Effect of Basic Fibroblast Growth Factor on Endogenous Neural Stem Cell in Rat Cerebral Cortex with Global Cerebral Ischemia-reperfusion_Journal of Biomedical Engineering

Authors：

RENMingxin ¹ , DENGXiaohui ¹ , GUOYiwei ¹ , ZHENGFengjin ² ,  FENGZhibo ¹

1. Department of Anatomy, Xinxiang Medical University, Xinxiang 453003, China;
2. West China School of Preclinical and Forensic Medicine, Sichuan University, Chengdu 610041, China;

Corresponding author：

FENGZhibo, Email: wodeshangmao2012@163.com

Keywords：

global cerebral ischemia; endogenous neural stem cell; basic fibroblast growth factor; rat

DOI：

10.7507/1001-5515.20140159

Video：

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The present paper is aimed to investigate the effect of basic fibroblast growth factor (bFGF) on proliferation, migration and differentiation of endogenous neural stem cell in rat cerebral cortex with global brain ischemia-reperfusion. A global brain ischemia-reperfusion model was established. Immunohistochemistry was used to observe the pathological changes and the expression of BrdU and Nestin in cerebral cortex. RT-PCR was used to measure the NSE mRNA in brain tissue. The results of measurements indicated that in sham operation group, there was no positive cell in cerebral cortex, and the content of NSE mRNA did not change. In the operation group, the expression of BrdU and Nestin increased significantly at the end of the 3rd day, and peaked on the 7th day. NSE mRNA expression did not significantly increase. In bFGF group, compared with sham operation group and model group, the number of BrdU-positive and Nestin-positive cells increased significantly at each time point (P<0.05), and peaked at the end of the 11th day, and the content of NSE mRNA increased significantly (P<0.05). This research demonstrated that the proliferation of endogenous neural stem cells in situ could be induced by global cerebral ischemia and reperfusion, and could be promoted and extended by bFGF. In additiion, bFGF might promote endogenous neural stem cells differentiated into neurons.

Citation： RENMingxin, DENGXiaohui, GUOYiwei, ZHENGFengjin, FENGZhibo. Effect of Basic Fibroblast Growth Factor on Endogenous Neural Stem Cell in Rat Cerebral Cortex with Global Cerebral Ischemia-reperfusion. Journal of Biomedical Engineering, 2014, 31(4): 846-849. doi: 10.7507/1001-5515.20140159 Copy

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5.	TZENG S F, WU J P. Responses of microglia and neural progenitors to mechanical brain injury[J]. Neuroreport, 1999, 10(11): 2287-2292.
6.	WANG P H, SCHWINDT T T, BARNABÉ G F, et al. Administration of neural precursor cells ameliorates renal ischemia-reperfusion injury[J]. Nephron Exp Nephrol, 2009, 112(1): e20-e28.
7.	TAKAGI Y, NOZAKI K, TAKAHASHI J, et al. Proliferation of neuronal precursor cells in the dentate gyrus is accelerated after transient forebrain ischemia in mice[J]. Brain Res, 1999, 831(1-2): 283-287.
8.	CHOI K C, YOO D S, CHO K S, et al. Effect of single growth factor and growth factor combinations on differentiation of neural stem cells[J]. J Korean Neurosurg Soc, 2008, 44(6): 375-381.
9.	TONG L, JI L, WANG Z, et al. Differentiation of neural stem cells into Schwann-like cells in vitro[J]. Biochem Biophys Res Commun, 2010, 401(4): 592-597.
10.	LASKOWSKI A, SCHMIDT W, DINKEL K, et al. bFGF and EGF modulate trauma-induced proliferation and neurogenesis in juvenile organotypic hippocampal slice cultures[J]. Brain Res, 2005, 1037(1-2): 78-89.
11.	QIAN X, DAVIS A A, GODERIE S K, et al. FGF2 concentration regulates the generation of neurons and glia from multipotent cortical stem cells[J]. Neuron, 1997, 18(1): 81-93.

1. HAGG T. From neurotransmitters to neurotrophic factors to neurogenesis[J]. Neuroscientist, 2009, 15(1): 20-27.
2. NOUREDDINI M, VERDI J, MORTAZAVI-TABATABAEI S A, et al. Human endometrial stem cell neurogenesis in response to NGF and bFGF[J]. Cell Biol Int, 2012, 36(10): 961-966.
3. GALLI R, GRITTI A, BONFANTI L, et al. Neural stem cells:an overview[J]. Circ Res, 2003, 92(6): 598-608.
4. 张兰兰,曹琼,胡子有,等.槲皮素对成年大鼠局灶性脑缺血后侧脑室室管膜下区神经干细胞增殖的影响[J].南方医科大学学报,2011,31(7):1200-1203.
5. TZENG S F, WU J P. Responses of microglia and neural progenitors to mechanical brain injury[J]. Neuroreport, 1999, 10(11): 2287-2292.
6. WANG P H, SCHWINDT T T, BARNABÉ G F, et al. Administration of neural precursor cells ameliorates renal ischemia-reperfusion injury[J]. Nephron Exp Nephrol, 2009, 112(1): e20-e28.
7. TAKAGI Y, NOZAKI K, TAKAHASHI J, et al. Proliferation of neuronal precursor cells in the dentate gyrus is accelerated after transient forebrain ischemia in mice[J]. Brain Res, 1999, 831(1-2): 283-287.
8. CHOI K C, YOO D S, CHO K S, et al. Effect of single growth factor and growth factor combinations on differentiation of neural stem cells[J]. J Korean Neurosurg Soc, 2008, 44(6): 375-381.
9. TONG L, JI L, WANG Z, et al. Differentiation of neural stem cells into Schwann-like cells in vitro[J]. Biochem Biophys Res Commun, 2010, 401(4): 592-597.
10. LASKOWSKI A, SCHMIDT W, DINKEL K, et al. bFGF and EGF modulate trauma-induced proliferation and neurogenesis in juvenile organotypic hippocampal slice cultures[J]. Brain Res, 2005, 1037(1-2): 78-89.
11. QIAN X, DAVIS A A, GODERIE S K, et al. FGF2 concentration regulates the generation of neurons and glia from multipotent cortical stem cells[J]. Neuron, 1997, 18(1): 81-93.

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Effect of Basic Fibroblast Growth Factor on Endogenous Neural Stem Cell in Rat Cerebral Cortex with Global Cerebral Ischemia-reperfusion

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