Effects of Madder on Bone Biomechanical Property in Rats_Journal of Biomedical Engineering

Authors：

WUChenchen , YANGXiaowen , WANGWenlong , WANGShanshan , CAODandan , MAFeng , WANGJianguo , LUHao ,  ZHAOBaoyu

College of Veterinary Medicine, Northwest A & F University, Yangling 712100, China;

Corresponding author：

ZHAOBaoyu, Email: zhaobaoyu12005@163.com

Keywords：

madder; rats; bone biomechanics; femur

DOI：

10.7507/1001-5515.20150020

Video：

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Abstract Full text Figures/Tables Video References Cited by

Bones are stained into red color with feeding madder, but we do not know whether the fed madder can change the bone biomechanical properties and bone mineral contents in animals. In this research, we established a rat model with feeding madder. The bone biomechanical properties were detected by universal material mechanics, bone mineral contents were detected by inductively coupled plasma mass spectrometry and spectrometer, and red color material in bone was analyzed by high performance liquid chromatography. The results showed that bone biomechanical parameters in femur diaphysis in the 10% and 15% group rats were significantly higher than those in the control group after feeding madder for 6 months. The level of calcium, magnesium and zinc in femur diaphysis in 10% and 15% group rats were higher than those in the control group after feeding madder for 6 months. However, it was shown that the kidney congestion andhyperemia and the level of blood urea nitrogen and creatinine in the 15% group rats were significantly different compared to those in the control group rats after feeding madder for 6 months. The red colored material in bone is related to alizarin analyzed with high-performance liquid chromatography. The conclusion could be drawn that feeding 10% madder in diet was not toxic to the rats fed for 6 months, and it could improve bone biomechanical properties and increase bone mineral elements.

Citation： WUChenchen, YANGXiaowen, WANGWenlong, WANGShanshan, CAODandan, MAFeng, WANGJianguo, LUHao, ZHAOBaoyu. Effects of Madder on Bone Biomechanical Property in Rats. Journal of Biomedical Engineering, 2015, 32(1): 110-115. doi: 10.7507/1001-5515.20150020 Copy

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1. WU C C, LI X B, HAN T S, et al. Dietary pseudopurpurin improves bone geometry architecture and metabolism in red-bone Guishan goats[J]. PLoS One, 2012, 7(5): e37469.
2. NAKANISHI F, NAGASAWA Y, KABAYA Y, et al. Characterization of lucidin formation in Rubia tinctorum L[J]. Plant Physiol Biochem, 2005, 43(10-11):921-928.
3. YIN L, VENKATESAN S, KALYANASUNDARAM S, et al. Effect of microstructure on micromechanical performance of dry cortical bone tissues[J]. Mater Charact, 2009, 60(12): 1424-1431.
4. HAO Y J, ZHANG G, WANG Y S, et al. Changes of microstructure and mineralized tissue in the middle and late phase of osteoporotic fracture healing in rats[J]. Bone, 2007, 41(4):631-638.
5. WU C C, LI X B, HAN T S, et al. Dietary pseudopurpurin effects on bone mineral density and bone geometry architecture in rats[J]. Int J Mol Sci, 2012, 13(3): 3431-3443.
6. NORTON S A. Useful plants of dermatology.Ⅳ. Alizarin red and madder [J]. J Am Acad Dermatol, 1998, 39(3): 484-485.
7. INOUE K, YOSHIDA M, TAKAHASHI M, et al. Carcinogenic potential of alizarin and rubiadin, components of madder color, in a rat medium-term multi-organ bioassay[J]. Cancer Sci, 2009, 100(12): 2261-2267.
8. MAIER M S, GÓMEZ B A, PARERA S D, et al. Characterization of cultural remains associated to a human skeleton found at the site HMS Swift (1770)[J]. J Mol Struct, 2010, 978(1-3):191-194.
9. WU C C, WANG J G, LI P, et al. Bone mineral density and elemental composition in bone tissues in "red-boned" Guishan goats[J]. Biol Trace Elem Res, 2012, 149(3): 340-344.
10. WANG Y, BATTIÉ M C, BOYD S K, et al. The osseous endplates in lumbar vertebrae: Thickness, bone mineral density and their associations with age and disk degeneration[J]. Bone, 2011, 48(4): 804-809.
11. BERGOT C, WU Y, JOLIVET E, et al. The degree and distribution of cortical bone mineralization in the human femoral shaft change with age and sex in a microradiographic study[J]. Bone, 2009, 45(3): 435-442.
12. SEŠELJ M, NAHHAS R W, SHERWOOD R J, et al. The influence of age at menarche on cross-sectional geometry of bone in young adulthood[J]. Bone, 2012, 51(1): 38-45.
13. CAMPBELL G M, BERNHARDT R, SCHARNWEBER D, et al. The bone architecture is enhanced with combined PTH and alendronate treatment compared to monotherapy while maintaining the state of surface mineralization in the OVX rat[J]. Bone, 2011, 49(2):225-232.
14. NATALI A N, CARNIEL E L, PAVAN P G.. Dental implants press fit phenomena: Biomechanical analysis considering bone inelastic response[J]. Dent Mater, 2009, 25(5):573-581.
15. HÖGLER W, BLIMKIE C J R, COWELL C T, et al. A comparison of bone geometry and cortical density at the mid-femur between prepuberty and young adulthood using magnetic resonance imaging[J]. Bone, 2003, 33(5):771-778..

Journal of Biomedical Engineering

Effects of Madder on Bone Biomechanical Property in Rats

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