Study of Relationship Between Hepatitis B Virus Genotypes and Hepatocellular Carcinoma_CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY

Authors：

 HU Zhaohui ¹ , LUO Hua ¹ , YANG Pei ¹ , DENG Wenbin ²

1.Department of Hepatobiliary Surgery, Mianyang Central Hospital, Mianyang 621000, Sichuan Province, China;;
2.Department of Medical Laboratories, Mianyang Central Hospital, Mianyang 621000, Sichuan Province, China;

Corresponding author：

HU Zhaohui , Email: huzhaohui.sc@163.com

Keywords：

Hepatitis B virus; Genotype; Hepatocellular carcinoma; PCR type-specific primers analysis

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Objective To explore the relationship between the HBsAg positive patients suffering from hepatocellular carcinoma (HCC) and HBV DNA genotype. Methods By using PCR type-specific primers combined with sequencing of genotype, we analyzed the genotype of HBV DNA in the serum of 500 patients with positive HBsAg in our hospital. Among them, 150 cases suffered from HCC. Results Genotype B and C were both predominant genotypes in HBsAg positive patients. But in HCC group, the rate of genotype C was 65.33% （98/150）, which was significantly higher than that in non-HCC group (88/350, 25.14%), while genotype B, in contrast, was 28.67% （43/150） and 68.86% （241/350）， χ2=75.45， P lt;0.05. The distribution of HBV DNA genotype B or genotype C in different gender or different age groups were not statistically significantly different in cases of HCC (P gt;0.05). Conclusion Genotype C of HBV DNA is more common in patients with HCC, and maybe there is relationship between genotype C and the occurrence of HCC.

Citation： HU Zhaohui ,LUO Hua,YANG Pei,DENG Wenbin. Study of Relationship Between Hepatitis B Virus Genotypes and Hepatocellular Carcinoma. CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY, 2010, 17(12): 1261-1264. doi: Copy

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12.	Livingston SE, Simonetti JP, McMahon BJ, et al. Hepatitis B virus genotypes in Alaska Native people with hepatocellular carcinoma: preponderance of genotype F [J]. J Infect Dis, 2007; 195(1): 5-11.
13.	Elkady A, Tanaka Y, Kurbanov F, et al. Virological and clinical implication of core promoter C1752/V1753 and T1764/G1766 mutations in hepatitis B virus genotype D infection in Mongolia [J]. J Gastroenterol Hepatol, 2008; 23(3): 474-481.
14.	Shrestha SM, Shrestha S, Shrestha A, et al. High prevalence of hepatitis B virus infection and inferior vena cava obstruction among patients with liver cirrhosis or hepatocellular carcinoma in Nepal [J]. Journal J Gastroenterol Hepatol, 2007; 22(11): 1921-1928.
15.	Kew MC, Kramvis A, Yu MC, et al. Increased hepatocarcinogenic potential of hepatitis B virus genotype A in Bantu-speaking sub-saharan Africans [J]. J Med Virol, 2005; 75(4): 513-521.
16.	Tangkijvanich P, Mahachai V, Komolmit P, et al. Hepatitis B virus genotypes and hepatocellular carcinoma in Thailand [J]. World J Gastroenterol, 2005; 11(15): 2238-2243.
17.	Yu MW, Yeh SH, Chen PJ, et al. Hepatitis B virus genotype and DNA level and hepatocellular carcinoma: a prospective study in men [J]. J Natl Cancer Inst, 2005; 97(4): 265-272.
18.	Ito K, Tanaka Y, Kato M, et al. Comparison of complete sequences of hepatitis B virus genotype C between inactive carriers and hepatocellular carcinoma patients before and after seroconversion [J]. J Gastroenterol, 2007; 42(10): 837-844.
19.	Choi MS, Kim DY, Lee DH, et al. Clinical significance of pre-S mutations in patients with genotype C hepatitis B virus infection [J]. J Viral Hepat, 2007; 14(3): 161-168.
20.	Lin CL, Kao JH. Hepatitis B viral factors and clinical outcomes of chronic hepatitis B [J]. J Biomed Sci, 2008; 15(2): 137-145.
21.	Tong MJ, Blatt LM, Kao JH, et al. Basal core promoter T1762/A1764 and precore A1896 gene mutations in hepatitis B surface antigen-positive hepatocellular carcinoma: a comparison with chronic carriers [J]. Liver Int, 2007; 27(10): 1356-1363.
22.	Shinkai N, Tanaka Y, Ito K, et al. Influence of hepatitis B virus X and core promoter mutations on hepatocellular carcinoma among patients infected with subgenotype C2 [J]. J Clin Microbiol, 2007; 45(10): 3191-3197.
23.	Truong BX, Yano Y, Seo Y, et al. Variations in the core promoter/pre-core region in HBV genotype C in Japanese and Northern Vietnamese patients [J]. J Med Virol, 2007; 79(9): 1293-1304.
24.	Ito K, Tanaka Y, Orito E, et al. T1653 mutation in the box alpha increases the risk of hepatocellular carcinoma in patients with chronic hepatitis B virus genotype C infection [J]. Clin Infect Dis, 2006; 42(1): 1-7.

1. Thakur V, Guptan RC, Kazim SN, et al. Profile, spectrum and significance of HBV genotypes in chronic liver disease patients in the Indian subcontinent [J]. J Gastroenterol Hepatol, 2002; 17(2): 165-170.
2. 胡朝辉，罗华，邓文斌, 等. 四川地区乙型肝炎患者HBV基因型分布特点研究 [J]. 西部医学, 2009; 21（12）: 2114-2115.
3. 中国抗癌协会肝癌专业委员会. 原发性肝癌诊断标准 [J]. 中华肝脏病杂志, 2000; 8(3): 135.
4. Naito H, Hayashi S, Abe K. Rapid and specific Rapid and specific genotyping system for hepatitis B virus corresponding to six major genotypes by PCR using type-specific primers [J]. J Clin Microbiol, 2001; 39(1): 362-364.
5. 黄志强主译. 希夫肝脏病学 [M]. 北京: 化学工业出版社, 2006: 689, 1230.
6. 吴孟超. 肝癌外科治疗的近期进展 [J]. 中国普外基础与临床杂志, 2006; 13（2）: 125-128.
7. 肖开银，彭民浩. 原发性肝癌流行病学研究进展 [J]. 中国普外基础与临床杂志, 2000; 7（4）: 272-274.
8. Lin CL, Chen JD, Liu CJ, et al. Clinicopathological differences between hepatitis B viral genotype B- and C-related resectable hepatocellular carcinoma [J]. J Viral Hepat, 2007; 14(1): 64-69.
9. Gao ZY, Li T, Wang J, et al. Mutations in preS genes of genotype C hepatitis B virus in patients with chronic hepatitis B and hepatocellular carcinoma [J]. J Gastroenterol, 2007; 42(9): 761-768.
10. Wang Z, Tanaka Y, Huang Y, et al. Clinical and virological characteristics of hepatitis B virus subgenotypes Ba, C1, and C2 in China [J]. J Clin Microbiol, 2007; 45(5): 1491-1496.
11. 林旭，徐晓，郑大利, 等. 肝癌患者乙型肝炎病毒全基因组结构分析 [J]. 中华肿瘤杂志, 2004; 26（4）： 213-216.
12. Livingston SE, Simonetti JP, McMahon BJ, et al. Hepatitis B virus genotypes in Alaska Native people with hepatocellular carcinoma: preponderance of genotype F [J]. J Infect Dis, 2007; 195(1): 5-11.
13. Elkady A, Tanaka Y, Kurbanov F, et al. Virological and clinical implication of core promoter C1752/V1753 and T1764/G1766 mutations in hepatitis B virus genotype D infection in Mongolia [J]. J Gastroenterol Hepatol, 2008; 23(3): 474-481.
14. Shrestha SM, Shrestha S, Shrestha A, et al. High prevalence of hepatitis B virus infection and inferior vena cava obstruction among patients with liver cirrhosis or hepatocellular carcinoma in Nepal [J]. Journal J Gastroenterol Hepatol, 2007; 22(11): 1921-1928.
15. Kew MC, Kramvis A, Yu MC, et al. Increased hepatocarcinogenic potential of hepatitis B virus genotype A in Bantu-speaking sub-saharan Africans [J]. J Med Virol, 2005; 75(4): 513-521.
16. Tangkijvanich P, Mahachai V, Komolmit P, et al. Hepatitis B virus genotypes and hepatocellular carcinoma in Thailand [J]. World J Gastroenterol, 2005; 11(15): 2238-2243.
17. Yu MW, Yeh SH, Chen PJ, et al. Hepatitis B virus genotype and DNA level and hepatocellular carcinoma: a prospective study in men [J]. J Natl Cancer Inst, 2005; 97(4): 265-272.
18. Ito K, Tanaka Y, Kato M, et al. Comparison of complete sequences of hepatitis B virus genotype C between inactive carriers and hepatocellular carcinoma patients before and after seroconversion [J]. J Gastroenterol, 2007; 42(10): 837-844.
19. Choi MS, Kim DY, Lee DH, et al. Clinical significance of pre-S mutations in patients with genotype C hepatitis B virus infection [J]. J Viral Hepat, 2007; 14(3): 161-168.
20. Lin CL, Kao JH. Hepatitis B viral factors and clinical outcomes of chronic hepatitis B [J]. J Biomed Sci, 2008; 15(2): 137-145.
21. Tong MJ, Blatt LM, Kao JH, et al. Basal core promoter T1762/A1764 and precore A1896 gene mutations in hepatitis B surface antigen-positive hepatocellular carcinoma: a comparison with chronic carriers [J]. Liver Int, 2007; 27(10): 1356-1363.
22. Shinkai N, Tanaka Y, Ito K, et al. Influence of hepatitis B virus X and core promoter mutations on hepatocellular carcinoma among patients infected with subgenotype C2 [J]. J Clin Microbiol, 2007; 45(10): 3191-3197.
23. Truong BX, Yano Y, Seo Y, et al. Variations in the core promoter/pre-core region in HBV genotype C in Japanese and Northern Vietnamese patients [J]. J Med Virol, 2007; 79(9): 1293-1304.
24. Ito K, Tanaka Y, Orito E, et al. T1653 mutation in the box alpha increases the risk of hepatocellular carcinoma in patients with chronic hepatitis B virus genotype C infection [J]. Clin Infect Dis, 2006; 42(1): 1-7.

CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY

Study of Relationship Between Hepatitis B Virus Genotypes and Hepatocellular Carcinoma

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