Progress and Prospect of Applications of Silk Fibroin in Construction of Tissue Engineering Scaffold_Journal of Biomedical Engineering

Authors：

YINLihua , WANGLin ,  YUZhanhai

Stomatological College of Lanzhou University, Lanzhou 730000, China;

Corresponding author：

YUZhanhai, Email: yuzhanhai@lzu.edu.cn

Keywords：

silk fibroin; scaffold construction; tissue engineering

DOI：

10.7507/1001-5515.20140086

Video：

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With the development of tissue engineering, a variety of forms of silk fibroin (SF) scaffolds has been applied to research of constructing variety of organization based on cells, which has become scientific focus in recent years. In this paper we introduced the source and structure of SF and the fabrication method of the scaffold, and also address the SF application progress in several relevant fields of tissue engineering, such as bone, cartilage, skin, blood vessel and nerves. Finally, we discuss the future leading prospect of the SF in order to provide reference for subsequent research.

Citation： YINLihua, WANGLin, YUZhanhai. Progress and Prospect of Applications of Silk Fibroin in Construction of Tissue Engineering Scaffold. Journal of Biomedical Engineering, 2014, 31(2): 467-471. doi: 10.7507/1001-5515.20140086 Copy

1.	LANGER R,VACANTI J P.Tissue engineering[J].Science,1993,260(5110):920-926.
2.	吴任东,杨辉,张磊,等.组织工程支架快速成形技术研究现状[J].机械工程学报,2011,47(5):170-176.
3.	OMENETTO F G,KAPLAN D L.New opportunities for an ancient material[J].Science,2010,329(5991):528-531.
4.	BITTENCOURT D,OLIVEIRA P F,PROSDOCIMI F,et al.Protein families,natural history and biotechnological aspects of spider silk[J].Genet Mol Res,2012,11(3):2360-2380.
5.	VEPARI C,KAPLAN D L.Silk as a biomaterial[J].Prog Polym Sci,2007,32(8-9):991-1007.
6.	WANG Y,KIM H J,VUNJAK-NOVAKOVIC G,et al.Stem cell-based tissue engineering with silk biomaterials[J].Biomaterials,2006,27(36):6064-6082.
7.	胡烨婷,王新刚,俞晓波,等.组织工程支架材料丝素蛋白研究进展[J].中华烧伤杂志,2011,27(4):291-294.
8.	GIL E S,PARK S H,MARCHANT J,et al.Response of human corneal fibroblasts on silk film surface patterns[J].Macromol Biosci,2010,10(6):664-673.
9.	ZHANG K H,YIN A L,HUANG C,et al.Degradation of electrospun SF/P (LLA-CL) blended nanofibrous scaffolds in vitro[J].Polym Degrad Stabil,2011,96(12):2266-2275.
10.	KUNDU S C,KUNDU B,TALUKDAR S,et al.Invited review nonmulberry silk biopolymers[J].Biopolymers,2012,97(6):455-467.
11.	LAWRENCE B D,WHARRAM S,KLUGE J A,et al.Effect of hydration on silk film material properties[J].Macromol Biosci,2010,10(4):393-403.
12.	CHI N H,YANG M C,CHUNG T W,et al.Cardiac repair achieved by bone marrow mesenchymal stem cells/silk fibroin/hyaluronic acid patches in a rat of myocardial infarction model[J].Biomaterials,2012,33(22):5541-5551.
13.	JHA B S,COLELLO R J,BOWMAN J R,et al.Two pole air gap electrospinning:fabrication of highly aligned,three-dimensional scaffolds for nerve reconstruction[J].Acta Biomater,2011,7(1):203-215.
14.	LI S,WU H,HU X D,et al.Preparation of electrospun PLGA-silk fibroin nanofibers-based nerve conduits and evaluation in vivo[J].Artif Cells Blood Substit Immobil Biotechnol,2012,40(1-2):171-178.
15.	CHO Y I,CHOI J S,JEONG S Y,et al.Nerve growth factor (NGF)-conjugated electrospun nanostructures with topographical cues for neuronal differentiation of mesenchymal stem cells[J].Acta Biomater,2010,6(12):4725-4733.
16.	LAWRENCE B D,MARCHANT J K,PINDRUS M A,et al.Silk film biomaterials for cornea tissue engineering[J].Biomaterials,2009,30(7):1299-1308.
17.	GUZIEWICZ N,BEST A,PEREZ-RAMIREZ B,et al.Lyophilized silk fibroinhydrogels for the sustained local delivery of therapeutic monoclonal antibodies[J].Biomaterials,2011,32(10):2642-2650.
18.	CHAO P H,YODMUANG S,WANG X,et al.Silk hydrogel for cartilage tissue engineering[J].J Biomed Mater Res B Appl Biomater,2010,95(1):84-90.
19.	DIAB T,PRITCHARD E M,UHRIG B A,et al.A silk hydrogel-based delivery system of bone morphogenetic protein for the treatment of large bone defects[J].J Mech Behav Biomed Mater,2012,11(11):123-131.
20.	HOFMANN S,HAGENMVLLER H,KOCH A M,et al.Control of in vitro tissue-engineered bone-like structures using human mesenchymal stem cells and porous silk scaffolds[J].Biomaterials,2007,28(6):1152-1162.
21.	MANDAL B B,GRINBERG A,GIL E S,et al.High-strength silk protein scaffolds for bone repair[J].Proc Natl Acad Sci U S A,2012,109(20):7699-7704.
22.	KINAHAN M E,FILIPPIDI E,KÖSTER S,et al.Tunable silk:using microfluidics to fabricate silk fibers with controllable properties[J].Biomacromolecules,2011,12(5):1504-1511.
23.	ZHAO Y,CHEN J,CHOU A H,et al.Nonwoven silk fibroin net/nano-hydroxyapatite scaffold:preparation and characterization[J].J Biomed Mater Res a,2009,91(4):1140-1149.
24.	WANG J,YE R,WEI Y,et al.The effects of electrospun TSF nanofiber diameter and alignment on neuronal differentiation of human embryonic stem cells[J].J Biomed Mater Res A,2012,100(3):632-645.
25.	江霞蓉,管娟,陈新,等.具有定向形貌的丝素蛋白支架的制备[J].化学学报,2010,68(18):1909-1914.
26.	CHO H J,KI C S,OH H,et al.Molecular weight distribution and solution properties of silk fibroins with different dissolution conditions[J].Int J Biol Macromol,2012,51(3):336-341.
27.	YANG M,SHUAI Y,HE W,et al.Preparation of porous scaffolds from silk fibroin extracted from the silk gland of bombyx mori (B.mori)[J].Int J Mol Sci,2012,13(6):7762-7775.
28.	RAJKHOWA R,GIL E S,KLUGE J,et al.Reinforcing silk scaffolds with silk particles[J].Macromol Biosci,2010,10(6):599-611.
29.	杨磊,朱良均,杨明英,等.丝素组织工程支架的研究进展[J].蚕业科学,2011,37(2):296-302.
30.	FISCHER R L,MCCOY M G,GRANT S A.Electrospinning collagen and hyaluronic acid nanofiber meshes[J].J Mater Sci Mater Med,2012,(7):1645-1654.
31.	ZHANG F,ZUO B Q,FAN Z H,et al.Mechanisms and control of silk-based electrospinning[J].Biomacromolecules,2012,13(3):798-804.
32.	ZHOU J,CAO C B,MA X L,et al.Electrospinning of silk fibroin and collagen for vascular tissue engineering[J].Int J Biol Macromol,2010,47(4):514-519.
33.	黄继伟,张锋,左保齐.静电纺丝素蛋白及其应用于组织工程的研究进展[J].天津工业大学学报,2011,30(1):16-21.
34.	吴小建,陈一心.蚕丝丝素蛋白在骨科领域的应用进展[J].临床骨科杂志,2011,14(2):214-217.
35.	MEINEL L,FAJARDO R,HOFMANN S,et al.Silk implants for the healing of critical size bone defects[J].Bone,2005,37(5):688-698.
36.	PARK S Y,KI C S,PARK Y H,et al.Electrospun silk fibroin scaffolds with macropores for bone regeneration:an in vitro and in vivo study[J].Tissue Eng Part A,2010,16(4):1271-1279.
37.	ROCKWOOD D N,GIL E S,PARK S H,et al.Ingrowth of human mesenchymal stem cells into porous silk particle reinforced silk composite scaffolds:an in vitro study[J].Acta Biomater,2011,7(1):144-151.
38.	CORREIA C,BHUMIRATANA S,YAN L P,et al.Development of silk-based scaffolds for tissue engineering of bone from human adipose-derived stem cells[J].Acta Biomater,2012,8(7):2483-2492.
39.	CHEON Y W,LEE W J,BAEK H S,et al.Enhanced chondrogenic responses of human articular chondrocytes onto silk fibroin/wool keratose scaffolds treated with microwave-induced argon plasma[J].Artif Organs,2010,34(5):384-392.
40.	KAMBE Y,YAMAMOTO K,KOJIMA K,et al.Effects of RGDS sequence genetically interfused in the silk fibroin light chain protein on chondrocyte adhesion and cartilage synthesis[J].Biomaterials,2010,31(29):7503-7511.
41.	JEONG L,YEO I S,KIM H N,et al.Plasma-treated silk fibroin nanofibers for skin regeneration[J].Int J Biol Macromol,2009,44(3):222-228.
42.	GUAN G,BAI L,ZUO B,et al.Promoted dermis healing from full-thickness skin defect by porous silk fibroin scaffolds (PSFSs)[J].Biomed Mater Eng,2010,20(5):295-308.
43.	BHARDWAJ N,CHAKRABORTY S,KUNDU S C.Freeze-gelled silk fibroin protein scaffolds for potential applications in soft tissue engineering[J].Int J Biol Macromol,2011,49(3):260-267.
44.	侯春春,张胡静,李圣春,等.蚕丝蛋白生物医学材料的研究现状[J].丝绸,2010,33(7):18-22.
45.	ZHANG K H,MO X M,HUANG C,et al.Electrospun scaffolds from silk fibroin and their cellular compatibility[J].J Biomed Mater Res a,2010,93(3):976-983.
46.	SOFFER L,WANG X Y,ZHANG X H,et al.Silk-based electrospun tubular scaffolds for tissue-engineered vascular grafts[J].J Biomater Sci Polym Ed,2008,19(5):653-664.
47.	TANG X,DING F,YANG Y,et al.Evaluation on in vitro biocompatibility of silk fibroin-based biomaterials with primarily cultured hippocampal neurons[J].J Biomed Mater Res A,2009,91(1):166-174.
48.	TANG X,XUE C,WANG Y,et al.Bridging peripheral nerve defects with a tissue engineered nerve graft composed of an in vitro cultured nerve equivalent and a silk fibroin-based scaffold[J].Biomaterials,2012,33(15):3860-3867.(上接第461页;Continued from Page 461)颅内感染患者颅内压的临床应用价值[J].临床神经病学杂志,2012,25(3):177-180.

1. LANGER R,VACANTI J P.Tissue engineering[J].Science,1993,260(5110):920-926.
2. 吴任东,杨辉,张磊,等.组织工程支架快速成形技术研究现状[J].机械工程学报,2011,47(5):170-176.
3. OMENETTO F G,KAPLAN D L.New opportunities for an ancient material[J].Science,2010,329(5991):528-531.
4. BITTENCOURT D,OLIVEIRA P F,PROSDOCIMI F,et al.Protein families,natural history and biotechnological aspects of spider silk[J].Genet Mol Res,2012,11(3):2360-2380.
5. VEPARI C,KAPLAN D L.Silk as a biomaterial[J].Prog Polym Sci,2007,32(8-9):991-1007.
6. WANG Y,KIM H J,VUNJAK-NOVAKOVIC G,et al.Stem cell-based tissue engineering with silk biomaterials[J].Biomaterials,2006,27(36):6064-6082.
7. 胡烨婷,王新刚,俞晓波,等.组织工程支架材料丝素蛋白研究进展[J].中华烧伤杂志,2011,27(4):291-294.
8. GIL E S,PARK S H,MARCHANT J,et al.Response of human corneal fibroblasts on silk film surface patterns[J].Macromol Biosci,2010,10(6):664-673.
9. ZHANG K H,YIN A L,HUANG C,et al.Degradation of electrospun SF/P (LLA-CL) blended nanofibrous scaffolds in vitro[J].Polym Degrad Stabil,2011,96(12):2266-2275.
10. KUNDU S C,KUNDU B,TALUKDAR S,et al.Invited review nonmulberry silk biopolymers[J].Biopolymers,2012,97(6):455-467.
11. LAWRENCE B D,WHARRAM S,KLUGE J A,et al.Effect of hydration on silk film material properties[J].Macromol Biosci,2010,10(4):393-403.
12. CHI N H,YANG M C,CHUNG T W,et al.Cardiac repair achieved by bone marrow mesenchymal stem cells/silk fibroin/hyaluronic acid patches in a rat of myocardial infarction model[J].Biomaterials,2012,33(22):5541-5551.
13. JHA B S,COLELLO R J,BOWMAN J R,et al.Two pole air gap electrospinning:fabrication of highly aligned,three-dimensional scaffolds for nerve reconstruction[J].Acta Biomater,2011,7(1):203-215.
14. LI S,WU H,HU X D,et al.Preparation of electrospun PLGA-silk fibroin nanofibers-based nerve conduits and evaluation in vivo[J].Artif Cells Blood Substit Immobil Biotechnol,2012,40(1-2):171-178.
15. CHO Y I,CHOI J S,JEONG S Y,et al.Nerve growth factor (NGF)-conjugated electrospun nanostructures with topographical cues for neuronal differentiation of mesenchymal stem cells[J].Acta Biomater,2010,6(12):4725-4733.
16. LAWRENCE B D,MARCHANT J K,PINDRUS M A,et al.Silk film biomaterials for cornea tissue engineering[J].Biomaterials,2009,30(7):1299-1308.
17. GUZIEWICZ N,BEST A,PEREZ-RAMIREZ B,et al.Lyophilized silk fibroinhydrogels for the sustained local delivery of therapeutic monoclonal antibodies[J].Biomaterials,2011,32(10):2642-2650.
18. CHAO P H,YODMUANG S,WANG X,et al.Silk hydrogel for cartilage tissue engineering[J].J Biomed Mater Res B Appl Biomater,2010,95(1):84-90.
19. DIAB T,PRITCHARD E M,UHRIG B A,et al.A silk hydrogel-based delivery system of bone morphogenetic protein for the treatment of large bone defects[J].J Mech Behav Biomed Mater,2012,11(11):123-131.
20. HOFMANN S,HAGENMVLLER H,KOCH A M,et al.Control of in vitro tissue-engineered bone-like structures using human mesenchymal stem cells and porous silk scaffolds[J].Biomaterials,2007,28(6):1152-1162.
21. MANDAL B B,GRINBERG A,GIL E S,et al.High-strength silk protein scaffolds for bone repair[J].Proc Natl Acad Sci U S A,2012,109(20):7699-7704.
22. KINAHAN M E,FILIPPIDI E,KÖSTER S,et al.Tunable silk:using microfluidics to fabricate silk fibers with controllable properties[J].Biomacromolecules,2011,12(5):1504-1511.
23. ZHAO Y,CHEN J,CHOU A H,et al.Nonwoven silk fibroin net/nano-hydroxyapatite scaffold:preparation and characterization[J].J Biomed Mater Res a,2009,91(4):1140-1149.
24. WANG J,YE R,WEI Y,et al.The effects of electrospun TSF nanofiber diameter and alignment on neuronal differentiation of human embryonic stem cells[J].J Biomed Mater Res A,2012,100(3):632-645.
25. 江霞蓉,管娟,陈新,等.具有定向形貌的丝素蛋白支架的制备[J].化学学报,2010,68(18):1909-1914.
26. CHO H J,KI C S,OH H,et al.Molecular weight distribution and solution properties of silk fibroins with different dissolution conditions[J].Int J Biol Macromol,2012,51(3):336-341.
27. YANG M,SHUAI Y,HE W,et al.Preparation of porous scaffolds from silk fibroin extracted from the silk gland of bombyx mori (B.mori)[J].Int J Mol Sci,2012,13(6):7762-7775.
28. RAJKHOWA R,GIL E S,KLUGE J,et al.Reinforcing silk scaffolds with silk particles[J].Macromol Biosci,2010,10(6):599-611.
29. 杨磊,朱良均,杨明英,等.丝素组织工程支架的研究进展[J].蚕业科学,2011,37(2):296-302.
30. FISCHER R L,MCCOY M G,GRANT S A.Electrospinning collagen and hyaluronic acid nanofiber meshes[J].J Mater Sci Mater Med,2012,(7):1645-1654.
31. ZHANG F,ZUO B Q,FAN Z H,et al.Mechanisms and control of silk-based electrospinning[J].Biomacromolecules,2012,13(3):798-804.
32. ZHOU J,CAO C B,MA X L,et al.Electrospinning of silk fibroin and collagen for vascular tissue engineering[J].Int J Biol Macromol,2010,47(4):514-519.
33. 黄继伟,张锋,左保齐.静电纺丝素蛋白及其应用于组织工程的研究进展[J].天津工业大学学报,2011,30(1):16-21.
34. 吴小建,陈一心.蚕丝丝素蛋白在骨科领域的应用进展[J].临床骨科杂志,2011,14(2):214-217.
35. MEINEL L,FAJARDO R,HOFMANN S,et al.Silk implants for the healing of critical size bone defects[J].Bone,2005,37(5):688-698.
36. PARK S Y,KI C S,PARK Y H,et al.Electrospun silk fibroin scaffolds with macropores for bone regeneration:an in vitro and in vivo study[J].Tissue Eng Part A,2010,16(4):1271-1279.
37. ROCKWOOD D N,GIL E S,PARK S H,et al.Ingrowth of human mesenchymal stem cells into porous silk particle reinforced silk composite scaffolds:an in vitro study[J].Acta Biomater,2011,7(1):144-151.
38. CORREIA C,BHUMIRATANA S,YAN L P,et al.Development of silk-based scaffolds for tissue engineering of bone from human adipose-derived stem cells[J].Acta Biomater,2012,8(7):2483-2492.
39. CHEON Y W,LEE W J,BAEK H S,et al.Enhanced chondrogenic responses of human articular chondrocytes onto silk fibroin/wool keratose scaffolds treated with microwave-induced argon plasma[J].Artif Organs,2010,34(5):384-392.
40. KAMBE Y,YAMAMOTO K,KOJIMA K,et al.Effects of RGDS sequence genetically interfused in the silk fibroin light chain protein on chondrocyte adhesion and cartilage synthesis[J].Biomaterials,2010,31(29):7503-7511.
41. JEONG L,YEO I S,KIM H N,et al.Plasma-treated silk fibroin nanofibers for skin regeneration[J].Int J Biol Macromol,2009,44(3):222-228.
42. GUAN G,BAI L,ZUO B,et al.Promoted dermis healing from full-thickness skin defect by porous silk fibroin scaffolds (PSFSs)[J].Biomed Mater Eng,2010,20(5):295-308.
43. BHARDWAJ N,CHAKRABORTY S,KUNDU S C.Freeze-gelled silk fibroin protein scaffolds for potential applications in soft tissue engineering[J].Int J Biol Macromol,2011,49(3):260-267.
44. 侯春春,张胡静,李圣春,等.蚕丝蛋白生物医学材料的研究现状[J].丝绸,2010,33(7):18-22.
45. ZHANG K H,MO X M,HUANG C,et al.Electrospun scaffolds from silk fibroin and their cellular compatibility[J].J Biomed Mater Res a,2010,93(3):976-983.
46. SOFFER L,WANG X Y,ZHANG X H,et al.Silk-based electrospun tubular scaffolds for tissue-engineered vascular grafts[J].J Biomater Sci Polym Ed,2008,19(5):653-664.
47. TANG X,DING F,YANG Y,et al.Evaluation on in vitro biocompatibility of silk fibroin-based biomaterials with primarily cultured hippocampal neurons[J].J Biomed Mater Res A,2009,91(1):166-174.
48. TANG X,XUE C,WANG Y,et al.Bridging peripheral nerve defects with a tissue engineered nerve graft composed of an in vitro cultured nerve equivalent and a silk fibroin-based scaffold[J].Biomaterials,2012,33(15):3860-3867.(上接第461页;Continued from Page 461)颅内感染患者颅内压的临床应用价值[J].临床神经病学杂志,2012,25(3):177-180.

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