Clinical Significance of 8-Isoprostane and IL-6 in Exhaled Breath Condensate of Patients with COPD_Chinese Journal of Respiratory and Critical Care Medicine

Authors：

SUN Jian ¹ , SHEN Juxin ¹ , ZHOU Guozhong ² , WANG Hua ¹

Department of Respiratory Medicine, Shaoxing People’s Hospital. Shaoxing, Zhejiang,312000, ChinaCorresponding Author: SUN Jian, E-mail: 2002sunjian@ 163. com;

Corresponding author：

Keywords：

Chronic obstructive pulmonary disease; Exhaled breath condensate; Interleukin-6; 8-Isoprostane

Video：

Export PDF Favorites Scan Get Citation

Abstract Full text Figures/Tables Video References Cited by

Objective To explore the clinical significance of IL-6 and 8-isoprostane( 8-iso-PG) in exhaled breath condensate( EBC) in chronic obstructive pulmonary disease ( COPD) and smoking. Methods 17 patients with stable COPD and 30 healthy controls( 14 smokers and 16 non-smokers) were included in this study. EBC was collected in all subjects with a self-designed experimental device. The concentrations of 8-iso-PG and IL-6 in EBC were determined by enzyme-linked immunosorbent assay. Results Both8-iso-PG and IL-6 levels significantly increased in the COPD patients [ ( 8. 37±5. 09) pg/mL and ( 4. 62 ±1. 73) pg/mL, respectively] compared with the healthy non-smokers[ ( 5. 23 ±3. 08) pg/mL and ( 3. 09 ±1. 85) pg/mL, respectively] ( both P lt;0. 01) . There was no difference between the COPD patients and the
healthy smokers( both P gt; 0. 05) . The IL-6 level significantly increased in the healthy smokers compared with the non-smokers [ ( 4. 06 ±1. 59) pg/mL vs ( 3. 09 ±1. 85) pg/mL, P lt;0. 05] , but the 8-iso-PG level was similar in the non-smokers and smokers( P gt;0. 05) . Conclusion Airway inflammation and oxide stress are persistent in stable COPD patients and are aggravated by smoking.

Citation： SUN Jian,SHEN Juxin,ZHOU Guozhong,WANG Hua. Clinical Significance of 8-Isoprostane and IL-6 in Exhaled Breath Condensate of Patients with COPD. Chinese Journal of Respiratory and Critical Care Medicine, 2010, 9(2): 137-140. doi: Copy

1.	中华医学会呼吸病学分会慢性阻塞性肺疾病学组. 慢性阻塞性肺疾病诊治指南( 2007 年修订版) . 中华结核和呼吸杂志,2007, 30: 8-17.
2.	Biernacki WA, Kharitonov SA, Barnes PJ. Increased leukotriene B4 and 8-isoprostane in exhaled breath condensate of patients with exacerbations of COPD. Thorax, 2003 , 58: 294-298.
3.	Carpagnano GE, Kharitonov SA, Foschino-Barbaro MP, et al.Supplementary oxygen in healthy subjects and those with COPD increases oxidative stress and airway inflammation. Thorax, 2004 ,59: 1016-1019.
4.	Horváth I, Hunt J, Barnes PJ, et al. Exhaled breath condensate:methodological recommendations and unresolved questions. Eur Respir J, 2005, 26: 523-548 .
5.	乔华, 王广发, 张红, 等. 阻塞性睡眠呼吸暂停低通气综合征患者呼出气冷凝液中白细胞介素6 的研究. 中华结核和呼吸杂志, 2005, 28: 364-367.
6.	Effros RM, Su J, Casaburi R, et al. Utility of exhaled breath condensates in chronic obstructive pulmonary disease: a critical review. Curr Opin Pulm Med, 2005, 11: 135-139 .
7.	Montuschi P. Analysis of exhaled breath condensate in respiratory medicine: methodological aspects and potential clinical applications.Ther Adv Respir Dis, 2007, 1: 5 -23 .
8.	Montuschi P, Barnes PJ, Roberts LJ 2nd. Isoprostanes: markers andmediators of oxidative stress. FASEB J, 2004, 18 : 1791 -1800 .
9.	Kostikas K, Papatheodorou G, Psathakis K, et al. Oxidative stress in expired breath condensate of patients with COPD. Chest, 2003, 124 :1373-1380.
10.	刘翱, 李少莹, 杨伟康, 等. 支气管哮喘患者呼出气冷凝液中8 -异前列腺素水平的研究. 中国呼吸与危重监护杂志, 2009, 8 :337-340.
11.	Walter RE, Wilk JB, Larson MG, et al. Systemic inflammation and COPD: the Framingham Heart Study. Chest, 2008, 133: 19-25.
12.	Carpagnano GE, Kharitonov SA, Foschino-Barbaro MP, et al.Increased inflammatory markers in the exhaled breath condensate of cigarette smokers. Eur Respir J, 2003, 21: 589-593 .
13.	MacNee W. Oxidants /antioxidants and chronic obstructive pulmonary disease: pathogenesis to therapy. Novartis Found Symp,2001, 234: 169-185.
14.	MacNee W. Pulmonary and systemic oxidant /antioxidant imbalance in chronic obstructive pulmonary disease. Proc Am Thorac Soc ,2005, 2: 50-60.

1. 中华医学会呼吸病学分会慢性阻塞性肺疾病学组. 慢性阻塞性肺疾病诊治指南( 2007 年修订版) . 中华结核和呼吸杂志,2007, 30: 8-17.
2. Biernacki WA, Kharitonov SA, Barnes PJ. Increased leukotriene B4 and 8-isoprostane in exhaled breath condensate of patients with exacerbations of COPD. Thorax, 2003 , 58: 294-298.
3. Carpagnano GE, Kharitonov SA, Foschino-Barbaro MP, et al.Supplementary oxygen in healthy subjects and those with COPD increases oxidative stress and airway inflammation. Thorax, 2004 ,59: 1016-1019.
4. Horváth I, Hunt J, Barnes PJ, et al. Exhaled breath condensate:methodological recommendations and unresolved questions. Eur Respir J, 2005, 26: 523-548 .
5. 乔华, 王广发, 张红, 等. 阻塞性睡眠呼吸暂停低通气综合征患者呼出气冷凝液中白细胞介素6 的研究. 中华结核和呼吸杂志, 2005, 28: 364-367.
6. Effros RM, Su J, Casaburi R, et al. Utility of exhaled breath condensates in chronic obstructive pulmonary disease: a critical review. Curr Opin Pulm Med, 2005, 11: 135-139 .
7. Montuschi P. Analysis of exhaled breath condensate in respiratory medicine: methodological aspects and potential clinical applications.Ther Adv Respir Dis, 2007, 1: 5 -23 .
8. Montuschi P, Barnes PJ, Roberts LJ 2nd. Isoprostanes: markers andmediators of oxidative stress. FASEB J, 2004, 18 : 1791 -1800 .
9. Kostikas K, Papatheodorou G, Psathakis K, et al. Oxidative stress in expired breath condensate of patients with COPD. Chest, 2003, 124 :1373-1380.
10. 刘翱, 李少莹, 杨伟康, 等. 支气管哮喘患者呼出气冷凝液中8 -异前列腺素水平的研究. 中国呼吸与危重监护杂志, 2009, 8 :337-340.
11. Walter RE, Wilk JB, Larson MG, et al. Systemic inflammation and COPD: the Framingham Heart Study. Chest, 2008, 133: 19-25.
12. Carpagnano GE, Kharitonov SA, Foschino-Barbaro MP, et al.Increased inflammatory markers in the exhaled breath condensate of cigarette smokers. Eur Respir J, 2003, 21: 589-593 .
13. MacNee W. Oxidants /antioxidants and chronic obstructive pulmonary disease: pathogenesis to therapy. Novartis Found Symp,2001, 234: 169-185.
14. MacNee W. Pulmonary and systemic oxidant /antioxidant imbalance in chronic obstructive pulmonary disease. Proc Am Thorac Soc ,2005, 2: 50-60.

Chinese Journal of Respiratory and Critical Care Medicine

Clinical Significance of 8-Isoprostane and IL-6 in Exhaled Breath Condensate of Patients with COPD

Abstract Full text Figures/Tables Video References Cited by

Format

Content