Expression of apoptosis-associated proteins in the rat retina of ocular ischemic syndrome_Chinese Journal of Ocular Fundus Diseases

Authors：

XieGuoli , FuQiang , ZhaoLu ,  王艳玲

Department of Ophthalmology, Beijing Friendship Hospital, Capital Medical University, Beijing 100050, China;

Corresponding author：

王艳玲, Email: wangyanl@ccmu.edu.cn

Keywords：

Ischemia/physiopathology; Apoptosis; Caspase; bcl-2-associated X protein; Animal experimentation

DOI：

10.3760/cma.j.issn.1005-1015.2015.06.015

Video：

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Objective To observe the expression of Caspase-3, Caspase-8, Bcl-2, Bax in the rat retina of ocular ischemic syndrome (OIS). Methods 30 Brown Norway rats were randomly divided into experimental group and control group, 15 rats in each group. The rats in experimental group were established a model through ligating the bilateral common carotid artery. At 3 months after modeling, the retinal thickness and ganglion cell (RGC) density were measured by hematoxylin eosin staining; the expression of Caspase 3, Caspase 8, Bax and Bcl-2 in the retina was measured by quantitative real-time reverse transcription polymerase chain reaction. Results The RGC density in control group and experimental group was 61.97±9.07 and 38.1±5.98, respectively. Compared to the control group, the RGC density was diminished in the experimental group (t=3.059, P < 0.01). A significant decrease was found in the total retina (t=3.036), inner plexiform (t=3.715), inner nuclear (t=3.339) and outer plexiform (t=3.341) thickness (P < 0.05). However, no change of the thickness was evident in the outer nuclear layers (t=2.000, P > 0.05). The levels of protein and RNA expression of Caspase 3, Caspase 8 and Bax in the retina were increased in experimental group (F=17.036, 7.787, 11.431, 11.162, 17.763, 12.183; P < 0.05), while the Bcl-2 expression were decreased (F=10.298, 12.047; P < 0.05). Conclusions There is obvious expression of apoptosis-associated proteins in the rat retina of OIS. Caspase 3, Caspase 8 and Bax expression are increased, while Bcl-2 expression are decreased.

Citation： XieGuoli, FuQiang, ZhaoLu. Expression of apoptosis-associated proteins in the rat retina of ocular ischemic syndrome. Chinese Journal of Ocular Fundus Diseases, 2015, 31(6): 571-574. doi: 10.3760/cma.j.issn.1005-1015.2015.06.015 Copy

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1. Yamamoto H, Schmidt-Kastner R, Hamasaki DI, et al. Complex neurodegeneration in retina following moderate ischemia induced by bilateral common carotid artery occlusion in Wistar rats[J].Exp Eye Res, 2006, 82(5):767-779.
2. Lavinsky D, Arterni NS, Achaval M, et al. Chronic bilateral common carotid arterv occlusion:a model for ocular ischemic syndrome in the rat[J]. Graefe's Arch Clin Exp Ophthalmol, 2006, 244(2):199-204.
3. Stevens WD, Fortin T, Pappas BA. Retinal and optic nerve degeneration after chronic carotid ligation: time course and role of light exposure[J]. Stroke, 2002, 33(4):1107-1112.
4. Concha NO, Abdel-Meguid SS. Controlling apoptosis by inhibition of caspases[J]. Curr Med Chem, 2002, 9(6):713-726.
5. De Maria R, Lentil L, Malisan F, et al. Requirement for GD3 ganglioside in CD95 and ceramide-induced apoptosis[J].Science, 1997, 277(5332):1652-1655.
6. Loor G, Kondapalli J, Iwase H, et al.Mitochondrial oxidant stress triggers cell death in simulated ischemia reperfusion[J].Biochim Biophys Acta, 2011, 1813(7):1382-1394.

Chinese Journal of Ocular Fundus Diseases

Expression of apoptosis-associated proteins in the rat retina of ocular ischemic syndrome

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