.
Results 　G1 block rate of MCF-7 cells in transfection group was （82.3±6.9）%， which was significantly higher than that in control group 〔（43.4±3.9）%， P＜0.05〕. G1 block rate of Bcap37 cells in transfection group was （51.3±4.7）%， and not differed from that in control group 〔（45.6±2.5）%， P＞0.05〕.
Conclusion 　TAG-72 redirected T lymphocytes can inhibit the cell proliferation of TAG-72 positive breast cancer cells， and it may provide valuable tools for the cellular immunotherapy.

Citation： XU Hongyong,DING Rong,XU Li,LI Kaizong. Antitumor Responses of TAG-72 Redirected T Lymphocytes to Breast Cancer. CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY, 2012, 19(12): 1298-1301. doi: Copy

1.	June CH， Bluestone JA， Nadler LM， et al. The B7 and CD28 receptor families[J]. Immunol Today， 1994， 15(7)： 321-331.
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6.	Hombach AA， Schildgen V， Heuser C， et al. T cell activation by antibody-like immunoreceptors： the position of the binding epitope within the target molecule determines the efficiency of activation of redirected T cells[J]. J Immunol， 2007， 178(7)： 4650-4657.
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10.	Ychou M， Duffour J， Kramar A， et al. Clinical significance and prognostic value of CA72-4 compared with CEA and CA19-9 in patients with gastric cancer[J]. Dis Markers， 2000， 16(3-4)： 105-110.
11.	Wang XB， Zhao BF， Zhao Q， et al. A new recombinant single chain trispecific antibody recruits T lymphocytes to kill CEA (carcinoma embryonic antigen) positive tumor cells in vitroefficiently[J]. J Biochem， 2004， 135(4)： 555-565.
12.	Petersen CC， Petersen MS， Agger R， et al. Accumulation in tumor tissue of adoptively transferred T cells：a comparison between intravenous and intraperitoneal injection[J]. J Immunother， 2006， 29(3)： 241-249.
13.	Takizawa K， Kitani S， Takeuchi F， et al. Enhanced expression of CD69 and CD25 antigen on human peripheral blood mononuclear cells by prolactin[J]. Endocr J， 2005， 52(5)： 635-641.

1. June CH， Bluestone JA， Nadler LM， et al. The B7 and CD28 receptor families[J]. Immunol Today， 1994， 15(7)： 321-331.
2. Kapsogeorgou EK， Moutsopoulos HM， Manoussakis MN. Functional expression of a costimulatory B7.2 (CD86) protein on human salivary gland epithelial cells that interacts with the CD28 receptor， but has reduced binding to CTLA4[J]. J Immunol， 2001， 166(5)： 3107-3113.
3. 徐立，徐宏勇，樊代明. 胃肠道肿瘤靶向抗癌胚抗原单链抗体与T细胞表面受体基因的融合分子构建[J]. 中华消化杂志， 2004， 24(2)： 75-77.
4. 徐宏勇，徐立，李开宗，等. 抗肿瘤相关糖蛋白-72单抗在人乳腺癌中的表达[J]. 中国普外基础与临床杂志， 2006， 13(5)： 540-541.
5. Hombach A， Heuser C， Marquardt T， et al. CD4+ T cells engra-fted with a recombinant immunoreceptor efficiently lyse target cells in a MHC antigen- and Fas-independent fashion[J]. JImmunol， 2001， 167(2)： 1090-1096.
6. Hombach AA， Schildgen V， Heuser C， et al. T cell activation by antibody-like immunoreceptors： the position of the binding epitope within the target molecule determines the efficiency of activation of redirected T cells[J]. J Immunol， 2007， 178(7)： 4650-4657.
7. 徐宏勇，徐立，高建宏，等. 嵌合锚定T细胞致癌胚抗原阳性胃癌细胞的凋亡效应[J]. 中华医学杂志， 2007， 87(15)： 1053-1057.
8. 徐宏勇，徐立，李开宗，等. 嵌合锚定融合分子在T淋巴细胞的荧光表达及检测[J]. 中国普外基础与临床杂志， 2010， 17(10)： 1046-1049.
9. 徐宏勇，徐立，李开宗，等. 癌胚抗原嵌合锚定T细胞对胃癌的体内外抑癌效应观察[J]. 中华外科杂志， 2008， 46(2)： 148-149.
10. Ychou M， Duffour J， Kramar A， et al. Clinical significance and prognostic value of CA72-4 compared with CEA and CA19-9 in patients with gastric cancer[J]. Dis Markers， 2000， 16(3-4)： 105-110.
11. Wang XB， Zhao BF， Zhao Q， et al. A new recombinant single chain trispecific antibody recruits T lymphocytes to kill CEA (carcinoma embryonic antigen) positive tumor cells in vitroefficiently[J]. J Biochem， 2004， 135(4)： 555-565.
12. Petersen CC， Petersen MS， Agger R， et al. Accumulation in tumor tissue of adoptively transferred T cells：a comparison between intravenous and intraperitoneal injection[J]. J Immunother， 2006， 29(3)： 241-249.
13. Takizawa K， Kitani S， Takeuchi F， et al. Enhanced expression of CD69 and CD25 antigen on human peripheral blood mononuclear cells by prolactin[J]. Endocr J， 2005， 52(5)： 635-641.

CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY

Antitumor Responses of TAG-72 Redirected T Lymphocytes to Breast Cancer

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