Effects of Activities of Na+K+ATPase in Plasma Membranes of Hepatocytes in the Formation of Calcium Bilirubinate Gallstone_CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY

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Department of General Surgery, West China Hospital, Sichuan University, Chengdu 610041;

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Keywords：

Calcium bilirubinate gallstone; Na+K+ATPase; Intracellular calcium of hepatocytes; Bile acids; Rabbit model

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Objective To investigate the effects of activities of Na+K+ATPase in plasma membranes of hepatocytes on the formation of calcium bilirubinate gallstone. MethodsThe research were studied in Xiao’s rabbit models.One hundred and three rabbits were randomly divided into the control group (Con,n=28),simple biliary obstruction group (BO,n=36),biliary obstruction and infection group (BOI,n=39).The activities of Na+K+ATPase,contents of intracellular calcium and bile acids in bile were measured in 3rd,7th,14th and 20th day. ResultsThe activities of Na+K+ATPase,contents of bile acids in bile decreased gradually and the contents of intracelluar calcium of hepatocytes increased continously in all phases of experimental groups compared with control group (P＜0.01).There was more significant changes in BOI group than in BO group (P＜0.05). Conclusion The progressive decrease of activities of Na+K+ATPase has a close relationship with the overloaded intracellular calcium and the continous decrease of bile acids contents in bile in formation of calcium bilirubinate gallstone.Infection can promote above mentioned changes and more stone formation.

Citation： ZHUANG Wen,XIAO Lujia,ZUO Fengqiong,et al.. Effects of Activities of Na+K+ATPase in Plasma Membranes of Hepatocytes in the Formation of Calcium Bilirubinate Gallstone. CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY, 2002, 9(2): 73-75. doi: Copy

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1. 周孝思. 我国胆色素结石成因的研究状况 [J]. 普外临床，1994; 9(4)∶193.
2. 曾勇，肖路加，张尚福. 兔胆红素钙结石成石过程中胆囊免疫球蛋白分泌细胞含量的变化 [J]. 普外基础与临床杂志，1997; 4(2)∶75.
3. Meeda T, Bismuth H, Sherlock DJ, et al. A simple and rapid method for the preparation of plasma membranes [J]. Biochim Biophys Acta, 1983; 731(5)∶115.
4. Jame L, Merion RM, Burtch GD, et al. Properties of Na+K+activated ATPase in rat liver plasma membranes enriched with bile canaliculi [J]. Biochim Biophys Acta, 1975; 401(3)∶59.
5. Schanne FA, Muller C, Endlich K, et al. Liver plasma membrane calcium transport [J]. J Biol Chem, 1986; 261(21)∶9886.
6. Michael H, Garrel T, Decurtins M, et al. Mechanisms and regulations of bile secretion [J]. Hepatology, 1991; 14(3)∶551.
7. Frimmer M, Grant B, Zobeck TS, et al. The transport of bile acids in liver cells [J]. Biochim Biophys Acta, 1988; 947(8)∶75.
8. Chung TY. Calcium and ischemic injury [J]. New Engl J Med, 1986;314(26)∶1670.
9. Victor A, Inan M, Sayek I, et al. Conditions that results in the mobilization and influx of Ca2+ into rat hepatocytes induce the rapid loss of HMGCoA reductase activity that is not reversed by phosphatase treatment [J]. Biochem J, 1990; 269(9)∶373.

CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY

Effects of Activities of Na+K+ATPase in Plasma Membranes of Hepatocytes in the Formation of Calcium Bilirubinate Gallstone

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