Research of TAP Induction The Pancreatic Acinar Cells to Release HMGB1 in Rat_CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY

Authors：

WANG Guoliang ¹ , DUI Danhua ¹ , BAI Liang ² , LIU Yao ¹ , TIAN Fei ¹ , WEI Wei ¹

1. Department of Hepatobiliaropancreatic Surgery， Affiliated Hospital of Zunyi Medical College， Zunyi 563003， Guizhou Province， China;;
2. Department of General Surgery， Weihai Municipal Hospital， Weihai 266520， Shandong Province， China;

Corresponding author：

Keywords：

Pancreatic acinar cell; Trypsinogen activation peptide; High mobility group box 1; Ethyl pyruvate; Rat

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Objective 　To explore the secretion law of high mobility group box 1（HMGB1）in rat pancreatic acinar cells induced by trypsin activation peptide（TAP）and release of HMGB1 affected by ethyl pyruvate（EP）.
Methods 　The experiment was performed in 12 SD rats. The pancreatic acinar cells of rats were taken out and then separated into three groups：control group， TAP group， and EP group. TAP was added into TAP group and EP group（keep TAP at a final concentration of 3 nmol/L）， respectively， but EP was added into EP group only （keep EP at a final concentration of 28 mmol/L）. The expressions of HMGB1 mRNA and protein were detected by using real-time quantitative reverse transcription polymerase chain reaction（RT-PCR）or Western blot at 3 h， 6 h， 12 h， and 24 h time point， respectively. The relationship between HMGB1 and TAP action time was explored by rank correlation.
Results 　Compared with control group， the expressions of HMGB1 mRNA and protein were increased with prolongation of the TAP action in TAP group and EP group（P＜0.05）. Compared with TAP group， the expressions of HMGB1 mRNA and protein were decreased in EP group（P＜0.05）. The expressions of HMGB1 mRNA and protein were increased with prolongation of the TAP action（P＜0.05）， and were highest at 12 h time point（P＜0.01）in TAP group. There were positive correlation between the expressions of HMGB1 mRNA and protein and TAP action time（rs=0.971， P＜0.01；rs=0.966， P＜0.01）.
Conclusions 　TAP can induce the release of HMGB1 in pancreatic acinar cells. There is positive relationship between TAP in early stage and HMGB1 in later period of acute pancreatitis. EP can inhibit the release of HMGB1.

Citation： WANG Guoliang,DUI Danhua,BAI Liang,LIU Yao,TIAN Fei,WEI Wei. Research of TAP Induction The Pancreatic Acinar Cells to Release HMGB1 in Rat. CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY, 2012, 19(10): 1074-1078. doi: Copy

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1. 黄莚庭. 急性胰腺炎细胞内早期事件的再认识[J]. 中华肝胆外科杂志， 2006， 12(2)： 75-76.
2. 于秉治，余承高，陈栋梁，等. 医用生物化学[M]. 北京：中国协和医科大学出版社， 2004： 55-57.
3. Hyvönen MT， Herzig KH， Sinervirta R， et al. Activated polyamine catabolism in acute pancreatitis： alpha-methylated polyamine analogues prevent trypsinogen activation and pancreatitis-associated mortality[J]. Am J Pathol， 2006， 168(1)： 115-122.
4. Goodwin GH， Sanders C， Johns EW. A new group of chromatin-associated proteins with a high content of acidic and basic amino acdis[J]. Eur J Biochem， 1973， 38(1)： 14-19.
5. 姚咏明，盛志勇. 高迁移率族蛋白-1在脓毒症发病中的作用与意义[J]. 解放军医学杂志， 2002 ， 27(9)： 753-756.
6. 栾正刚，何忠野，张成，等. 丙酮酸乙酯对重症急性胰腺炎大鼠肠黏膜HMGB1表达的影响[J]. 中国普通外科杂志， 2008， 17(3)： 214-218.
7. 陈诗书，孔良曼，章有章，等. 医学生物化学[M]. 上海：复旦大学出版社， 1999： 43-44.
8. 高军，张淑文，李非. 胰蛋白酶原激活肽在大鼠实验性急性胰腺炎中的产生及意义[J]. 中国普外基础与临床杂志， 2001，8(5)： 301-306.
9. 张明威，王欣，张静. 胰蛋白酶原激活肽与急性胰腺炎严重程度的相关性研究[J]. 临床肝胆病杂志， 2007， 23(6)： 448-449.
10. 朱峰，龙锦，何忠野，等. 急性坏死性胰腺炎大鼠胰腺高迁移率族蛋白-1的表达及意义[J]. 中国普通外科杂志， 2007， 16(5)： 418-421.
11. Andersson U， Wang H， Palmblad K， et al. High mobility group 1 protein (HMG-1) stimulates proinflammatory cytokine synthesis in human monocytes[J]. J Exp Med， 2000， 192(4)： 565-570.
12. Rouhiainen A， Imai S， Rauvala H， et al. Occurrence of amphoterin (HMG1) as an endogenous protein of human platelets that is exported to the cell surface upon platelet activation[J]. Thromb Haemost， 2000， 84(6)： 1087-1094.
13. 杨智勇，王春友，熊炯祈，等. 重症急性胰腺炎大鼠血清高迁徙率族蛋白-1水平的时相变化及意义[J]. 华中科技大学学报(医学版)， 2004， 33(4)： 466-468.
14. 张喜平，林谦. 急性胰腺炎时炎症介质与细胞凋亡关系的研究进展[J]. 世界华人消化杂志， 2005， 13(23)： 2773-2777.
15. Wang H， Yang H， Tracey KJ. Extracellular role of HMGB1 in inflammation and sepsis[J]. J Intern Med， 2004， 255(3)： 320-331.

CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY

Research of TAP Induction The Pancreatic Acinar Cells to Release HMGB1 in Rat

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