Expression of triggering receptor expressed on myeloid cells in ischemic optic neuropathy_Chinese Journal of Ocular Fundus Diseases

Authors：

 Meng Fanchao , Sun Shilong , Su Jian , Shi Peiyu , Zhou Weimin , Wang Shuxiang , Chu Jinting

Department of Neurology, Zhengzhou Second Hospital, Zhengzhou 450006, China;

Corresponding author：

Meng Fanchao, Email: mfcyx@126.com

Keywords：

Optic neuropathy, ischemic; Triggering receptors expressed by myeloid cells; Caspase-3; IL-6; Animal experimentation

DOI：

10.3760/cma.j.issn.1005-1015.2018.05.012

Video：

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Abstract Full text Figures/Tables Video References Cited by

Objective To observe the expression of triggering receptor expressed on myeloid cells (TREM), Caspase-3 and interleukin (IL)-6 in optic nerve tissue of ischemic optic neuropathy (ION). Methods Twenty Sprague-Dawley rats were randomly divided into control group and model group, 10 rats in each group. The permanent ligation of bilateral internal carotid arteries (BICA) was performed for 14 days to establish sub-acute ION model as model group. The control group were separated BICA without ligation. The expressions of TREM-1, TREM-2, Caspase-3 and IL-6 in rat retina were detected by reverse transcription PCR and Western blot, respectively. ResultsCompared with the control group, the expressions of TREM-1, Caspase-3, IL-6 mRNA (t=6.058, 7.86, 6.055) and protein (t=9.671, 9.524, 14.501) in the optic nerve tissue of the model group were increased, while the expression of TREM-2 mRNA and protein (t=9.283) was decreased, and the difference was statistically significant (P＜0.05). Conclusion In ischemic optic nerve tissue, TREM-1 mRNA and protein were significantly expressed, the expressions of TREM-2 mRNA and protein decreased significantly.

Citation： Meng Fanchao, Sun Shilong, Su Jian, Shi Peiyu, Zhou Weimin, Wang Shuxiang, Chu Jinting. Expression of triggering receptor expressed on myeloid cells in ischemic optic neuropathy. Chinese Journal of Ocular Fundus Diseases, 2018, 34(5): 471-474. doi: 10.3760/cma.j.issn.1005-1015.2018.05.012 Copy

1.	Ford JW, McVicar DW. TREM and TREM-like receptors in inflammation and disease[J].Curr Opin Immunol, 2009, 21(1): 38-46. DOI:10.1016/j.coi.2009.01.009.
2.	Bouchon A, Dietrich J, Colonna M. Cutting edge: inflammatory responses can be triggered by TREM-1, a novel receptor expressed on neutrophils and monocytes[J]. J Immunol, 2000, 164: 4991-4995.
3.	Sieber MW, Jaenisch N, Brehm M, et al. Attenuated inflammatory response in triggering receptor expressed on myeloid cells 2(TREM2) knock-out mice following stroke[J/OL]. PLoS One, 2013, 8(1): 52982[2013-01-03] . https://doi.org/10.1371/journal.pone.0052982. DOI:10.1371/journal.pone.0052982.
4.	Takahashi K, Rochford CDP, Neumann H. Clearance of apoptotic neurons without inflammation by microglial triggering receptor expressed on myeloid cells-2[J]. Exp Med, 2005, 201(4): 647-657. DOI:10.1084/jem.20041611.
5.	Kawabori M, Kacimi R, Kauppinen T, et al. Triggering receptor expressed on myeloid cells 2(TREM2) deficiency attenuates phagocytic activities of microglia and exacerbates ischemic damage in experimental stroke[J]. J Neurosci, 2015, 35(8): 3384-3396. DOI:10.1523/JNEUROSCI. 2620-14.2015.
6.	Peng Q, Malhotra S, Torchia JA, et al. TREM2-and DAP12-dependent activation of PI3K requires DAP10 and is inhibited by SHIP1[J]. Sci Signal, 2010, 3(122): 38.DOI: 10.1126/scisignal.2000500.
7.	Colonna M. TREMs in the immune system and beyond[J]. Nat Rev Immunol,2003, 3(6): 445- 453. DOI: 10.1038/nri1106.
8.	Colonna M, Facchetti F. TREM-1(triggering receptor expressed on myeloid cells): a new player in acute inflammatory responses[J].J Infect Dis, 2003, 187 Suppl 2:S397-401. DOI:10.1086/374754.
9.	Sharif O, Knapp S. From expression to signaling: roles of TREM-1 and TREM-2 in innate immunity and bacterial infection[J]. Immunobiology, 2008, 213(9-10): 701-713. DOI:10.1111/jre.12417.
10.	Fahrenhold M, Rakic S, Classey J,et al.TREM2 expression in the human brain: a marker of monocyte recruitment?[J/OL].Brain Pathol,2017,2017:E1[2017-10-07] .https://doi.org/10.1111/bpa.12564. DOI: 10.1111/bpa.12564.[published online ahead of print].
11.	.Bleharski JR, Kiessler V, Buonsanti C, et al. A role for triggering receptor expressed on myeloid cells-1 in host defense during the early-induced and adaptive phases of the immune response[J]. J Immunol, 2003,170(7): 3812-3818.
12.	Knapp S, Gibot S, de Vos A, et al. Cutting edge: Expression patterns of surface and soluble triggering receptor expressed on myeloid cells-1 in human endotoxemia[J].J Immunol,2004,173(12): 7131-7134.
13.	Hiroyuki K, Hiroshi K. Microglial TREM2/DAP12 signaling: a double-edged sword in neural diseases[J]. Front Cell Neurosci, 2018, 12:206. DOI:org/10.3389/fncel.2018.00206.
14.	Jeremie J, Stephane P, Lynda Z, et al. Genetic and pharmacological inhibition of TREM-1 limits the development of experimental atherosclerosis[J]. J Am Coll Cardiol, 2016,68(25): 2776-2793. DOI:10.1016/j.jacc.2016.10.015.
15.	Ma Y,Wang J,Wang Y, et al. The biphasic function of microglia in ischemic stroke[J]. Prog Neurobiol, 2017, 157: 247-272. DOI:10.1016/j.pneurobio.2016.01.005.

1. Ford JW, McVicar DW. TREM and TREM-like receptors in inflammation and disease[J].Curr Opin Immunol, 2009, 21(1): 38-46. DOI:10.1016/j.coi.2009.01.009.
2. Bouchon A, Dietrich J, Colonna M. Cutting edge: inflammatory responses can be triggered by TREM-1, a novel receptor expressed on neutrophils and monocytes[J]. J Immunol, 2000, 164: 4991-4995.
3. Sieber MW, Jaenisch N, Brehm M, et al. Attenuated inflammatory response in triggering receptor expressed on myeloid cells 2(TREM2) knock-out mice following stroke[J/OL]. PLoS One, 2013, 8(1): 52982[2013-01-03] . https://doi.org/10.1371/journal.pone.0052982. DOI:10.1371/journal.pone.0052982.
4. Takahashi K, Rochford CDP, Neumann H. Clearance of apoptotic neurons without inflammation by microglial triggering receptor expressed on myeloid cells-2[J]. Exp Med, 2005, 201(4): 647-657. DOI:10.1084/jem.20041611.
5. Kawabori M, Kacimi R, Kauppinen T, et al. Triggering receptor expressed on myeloid cells 2(TREM2) deficiency attenuates phagocytic activities of microglia and exacerbates ischemic damage in experimental stroke[J]. J Neurosci, 2015, 35(8): 3384-3396. DOI:10.1523/JNEUROSCI. 2620-14.2015.
6. Peng Q, Malhotra S, Torchia JA, et al. TREM2-and DAP12-dependent activation of PI3K requires DAP10 and is inhibited by SHIP1[J]. Sci Signal, 2010, 3(122): 38.DOI: 10.1126/scisignal.2000500.
7. Colonna M. TREMs in the immune system and beyond[J]. Nat Rev Immunol,2003, 3(6): 445- 453. DOI: 10.1038/nri1106.
8. Colonna M, Facchetti F. TREM-1(triggering receptor expressed on myeloid cells): a new player in acute inflammatory responses[J].J Infect Dis, 2003, 187 Suppl 2:S397-401. DOI:10.1086/374754.
9. Sharif O, Knapp S. From expression to signaling: roles of TREM-1 and TREM-2 in innate immunity and bacterial infection[J]. Immunobiology, 2008, 213(9-10): 701-713. DOI:10.1111/jre.12417.
10. Fahrenhold M, Rakic S, Classey J,et al.TREM2 expression in the human brain: a marker of monocyte recruitment?[J/OL].Brain Pathol,2017,2017:E1[2017-10-07] .https://doi.org/10.1111/bpa.12564. DOI: 10.1111/bpa.12564.[published online ahead of print].
11. .Bleharski JR, Kiessler V, Buonsanti C, et al. A role for triggering receptor expressed on myeloid cells-1 in host defense during the early-induced and adaptive phases of the immune response[J]. J Immunol, 2003,170(7): 3812-3818.
12. Knapp S, Gibot S, de Vos A, et al. Cutting edge: Expression patterns of surface and soluble triggering receptor expressed on myeloid cells-1 in human endotoxemia[J].J Immunol,2004,173(12): 7131-7134.
13. Hiroyuki K, Hiroshi K. Microglial TREM2/DAP12 signaling: a double-edged sword in neural diseases[J]. Front Cell Neurosci, 2018, 12:206. DOI:org/10.3389/fncel.2018.00206.
14. Jeremie J, Stephane P, Lynda Z, et al. Genetic and pharmacological inhibition of TREM-1 limits the development of experimental atherosclerosis[J]. J Am Coll Cardiol, 2016,68(25): 2776-2793. DOI:10.1016/j.jacc.2016.10.015.
15. Ma Y,Wang J,Wang Y, et al. The biphasic function of microglia in ischemic stroke[J]. Prog Neurobiol, 2017, 157: 247-272. DOI:10.1016/j.pneurobio.2016.01.005.

Chinese Journal of Ocular Fundus Diseases

Expression of triggering receptor expressed on myeloid cells in ischemic optic neuropathy

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