Effect of Tetramethylpyrazine and Rat CTGF miRNA Plasmids on Connective Tissue Growth Factor, Transforming Growth Factor-beta in High Glucose Stimulated Hepatic Stellate Cells_Journal of Biomedical Engineering

Authors：

YANGHong ¹ , LIJun ² , XINGNini ¹ , XIANGYing ¹ , SHENYan ¹ ,  LIXiaosheng ¹

1. Department of Gastroenterology, the Second Affiliated Hospital, Chongqing Medical University, Chongqing 400010, China;
2. Department of Stomatology, the Affiliated Hospital of the Stomatology, Chongqing Medical University, Chongqing 401147, China;

Corresponding author：

LIXiaosheng, Email: LXS6896@126.com

Keywords：

tetramethylpyrazine; hepatic stellate cell; connective tissue growth factor; transforming growth factor-beta

DOI：

10.7507/1001-5515.20140074

Video：

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The aim of this research is to evaluate the effect of tetramethylpyrazine (TMP) and connective tissue growth factor (CTGF) miRNA plasmids on the expressive levels of CTGF, transforming growth factor-beta (TGF-beta) and type Ⅰ collagen of rat hepatic stellate cells (HSC) which are stimulated by high glucose. The rat HSCs which were successfully transfected rat CTGF miRNA plasmids and the rat HSCs which were successfully transfected negative plasmids were cultured in vitro. After stimulus of the TMP and the high glucose, the protein levels and gene expressive levels of CTGF, TGF-beta and type Ⅰ collagen were tested. The results indicated that high glucose increased the expression of CTGF mRNA, CTGF protein, TGF-beta mRNA,TGF-beta protein and type Ⅰ collagen (P<0.05). The expressive levels of CTGF mRNA, CTGF protein, TGF-beta mRNA, TGF-beta and type Ⅰ collagen in TMP group were lower than those in high glucose group and showed statistically significant differences (P0.05). Compared with high glucose group, the expressive levels of CTGF mRNA, CTGF protein, TGF-beta mRNA, TGF-beta and type Ⅰ collagen in rat CTGF miRNA plasmid interference group were significantly lower (P<0.05). However, no statistically significant difference was found in CTGF mRNA and CTGF protein levels between TMP group and CTGF miRNA group (P>0.05), while type Ⅰ collagen levels showed statistically significant differences (P<0.05). It is concluded that high glucose could promote the expressions of CTGF, TGF-beta and type Ⅰ collagen, and TMP and rat CTGF miRNA plasmids could reduce the expressions of CTGF, TGF-beta, type Ⅰ collagen.

Citation： YANGHong, LIJun, XINGNini, XIANGYing, SHENYan, LIXiaosheng. Effect of Tetramethylpyrazine and Rat CTGF miRNA Plasmids on Connective Tissue Growth Factor, Transforming Growth Factor-beta in High Glucose Stimulated Hepatic Stellate Cells. Journal of Biomedical Engineering, 2014, 31(2): 394-399. doi: 10.7507/1001-5515.20140074 Copy

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1. FRIEDMAN S L,BANSAL M B.Reversal of hepatic fibrosis——fact or fantasy?[J].Hepatology,2006,43(2 Suppl 1):S82-S88.
2. LEWINDON P J,PEREIRA T N,HOSKINS A C,et al.The role of hepatic stellate cells and transforming growth factor-β1 in cystic fibrosis liver disease[J].Am J Pathol,2002,160(5):1705-1715.
3. 李芹,邓存良,陈庄,等.高糖、胰岛素对大鼠肝星状细胞增殖影响[J].中国现代医学杂志,2009,19(11):1669-1672,1679.
4. PARADIS V,PERLEMUTER G,BONVOUST F,et al.High glucose and hyperinsulinemia stimulate connective tissue growth factor expression:a potential mechanism involved in progression to fibrosis in nonalcoholic steatohepatitis[J].Hepatology,2001,34(4 Pt 1):738-744.
5. 王文丽,李孝生,陈爽.川芎嗪对肝星状细胞增殖及结缔组织生长因子表达的影响[J].中国生物制品学杂志,2009,22(1):65-68,77.
6. 陈爽,李孝生,王文丽.川芎嗪对转化因子β1刺激肝星状细胞CTGF及Ⅰ型胶原表达的影响[J].生命科学研究,2009,13(2):146-151.
7. KUIPER E J,WITMER A N,KLAASSEN I,et al.Differential expression of connective tissue growth factor in microglia and pericytes in the human diabetic retina[J].Br J Ophthalmol,2004,88(8):1082-1087.
8. GRESSNER O A,GRESSNER A M.Connective tissue growth factor:a fibrogenic master switch in fibrotic liver diseases[J].Liver Int,2008,28(8):1065-1079.
9. LI J H,HUANG X R,ZHU H J,et al.Role of TGF-beta signaling in extracellular matrix production under high glucose conditions[J].Kidney Int,2003,63(6):2010-2019.
10. 唐文,蒋明德,李小安.Sp600125对乙醛刺激的大鼠肝星状细胞凋亡及Fas蛋白表达的影响[J].西南国防医药,2008,18(3):334-336.
11. YAO J J,YU W L,CHANG Y,et al.Targeted screening of SiRNA directed HBV polymerase gene for effective inhibition of HBV expression[J].J Huazhong Univ Sci Technol,2008,28(3):266-271.
12. 沙龙泽,曾毅,王建博,等.小RNA分子研究进展[J].生物学通报,2009,44(1):1-5.

Journal of Biomedical Engineering

Effect of Tetramethylpyrazine and Rat CTGF miRNA Plasmids on Connective Tissue Growth Factor, Transforming Growth Factor-beta in High Glucose Stimulated Hepatic Stellate Cells

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