Effects of Estrogen Receptor Subtypes on Biological Behaviors of Human Breast Cancer Cell Line MCF-7_CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY

Authors：

NIU Xiulong ¹ , WANG Yifei ² , SUN Yi ¹ , WANG Yue ^1,3

1.Department of Immunology, Medical College of Chinese People’s Armed Police Forces， Tianjin 300162, China;;
2.Editorial Office of Acta Academiae Medicinae, Medical College of Chinese People’s Armed Police Forces， Tianjin 300162， China;;
3.Tianjin Key Laboratory for Biomarkers of Occupational and Environmental hazard, Medical College of Chinese People’s Armed Police Forces， Tianjin 300162, China;

Corresponding author：

Keywords：

Estrogen receptor; RNA interference; Breast cancer

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ObjectiveTo establish MCF-7 cell lines with different ERα/ERβ expression level and observe their biological behaviors. MethodsERα or ERβ gene were silenced by RNA interference, and the cell lines with different ERα or ERβ expression level were obtained in MCF cell lines. MTT assay, flow cytometry, RT-PCR, double layer softagar clony formation test, and Matrigel adhesion assay were used to detect the abilities of cell proliferation, apoptosis, tumorigenesis, and adhesion. ResultsThe stable expression cell lines with ERαlow/ERβhigh or ERαhigh/ERβlow were established successfully. After ERα gene knockdown, MCF-7 grew slowly and was arrested at phase G0-G1. Apoptosis of MCF-7 was induced and the capacity of tumorigenesis and adhesion in vitro were weakened. However, the characteristics mentioned above except for adhesion changed to the opposite sides after ERβ gene knockdown. ConclusionsThe ERα gene silence can inhibit the formation of tumor, however the ERβ gene silence can promote tumor growth, invasion, and metastasis. Therefore, may be a useful approach on the breast cancer therapy.

Citation： NIU Xiulong ,WANG Yifei,SUN Yi,WANG Yue. Effects of Estrogen Receptor Subtypes on Biological Behaviors of Human Breast Cancer Cell Line MCF-7. CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY, 2011, 18(7): 711-716. doi: Copy

1.	Nangaku M. Chronic hypoxia and tubulointerstitial injury: a final common pathway to endstage renal failure [J]. J Am Soc Nephrol, 2006, 17(1): 1725.
2.	Welboren WJ, Sweep FC, Span PN, et al. Genomic actions of estrogen receptor alpha: what are the targets and how are they regulated? [J]. Endocr Relat Cancer, 2009, 16(4): 10731089.
3.	Paech K, Webb P, Kuiper GG, et al. Differential ligand activation of estrogen receptors ERalpha and ERbeta at AP1 sites [J]. Science, 1997, 277(5331): 15081510.
4.	Hayashi SI, Eguchi H, Tanimoto K, et al. The expression and function of estrogen receptor alpha and beta in human breast cancer and its clinical application [J]. Endocr Relat Cancer, 2003, 10(2): 193202.
5.	Meacham WD, Antoon JW, Burow ME, et al. Sphingolipids as determinants of apoptosis and chemoresistance in the MCF7 cell model system [J]. Exp Biol Med (Maywood), 2009， 234(11): 1253 1263.
6.	Speirs V. Oestrogen receptor in breast cancer: good, bad or still too early to tell [J]. J Pathol, 2002, 197(2)： 143147.
7.	Zhao C, DahlmanWright K, Gustafsson JA. Estrogen signaling via estrogen receptor beta [J]. J Biol Chem, 2010, 285(51): 3957539579.
8.	Drummond AE, Fuller PJ. The importance of ERbeta signalling in the ovary [J]. J Endocrinol, 2010, 205(1): 1523.
9.	Roger P, Sahla ME, Makela S, et al. Decreased expression of estrogen receptor beta protein in proliferative preinvasive mammary tumors [J]. Cancer Res, 2001, 61(6): 25372541.
10.	Paruthiyil S, Parmar H, Kerekatte V, et al. Estrogen receptor beta inhibits human breast cancer cell proliferation and tumor formation by causing a G2 cell cycle arrest [J]. Cancer Res, 2004, 64(1): 423428.
11.	Strom A, Hartman J, Foster JS, et al. Estrogen receptor beta inhibits 17 betaestradiolstimulated proliferation of the breast cancer cell line T47D [J]. Proc Natl Acad Sci USA, 2004, 101(6): 15661571.
12.	Helguero LA, Faulds MH, Gustafsson JA, et al. Estrogen receptors alfa (ERalpha) and beta (ERbeta) differentially regulate proliferation and apoptosis of the normal murine mammary epithelial cell line HC11 [J]. Oncogene, 2005, 24(44): 66056616.
13.	Chen L, Qiu J, Yang C, et al. Identification of a novel estrogen receptor beta1 binding partner, inhibitor of differentiation1, and role of ERbeta1 in human breast cancer cells [J]. Cancer Lett, 2009, 278(2): 210219．.
14.	Grober OM, Mutarelli M, Giurato G, et al. Global analysis of estrogen receptor beta binding to breast cancer cell genome reveals an extensive interplay with estrogen receptor alpha for target gene regulation [J]. BMC Genomics, 2011, 14(12): 3652.
15.	Tonetti DA, Rubenstein R, DeLeon M, et al. Stable transfection of an estrogen receptor beta cDNA isoform into MDAMB231 breast cancer cells [J]. J Steroid Biochem Mol Biol, 2003, 87(1): 4755.
16.	Hou YF, Yuan ST, Li HC, et al. ERbeta exerts multiple stimulative effects on human breast carcinoma cells [J]. Oncogene, 2004, 23(34): 57995806.
17.	侯意枫，袁胜涛，李鹤成，等. 雌激素受体β对人乳腺癌细胞株生物学特性的影响 [J]. 中华肿瘤杂志， 2005, 27(7): 389392.
18.	于冰，孙治君. 凋亡相关基因bcl2、bax、bad与乳腺癌 [J]. 中国普外基础与临床杂志, 2007, 14(6): 739742.
19.	吴敏，吴静，徐玥，等. 小RNA干扰PDK1抑制乳腺肿瘤细胞侵袭 [J]. 中国肿瘤临床, 2009, 36(12): 697700.
20.	董林，何晓东，陶绍能，等. 甲氨蝶呤对映体诱导的肺癌A549耐药细胞株中VEGF及其受体表达差异的研究 [J]. 肿瘤, 2009, 29(5): 404408.
21.	Myler HA, West JL. Heparanase and platelet factor4 induce smooth muscle cell proliferation and migration via bFGF release from the ECM [J]. J Biochem, 2002, 131(6): 913922.
22.	高德宗，高华，郭秀会，等. 他莫昔芬与γ干扰素联合抗人乳腺癌细胞株的作用及其机制研究 [J]. 中国普通外科杂志， 2007， 16(11)： 10761080.
23.	高德宗，孙靖中，高华，等. γ干扰素增强他莫昔芬抗乳腺癌作用的体外研究 [J]. 中国肿瘤生物治疗杂志， 2006， 13(1)： 4549.
24.	Pedicini M, Barrens F, Clancy T, et al. Combining network modeling and gene expression microarray analysis to explore the dynamics of Th1 and Th2 cell regulation [J]. PLoS Comput Biol, 2010, 6(12): e1001032.
25.	孙桂明，李新焕，王宝中，等. 维甲酸联合他莫昔芬对人乳腺癌细胞株作用的研究 [J]. 中华肿瘤防治杂志， 2010， 17(18)： 14251428.

1. Nangaku M. Chronic hypoxia and tubulointerstitial injury: a final common pathway to endstage renal failure [J]. J Am Soc Nephrol, 2006, 17(1): 1725.
2. Welboren WJ, Sweep FC, Span PN, et al. Genomic actions of estrogen receptor alpha: what are the targets and how are they regulated? [J]. Endocr Relat Cancer, 2009, 16(4): 10731089.
3. Paech K, Webb P, Kuiper GG, et al. Differential ligand activation of estrogen receptors ERalpha and ERbeta at AP1 sites [J]. Science, 1997, 277(5331): 15081510.
4. Hayashi SI, Eguchi H, Tanimoto K, et al. The expression and function of estrogen receptor alpha and beta in human breast cancer and its clinical application [J]. Endocr Relat Cancer, 2003, 10(2): 193202.
5. Meacham WD, Antoon JW, Burow ME, et al. Sphingolipids as determinants of apoptosis and chemoresistance in the MCF7 cell model system [J]. Exp Biol Med (Maywood), 2009， 234(11): 1253 1263.
6. Speirs V. Oestrogen receptor in breast cancer: good, bad or still too early to tell [J]. J Pathol, 2002, 197(2)： 143147.
7. Zhao C, DahlmanWright K, Gustafsson JA. Estrogen signaling via estrogen receptor beta [J]. J Biol Chem, 2010, 285(51): 3957539579.
8. Drummond AE, Fuller PJ. The importance of ERbeta signalling in the ovary [J]. J Endocrinol, 2010, 205(1): 1523.
9. Roger P, Sahla ME, Makela S, et al. Decreased expression of estrogen receptor beta protein in proliferative preinvasive mammary tumors [J]. Cancer Res, 2001, 61(6): 25372541.
10. Paruthiyil S, Parmar H, Kerekatte V, et al. Estrogen receptor beta inhibits human breast cancer cell proliferation and tumor formation by causing a G2 cell cycle arrest [J]. Cancer Res, 2004, 64(1): 423428.
11. Strom A, Hartman J, Foster JS, et al. Estrogen receptor beta inhibits 17 betaestradiolstimulated proliferation of the breast cancer cell line T47D [J]. Proc Natl Acad Sci USA, 2004, 101(6): 15661571.
12. Helguero LA, Faulds MH, Gustafsson JA, et al. Estrogen receptors alfa (ERalpha) and beta (ERbeta) differentially regulate proliferation and apoptosis of the normal murine mammary epithelial cell line HC11 [J]. Oncogene, 2005, 24(44): 66056616.
13. Chen L, Qiu J, Yang C, et al. Identification of a novel estrogen receptor beta1 binding partner, inhibitor of differentiation1, and role of ERbeta1 in human breast cancer cells [J]. Cancer Lett, 2009, 278(2): 210219．.
14. Grober OM, Mutarelli M, Giurato G, et al. Global analysis of estrogen receptor beta binding to breast cancer cell genome reveals an extensive interplay with estrogen receptor alpha for target gene regulation [J]. BMC Genomics, 2011, 14(12): 3652.
15. Tonetti DA, Rubenstein R, DeLeon M, et al. Stable transfection of an estrogen receptor beta cDNA isoform into MDAMB231 breast cancer cells [J]. J Steroid Biochem Mol Biol, 2003, 87(1): 4755.
16. Hou YF, Yuan ST, Li HC, et al. ERbeta exerts multiple stimulative effects on human breast carcinoma cells [J]. Oncogene, 2004, 23(34): 57995806.
17. 侯意枫，袁胜涛，李鹤成，等. 雌激素受体β对人乳腺癌细胞株生物学特性的影响 [J]. 中华肿瘤杂志， 2005, 27(7): 389392.
18. 于冰，孙治君. 凋亡相关基因bcl2、bax、bad与乳腺癌 [J]. 中国普外基础与临床杂志, 2007, 14(6): 739742.
19. 吴敏，吴静，徐玥，等. 小RNA干扰PDK1抑制乳腺肿瘤细胞侵袭 [J]. 中国肿瘤临床, 2009, 36(12): 697700.
20. 董林，何晓东，陶绍能，等. 甲氨蝶呤对映体诱导的肺癌A549耐药细胞株中VEGF及其受体表达差异的研究 [J]. 肿瘤, 2009, 29(5): 404408.
21. Myler HA, West JL. Heparanase and platelet factor4 induce smooth muscle cell proliferation and migration via bFGF release from the ECM [J]. J Biochem, 2002, 131(6): 913922.
22. 高德宗，高华，郭秀会，等. 他莫昔芬与γ干扰素联合抗人乳腺癌细胞株的作用及其机制研究 [J]. 中国普通外科杂志， 2007， 16(11)： 10761080.
23. 高德宗，孙靖中，高华，等. γ干扰素增强他莫昔芬抗乳腺癌作用的体外研究 [J]. 中国肿瘤生物治疗杂志， 2006， 13(1)： 4549.
24. Pedicini M, Barrens F, Clancy T, et al. Combining network modeling and gene expression microarray analysis to explore the dynamics of Th1 and Th2 cell regulation [J]. PLoS Comput Biol, 2010, 6(12): e1001032.
25. 孙桂明，李新焕，王宝中，等. 维甲酸联合他莫昔芬对人乳腺癌细胞株作用的研究 [J]. 中华肿瘤防治杂志， 2010， 17(18)： 14251428.

CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY

Effects of Estrogen Receptor Subtypes on Biological Behaviors of Human Breast Cancer Cell Line MCF-7

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