Changes in open probability and protein expression of large conductance Ca<sup>2+</sup>-activated K<sup>+</sup> channel in retinal vascular smooth muscle cells of diabetic rats_Chinese Journal of Ocular Fundus Diseases

Authors：

CaoJia , SunWei , ShaoJun , 谢田华 , 殷丽 , 吴莹 , 王如兴 ,  姚勇

Department of Ophthalmology, Wuxi People's Hospital Affiliated to Nanjing Medical University, Wuxi 214023, China;

Corresponding author：

姚勇, Email: pardl@126.com

Keywords：

Diabetic retinopathy/etiology; Retinal artery; Potassium channels; Patch-clamp techniques; Animal experimentation

DOI：

10.3760/cma.j.issn.1005-1015.2015.02.013

Video：

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Abstract Full text Figures/Tables Video References Cited by

Objective To observe the changes in open probability and protein expression of large conductance Ca²⁺-activated K⁺ (BK) channel in retinal vascular smooth muscle cells (RVSMCs) of diabetic rats. Methods Streptozotocin (STZ)-induced rat diabetic animal model was established by STZ injection intraperitoneally.RVSMCs were isolated by enzyme digestion. The BK currents in control and diabetic groups were recorded by patch clamp technique in single channel configuration. BK channel protein expression in control and diabetic group were measured by Western blot. Results Compared with control group, the NP₀ of BK channels in diabetic group were significantly increased (t=4.260, P < 0.05). Compared with control group, there was no significant difference inα-subunit protein expression in diabetic group in RVSMCs (t=10.126, P > 0.05); however, β1-subunit protein expression was remarkably increased in diabetic group (t=5.146, P < 0.05). Conclusion The NP₀ of BK channels andβ1-subunit protein expression are increased in RVSMCs of diabetic rats.

Citation： CaoJia, SunWei, ShaoJun. Changes in open probability and protein expression of large conductance Ca²⁺-activated K⁺ channel in retinal vascular smooth muscle cells of diabetic rats. Chinese Journal of Ocular Fundus Diseases, 2015, 31(2): 162-164. doi: 10.3760/cma.j.issn.1005-1015.2015.02.013 Copy

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12.	孙尉, 王如新.大电导钙激活钾离子通道与糖尿病视网膜动脉病变[J].中华糖尿病杂志, 2012, 4(3):186-188.
13.	钱玲玲, 刘晓宇, 王如兴.大电导钙离子激活钾通道与糖尿病血管病变的相关性探讨[J].中华老年医学杂志, 2014, 33(5):569-572.
14.	汤徐, 钱玲玲, 王如兴, 等.大电导钙离子激活钾通道在心血管疾病中的研究进展[J].中华老年多器官疾病杂志, 2014, 13(1):76-80.
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1. Li Y, Liao Y, Fan A, et al. Asian American/Pacific Islander paradox in diabetic retinopathy:findings from the behavioral risk factor surveillance system, 2006-2008[J]. Ethn Dis, 2010, 20(2):111-117.
2. McVicar CM, Hamilton R, Colhoun LM, et al. Intervention with an erythropoietin-derived peptide protects against neuroglial and vascular degeneration during diabetic retinopathy[J]. Diabetes, 2011, 60(11):2995-3005.
3. Bek T. Lack of correlation between short-term dynamics of diabetic retinopathy lesions and the arterial blood pressure[J]. Graefe's Arch Clin Exp Ophthalmol, 2011, 249(2):267-271.
4. Yang WY. Achieve great success, and blaze a trail:review of clinical and basic research progress of Chinese diabetes in the 21st century[J]. Chin Med J, 2009, 122(21):2525-2529.
5. Yamagishi S, Ueda S, Matsui T, et al. Role of advanced glycation end products (AGEs) and oxidative stress in diabetic retinopathy[J]. Curr Pharm Des, 2008, 14(10):962-968.
6. 王如兴, 李肖蓉, 羊镇宇, 等.大电导钙离子激活钾通道对糖尿病大鼠冠状动脉血管张力的调节[J].中华医学杂志, 2010, 90(36):2575-2578.
7. Wang R, Jiang W. Changes of action potential and L-type calcium channel current of Sprague-Dawley rat ventricular myocytes by different amlodipine isomers[J]. Can J Physiol Pharmacol, 2008, 86(9):620-625.
8. Saleh SN, Albert AP, Peppiatt-Wildman CM, et al. Diverse properties of store-operated TRPC channels activated by protein kinase C in vascular myocytes[J]. J Physiol, 2008, 586(10):2463-2476.
9. Ghatta S, Nimmagadda D, Xu X, et al. Large-conductance, calcium-activated potassium channels:structural and functional implications[J]. Pharmacol Ther, 2006, 110(1):103-116.
10. Salkoff L, Butler A, Ferreira G, et al. High-conductance potassium channels of the SLO family[J]. Nat Rev Neurosci, 2006, 7(12):921-931.
11. Hou S, Heinemann SH, Hoshi T. Modulation of BKCa channel gating by endogenous signaling molecules[J]. Physiology, 2009, 24(1):26-35.
12. 孙尉, 王如新.大电导钙激活钾离子通道与糖尿病视网膜动脉病变[J].中华糖尿病杂志, 2012, 4(3):186-188.
13. 钱玲玲, 刘晓宇, 王如兴.大电导钙离子激活钾通道与糖尿病血管病变的相关性探讨[J].中华老年医学杂志, 2014, 33(5):569-572.
14. 汤徐, 钱玲玲, 王如兴, 等.大电导钙离子激活钾通道在心血管疾病中的研究进展[J].中华老年多器官疾病杂志, 2014, 13(1):76-80.
15. McGahon MK, Dawicki JM, Scholfield CN, et al. A-type potassium current in retinal arteriolar smooth muscle cells[J]. Invest Ophthalmol Vis Sci, 2005, 46(9):3281-3287.

Chinese Journal of Ocular Fundus Diseases

Changes in open probability and protein expression of large conductance Ca²⁺-activated K⁺ channel in retinal vascular smooth muscle cells of diabetic rats

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Chinese Journal of Ocular Fundus Diseases

Changes in open probability and protein expression of large conductance Ca2+-activated K+ channel in retinal vascular smooth muscle cells of diabetic rats

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Changes in open probability and protein expression of large conductance Ca²⁺-activated K⁺ channel in retinal vascular smooth muscle cells of diabetic rats