Protective Effect of Ischemic Preconditioning on IschemicReperfusion Injury of Rat Liver Graft_CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY

Authors：

TU Bing , YAN Lnan , LIU Zhimin

Department of General Surgery, West China Hospital, Sichuan University, Chengdu 610041;

Corresponding author：

Keywords：

Ischemic preconditioning; Rat; Liver graft; Ischemicreperfusion injury

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Objective To investigate the protective effect of ischemic preconditioning (IP) on ischemicreperfusion injury of rat liver graft. MethodsMale Sprague Dawley rats were used as donors and recipients of orthotopic liver transplantation,the period of cold preservation and anhepatic phase were 100 min and 25 min respectively.Sixtyfour rats were randomly divided into 2 groups (n=32),control group: donor livers were flushed through the portal veins with physiological saline solution containing heparin only before harvested; IP group: before donor livers were harvested,the portal veins and hepatic arteries of them were interrupted for 10 min,and reflow was initiated for another 10 min,then did as control group.One half of each group were used to investigate 1 week survival rate of recipients,and another half of each group were used to take sample of blood and hepatic tissue after 2 hours of reperfusion of liver graft. ResultsOne week survival rate,amount of bile,serum NO and activity of antioxidase were higher in IP group than those in control group(P＜0.05),meanwhile,serum ALT,AST,LDH,TNF and superoxide in hepatic tissue were lower in IP group than those in control group (P＜0.05),and histological findings in IP group showed less injury than those in control group. Conclusion IP could increase production of serum NO,reduce the level of serum TNF and protect rat liver graft from ischemicreperfusion injury.

Citation： TU Bing,YAN Lnan,LIU Zhimin. Protective Effect of Ischemic Preconditioning on IschemicReperfusion Injury of Rat Liver Graft. CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY, 2002, 9(2): 89-92. doi: Copy

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1. Lemasters JJ,Bunzendahl H,Thurman RG.Reperfusion injury to donor livers stored for transplantation.Liver \[J\].Transplant Surg,1995; 1(2)∶124.
2. Bilzer M, Gerbs AL.Preservation injury of the liver:mechanism and novel therapeutic strategies \[J\].J Hepatol,2000; 32(3)∶508.
3. Kamada N,Calne RY. A surgical experience with five hundred thirty liver transplants in the rat \[J\].Surgery,1983; 93(1 Pt 1)∶64.
4. Ploeg RJ,D’Alessandro AM,Knechtle SJ,et al.Risk factors for primary dysfunction after liver transplantation:a multivariate analysis \[J\].Transplantation,1993; 55(4)∶807.
5. Jaeschke H.Preservation injury:mechanisms,prevention and consequences \[J\].J Hepatol, 1996; 25(5)∶774.
6. Murry CE,Jennings RB,Reimer KA.Preconditioning with ischemia: a delay of lethal cell injury in ischemic myocardium \[J\].Circulation,1986; 74(5)∶1124.
7. Lichtman SN,Lemasters JJ.Role of cytokines and cytokineproducing cells in reperfusion injury to the liver.Semin \[J\].Liver Dis,1999; 19(2)∶171.
8. Shibuya H,Ohkohchi N,Tsukamoto S,et al.Tumor necrosis factorinduced superoxidemediated neutrophil accumulation in cold ischemic/reperfused rat liver \[J\].Hepatology,1997; 26(1)∶113.
9. Nishida T,Gatmaitan Z,Che MX,et al.Rat liver canalicular membrane vesicles contain an ATPdependent bile acid transport system \[J\].Proc Natl Acad Sci USA,1991; 88(15)∶6590.
10. Bautista AP,Spitzer JJ. Inhibition of nitric oxide formation in vivo enhances superoxide release by the perfused liver \[J\].Am J Physiol,1994; 266(5 Pt 1)∶G783.

CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY

Protective Effect of Ischemic Preconditioning on IschemicReperfusion Injury of Rat Liver Graft

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