The effects of bulbar subconjunctival and periocular injection of dexamethasonone on blood glucose levels of type 1 diabetic mellitus rats_Chinese Journal of Ocular Fundus Diseases

Authors：

WangBin ,  ZhouQiong

Department of Ophthalmology, The First Affiliated Hospital of Nanchang University, Nanchang 330006, China;

Corresponding author：

ZhouQiong, Email: qiongz-ms@126.com

Keywords：

Dexamethasone/adverse effects; Blood glucose; Injections; Diabetes mellitus, experimental

DOI：

10.3760/cma.j.issn.1005-1015.2015.02.014

Video：

Export PDF Favorites Scan Get Citation

Abstract Full text Figures/Tables Video References Cited by

Objective To observe the effects of bulbar subconjunctival and periocular injection of dexamethasonone on blood glucose levels of type 1 diabetic mellitus (T1DM)rats. Methods 80 healthy adult male Sprague-Dawley rats were randomly divided into GroupⅠ(n=40) and GroupⅡ(n=40). GroupⅠrats received intraperitoneal (IP) injection of streptozotocin to induce T1DM model, while GroupⅡrats received IP injection of citrate buffer solution and was the control group.GroupⅠrats and GroupⅡrats were further divided into four subgroups:A (n=10), a (n=10), B (n=10), and b (n=10). Subgroup-A rats received bulbar subconjunctival injection of dexamethasone, subgroup-a rats received bulbar subconjunctival injection of saline, subgroup-B rats received periocular injection of dexamethasone, subgroup-b rats received periocular injection of saline. After the injection, rats were fasted but could drink water. Tail vein blood samples were collected and the blood glucose level was measured by glucose monitor. Results After modeling, the blood glucose level of GroupⅠand GroupⅡrats was(9.31±1.79) mmol/L and (5.72±0.80) mmol/L respectively, the difference was statistically significant (P < 0.05). The blood glucose level of GroupⅠrats reached the peak in 3h after injection. In 6-24 h after injection, the blood glucose level of GroupⅠA rats was obviously increased than that of the blood glucose level of Group Ia rats and the difference was statistically significant (P < 0.05). In 3-24 hours after injection, the blood glucose level of GroupⅠB rats was obviously increased than that of the blood glucose level of GroupⅠb rats and the difference was statistically significant (P < 0.05). Comparing the blood glucose level during different injection time between GroupⅠA rats and GroupⅠB rats, between GroupⅠa rats and GroupⅠb rats, the difference was not statistically significant (P > 0.05). In 3-24 hours after injection, the blood glucose level of GroupⅡA rats was obviously increased than that of the blood glucose level of GroupⅡa rats and the difference was statistically significant (P < 0.05); the blood glucose level of GroupⅡB rats was obviously increased than that of the blood glucose level of GroupⅡb rats and the difference was statistically significant (P < 0.05). Comparing the blood glucose level during different injection time between GroupⅡA rats and GroupⅡB rats, between GroupⅡa rats and GroupⅡb rats, the difference was not statistically significant (P > 0.05). Conclusion Bulbar subconjunctival injection and periocular injection of dexamethasone could both increase the blood glucose of TIDM rats, but these two injection methods had no differences on the blood glucose level.

Citation： WangBin, ZhouQiong. The effects of bulbar subconjunctival and periocular injection of dexamethasonone on blood glucose levels of type 1 diabetic mellitus rats. Chinese Journal of Ocular Fundus Diseases, 2015, 31(2): 165-168. doi: 10.3760/cma.j.issn.1005-1015.2015.02.014 Copy

1.	Feldman-Billard S, Du Pasquier-Fediaevsky L, Héron E. Hyperglycemia after repeated periocular dexamethasone injections in patients with diabetes[J].Ophthalmology, 2006, 113(10):1720-1723.
2.	Asensio-Sánchez VM, Soto A, Conde S, et al.Retrobulbar triamcinolone injection and glycemic control[J].Arch Soc Esp Oftalmol, 2009, 84(12):599-603.
3.	张巨彪, 苏秀兰, 欧阳晓晖. 1型糖尿病大鼠模型的建立及观察[J].医学综述, 2013, 19(2):335-337.
4.	Matteucci E, Giampietro O. Proposal open for discussion:defining agreed diagnostic procedures in experimental diabetes research[J]. J Ethnopharmacol, 2008, 115(2):163-172.
5.	张阔, 田枫, 康爱君, 等.戊巴比妥钠, 水合氯醛, 氨基甲酸乙酯麻醉对雄性SD大鼠血液学指标的影响[J].中国比较医学杂志, 2007, 17(8):496-496.
6.	Weijtens O, Schoemaker RC, Romijn FP, et al. Intraocular penetration and systemic absorption after topical application of dexamethasone disodium phosphate[J]. Ophthalmology, 2002, 109(10):1887-1891.
7.	王竹, 杨月欣, 向雪松, 等.实验大鼠血糖正常范围的估算[J].卫生研究, 2010, 39(2):133-137.
8.	Molina PE. Neurobiology of the stress response:contribution of the sympathetic nervous system to the neuroimmune axis in traumatic injury[J]. Shock, 2005, 24(1):3-10.
9.	Shaw JH, Wolfe RR. An integrated analysis of glucose, fat, and protein metabolism in severely traumatized patients:studies in the basal state and the response to total parenteral nutrition[J]. Annals of surgery, 1989, 209(1):63-72.
10.	Jeremitsky E, Omert LA, Dunham CM, et al. The impact of hyperglycemia on patients with severe brain injury[J]. J Trauma, 2005, 58(1):47-50.
11.	Friday E, Ledet J, Turturro F. Response to dexamethasone is glucose-sensitive in multiple myeloma cell lines[J]. J Exp Clin Cancer Res, 2011, 30(1):1-7.
12.	Abdelli S, Bonny C. JNK3 maintains expression of the insulin receptor substrate 2 (IRS2) in insulin-secreting cells:functional consequences for insulin signaling. PloS one, 2012, 7(5):35997.http://dx.plos.org/10.1371/journal.pone.0035997..
13.	Nesher R, Cerasi E. Modeling phasic insulin release immediate and time-dependent effects of glucose[J]. Diabetes, 2002, 51(Suppl 1):S53-59.
14.	Weijtens O, Feron EJ, Schoemaker RC, et al. High concentration of dexamethasone in aqueous and vitreous after subconjunctival injection[J]. Am J Ophthalmol, 1999, 128(2):192-197.

1. Feldman-Billard S, Du Pasquier-Fediaevsky L, Héron E. Hyperglycemia after repeated periocular dexamethasone injections in patients with diabetes[J].Ophthalmology, 2006, 113(10):1720-1723.
2. Asensio-Sánchez VM, Soto A, Conde S, et al.Retrobulbar triamcinolone injection and glycemic control[J].Arch Soc Esp Oftalmol, 2009, 84(12):599-603.
3. 张巨彪, 苏秀兰, 欧阳晓晖. 1型糖尿病大鼠模型的建立及观察[J].医学综述, 2013, 19(2):335-337.
4. Matteucci E, Giampietro O. Proposal open for discussion:defining agreed diagnostic procedures in experimental diabetes research[J]. J Ethnopharmacol, 2008, 115(2):163-172.
5. 张阔, 田枫, 康爱君, 等.戊巴比妥钠, 水合氯醛, 氨基甲酸乙酯麻醉对雄性SD大鼠血液学指标的影响[J].中国比较医学杂志, 2007, 17(8):496-496.
6. Weijtens O, Schoemaker RC, Romijn FP, et al. Intraocular penetration and systemic absorption after topical application of dexamethasone disodium phosphate[J]. Ophthalmology, 2002, 109(10):1887-1891.
7. 王竹, 杨月欣, 向雪松, 等.实验大鼠血糖正常范围的估算[J].卫生研究, 2010, 39(2):133-137.
8. Molina PE. Neurobiology of the stress response:contribution of the sympathetic nervous system to the neuroimmune axis in traumatic injury[J]. Shock, 2005, 24(1):3-10.
9. Shaw JH, Wolfe RR. An integrated analysis of glucose, fat, and protein metabolism in severely traumatized patients:studies in the basal state and the response to total parenteral nutrition[J]. Annals of surgery, 1989, 209(1):63-72.
10. Jeremitsky E, Omert LA, Dunham CM, et al. The impact of hyperglycemia on patients with severe brain injury[J]. J Trauma, 2005, 58(1):47-50.
11. Friday E, Ledet J, Turturro F. Response to dexamethasone is glucose-sensitive in multiple myeloma cell lines[J]. J Exp Clin Cancer Res, 2011, 30(1):1-7.
12. Abdelli S, Bonny C. JNK3 maintains expression of the insulin receptor substrate 2 (IRS2) in insulin-secreting cells:functional consequences for insulin signaling. PloS one, 2012, 7(5):35997.http://dx.plos.org/10.1371/journal.pone.0035997..
13. Nesher R, Cerasi E. Modeling phasic insulin release immediate and time-dependent effects of glucose[J]. Diabetes, 2002, 51(Suppl 1):S53-59.
14. Weijtens O, Feron EJ, Schoemaker RC, et al. High concentration of dexamethasone in aqueous and vitreous after subconjunctival injection[J]. Am J Ophthalmol, 1999, 128(2):192-197.

Chinese Journal of Ocular Fundus Diseases

The effects of bulbar subconjunctival and periocular injection of dexamethasonone on blood glucose levels of type 1 diabetic mellitus rats

Abstract Full text Figures/Tables Video References Cited by

Previous Article

Next Article

Format

Content