Properties and Biocompatibility of Collagen Scaffold Modified by Genipin Cross-linked L-lysine_Journal of Biomedical Engineering

Authors：

JIANGDonglin ¹ , YANGJunyu ² , JIANGShengyang ¹ , LUGuozhong ¹ ,  ZHAOPeng ¹

1. Centre Laboratory, the Third Affiliated Hospital of Nantong University, Wuxi 214041, China;
2. Pharmacy College, Jiangnan University, Wuxi 214122, China;

Corresponding author：

ZHAOPeng, Email: lausee@126.com

Keywords：

collagen; L-lysine; genipin; tissue engineering

DOI：

10.7507/1001-5515.20140153

Video：

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Collagen (Coll), as the basic material of matrix scaffolds for cell growth, has been widely used in the field of tissue engineering and regenerative medicine. In this study, collagen protein was modified by L-lysine (Lys), and cross-linked by genipin (GN) to prepare the L-lysine-modified collagen (Lys-Coll-GN) scaffolds. Microstructure, pore size, porosity, stability and biocompatibility of Lys-Coll-GN scaffolds were observed. The results showed that the bond between L-lysine and collagen protein molecule was formed by generating amide linkage, and mouse embryo fibroblasts proliferation was not inhibited in the Lys-Coll-GN scaffolds. In the multiple comparisons of Coll-scaffolds, Coll-GNscaffolds and Lys-Coll-GN-scaffolds, Coll-scaffolds was the worst in mechanical characteristics while the highest in biodegradation rate. Compared to Coll-GN scaffolds, Lys-Coll-GN scaffolds had more fiber structure, higher interval porosity (P<0.01). Although the tensile stress of Lys-Coll-GN scaffolds reduced significantly, its elongation length extended when the scaffolds was fractured (P<0.01). The percentage of Lys-Coll-GN scaffolds residual weight was lower than that of Coll-GN-scaffolds after all the scaffolds were treated by collagenase for 5 days (P<0.01).This study suggested that Lys-Coll-GN scaffold had good biocompatibility, and it improved the mechanical property and degradation velocity for collagen-based scaffold. This study gave a new predominant type of tissue engineering scaffold for the regenerative medicine.

Citation： JIANGDonglin, YANGJunyu, JIANGShengyang, LUGuozhong, ZHAOPeng. Properties and Biocompatibility of Collagen Scaffold Modified by Genipin Cross-linked L-lysine. Journal of Biomedical Engineering, 2014, 31(4): 816-821. doi: 10.7507/1001-5515.20140153 Copy

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1. DONZELLI E, SALVADÈ A, MIMO P, et al. Mesenchymal stem cells cultured on a collagen scaffold: in vitro osteogenic differentiation[J]. Arch Oral Biol, 2007, 52(1): 64-73.
2. SHEN Y H, SHOICHET M S, RADISIC M. Vascular endothelial growth factor immobilized in collagen scaffold promotes penetration and proliferation of endothelial cells[J]. Acta Biomater, 2008, 4(3): 477-489.
3. ZHONG S, TEO W E, ZHU X, et al. An aligned nanofibrous collagen scaffold by electrospinning and its effects on in vitro fibroblast culture[J]. J Biomed Mater Res A, 2006, 79(3): 456-463.
4. KIYOZUMI T, KANATANI Y, ISHIHARA M, et al. The effect of chitosan hydrogel containing DMEM/F12 medium on full-thickness skin defects after deep dermal burn[J]. Burns, 2007, 33(5): 642-648.
5. JAN J S, CHEN P S, HSIEH P L, et al. Silicification of genipin-cross-linked polypeptide hydrogels toward biohybrid materials and mesoporous oxides[J]. ACS Appl Mater Interfaces, 2012, 4(12): 6865-6874.
6. 杨辉,杨丹凤,张华山,等.4种典型纳米材料对小鼠胚胎成纤维细胞毒性的初步研究[J].生态毒理学报,2007,2(4):427-434.
7. YAMAUCHI M, SRICHOLPECH M. Lysine post-translational modifications of collagen[J]. Essays Biochem, 2012, 52: 113-133.
8. HE Q F, ZHAO Y N, CHEN B, et al. Improved cellularization and angiogenesis using collagen scaffolds chemically conjugated with vascular endothelial growth factor[J]. Acta Biomater, 2011, 7(3): 1084-1093.
9. CALIARI S R, WEISGERBER D W, RAMIREZ M A, et al. The influence of collagen-glycosaminoglycan scaffold relative density and microstructural anisotropy on tenocyte bioactivity and transcriptomic stability[J]. J Mech Behav Biomed Mater, 2012, 11(11): 27-40.
10. KEMPF M, MIYAMURA Y, LIU P Y, et al. A denatured collagen microfiber scaffold seeded with human fibroblasts and keratinocytes for skin grafting[J]. Biomaterials, 2011, 32(21): 4782-4792.
11. 张弩,陈廖斌.复合基质细胞衍生因子-1β、转化生长因子-β1的新型温敏性壳聚糖水凝胶的制备及其缓释性能[J].中华实验外科杂志,2010,27(9):1320-1322.
12. MA W, TANG C H, YIN S W, et al. Genipin-crosslinked gelatin films as controlled releasing carriers of lysozyme[J]. Food Res Int, 2013, 51(1): 321-324.
13. POWELL H M, BOYCE S T. EDC cross-linking improves skin substitute strength and stability[J]. Biomaterials, 2006, 27(34): 5821-5827.
14. MA L, GAO C, MAO Z, et al. Biodegradability and cell-mediated contraction of porous collagen scaffolds: the effect of lysine as a novel crosslinking bridge[J]. J Biomed Mater Res A, 2004, 71(2): 334-342.
15. 缪雪华,周勇,任伟业,等.胶原海绵促进肉芽生长与创面愈合实验研究[J].中国当代医药,2012,19(10):26-27, 30.
16. 刘艳明,张端莲.胶原海绵膜修复烧伤皮肤创面的实验研究[J].数理医药学杂志,2011,24(3):279-281.
17. 陈英华,董为人,陈清元,等.人发角蛋白-胶原海绵-聚甲基丙烯酸羟乙酯复合生物敷料对大鼠烧伤创面修复作用的实验研究[J].南方医科大学学报,2007,27(11):1621-1626.
18. 佟刚,雷静,钟翠红,等.不同pH与蛋白含量的胶原海绵植入缺损骨组织部位的疗效及安全性考察[J].生物医学工程学杂志,2012,29(6):1125-1130.
19. 周庆翰,林娟.天然氨基酸生物材料的自组装与细胞相容性研究[J].生物医学工程学杂志,2012,29(5):898-902.

Journal of Biomedical Engineering

Properties and Biocompatibility of Collagen Scaffold Modified by Genipin Cross-linked L-lysine

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