Expressions of Livin,Caspase-3, Bcl-2 in Lung Tissue of NSCLC and their Prognostic Significances_Chinese Journal of Respiratory and Critical Care Medicine

Authors：

ZHENG Chunchen ¹ ,  CHEN Yusheng ¹ , CHEN Xiaoyan ² , LI Hongru. ³

Department of Respiratory Medicine,Fujian Provincial Medical College, Fujian Medical University. Fuzhou, Fujian, 350004, China;

Corresponding author：

CHEN Yusheng, Email: slyyywb@yahoo.com.cn

Keywords：

Non-small cell lung cancer; Livin; Caspase-3; Bcl-2; Prognosis

Video：

Export PDF Favorites Scan Get Citation

Abstract Full text Figures/Tables Video References Cited by

Objective To study the expressions of Livin, Caspase-3 and Bcl-2 in lung tissue of nonsmall cell lung cancer ( NSCLC) , and their relationship with the clinicopathological features and prognosis of NSCLC. Methods The expressions of Livin, Caspase-3 and Bcl-2 proteins were evaluated by immunohistochemical method in 87 NSCLC samples and 40 lung benign tissues. The relationship of their expressions with the clinicopathological features and prognosis of NSCLC were analyzed by Spearman’s Rank correlation and COX Regression. Results More NSCLC tissues showed expression of Livin than lung benign tissues( 72. 41% vs 0. 0% , P = 0. 000 ) , and the expression of Caspase-3 was significantly decreased ( 67. 82% vs 87. 5%, P lt; 0. 05 ) . The proteins of Livin, Caspase-3 and Bcl-2 were detected in the endochylema but none was detected in nucelus. There was no relationship between the expression of each of these proteins and the clinicopathological features of NSCLC such as histologic type, tumor differentiation,lymph node metastasis, TNM stage, the size of tumor, and tumor site. The expression of Livin was correlated with Caspase-3 and Bcl-2 expressions ( r1 = - 0. 260, P = 0. 015; r2 = 0. 351, P = 0. 001) . Livin, Caspase-3 and Bcl-2 were not independent prognostic factors of NSCLC. Conclusions The expression of Livin and Bcl-2 are up-regulated in NSCLC. The expression of Livin is positively correlated with that of Caspase-3 and Bcl-2, they might interact with each other in the carcinogenesis and development of NSCLC. The levels of Livin, BCl-2 and Caspase-3 proteins are not independent factors affecting the prognosis of lung cancer patients.

Citation： ZHENG Chunchen,CHEN Yusheng,CHEN Xiaoyan,LI Hongru.. Expressions of Livin,Caspase-3, Bcl-2 in Lung Tissue of NSCLC and their Prognostic Significances. Chinese Journal of Respiratory and Critical Care Medicine, 2009, 09(3): 224-227. doi: Copy

1.	Crook NE, ClemRJ, Miller LK. An apoptosis inhibiting baculooirus gene with a zine finger like motif. J Virol, 1993, 67: 2168 -2174 .
2.	Ka H, Hunt JS. Temporal and spatial patterns of expression of inhibitors of apoptosis in human placentas. Am J Pathol, 2003, 163:413-422.
3.	Ashhab Y, Alian A, Polliack A, et al. Two splicing variants of a new inhibitor of apoptosis gene with different biological properties and tissue distributiong pattern. FEBS Letters, 2001 , 4: 56 -60 .
4.	Gazzaniga P, Gradilone A, Giuliani L, et al. Expression and prognostic significance of Livin, Survivin and other apoptosis-related genes in the progression of superficial bladder cancer. Ann Oncol,2003, 14: 85-90.
5.	宋滔, 洪金发, 高江平, 等. 前列腺癌组织中凋亡抑制基因Livin表达研究. 中华男科学杂志, 2008, 14: 30-33.
6.	Lin JH, Deng G, Huang Q, et al. KIAP, a novel member of the inhibitor of apoptosis protein family. Biochem Biophys Res Commun, 2000, 279: 820-831.
7.	Vucic D, Stennicke HR, Pisabarro MT, et al. ML-IAP, a novel inhibitor of apoptosis that is preferentially expressed in human melanomas. Curr Biol, 2000, 10: 1359-1366.
8.	Kasof GM, Gomes BC. Livin, a novel inhibitor of apoptosis protein family member. J Biol Chem, 2001, 276: 3238-3246.
9.	Berry N, Jones DB, Smallwood J, et al. The prognostic value of the monoclonal antibodies HMFG1 and HMFG2 in breast cancer. Br J Cancer, 1985, 51: 179-186.
10.	黎军和, 何文静, 和友兼. Survivin 和Livin 在Duke’B 期结直肠癌中的表达及其临床意义. 癌症, 2007, 26: 547-551.
11.	陶晓南, 张晓菊, 陈淼. 凋亡抑制蛋白Livin 和抑癌基因P53 在非小细胞肺癌中的表达及临床病理学意义. 中国组织化学与细胞化学杂志, 2007, 16: 293-296.
12.	李鸿茹, 陈愉生, 陈刚, 等. Livin 在肺癌组织中的表达及与procaspase3的相关性初步探讨. 中国肺癌杂志, 2007, 10: 486-490.
13.	Wang DG, Johnston CF, Sloan JM, et al. Expression of Bcl-2 in lung neuroendocrine tumours: comparison with p53. J Pathol, 1998, 184:247-251.
14.	Swanton E, Savory P, Cosulich S, et al. Bcl-2 regulates a Caspase-3 /Caspase-2 apoptotic cascade in cytosolic extracts. Oncogene, 1999 ,18: 1781-1787.
15.	Zhan Y, van de Water B, Wang Y, et al. The roles of caspase-3 andBcl-2 in chemically-induced apoptosis but not necrosis of renal epithelial cells. Oncogene, 1999, 18: 6505-6512.
16.	He H, Lam M, McCormick TS, et al. Maintenance of calcium homeostasis in the endoplasmic reticulum by Bcl-2 . J Cell Biol,1997, 138: 1219-1228.
17.	Schrantz N, Blanchard DA, Mitenne F, et al. Manganese induces apoptosis of human B cells: Caspase-dependent cell death blocked by Bcl-2. Cell Death Differ, 1999, 6: 445-453.
18.	Planchon SM, Wuerzberger-Davis SM, Pink JJ, et al. Bcl-2 protects against beta-lapachone-mediated Caspase 3 activation and apoptosis in human myeloid leukemia( HL-60) cells. Oncol Rep,1999, 6: 485-492.
19.	Nachmias B, Ashhab Y, Bucholtz V, et al. Caspase-mediated cleavage converts Livin from an antiapoptotic to a proapoptotic factor: implications for drug-resistant melanoma. Cancer Res, 2003 ,63: 6340-6349.
20.	Yoo J, Jung JH, Lee MA, et al. Immunohistochemical analysis of non-small cell lung cancer: correlation with clinical parameters and prognosis. J Korean Med Sci, 2007, 22: 318-325.
21.	Pezzella F, Turley H, Kuzu I, et al. Bcl-2 protein in non-small-cell lung carcinoma. N Engl JMed, 1993, 329: 690-694.
22.	Fontanini G, Vignati S, Bigini D, et al. Bcl-2 protein: a prognostic factor inversely correlated to p53 in non-small-cell lung cancer. Br J Cancer, 1995, 71: 1003-1007.
23.	Walker C, Robertson L, Myskow M, et al. Expression of the Bcl-2 protein in normal and dysplastic bronchial epithelium and in lung carcinomas. Br J Cancer Res, 1995, 72: 164-169.
24.	Nedelcu T, Kubista B, Koller A, et al. Livin and Bcl-2 expression in high-grade osteosarcoma. J Cancer Res Clin Oncol, 2008, 134: 237-244.
25.	Choi J, Hwang YK, Sung KW, et al. Expression of Livin, an antiapoptotic protein, is an independent favourable prognostic factor in childhood acute lymphoblastic leukemia. Blood,2007, 109:471-477.

1. Crook NE, ClemRJ, Miller LK. An apoptosis inhibiting baculooirus gene with a zine finger like motif. J Virol, 1993, 67: 2168 -2174 .
2. Ka H, Hunt JS. Temporal and spatial patterns of expression of inhibitors of apoptosis in human placentas. Am J Pathol, 2003, 163:413-422.
3. Ashhab Y, Alian A, Polliack A, et al. Two splicing variants of a new inhibitor of apoptosis gene with different biological properties and tissue distributiong pattern. FEBS Letters, 2001 , 4: 56 -60 .
4. Gazzaniga P, Gradilone A, Giuliani L, et al. Expression and prognostic significance of Livin, Survivin and other apoptosis-related genes in the progression of superficial bladder cancer. Ann Oncol,2003, 14: 85-90.
5. 宋滔, 洪金发, 高江平, 等. 前列腺癌组织中凋亡抑制基因Livin表达研究. 中华男科学杂志, 2008, 14: 30-33.
6. Lin JH, Deng G, Huang Q, et al. KIAP, a novel member of the inhibitor of apoptosis protein family. Biochem Biophys Res Commun, 2000, 279: 820-831.
7. Vucic D, Stennicke HR, Pisabarro MT, et al. ML-IAP, a novel inhibitor of apoptosis that is preferentially expressed in human melanomas. Curr Biol, 2000, 10: 1359-1366.
8. Kasof GM, Gomes BC. Livin, a novel inhibitor of apoptosis protein family member. J Biol Chem, 2001, 276: 3238-3246.
9. Berry N, Jones DB, Smallwood J, et al. The prognostic value of the monoclonal antibodies HMFG1 and HMFG2 in breast cancer. Br J Cancer, 1985, 51: 179-186.
10. 黎军和, 何文静, 和友兼. Survivin 和Livin 在Duke’B 期结直肠癌中的表达及其临床意义. 癌症, 2007, 26: 547-551.
11. 陶晓南, 张晓菊, 陈淼. 凋亡抑制蛋白Livin 和抑癌基因P53 在非小细胞肺癌中的表达及临床病理学意义. 中国组织化学与细胞化学杂志, 2007, 16: 293-296.
12. 李鸿茹, 陈愉生, 陈刚, 等. Livin 在肺癌组织中的表达及与procaspase3的相关性初步探讨. 中国肺癌杂志, 2007, 10: 486-490.
13. Wang DG, Johnston CF, Sloan JM, et al. Expression of Bcl-2 in lung neuroendocrine tumours: comparison with p53. J Pathol, 1998, 184:247-251.
14. Swanton E, Savory P, Cosulich S, et al. Bcl-2 regulates a Caspase-3 /Caspase-2 apoptotic cascade in cytosolic extracts. Oncogene, 1999 ,18: 1781-1787.
15. Zhan Y, van de Water B, Wang Y, et al. The roles of caspase-3 andBcl-2 in chemically-induced apoptosis but not necrosis of renal epithelial cells. Oncogene, 1999, 18: 6505-6512.
16. He H, Lam M, McCormick TS, et al. Maintenance of calcium homeostasis in the endoplasmic reticulum by Bcl-2 . J Cell Biol,1997, 138: 1219-1228.
17. Schrantz N, Blanchard DA, Mitenne F, et al. Manganese induces apoptosis of human B cells: Caspase-dependent cell death blocked by Bcl-2. Cell Death Differ, 1999, 6: 445-453.
18. Planchon SM, Wuerzberger-Davis SM, Pink JJ, et al. Bcl-2 protects against beta-lapachone-mediated Caspase 3 activation and apoptosis in human myeloid leukemia( HL-60) cells. Oncol Rep,1999, 6: 485-492.
19. Nachmias B, Ashhab Y, Bucholtz V, et al. Caspase-mediated cleavage converts Livin from an antiapoptotic to a proapoptotic factor: implications for drug-resistant melanoma. Cancer Res, 2003 ,63: 6340-6349.
20. Yoo J, Jung JH, Lee MA, et al. Immunohistochemical analysis of non-small cell lung cancer: correlation with clinical parameters and prognosis. J Korean Med Sci, 2007, 22: 318-325.
21. Pezzella F, Turley H, Kuzu I, et al. Bcl-2 protein in non-small-cell lung carcinoma. N Engl JMed, 1993, 329: 690-694.
22. Fontanini G, Vignati S, Bigini D, et al. Bcl-2 protein: a prognostic factor inversely correlated to p53 in non-small-cell lung cancer. Br J Cancer, 1995, 71: 1003-1007.
23. Walker C, Robertson L, Myskow M, et al. Expression of the Bcl-2 protein in normal and dysplastic bronchial epithelium and in lung carcinomas. Br J Cancer Res, 1995, 72: 164-169.
24. Nedelcu T, Kubista B, Koller A, et al. Livin and Bcl-2 expression in high-grade osteosarcoma. J Cancer Res Clin Oncol, 2008, 134: 237-244.
25. Choi J, Hwang YK, Sung KW, et al. Expression of Livin, an antiapoptotic protein, is an independent favourable prognostic factor in childhood acute lymphoblastic leukemia. Blood,2007, 109:471-477.

Next Article
Factors Determining the Health Financing Systems: A Descriptive Systematic Review

Chinese Journal of Respiratory and Critical Care Medicine

Expressions of Livin,Caspase-3, Bcl-2 in Lung Tissue of NSCLC and their Prognostic Significances

Abstract Full text Figures/Tables Video References Cited by

Next Article

Format

Content