Mechanical Properties and Biological Evaluation of Buffalo Horn Material_Journal of Biomedical Engineering

Authors：

ZHANGQuanbin ¹ , ZHOUQunfei ² , SHANGuanghua ³ , CAOPing ⁴ , HUANGYaoxiong ¹ ,  AONingjian ¹

1. Department of Biomedical Engineering, Jinan University, Guangzhou 510632, China;
2. Department of Biotechnology, Jinan University, Guangzhou 510632, China;
3. The First Affiliated Hospital of Jinan University, Guangzhou 510632, China;
4. Shenzhen Testing Center of Medical Devices, Shenzhen 518057, China;

Corresponding author：

AONingjian, Email: taonj@jnu.edu.cn

Keywords：

buffalo horn material; mechanical property; microstructure; biological evaluation

DOI：

10.7507/1001-5515.20140246

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Mechanical properties and biological evaluation of buffalo horn material were examined in this study. The effects of sampling position of buffalo horn on mechanical properties were investigated with uniaxial tension and micron indentation tests. Meanwhile, the variation of element contents in different parts of buffalo horn was determined with elemental analysis, and the microstructure of the horn was measured with scanning electron microscopy. In addition, biological evaluation of buffalo horn was studied with hemolytic test, erythrocyte morphology, platelet and erythrocyte count, and implantation into mouse. Results showed that the buffalo horn had good mechanical properties and mechanical characteristic values of it gradually increased along with the growth direction of the horn, which may be closely related to its microstructure and element content of C, N, and S in different parts of the buffalo horn. On the other hand, because the buffalo horn does not have toxicity, it therefore does not cause hemolysis of erythrocyte and has a good affinity with it. Buffalo horn has good histocompatibility but meanwhile it may induce the platelet adhesion and aggregation. Even so, it does not continue to rise to induce a large number of platelet to aggregate with resulting blood clotting. Therefore, the buffalo horn material has been proved to possess good blood compatibility according to the preliminary evaluation.

Citation： ZHANGQuanbin, ZHOUQunfei, SHANGuanghua, CAOPing, HUANGYaoxiong, AONingjian. Mechanical Properties and Biological Evaluation of Buffalo Horn Material. Journal of Biomedical Engineering, 2014, 31(6): 1298-1304. doi: 10.7507/1001-5515.20140246 Copy

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1. WEGST U G K, ASHBY M F. The mechanical efficiency of natural materials[J]. Philosoph Mag, 2004, 84(21):2167-2186.
2. MARTELLI S M, MOORE G, PAES S S, et al. Influence of plasticizers on the water sorption isotherms and water vapor permeability of chicken feather keratin films[J]. LWT-Food Sci Technol, 2006, 39(3):292-301.
3. MOORE G R P, MARTELLI S M, GANDOLFO C, et al. Influence of the glycerol concentration on some physical properties of feather keratin films[J]. Food Hydrocol, 2006, 20(7):975-982.
4. MEYERS M A, CHEN P Y, LIN A Y M, et al. Biological materials:structure and mechanical properties[J]. Prog Mater Sci, 2008, 53(1):1-206.
5. FUDGE D S, GOSLINE J M. Molecular design of the alpha-keratin composite:insights from a matrix-free model, hagfish slime threads[J]. Proc R Soc Lond B, 2004, 271(1536):291-299.
6. DOUGLAS J E, MITTAL C, THOMASON J J, et al. The modulus of elasticity of equine hoof wall:implications for the mechanical function of the hoof[J]. J Exp Biol, 1996, 199(Pt 8):1829-1836.
7. COLLINS S N, COPE B C, HOPEGOOD R L, et al. Stiffness as a function of moisture content in natural materials:characterisation of hoof horn samples[J]. J Mater Sci, 1998, 33(21):5185-5191.
8. FRANCK A, COCQUYT G, SIMOENS P, et al. Biomechanical properties of bovine claw horn[J]. Biosyst Eng, 2006, 93(4):459-467.
9. CAMERON G J, WESS T J, BONSER R H C. Young's modulus varies with differential orientation of keratin in feathers[J]. J Struct Biol, 2003, 143(2):118-123.
10. WEI G, BHUSHAN B, TORGERSON P M. Nanomechanical characterization of human hair using nanoindentation and SEM[J]. Ultramicroscopy, 2005, 105(1-4):248-266.
11. FEUGHELMAN M. Mechanical properties and structure of alpha-keratin fibers:Wool, human hair and related fibres[M]. Sydney:University of New South Wales, 1997.
12. KITCHENER A, VINCENT J V. Composite theory and the effect of water on the stiffness of horn keratin[J]. J Mater Sci, 1987, 22(4):1385-1389.
13. KITCHENER A. An analysis of the forces of fighting of the blackbuck (Antilope cervicapra) and the bighorn sheep (Ovis canadensis) and the mechanical design of the horn of bovids[J]. J Zool, 1988, 214(1):1-20.
14. 徐新建.天然牛角材料组织相容性的初步研究[D].石家庄:河北医科大学,2010.
15. TOMBOLATO L, NOVITSKAYA E E, CHEN P Y, et al. Microstructure, elastic properties and deformation mechanisms of horn keratin[J]. Acta Biomater, 2010, 6(2):319-330.
16. SARIKAYA M, GUNNISON K E, YASREBI M, et al. Mechanical property——microstructural relationships in abalone shell[C]. Materials Research Society Symposia Proceedings. Cambridge:Cambridge University Press, 1989, 174:109-166.
17. AIZENBERG J, WEAVER J C, THANAWALA M S, et al. Skeleton of Euplectella sp.:structural hierarchy from the nanoscale to the macroscale[J]. Science, 2005, 309(5732):275-278.
18. SARIKAYA M, FONG H, SUNDERLAND N, et al. Biomimetic model of a sponge-spicular optical fiber-mechanical properties and structure[J]. J Mater Res, 2001, 16(5):1420-1428.
19. 郑书家.抗凝血生物材料的血液相容性研究[D].重庆:重庆大学,2004.
20. 韩俊艳,檀德宏,王敏伟.水牛角水解物的止血作用研究[J].实用药物与临床,2004,7(3):17-18.

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