The effects of interleukin 17 on the proliferation, migration and apoptosis of human retinal vascular endothelial cells_Chinese Journal of Ocular Fundus Diseases

Authors：

XuJing , LiuGaoqin , ChenZhigang ,  陆培荣

Department of Ophthalmology, The First Affiliated Hospital of Soochow University, Suzhou 215006, China;

Corresponding author：

陆培荣, Email: lupeirong@suda.edu.cn

Keywords：

Interleukin-17; Endothelial cells/pathophysiology; Cell proliferation; Cell movement; Apoptosis

DOI：

10.3760/cma.j.issn.1005-1015.2015.01.017

Video：

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Objective To address the effect and mechanism of interleukin 17 (IL-17) on the proliferation, migration and apoptosis of human retinal vascular endothelial cells (HREC). Methods IL-17 receptor (IL-17R) mRNA and protein expression in human retinal vascular endothelial cells (HREC) were quantified by reverse transcription polymerase chain reaction (RT-PCR) and Western blot. Cell proliferation of HREC was examined using CCK-8 assay in the presence of different concentrations of IL-17. Cell migration of HREC was detected using wound scratch assay. Flow cytometry was used to test the effect of IL-17 on the apoptosis of HREC. The effects of IL-17 on HREC expression of basic fibroblast growth factor (bFGF), Caspase-3 and thrombospondin-1 (TSP-1) were quantified by reverse transcriptase polymerase chain reaction (RT-PCR). The effect of IL-17 on HREC expression of Caspase-3 was examined using Western blot. Results IL-17 receptor (IL-17R) expressed in HREC as quantified by RT-PCR and Western blot. The proliferation of HREC in the presence of IL-17 was promoted in a dosage-dependent manner (t=-3.235, -6.276;P=0.032, 0.000). Wound scratch assay showed a significant increase in the migrated distance of HREC with IL-17 stimulation under the concentration of 100μg/L(t=-3.551, -2.849; P=0.006, 0.019), 200μg/L(t=-10.347, -4.519; P=0.000, 0.001) and 500μg/L (t=-3.541, -2.607; P=0.008, 0.036). The intervention of 200μg/L IL-17 can effectively inhibit the apoptosis of HREC, compared with the control group using flow cytometry (t=5.682, P=0.047). RT-PCR results showed that IL-17 can promote the expression of bFGF and inhibit the expression of Caspase-3 and TSP-1. Western blot result also showed that IL-17 can suppress the protein expression of Caspase-3. Conclusion The mechanism of IL-17 promoting proliferation, migration but suppress apoptosis of HREC may via regulating the expression of bFGF and Caspase-3.

Citation： XuJing, LiuGaoqin, ChenZhigang. The effects of interleukin 17 on the proliferation, migration and apoptosis of human retinal vascular endothelial cells. Chinese Journal of Ocular Fundus Diseases, 2015, 31(1): 67-71. doi: 10.3760/cma.j.issn.1005-1015.2015.01.017 Copy

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1. Singh RP, Hasan S, Sharma S, et al. Th17 cells in inflammation and autoimmunity[J]. Autoimmun Rev, 2014, 13(12):1174-1181.
2. Yang B, Kang H, Fung A, et al. The role of interleukin 17 in tumour proliferation, angiogenesis, and metastasis. Mediators Inflamm, 2014, 2014:623759.http://www.hindawi.com/journals/mi/2014/623759/.
3. Chen Y, Zhong M, Liang L, et al. Interleukin-17 induces angiogenesis in human choroidal endothelial cells in vitro[J]. Invest Ophthalmol Vis Sci, 2014, 55(10):6968-6975.
4. Chen Z, Liu G, Lu P, et al. Adrenomedullin22-52 suppresses high-glucose-induced migration, proliferation, and tube formation of human retinal endothelial cells[J]. Mol Vis, 2014, 20:259-269.
5. 肖艳辉, 刘高勤, 陈志刚, 等.重组干扰素诱导蛋白-10对视网膜血管内皮细胞增生、迁移及管腔形成的影响[J].中华眼底病杂志, 2013, 29(1):5-8.
6. Cheng R, Li C, Wei L, et al. The artemisinin derivative artesunate inhibits corneal neovascularization by inducing ROS-dependent apoptosisin vascular endothelial cells[J]. Invest Ophthalmol Vis Sci, 2013, 54(5):3400-3409.
7. 赫雪飞, 刘高勤, 陈志刚, 等. CCR3拮抗剂对视网膜血管内皮细胞管腔形成的影响及机制[J].眼科新进展, 2013, 33(12):1106-1109, 1114.
8. Numasaki M, Fukushi J, Ono M, et al. Interleukin-17 promotes angiogenesis and tumor growth[J]. Blood, 2003, 101(7):2620-2627.
9. Chauhan SK, Jin Y, Goyal S, et al. A novel pro-lymphangiogenic function forTh17/IL-17[J]. Blood, 2011, 118(17):4630-4634.
10. Moran EM, Connolly M, Gao W, et al. Interleukin-17A induction of angiogenesis, cell migration, and cytoskeletal rearrangement[J]. Arthritis Rheum, 2011, 63(11):3263-3273.
11. Wang W, Fan YQ, Lv Z, et al. Interleukin-25 promotes basic fibroblast growth factor expression by human endothelial cells through interaction with IL-17RB, but not IL-17RA[J]. Clin Exp Allergy, 2012, 42(11):1604-1614.
12. Javerzat S, Auguste P, Bikfalvi A. The role of fibroblast growth factors in vascular development[J]. Trends Mol Med, 2002, 8(10):483-489.
13. Armstrong LC, Bornstein P. Thrombospondins 1 and 2 function as inhibitors of angiogenesis[J]. Matrix Biol, 2003, 22(1):63-71.

Chinese Journal of Ocular Fundus Diseases

The effects of interleukin 17 on the proliferation, migration and apoptosis of human retinal vascular endothelial cells

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