Comparison of Cell Elasticity Analysis Methods Based on Atomic Force Microscopy Indentation_Journal of Biomedical Engineering

Authors：

WANGZhe , HAOFengtao , CHENXiaohu , YANGZhouqi , DINGChong ,  SHANGPeng

Key laboratory for Space Biosciences and Biotechnology, Institute of Special Environmental Biophysics, School of Life Sciences, Northwestern Polytechnical University, Xi'an 710072, China;

Corresponding author：

SHANGPeng, Email: shangpeng@nwpu.edu.cn

Keywords：

atomic force microscopy; Young's modulus; contact point; force-distance curve

DOI：

10.7507/1001-5515.20140202

Video：

Export PDF Favorites Scan Get Citation

Abstract Full text Figures/Tables Video References Cited by

In order to investigate in greater detail the two methods based on Hertz model for analyzing force-distance curve obtained by atomic force microscopy, we acquired the force-distance curves of Hela and MCF-7 cells by atomic force microscopy (AFM) indentation in this study. After the determination of contact point, Young's modulus in different indentation depth were calculated with two analysis methods of "two point" and "slope fitting". The results showed that the Young's modulus of Hela cell was higher than that of MCF-7 cell,which is in accordance with the F-actin distribution of the two types of cell. We found that the Young's modulus of the cells was decreased with increasing indentation depth and the curve trends by "slope fitting". This indicated that the "slope fitting" method could reduce the error caused by the miscalculation of contact point. The purpose of this study was to provide a guidance for researcher to choose an appropriate method for analyzing AFM indentation force-distance curve.

Citation： WANGZhe, HAOFengtao, CHENXiaohu, YANGZhouqi, DINGChong, SHANGPeng. Comparison of Cell Elasticity Analysis Methods Based on Atomic Force Microscopy Indentation. Journal of Biomedical Engineering, 2014, 31(5): 1075-1079. doi: 10.7507/1001-5515.20140202 Copy

1.	CHAHINE N O, BLANCHETTE C, THOMAS C B, et al. Effect of age and cytoskeletal elements on the indentation-dependent mechanical properties of chondrocytes[J]. PLoS One, 2013, 8(4): e61651.
2.	CASUSO I, RICO F, SCHEURING S. Biological AFM: where we come from--where we are--where we may go[J]. J Mol Recognit, 2011, 24(3): 406-413.
3.	梁志红,周长忍.原子力显微镜在组织工程学中的应用[J].生物医学工程学杂志,2009,26(1):199-201.
4.	LEE G Y, LIM C T. Biomechanics approaches to studying human diseases[J]. Trends Biotechnol, 2007, 25(3): 111-118.
5.	DI CARLO D. A mechanical biomarker of cell state in medicine[J]. J Lab Autom, 2012, 17(1): 32-42.
6.	GUO Q, PARK S, MA H. Microfluidic micropipette aspiration for measuring the deformability of single cells[J]. Lab Chip, 2012, 12(15): 2687-2695.
7.	LAFRATTA C N. Optical tweezers for medical diagnostics[J]. Anal Bioanal Chem, 2013, 405(17): 5671-5677.
8.	KEE Y S, ROBINSON D N. Micropipette aspiration for studying cellular mechanosensory responses and mechanics[M]//EICHINGER L, RIVERO L. Dictyostelium discoideum Protocols. New York: Springer, 2013, 983:367-382.
9.	HERTZ H. Vber die Berührung fester elastischer Kürper[J]. J für die reine u angew Math, 1882, 1882(92):156-171.
10.	LIM C T, ZHOU E H, QUEK S T. Mechanical models for living cells--a review[J]. J Biomech, 2006, 39(2): 195-216.
11.	CARL P, SCHILLERS H. Elasticity measurement of living cells with an atomic force microscope: data acquisition and processing[J]. Pflugers Arch, 2008, 457(2): 551-559.
12.	CRICK S L, YIN F C. Assessing micromechanical properties of cells with atomic force microscopy: importance of the contact point[J]. Biomech Model Mechanobiol, 2007, 6(3): 199-210.
13.	张维,骞爱荣,高翔,等.细胞骨架染色中不同固定方法的探讨[J].中国细胞生物学学报,2010,32(1):149-153.
14.	鲁卓阳,龙建纲,李涤尘,等.基于原子力显微镜的细胞弹性测量及分析方法[J].电子显微学报,2012,31(4):339-345.
15.	LI Q S, LEE G Y, ONG C N, et al. AFM indentation study of breast cancer cells[J]. Biochem Biophys Res Commun, 2008, 374(4): 609-613.
16.	LEPORATTI S, VERGARA D, ZACHEO A, et al. Cytomechanical and topological investigation of MCF-7 cells by scanning force microscopy[J]. Nanotechnology, 2009, 20(5): 55103.
17.	TAKAI E, COSTA K D, SHAHEEN A, et al. Osteoblast elastic modulus measured by atomic force microscopy is substrate dependent[J]. Ann Biomed Eng, 2005, 33(7): 963-971.

1. CHAHINE N O, BLANCHETTE C, THOMAS C B, et al. Effect of age and cytoskeletal elements on the indentation-dependent mechanical properties of chondrocytes[J]. PLoS One, 2013, 8(4): e61651.
2. CASUSO I, RICO F, SCHEURING S. Biological AFM: where we come from--where we are--where we may go[J]. J Mol Recognit, 2011, 24(3): 406-413.
3. 梁志红,周长忍.原子力显微镜在组织工程学中的应用[J].生物医学工程学杂志,2009,26(1):199-201.
4. LEE G Y, LIM C T. Biomechanics approaches to studying human diseases[J]. Trends Biotechnol, 2007, 25(3): 111-118.
5. DI CARLO D. A mechanical biomarker of cell state in medicine[J]. J Lab Autom, 2012, 17(1): 32-42.
6. GUO Q, PARK S, MA H. Microfluidic micropipette aspiration for measuring the deformability of single cells[J]. Lab Chip, 2012, 12(15): 2687-2695.
7. LAFRATTA C N. Optical tweezers for medical diagnostics[J]. Anal Bioanal Chem, 2013, 405(17): 5671-5677.
8. KEE Y S, ROBINSON D N. Micropipette aspiration for studying cellular mechanosensory responses and mechanics[M]//EICHINGER L, RIVERO L. Dictyostelium discoideum Protocols. New York: Springer, 2013, 983:367-382.
9. HERTZ H. Vber die Berührung fester elastischer Kürper[J]. J für die reine u angew Math, 1882, 1882(92):156-171.
10. LIM C T, ZHOU E H, QUEK S T. Mechanical models for living cells--a review[J]. J Biomech, 2006, 39(2): 195-216.
11. CARL P, SCHILLERS H. Elasticity measurement of living cells with an atomic force microscope: data acquisition and processing[J]. Pflugers Arch, 2008, 457(2): 551-559.
12. CRICK S L, YIN F C. Assessing micromechanical properties of cells with atomic force microscopy: importance of the contact point[J]. Biomech Model Mechanobiol, 2007, 6(3): 199-210.
13. 张维,骞爱荣,高翔,等.细胞骨架染色中不同固定方法的探讨[J].中国细胞生物学学报,2010,32(1):149-153.
14. 鲁卓阳,龙建纲,李涤尘,等.基于原子力显微镜的细胞弹性测量及分析方法[J].电子显微学报,2012,31(4):339-345.
15. LI Q S, LEE G Y, ONG C N, et al. AFM indentation study of breast cancer cells[J]. Biochem Biophys Res Commun, 2008, 374(4): 609-613.
16. LEPORATTI S, VERGARA D, ZACHEO A, et al. Cytomechanical and topological investigation of MCF-7 cells by scanning force microscopy[J]. Nanotechnology, 2009, 20(5): 55103.
17. TAKAI E, COSTA K D, SHAHEEN A, et al. Osteoblast elastic modulus measured by atomic force microscopy is substrate dependent[J]. Ann Biomed Eng, 2005, 33(7): 963-971.

Previous Article
Experimental Study on Electrical Impedance Properties of Human Hepatoma Cells
Next Article
Study of TGF-β/Smad3 Signal Pathway Using the Technology of Flurorescence Resonance Energy Transfer

Journal of Biomedical Engineering

Comparison of Cell Elasticity Analysis Methods Based on Atomic Force Microscopy Indentation

Abstract Full text Figures/Tables Video References Cited by

Previous Article

Next Article

Format

Content