Enhancing the research on three-dimensional culture of embryonic stem cells to optimize the strategy to make the seed cells_Chinese Journal of Ocular Fundus Diseases

Authors：

WuWei ,  YinZhengqin

Eye Branch of Southwest Hospital of the Third Military Medical University, Chongqing 400038, China;

Corresponding author：

YinZhengqin, Email: yzhengqin@163.com

Keywords：

Embryonic stem cells; Adult stem cells; Cell culture techniques; Editorial

DOI：

10.3760/cma.j.issn.1005-1015.2016.06.003

Video：

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There are over 8 million blind patients in China, 1/3 of them are suffered from retinal degeneration diseases. Stem cells transplantation can delay the photoreceptor cell degeneration or replace the dead photoreceptor cells, provides hopes for these patients. How to make enough seed cells is the major barrier for cell therapy. Good seed cells should be safe and with great pluripotency, and can be made from a wide range of sources, easy to be standardized and industrialized. Seed cells made from three-dimensional embryonic stem cells cultures can reach the above criteria, thus three-dimensional embryonic stem cell culture is a new strategy for making seed cells for cell treatment of blind diseases.

Citation： WuWei, YinZhengqin. Enhancing the research on three-dimensional culture of embryonic stem cells to optimize the strategy to make the seed cells. Chinese Journal of Ocular Fundus Diseases, 2016, 32(6): 573-575. doi: 10.3760/cma.j.issn.1005-1015.2016.06.003 Copy

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6.	Shih CC, Forman SJ, Chu P, et al. Human embryonic stem cells are prone to generate primitive, undifferentiated tumors in engrafted human fetal tissues in severe combined immunodeficient mice[J]. Stem Cells Dev, 2007, 16(6):893-902. DOI:10.1089/scd.2007.0070(2007).
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10.	Zhou PY, Peng GH, Xu H, et al. c-Kit⁺ cells isolated from human fetal retinas represent a new population of retinal progenitor cells[J]. J Cell Sci, 2015, 128(11):2169-2178. DOI:10.1242/jcs.169086.
11.	阴正勤, 李世迎, 蹇骞.眼科干细胞研究的现状及进一步研究的问题[J].中华实验眼科杂志, 2015, 33(9):769-773. DOI:10.3760/cma.j.issn.2095-0160.2015.09.001.Yin ZQ, Li SY, Jian Q. The current study, challenges and application of stem cell transplantation in ophthalmology[J].Chin J Exp Ophthalmol, 2015, 33(9):769-773. DOI:10.3760/cma.j.issn.2095-0160.2015.09.001.
12.	Liu DN, Yin ZQ, Wu N, et al. Rat bone marrow stromal cells express retinal phenotypic markers following different induction protocols[J]. Ophthalmic Res, 2009, 41(4):186-193. DOI:10.1159/000217722.
13.	Duan P, Xu H, Zeng Y, et al. Human bone marrow stromal cells can differentiate to a retinal pigment epithelial phenotype when co-cultured with pig retinal pigment epithelium using a transwell system[J]. Cell Physiol Biochem, 2013, 31(4):601-613. DOI:10.1159/000350080.
14.	Eiraku M, Takata N, Ishibashi H, et al. Self-organizing optic-cup morphogenesis in three-dimensional culture[J]. Nature, 2011, 472(7341):51-56. DOI:10.1038/nature09941.
15.	Nakano T, Ando S, Takata N, et al. Self-formation of optic cups and storable stratified neural retina from human ESCs[J]. Cell Stem Cell, 2012, 10(6):771-785. DOI:10.1016/j.stem.2012.05.009.
16.	Wu W, Zeng Y, Li Z, et al. Features specific to retinal pigment epithelium cells derived from three-dimensional human embryonic stem cell cultures-a new donor for cell therapy[J]. Oncotarget, 2016, 7(16):22819-22833. DOI:10.18632/oncotarget. 8185.
17.	Gao L, Chen X, Zeng Y, et al. Intermittent high oxygen influences the formation of neural retinal tissue from human embryonic stem cells[J/OL]. Sci Rep, 2016, 6, 29944[2016-07-20].http://www.nature.com/articles/srep29944. DOI:10.1038/srep29944.

1. Thomson JA, Itskovitz-Eldor J, Shapiro SS, et al. Embryonic stem cell lines derived from human blastocysts[J]. Science, 1998, 282(5391):1145-1147.
2. Idelson M, Alper R, Obolensky A, et al. Directed differentiation of human embryonic stem cells into functional retinal pigment epithelium cells[J]. Cell Stem Cell, 2009, 5(4):396-408. DOI:10.1016/j.stem.2009.07.002.
3. Osakada F, Ikeda H, Mandai M, et al. Toward the generation of rod and cone photoreceptors from mouse, monkey and human embryonic stem cells[J].Nat Biotechnol, 2008, 26(2):215-224. DOI:10.1038/nbt1384.
4. Schwartz SD, Regillo CD, Lam BL, et al. Human embryonic stem cell-derived retinal pigment epithelium in patients with age-related macular degeneration and Stargardt's macular dystrophy:follow-up of two open-label phase 1/2 studies[J]. Lancet, 2015, 385(9967):509-516. DOI:10.1016/S0140-6736(14)61376-3.
5. Yin ZQ, Liu Y, Li S, et al. Clincal trial:subretinal transplantation of CTS hESC derived RPE in the treatment of wet age-related macular degeneration (wAMD)[J]. Invest Ophthalmol Vis Sci, 2016, 57(12):3742.
6. Shih CC, Forman SJ, Chu P, et al. Human embryonic stem cells are prone to generate primitive, undifferentiated tumors in engrafted human fetal tissues in severe combined immunodeficient mice[J]. Stem Cells Dev, 2007, 16(6):893-902. DOI:10.1089/scd.2007.0070(2007).
7. Li Z, Zeng Y, Chen X, et al. Neural stem cells transplanted to the subretinal space of rd1 mice delay retinal degeneration by suppressing microglia activation[J]. Cytotherapy, 2016, 18(6):771-784. DOI:10.1016/j.jcyt.2016.03.001.
8. Tian C, Weng CC, Yin ZQ. BDNF improves the efficacy ERG amplitude maintenance by transplantation of retinal stem cells in RCS rats[J]. Adv Exp Med Biol, 2010, 664:375-384. DOI:10.1007/978-1-4419-1399-9_43.
9. Nazari H, Zhang L, Zhu D, et al. Stem cell based therapies for age-related macular degeneration:the promises and the challenges[J]. Prog Retin Eye Res, 2015, 48:1-39. DOI:10.1016/j.preteyeres.2015.06.004.
10. Zhou PY, Peng GH, Xu H, et al. c-Kit⁺ cells isolated from human fetal retinas represent a new population of retinal progenitor cells[J]. J Cell Sci, 2015, 128(11):2169-2178. DOI:10.1242/jcs.169086.
11. 阴正勤, 李世迎, 蹇骞.眼科干细胞研究的现状及进一步研究的问题[J].中华实验眼科杂志, 2015, 33(9):769-773. DOI:10.3760/cma.j.issn.2095-0160.2015.09.001.Yin ZQ, Li SY, Jian Q. The current study, challenges and application of stem cell transplantation in ophthalmology[J].Chin J Exp Ophthalmol, 2015, 33(9):769-773. DOI:10.3760/cma.j.issn.2095-0160.2015.09.001.
12. Liu DN, Yin ZQ, Wu N, et al. Rat bone marrow stromal cells express retinal phenotypic markers following different induction protocols[J]. Ophthalmic Res, 2009, 41(4):186-193. DOI:10.1159/000217722.
13. Duan P, Xu H, Zeng Y, et al. Human bone marrow stromal cells can differentiate to a retinal pigment epithelial phenotype when co-cultured with pig retinal pigment epithelium using a transwell system[J]. Cell Physiol Biochem, 2013, 31(4):601-613. DOI:10.1159/000350080.
14. Eiraku M, Takata N, Ishibashi H, et al. Self-organizing optic-cup morphogenesis in three-dimensional culture[J]. Nature, 2011, 472(7341):51-56. DOI:10.1038/nature09941.
15. Nakano T, Ando S, Takata N, et al. Self-formation of optic cups and storable stratified neural retina from human ESCs[J]. Cell Stem Cell, 2012, 10(6):771-785. DOI:10.1016/j.stem.2012.05.009.
16. Wu W, Zeng Y, Li Z, et al. Features specific to retinal pigment epithelium cells derived from three-dimensional human embryonic stem cell cultures-a new donor for cell therapy[J]. Oncotarget, 2016, 7(16):22819-22833. DOI:10.18632/oncotarget. 8185.
17. Gao L, Chen X, Zeng Y, et al. Intermittent high oxygen influences the formation of neural retinal tissue from human embryonic stem cells[J/OL]. Sci Rep, 2016, 6, 29944[2016-07-20].http://www.nature.com/articles/srep29944. DOI:10.1038/srep29944.

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