Prediction and bioinformatic analysis of hsa-miRNA-451 target genes_Chinese Journal of Ocular Fundus Diseases

Authors：

ChenZhenna , ShaoYan ,  LiXiaorong

Tianjin Medical University Hospital, Tianjin Medical University Eye Institute, College of Optometry and Ophthalmology, Tianjin Medical University, Tianjin 300384, China;

Corresponding author：

LiXiaorong, Email: xiaorli@163.com

Keywords：

Gene therapy; Forecasting; Computational biology; Diabetic retinopathy/physiopathology

DOI：

10.3760/cma.j.issn.1005-1015.2015.06.022

Video：

Export PDF Favorites Scan Get Citation

Abstract Full text Figures/Tables Video References Cited by

Objective To predict as well as bioinformatically analyze the target genes of has-miR-451. Methods miRBase, miRanda, TargetScan and PicTar were used to predict the target genes of hsa-miRNA-451. The functions of the target genes were demonstrated by Gene Ontology and pathway enrichment analysis. P < 0.05 was set as statistically significant. Results 18 target spots of hsa-miRNA-451 were predicted by 3 databases or prediction software at least. The functions of the target genes were enriched in proliferation and development of epithelial cells and regulation of kinase activity (P < 0.05). Pathway analysis showed that transforming growth factor-beta signaling pathway, mitogen-activated protein kinase signaling pathway, epidermal growth factor signaling pathway, Wnt signaling pathway and mammalian target of rapamycin signaling pathway were significantly enriched (P < 0.05). Conclusion hsa-miRNA-451 might be involved in various signaling pathways related to proliferation and development of epithelial cells.

Citation： ChenZhenna, ShaoYan, LiXiaorong. Prediction and bioinformatic analysis of hsa-miRNA-451 target genes. Chinese Journal of Ocular Fundus Diseases, 2015, 31(6): 597-599. doi: 10.3760/cma.j.issn.1005-1015.2015.06.022 Copy

1.	Kato M, Castro NE, Natarajan R. MicroRNAs: potential mediators and biomarkers of diabetic complications[J]. Free Radic Biol Med, 2013, 64:85-94.
2.	Mendell JT, Olson EN. MicroRNAs in stress signaling and human disease[J]. Cell, 2012, 148(6):1172-1187.
3.	康春生, 浦佩玉, 王广秀, 等.靶向表皮生长因子受体的小分子干扰RNA抑制TJ905人脑胶质瘤细胞增殖和侵袭的实验研究[J].中华医学杂志, 2004, 84(18):1503-1508.
4.	Liu X, Zhang X, Xiang J, et al. miR-451: potential role as tumor suppressor of human hepatoma cell growth and invasion[J]. Int J Oncol, 2014, 45(2):739-745.
5.	Kovalchuk O, Filkowski J, Meservy J, et al. Involvement of microRNA-451 in resistance of the MCF-7 breast cancer cells to chemotherapeutic drug doxorubicin[J]. Mol Cancer Ther, 2008, 7(7):2152-2159.
6.	Wang XC, Tian LL, Jiang XY, et al. The expression and function of hsa-miRNA-451 in non-small cell lung cancer[J]. Cancer Lett, 2011, 311(2): 203-209.
7.	Zhang Z, Luo X, Ding S, et al. MicroRNA-451 regulates p38 MAPK signaling by targeting of Ywhaz and suppresses the mesangial hypertrophy in early diabetic nephropathy[J]. FEBS Lett, 2012, 586(1):20-26.
8.	Mastropasqua R, Toto L, Cipollone E, et al. Role of microRNAs in the modulation of diabetic retinopathy[J]. Prog Retin Eye Res, 2014, 43:92-107.
9.	Ashburner M, Ball CA, Blake JA, et al. Gene ontology: tool for the unification of biology.The Gene Ontology Consortium[J].Nat Genet, 2000, 25(1):25-29.
10.	Huang da W, Sherman BT, Lempicki RA. Systematic and integrative analysis of large gene lists using DAVID bioinformatics resources[J]. Nat Protoc, 2009, 4(1): 44-57.
11.	Huang da W, Sherman BT, Lempicki RA.Bioinformatics enrichment tools: paths toward the comprehensive functional analysis of large gene lists[J].Nucleic Acids Res, 2009, 37(1):1-13.
12.	Oberstein SY, Byun J, Herrera D, et al. Cell proliferation in human epiretinal membranes: characterization of cell types and correlation with disease condition and duration[J].Mol Vis, 2011, 17:1794-805.
13.	Reinhart BJ, Slack FJ, Basson M, et al. The 21-nucleotide let-7 RNA regulates developmental timing in Caenorhabditis elegans[J]. Nature, 2000, 403(6772): 901-906.
14.	Griffiths-Jones S, Saini HK, van Dongen S, et al. miRBase: tools for microRNA genomics[J]. Nucleic Acids Res, 2008, 36:154-158.
15.	Kozomara A, Griffiths-Jones S. miRBase: annotating high confidence microRNAs using deep sequencing data[J].Nucleic Acids Res, 2014, 42:68-73.
16.	Enright AJ, John B, Gaul U, et al. MicroRNA targets in Drosophila[J]. Genome Biol, 2003, 5(1):1.
17.	Lewis BP, Shih IH, Jones-Rhoades MW, et al. Prediction of mammalian microRNA targets[J].Cell, 2003, 115(7):787-798.
18.	Lewis BP, Burge CB, Bartel DP. Conserved seed pairing, often flanked by adenosines, indicates that thousands of human genes are microRNA targets[J]. Cell, 2005, 120(1):15-20.
19.	Krek A, Grun D, Poy MN, et al. Combinatorial microRNA target predictions[J]. Nat Genet, 2005, 37(5):495-500.
20.	Wang S, Aurora AB, Johnson BA, et al. The endothelial-specific microRNA miR-126 governs vascular integrity and angiogenesis[J].Dev Cell, 2008, 15(2):261-271.
21.	Kita T, Hata Y, Arita R, et al. Role of TGF-beta in proliferative vitreoretinal diseases and ROCK as a therapeutic target[J]. Proc Natl Acad Sci USA, 2008, 105(45): 17504-17509.
22.	Chan CM, Chang HH, Wang VC, et al. Inhibitory effects of resveratrol on PDGF-BB-induced retinal pigment epithelial cell migration via PDGFRbeta, PI3K/Akt and MAPK pathways[J/OL]. PLoS One, 2013, 8(2):56819[2013-02-14].https://dash.harvard.edu/bitstream/handle/1/10622983/3572951.pdf?sequence=1..

1. Kato M, Castro NE, Natarajan R. MicroRNAs: potential mediators and biomarkers of diabetic complications[J]. Free Radic Biol Med, 2013, 64:85-94.
2. Mendell JT, Olson EN. MicroRNAs in stress signaling and human disease[J]. Cell, 2012, 148(6):1172-1187.
3. 康春生, 浦佩玉, 王广秀, 等.靶向表皮生长因子受体的小分子干扰RNA抑制TJ905人脑胶质瘤细胞增殖和侵袭的实验研究[J].中华医学杂志, 2004, 84(18):1503-1508.
4. Liu X, Zhang X, Xiang J, et al. miR-451: potential role as tumor suppressor of human hepatoma cell growth and invasion[J]. Int J Oncol, 2014, 45(2):739-745.
5. Kovalchuk O, Filkowski J, Meservy J, et al. Involvement of microRNA-451 in resistance of the MCF-7 breast cancer cells to chemotherapeutic drug doxorubicin[J]. Mol Cancer Ther, 2008, 7(7):2152-2159.
6. Wang XC, Tian LL, Jiang XY, et al. The expression and function of hsa-miRNA-451 in non-small cell lung cancer[J]. Cancer Lett, 2011, 311(2): 203-209.
7. Zhang Z, Luo X, Ding S, et al. MicroRNA-451 regulates p38 MAPK signaling by targeting of Ywhaz and suppresses the mesangial hypertrophy in early diabetic nephropathy[J]. FEBS Lett, 2012, 586(1):20-26.
8. Mastropasqua R, Toto L, Cipollone E, et al. Role of microRNAs in the modulation of diabetic retinopathy[J]. Prog Retin Eye Res, 2014, 43:92-107.
9. Ashburner M, Ball CA, Blake JA, et al. Gene ontology: tool for the unification of biology.The Gene Ontology Consortium[J].Nat Genet, 2000, 25(1):25-29.
10. Huang da W, Sherman BT, Lempicki RA. Systematic and integrative analysis of large gene lists using DAVID bioinformatics resources[J]. Nat Protoc, 2009, 4(1): 44-57.
11. Huang da W, Sherman BT, Lempicki RA.Bioinformatics enrichment tools: paths toward the comprehensive functional analysis of large gene lists[J].Nucleic Acids Res, 2009, 37(1):1-13.
12. Oberstein SY, Byun J, Herrera D, et al. Cell proliferation in human epiretinal membranes: characterization of cell types and correlation with disease condition and duration[J].Mol Vis, 2011, 17:1794-805.
13. Reinhart BJ, Slack FJ, Basson M, et al. The 21-nucleotide let-7 RNA regulates developmental timing in Caenorhabditis elegans[J]. Nature, 2000, 403(6772): 901-906.
14. Griffiths-Jones S, Saini HK, van Dongen S, et al. miRBase: tools for microRNA genomics[J]. Nucleic Acids Res, 2008, 36:154-158.
15. Kozomara A, Griffiths-Jones S. miRBase: annotating high confidence microRNAs using deep sequencing data[J].Nucleic Acids Res, 2014, 42:68-73.
16. Enright AJ, John B, Gaul U, et al. MicroRNA targets in Drosophila[J]. Genome Biol, 2003, 5(1):1.
17. Lewis BP, Shih IH, Jones-Rhoades MW, et al. Prediction of mammalian microRNA targets[J].Cell, 2003, 115(7):787-798.
18. Lewis BP, Burge CB, Bartel DP. Conserved seed pairing, often flanked by adenosines, indicates that thousands of human genes are microRNA targets[J]. Cell, 2005, 120(1):15-20.
19. Krek A, Grun D, Poy MN, et al. Combinatorial microRNA target predictions[J]. Nat Genet, 2005, 37(5):495-500.
20. Wang S, Aurora AB, Johnson BA, et al. The endothelial-specific microRNA miR-126 governs vascular integrity and angiogenesis[J].Dev Cell, 2008, 15(2):261-271.
21. Kita T, Hata Y, Arita R, et al. Role of TGF-beta in proliferative vitreoretinal diseases and ROCK as a therapeutic target[J]. Proc Natl Acad Sci USA, 2008, 105(45): 17504-17509.
22. Chan CM, Chang HH, Wang VC, et al. Inhibitory effects of resveratrol on PDGF-BB-induced retinal pigment epithelial cell migration via PDGFRbeta, PI3K/Akt and MAPK pathways[J/OL]. PLoS One, 2013, 8(2):56819[2013-02-14].https://dash.harvard.edu/bitstream/handle/1/10622983/3572951.pdf?sequence=1..

Previous Article
高血压患者眼底血管改变与高血压病情和左心室肥厚的相关性分析
Next Article
疟原虫感染后的双眼视神经炎一例

Chinese Journal of Ocular Fundus Diseases

Prediction and bioinformatic analysis of hsa-miRNA-451 target genes

Abstract Full text Figures/Tables Video References Cited by

Previous Article

Next Article

Format

Content