Effect of panretinal photocoagulation on the expression of cyclooxygenase-2, vascular endothelial cell growth factor in epiretinal membrane of proliferative diabetic retinopathy_Chinese Journal of Ocular Fundus Diseases

Authors：

HanLinfeng ,  KeGenjie , WangLin , GuYonghao , GuQihong , DongKai , LiuJiajia

Department of Ophthalmology, Anhui Provincial Hospital, Hefei 230001, China;

Corresponding author：

KeGenjie, Email: kegenjie@163.com

Keywords：

Diabetic retinopathy/therapy; Laser coagulation/adverse effects; Cyclooxygenase 2; Vascular endothelial growth factors

DOI：

10.3760/cma.j.issn.1005-1015.2016.02.007

Video：

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Objective To observe the effect of panretinal photocoagulation (PRP) on the expression of cyclooxygenase-2 (COX-2), vascular endothelial cell growth factor (VEGF) in epiretinal membrane of proliferative diabetic retinopathy (PDR). Methods Atotal of 35 patients (35 eyes) with PDR and underwent plana vitrectomy were enrolled in this study. The patients were divided into non-PRP group (19 patients, 19 eyes) and PRP group (16 patients, 16 eyes) depends on if they had received PRP before surgery. The epiretinal membranes stripped during operation were collected for pathological examination. The histopathological features was observed by haematoxylin and eosin stain. The expression of CD34, COX-2 and VEGF, and microvessel density (MVD) were measured by immunohistochemistry method. Results Many new dispersed capillary blood vessels were found in the thick epiretinal membranes of non-PRP group, while scattered small blood vessels were found in the relatively thin epiretinal membranes of PRP group. MVD value was (7.42±1.39) in the non-PRP group and (4.56±1.22) in the PRP group, which was lower than the non-PRP group (t=6.41, P<0.01). The expression of CD34, COX-2 and VEGF in the tissues of epiretinal membrane in PRP group were obviously lower than the non-PRP group (t=6.147, 5.944, 7.445; P<0.01). Conclusion PRP can effectively inhibit the expression of COX-2 and VEGF in epiretinal membrane of PRP patients.

Citation： HanLinfeng, KeGenjie, WangLin, GuYonghao, GuQihong, DongKai, LiuJiajia. Effect of panretinal photocoagulation on the expression of cyclooxygenase-2, vascular endothelial cell growth factor in epiretinal membrane of proliferative diabetic retinopathy. Chinese Journal of Ocular Fundus Diseases, 2016, 32(2): 140-143. doi: 10.3760/cma.j.issn.1005-1015.2016.02.007 Copy

1.	Chong V. Biological, preclinical and clinical characteristics of inhibitors of vascular endothelial growth factors[J]. Ophthalmologica, 2012,227 Suppl 1:S2-10. DOI: 10.1159/000337152.
2.	Lee SH, Lee YS, Hong YG, et al. Significance of COX-2 and VEGF expression in histopathologic grading and invasiveness of meningiomas[J]. APMIS, 2014,122(1):16-24. DOI:10.1111/apm.12079.
3.	Maia OJ, Takahashi BS, Costa RA, et al. Combined laser and intravitreal triamcinolone for proliferative diabetic retinopathy and macular edema: one-year results of a randomized clinical trial[J]. Am J Ophthalmol, 2009,147(2):291-297. DOI: 10.1016/j.ajo.2008.08.024.
4.	付浴东, 王萍, 江莉, 等. 玻璃体腔注射雷珠单抗、全视网膜激光光凝对增生型糖尿病视网膜病变患眼玻璃体切割手术及治疗效果的影响[J]. 中华眼底病杂志, 2015,31(2):143-146. DOI:10.3760/cma.j.issn.1005-1015.2015.02.008.Fu YD,Wang P,Jiang L,et al.The effect of intravitreal injection of ranibizumab and pan-retina photocoagulation in the treatment of severe proliferative diabetic retinopathy wih 23G vitreoretinal surgery[J].Chin J Ocul Fundus Dis,2015,31(2): 143-146. DOI:10.3760/ cma.j.issn. 1005-1015.2015.02.008.
5.	Wilkinson CP, Ferris FR, Klein RE, et al. Proposed international clinical diabetic retinopathy and diabetic macular edema disease severity scales[J]. Ophthalmology, 2003,110(9):1677-1682.
6.	林冰,刘晓玲.眼底病激光光凝治疗操作技术要点[J]. 中华眼底病杂志, 2013,29(5): 526-528. DOI:10.3760/cma.j.issn.1005-1015.2013.05.021.Lin B,Liu XL. The main technical points of ophthalmology laser photocoagulation treatment operation[J].Chin J Ocul Fundus Dis,2013,29(5): 526-528. DOI: 10.3760/cma.j.issn.1005-1015.2013.05.021.
7.	Weidner N. Intratumor microvessel density as a prognostic factor in cancer[J]. Am J Pathol, 1995,147(1):9-19.
8.	Kraan MC, Smith MD, Weedon H, et al. Measurement of cytokine and adhesion molecule expression in synovial tissue by digital image analysis[J]. Ann Rheum Dis, 2001,60(3):296-298.
9.	Goh PP, Omar MA, Yusoff AF. Diabetic eye screening in Malaysia: findings from the National Health and Morbidity Survey 2006[J]. Singapore Med J, 2010,51 (8): 631-634.
10.	李慧玲, 张文莲, 赵英芳. 病理诊断中免疫组织化学技术常见问题及改进[J]. 包头医学院学报, 2012,28(1):124-125. DOI：10.3969/j.issn.1006-740X.2012.01.071.Li HL,Zhang WL,Zhao YF. The pathological diagnosis of immunohistochemical technology common problems and improvement [J]. Journal of Baotou Medical College, 2012,28(1):124-125. DOI：10.3969/j.issn.1006-740X.2012.01.071.
11.	Moon HS, Park WI, Sung SH, et al. Immunohistochemical and quantitative competitive PCR analyses of midkine and pleiotrophin expression in cervical cancer[J]. Gynecol Oncol, 2003,88(3):289-297.
12.	Muqit MM, Sanghvi C, McLauchlan R, et al. Study of clinical applications and safety for Pascal(R) laser photocoagulation in retinal vascular disorders[J]. Acta Ophthalmol, 2012,90(2):155-161. DOI: 10.1111/j.1755-3768.2009.01854.x.
13.	Chen CH, Chen YH, Wu PC, et al. Treatment of branch retinal vein occlusion induced macular edema in treatment-naive cases with a single intravitreal triamcinolone or bevacizumab injection[J]. Chang Gung Med J, 2010,33(4):424-435.
14.	Feng J, Zhao T, Zhang Y, et al. Differences in aqueous concentrations of cytokines in macular edema secondary to branch and central retinal vein occlusion[J/OL]. PLoS One, 2013,8(7):68149.[2013-07-05]. http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3702559/. DOI:10.1371/journal.pone.0068149.
15.	贺涛, 刑怡桥, 艾明, 等. 诱生型一氧化氮合酶及环氧化酶一2对氧诱导视网膜病变小鼠模型新生血管形成的影响[J]. 中华眼底病杂志, 2008,24(1):49-52.He T,Xing YQ,Ai M,et al. Effect of inducible nitric oxide synthase or cyclooxygenase-2 on retinal neovascularization in oxygen-induced retinopathy mouse model[J].Chin J Ocul Fundus Dis,2008,24(1):49-52.
16.	王友, 邓铂林, 黄健, 等. 玻璃体腔注射雷珠单抗治疗严重增生型糖尿病视网膜病变后玻璃体细胞因子的变化[J]. 中华眼底病杂志, 2014,30(5):484-487. DOI: 10.3760/ cma. j.issn.1005-1015.2014.05.014.Wang Y, Deng BL,Huang J,et al. Concentration of the inflammatory cytokines in vitreous of severe proliferative diabetic retinopathy after intravitreal ranibizumab injection[J].Chin J Ocul Fundus Dis,2014,30(5):484-487.DOI: 10.3760/cma.j.issn. 1005-1015.2014.05.014.
17.	Pece A, Isola V, Piermarocchi S, et al. Efficacy and safety of anti-vascular endothelial growth factor (VEGF) therapy with intravitreal ranibizumab (Lucentis) for naive retinal vein occlusion: 1-year follow-up[J]. Br J Ophthalmol, 2011,95(1):56-68. DOI:10.1136/bjo.2009.174060.
18.	Rouvas A, Petrou P, Ntouraki A, et al. Intravitreal ranibizumab (Lucentis) for branch retinal vein occlusion-induced macular edema: nine-month results of a prospective study[J]. Retina, 2010,30(6):893-902. DOI:10.1097/IAE.0b013e3181c d4894.

1. Chong V. Biological, preclinical and clinical characteristics of inhibitors of vascular endothelial growth factors[J]. Ophthalmologica, 2012,227 Suppl 1:S2-10. DOI: 10.1159/000337152.
2. Lee SH, Lee YS, Hong YG, et al. Significance of COX-2 and VEGF expression in histopathologic grading and invasiveness of meningiomas[J]. APMIS, 2014,122(1):16-24. DOI:10.1111/apm.12079.
3. Maia OJ, Takahashi BS, Costa RA, et al. Combined laser and intravitreal triamcinolone for proliferative diabetic retinopathy and macular edema: one-year results of a randomized clinical trial[J]. Am J Ophthalmol, 2009,147(2):291-297. DOI: 10.1016/j.ajo.2008.08.024.
4. 付浴东, 王萍, 江莉, 等. 玻璃体腔注射雷珠单抗、全视网膜激光光凝对增生型糖尿病视网膜病变患眼玻璃体切割手术及治疗效果的影响[J]. 中华眼底病杂志, 2015,31(2):143-146. DOI:10.3760/cma.j.issn.1005-1015.2015.02.008.Fu YD,Wang P,Jiang L,et al.The effect of intravitreal injection of ranibizumab and pan-retina photocoagulation in the treatment of severe proliferative diabetic retinopathy wih 23G vitreoretinal surgery[J].Chin J Ocul Fundus Dis,2015,31(2): 143-146. DOI:10.3760/ cma.j.issn. 1005-1015.2015.02.008.
5. Wilkinson CP, Ferris FR, Klein RE, et al. Proposed international clinical diabetic retinopathy and diabetic macular edema disease severity scales[J]. Ophthalmology, 2003,110(9):1677-1682.
6. 林冰,刘晓玲.眼底病激光光凝治疗操作技术要点[J]. 中华眼底病杂志, 2013,29(5): 526-528. DOI:10.3760/cma.j.issn.1005-1015.2013.05.021.Lin B,Liu XL. The main technical points of ophthalmology laser photocoagulation treatment operation[J].Chin J Ocul Fundus Dis,2013,29(5): 526-528. DOI: 10.3760/cma.j.issn.1005-1015.2013.05.021.
7. Weidner N. Intratumor microvessel density as a prognostic factor in cancer[J]. Am J Pathol, 1995,147(1):9-19.
8. Kraan MC, Smith MD, Weedon H, et al. Measurement of cytokine and adhesion molecule expression in synovial tissue by digital image analysis[J]. Ann Rheum Dis, 2001,60(3):296-298.
9. Goh PP, Omar MA, Yusoff AF. Diabetic eye screening in Malaysia: findings from the National Health and Morbidity Survey 2006[J]. Singapore Med J, 2010,51 (8): 631-634.
10. 李慧玲, 张文莲, 赵英芳. 病理诊断中免疫组织化学技术常见问题及改进[J]. 包头医学院学报, 2012,28(1):124-125. DOI：10.3969/j.issn.1006-740X.2012.01.071.Li HL,Zhang WL,Zhao YF. The pathological diagnosis of immunohistochemical technology common problems and improvement [J]. Journal of Baotou Medical College, 2012,28(1):124-125. DOI：10.3969/j.issn.1006-740X.2012.01.071.
11. Moon HS, Park WI, Sung SH, et al. Immunohistochemical and quantitative competitive PCR analyses of midkine and pleiotrophin expression in cervical cancer[J]. Gynecol Oncol, 2003,88(3):289-297.
12. Muqit MM, Sanghvi C, McLauchlan R, et al. Study of clinical applications and safety for Pascal(R) laser photocoagulation in retinal vascular disorders[J]. Acta Ophthalmol, 2012,90(2):155-161. DOI: 10.1111/j.1755-3768.2009.01854.x.
13. Chen CH, Chen YH, Wu PC, et al. Treatment of branch retinal vein occlusion induced macular edema in treatment-naive cases with a single intravitreal triamcinolone or bevacizumab injection[J]. Chang Gung Med J, 2010,33(4):424-435.
14. Feng J, Zhao T, Zhang Y, et al. Differences in aqueous concentrations of cytokines in macular edema secondary to branch and central retinal vein occlusion[J/OL]. PLoS One, 2013,8(7):68149.[2013-07-05]. http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3702559/. DOI:10.1371/journal.pone.0068149.
15. 贺涛, 刑怡桥, 艾明, 等. 诱生型一氧化氮合酶及环氧化酶一2对氧诱导视网膜病变小鼠模型新生血管形成的影响[J]. 中华眼底病杂志, 2008,24(1):49-52.He T,Xing YQ,Ai M,et al. Effect of inducible nitric oxide synthase or cyclooxygenase-2 on retinal neovascularization in oxygen-induced retinopathy mouse model[J].Chin J Ocul Fundus Dis,2008,24(1):49-52.
16. 王友, 邓铂林, 黄健, 等. 玻璃体腔注射雷珠单抗治疗严重增生型糖尿病视网膜病变后玻璃体细胞因子的变化[J]. 中华眼底病杂志, 2014,30(5):484-487. DOI: 10.3760/ cma. j.issn.1005-1015.2014.05.014.Wang Y, Deng BL,Huang J,et al. Concentration of the inflammatory cytokines in vitreous of severe proliferative diabetic retinopathy after intravitreal ranibizumab injection[J].Chin J Ocul Fundus Dis,2014,30(5):484-487.DOI: 10.3760/cma.j.issn. 1005-1015.2014.05.014.
17. Pece A, Isola V, Piermarocchi S, et al. Efficacy and safety of anti-vascular endothelial growth factor (VEGF) therapy with intravitreal ranibizumab (Lucentis) for naive retinal vein occlusion: 1-year follow-up[J]. Br J Ophthalmol, 2011,95(1):56-68. DOI:10.1136/bjo.2009.174060.
18. Rouvas A, Petrou P, Ntouraki A, et al. Intravitreal ranibizumab (Lucentis) for branch retinal vein occlusion-induced macular edema: nine-month results of a prospective study[J]. Retina, 2010,30(6):893-902. DOI:10.1097/IAE.0b013e3181c d4894.

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Effect of panretinal photocoagulation on the expression of cyclooxygenase-2, vascular endothelial cell growth factor in epiretinal membrane of proliferative diabetic retinopathy

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