Application of a New Design of Cryo-jaw and Its Biomechanical Evaluation in Rat Achilles Tendon <i>In Vitro</i>_Journal of Biomedical Engineering

Authors：

LIUErfu ¹ , SUNYanjun ¹ , PENGYongjin ² , XUWei ¹ , WANGYequan ^1,3 , XUKang ¹ , MOHANADKhalid Ahmed ¹ , LUYonggang ¹ ,  YANGLi ¹

1. Key Laboratory of Biorheological Science and Technology, Ministry of education & ‘111’ Project Laboratory of Biomechanics and Tissue Repair, Ministry of education, Bioengineering College, Chongqing University, Chongqing 400044, China;
2. Technical Centre of Dongfeng Auto Group Stock Co., Ltd., Wuhan 430058, China;
3. Institute of Forensic Science, Chongqing Public Security Bureau, Chongqing 400021, China;

Corresponding author：

YANGLi, Email: yanglibme@cqu.edu.cn

Keywords：

frozen water bath Cryo-jaw; achilles tendon; mechanical stretch test; application

DOI：

10.7507/1001-5515.20140126

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This study was aimed to design a new, accurate and easy-to-use water bath cryo-jaw, and try to solve the problems met in small animals achilles tendon mechanical testing. The muscle-tendon-bony units were fixed in the clamps. SD rats achilles tendon were randomly divided into group A and B. Group A was tested by the newly designed water bath cryo-jaw, while group B was treated by non-water bath cryo-jaw. The mechanical tests revealed that non of the samples of the newly-designed water bath cryo-jaw in group A slipped and fell off, and the achilles tendons were in a physiologically active state, but one of the group B samples slipped and fell off, and the others had the frozen phenomenon obviously. The maximum stress, fracture displacement and Young's modulus of the rats in group A were significantly different compared to those in group B (P<0.05). In conclusion, the new water bath cryo-jaw has more advantages than traditional ones. It exhibits a good simulation in vivo in the environmental conditions for testing the mechanical properties of the achilles tendon.

Citation： LIUErfu, SUNYanjun, PENGYongjin, XUWei, WANGYequan, XUKang, MOHANADKhalid Ahmed, LUYonggang, YANGLi. Application of a New Design of Cryo-jaw and Its Biomechanical Evaluation in Rat Achilles Tendon In Vitro. Journal of Biomedical Engineering, 2014, 31(3): 678-681. doi: 10.7507/1001-5515.20140126 Copy

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1. CARPENTER J E, HANKENSON K D. Animal models of tendon and ligament injuries for tissue engineering applications[J]. Biomaterials, 2004, 25(9):1715-1722.
2. CARPENTER J E, THOMOPOULOS S, SOSLOWSKY L J. Animal models of tendon and ligament injuries for tissue engineering applications[J]. Clin Orthop Relat Res, 1999, 367(Suppl):S296-S311.
3. CHEN X, SONG X H, YIN Z, et al. Stepwise differentiation of human embryonic stem cells promotes tendon regeneration by secreting fetal tendon matrix and differentiation factors[J]. Stem Cells, 2009, 27(6):1276-1287.
4. SHEN W, CHEN X, CHEN J, et al. The effect of incorporation of exogenous stromal cell-derived factor-1 alpha within a knitted silk-collagen sponge scaffold on tendon regeneration[J]. Biomaterials, 2010, 31(28):7239-7249.
5. HALDER A, ZOBITZ M E, SCHULTZ F, et al. Mechanical properties of the posterior rotator cuff[J]. Clin Biomech (Bristol, Avon), 2000, 15(6):456-462.
6. SODEN P D, KERSHAW I. Tensile testing of connective tissues[J]. Med Biol Eng, 1974, 12(4):510-518.
7. MATTHEWS G L, KEEGAN K G, GRAHAM H L. Effects of tendon grip technique (frozen versus unfrozen) on in vitro surface strain measurements of the equine deep digital flexor tendon[J]. Am J Vet Res, 1996, 57(1):111-115.
8. RIEMERSA D J, SCHAMHARDT H C. The cryo-jaw, a clamp designed for in vitro rheology studies of horse digital flexor tendons[J]. J Biomech, 1982, 15(8):619-620.
9. WIELOCH P, BUCHMANN G, ROTH W, et al. A cryo-jaw designed for in vitro tensile testing of the healing achilles tendons in rats[J]. J Biomech, 2004, 37(11):1719-1722.
10. SHARKEY N A, SMITH T S, LUNDMARK D C. Freeze clamping musculo-tendinous junctions for in vitro simulation of joint mechanics[J]. J Biomech, 1995, 28(5):631-635.

Journal of Biomedical Engineering

Application of a New Design of Cryo-jaw and Its Biomechanical Evaluation in Rat Achilles Tendon In Vitro

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