Research on the Treatment of Liver Failure Rats with Transplantation of Alginate Microencapsulated Hepatocytes <i>In Vivo</i> Based on Poly-ornithine_Journal of Biomedical Engineering

Authors：

WANGJian , XULiming , TANGJinglong , WANGShuo ,  WANGChunren

National Institutes For Food and Drug Control, Beijing 100050, China;

Corresponding author：

WANGChunren, Email: churenwang@263.net

Keywords：

poly-ornithine; microcapsule; liver cell; liver; in vivo transplantation; tissue engineering

DOI：

10.7507/1001-5515.20140120

Video：

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This study aims to explore the effects of alginate-poly ornithine-alginate (A-PLO-A) and barium alginate-poly ornithine-alginate (B-PLO-A) microcapsules as cells carriers during implantation. Mice hepatocytes coated in A-PLO-A and B-PLO-A microcapsules were implanted into rats with acute liver failure caused by intraperitoneal injection of D-galactosamine. The rat survival rate, liver cell growth, proliferation and metabolism within the microcapsules were investigated, as well as its effect on the improvement of rat acute liver failure. The influence of A-PLO-A-free microcapsules, B-PLO-A-free microcapsules, isolated liver cells, A-PLO-A microcapsule-coated and B-PLO-A microcapsule-coated liver cells was studied. It was found that the chemical-free microcapsules showed no positive effect on the rats with liver failures, with a death rate of 100% in both groups 3 days after the implantation. The ALT, AST and ALB levels were all improved in the isolated liver cell group, the A-PLO-A microcapsule-coated and the B-PLO-A microcapsule-coated groups. The survival rate of both microcapsule-coated liver cell groups was significantly higher than that of the chemical-free microcapsule group and the isolated liver cells group. The microcapsules were retrieved after 4 weeks' implantation, which were observed to be smooth with no cells attaching to the surface. No apparent fibrosis was observed. This research demonstrated the physical stability and the biocompatibility of the PLO-based alginate microcapsules and therefore they could be used as liver cell carriers during implantation.

Citation： WANGJian, XULiming, TANGJinglong, WANGShuo, WANGChunren. Research on the Treatment of Liver Failure Rats with Transplantation of Alginate Microencapsulated Hepatocytes In Vivo Based on Poly-ornithine. Journal of Biomedical Engineering, 2014, 31(3): 642-647. doi: 10.7507/1001-5515.20140120 Copy

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1. KOBAYASHI T, AOMATSU Y, IWATA H, et al. Indefinite islet protection from autoimmune destruction in nonobese diabetic mice by agarose microencapsulation without immunosuppression[J]. Transplantation, 2003, 75(5):619-625.
2. LIU Z, CHANG T M. Effects of bone marrow cells on hepatocytes:when co-cultured or co-encapsulated together[J]. Artif Cells Blood Substit Immobil Biotechnol, 2000, 28(4):365-374.
3. DE VOS P, DE HAAN B, WOLTERS G H, et al. Factors influencing the adequacy of microencapsulation of rat pancreatic islets[J]. Transplantation, 1996, 62(7):888-893.
4. SAWHNEY A S, HUBBELL J A. Poly(ethylene oxide)-graft-poly(L-lysine) copolymers to enhance the biocompatibility of poly(L-lysine)-alginate microcapsule membranes[J]. Biomaterials, 1992, 13(12):863-870.
5. DE CASTRO M, ORIVE G, HERNÁNDEZ R M, et al. Comparative study of microcapsules elaborated with three polycations (PLL, PDL, PLO) for cell immobilization[J]. J Microencapsul, 2005, 22(3):303-315.
6. TAM S K, BILODEAU S, DUSSEAULT J, et al. Biocompatibility and physicochemical characteristics of alginate-polycation microcapsules[J]. Acta Biomater, 2011, 7(4):1683-1692.
7. HILLBERG A L, KATHIRGAMANATHAN K, LAM J B, et al. Improving alginate-poly-L-ornithine-alginate capsule biocompatibility through genipin crosslinking[J]. J Biomed Mater Res B Appl Biomater, 2013, 101(2):258-268.
8. MCQUILLING J P, ARENAS-HERRERA J, CHILDERS C, et al. New alginate microcapsule system for angiogenic protein delivery and immunoisolation of islets for transplantation in the rat omentum pouch[J]. Transplant Proc, 2011, 43(9):3262-3264.

Journal of Biomedical Engineering

Research on the Treatment of Liver Failure Rats with Transplantation of Alginate Microencapsulated Hepatocytes In Vivo Based on Poly-ornithine

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