Experimental Research on the Prevention of Rabbit Postoperative Abdominal Cavity Adhesion with PLGA Membrane_Journal of Biomedical Engineering

Authors：

PANGXiubing ¹ , PANYongming ² , HUAFei ¹ , SUNChaoying ¹ , CHENLiang ² , CHENFangming ² , ZHUKeyan ² , XUJianqin ² ,  CHENMinli ²

1. Zhejiang Apeloa Medical Technology Co., Ltd., Dongyang 322118, China;
2. Laboratory Animal Research Center, Zhejiang Chinese Medical University, Hangzhou 310053, China;

Corresponding author：

CHENMinli, Email: minlichen01@aliyun.com

Keywords：

poly (lactic-co-glycotic acid) membrane; collagen fiber; transforming growth factor beta 1; postoperative abdominal cavity adhesion

DOI：

10.7507/1001-5515.20150027

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The aim of this paper is to explore the prevention of rabbit postoperative abdominal cavity adhesion with poly (lactic-co-glycotic acid) (PLGA) membrane and the mechanism of this prevention function. Sixty-six Japanese white rabbits were randomly divided into normal control group, model control group and PLGA membrane group. The rabbits were treated with multifactor methods to establish the postoperative abdominal cavity adhesion models except for those in the normal control group. PLGA membrane was used to cover the wounds of rabbits in the PLGA membrane group and nothing covered the wounds of rabbits in the model control group. The hematologic parameters, liver and kidney functions and fibrinogen contents were detected at different time. The rabbit were sacrificed 1, 2, 4, 6, 12 weeks after the operations, respectively. The adhesions were graded blindly, and Masson staining and immunohistochemistry methods were used to observe the proliferation of collagen fiber and the expression of transforming growth factorβ₁ (TGF-β₁) on the cecal tissues, respectively. The grade of abdominal cavity adhesion showed that the PLGA membrane-treated group was significant lower than that in the model control group, and it has no influence on liver and kidney function and hematologic parameters. But the fibrinogen content and the number of white blood cell in the PLGA membrane group were significant lower than those of model control group1 week and 2 weeks after operation, respectively. The density of collagen fiber and optical density of TGF-β₁ in the PLGA membrane group were significant lower than those of model control group. The results demonstrated that PLGA membrane could be effective in preventing the abdominal adhesions in rabbits, and it was mostly involved in the reducing of fibrinogen exudation, and inhibited the proliferation of collagen fiber and over-expression of TGF-β₁.

Citation： PANGXiubing, PANYongming, HUAFei, SUNChaoying, CHENLiang, CHENFangming, ZHUKeyan, XUJianqin, CHENMinli. Experimental Research on the Prevention of Rabbit Postoperative Abdominal Cavity Adhesion with PLGA Membrane. Journal of Biomedical Engineering, 2015, 32(1): 146-151, 162. doi: 10.7507/1001-5515.20150027 Copy

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2.	SHAHRAM E, SADRAIE S H, KAKA G, et al. Evaluation of chitosan-gelatin films for use as postoperative adhesion barrier in rat cecum model[J]. Int J Surg, 2013, 11(10): 1097-1102.
3.	JIANG D, XU C, LI Z, et al. Protective action of hepatocyte growth factor on transforming growth factor beta-1-induced alpha-smooth muscle actin and extracellular matrix in cultured human peritoneal fibroblasts[J]. Med Sci Monit, 2010, 16(8): BR250-BR254.
4.	曾煦欣, 赵洁, 黄嗣航, 等.维通注射剂对兔腹腔术后粘连防治作用的研究[J].中药材, 2008, 31(4): 580-584.
5.	PHILLIPS R K, DUDLEY H A. The effect of tetracycline lavage and trauma on visceral and parietal peritoneal ultrastructure and adhesion formation[J]. Br J Surg, 1984, 71(7): 537-539.
6.	MUNIREDDY S, KAVALUKAS S L, BARBUL A. Intra-abdominal healing: gastrointestinal tract and adhesions[J]. Surg Clin North Am, 2010, 90(6): 1227-1236.
7.	ARUNG W, MEURISSE M, DETRY O. Pathophysiology and prevention of postoperative peritoneal adhesions[J]. World J Gastroenterol, 2011, 17(41): 4545-4553.
8.	SCHNVRIGER B, BARMPARAS G, BRANCO B C, et al. Prevention of postoperative peritoneal adhesions: a review of the literature[J]. Am J Surg, 2011, 201(1): 111-121.
9.	刘秀花.透明质酸在妇产科腹盆腔手术术后粘连预防中的临床效果观察[J].中国社区医师, 2013, 15(8): 117-118.
10.	李彦明, 李冬青, 陈鹏, 等.聚乙丙交酯膜预防硬膜外瘢痕粘连的实验研究[J].山东大学学报:医学版, 2009, 47(6):33-38.
11.	庞迪, 叶京兵, 陈栋梁, 等.PLLA和PLGA体内早期降解的对比研究[J].生物医学工程学杂志, 2010, 27(6): 1298-1302.
12.	赵健, 刘晓程, 孔祥荣, 等.聚乳酸/乙醇酸复合bFGF降解支架在心肌血运重建中的应用实验[J].心脏杂志, 2009, 21(3): 383-386.
13.	DINARVAND P, HASHEMI S M, SEYEDJAFARI E, et al. Function of poly (lactic-co-glycolic acid) nanofiber in reduction of adhesion bands[J]. J Surg Res, 2012, 172(1): e1-e9.
14.	MARTINS E QUININO R, ARAUJO-FILHO I, LIMA F P, et al. Adhesion prevention in reabsorbable polyethylene glycol hydrogel (Coseal?) coated polypropylene mesh in rabbits[J]. Acta Cir Bras, 2013, 28(12): 807-814.
15.	FALK P, ANGENETE E, BERGSTROM M, et al. TGF-β₁ promotes transition of mesothelial cells into fibroblast phenotype in response to peritoneal injury in a cell culture model[J]. Int J Surg, 2013, 11(9): 977-982.
16.	FARHAT Y M, AL-MALIKI A A, CHEN T, et al. Gene expression analysis of the pleiotropic effects of TGF-β₁ in an in vitro model of flexor tendon healing[J]. PLoS One, 2012, 7(12): e51411.

1. FOMETESCU S G, COSTACHE M, COVENEY A, et al. Peritoneal fibrinolytic activity and adhesiogenesis[J]. Chirurgia (Bucur), 2013, 108(3): 331-340.
2. SHAHRAM E, SADRAIE S H, KAKA G, et al. Evaluation of chitosan-gelatin films for use as postoperative adhesion barrier in rat cecum model[J]. Int J Surg, 2013, 11(10): 1097-1102.
3. JIANG D, XU C, LI Z, et al. Protective action of hepatocyte growth factor on transforming growth factor beta-1-induced alpha-smooth muscle actin and extracellular matrix in cultured human peritoneal fibroblasts[J]. Med Sci Monit, 2010, 16(8): BR250-BR254.
4. 曾煦欣, 赵洁, 黄嗣航, 等.维通注射剂对兔腹腔术后粘连防治作用的研究[J].中药材, 2008, 31(4): 580-584.
5. PHILLIPS R K, DUDLEY H A. The effect of tetracycline lavage and trauma on visceral and parietal peritoneal ultrastructure and adhesion formation[J]. Br J Surg, 1984, 71(7): 537-539.
6. MUNIREDDY S, KAVALUKAS S L, BARBUL A. Intra-abdominal healing: gastrointestinal tract and adhesions[J]. Surg Clin North Am, 2010, 90(6): 1227-1236.
7. ARUNG W, MEURISSE M, DETRY O. Pathophysiology and prevention of postoperative peritoneal adhesions[J]. World J Gastroenterol, 2011, 17(41): 4545-4553.
8. SCHNVRIGER B, BARMPARAS G, BRANCO B C, et al. Prevention of postoperative peritoneal adhesions: a review of the literature[J]. Am J Surg, 2011, 201(1): 111-121.
9. 刘秀花.透明质酸在妇产科腹盆腔手术术后粘连预防中的临床效果观察[J].中国社区医师, 2013, 15(8): 117-118.
10. 李彦明, 李冬青, 陈鹏, 等.聚乙丙交酯膜预防硬膜外瘢痕粘连的实验研究[J].山东大学学报:医学版, 2009, 47(6):33-38.
11. 庞迪, 叶京兵, 陈栋梁, 等.PLLA和PLGA体内早期降解的对比研究[J].生物医学工程学杂志, 2010, 27(6): 1298-1302.
12. 赵健, 刘晓程, 孔祥荣, 等.聚乳酸/乙醇酸复合bFGF降解支架在心肌血运重建中的应用实验[J].心脏杂志, 2009, 21(3): 383-386.
13. DINARVAND P, HASHEMI S M, SEYEDJAFARI E, et al. Function of poly (lactic-co-glycolic acid) nanofiber in reduction of adhesion bands[J]. J Surg Res, 2012, 172(1): e1-e9.
14. MARTINS E QUININO R, ARAUJO-FILHO I, LIMA F P, et al. Adhesion prevention in reabsorbable polyethylene glycol hydrogel (Coseal?) coated polypropylene mesh in rabbits[J]. Acta Cir Bras, 2013, 28(12): 807-814.
15. FALK P, ANGENETE E, BERGSTROM M, et al. TGF-β₁ promotes transition of mesothelial cells into fibroblast phenotype in response to peritoneal injury in a cell culture model[J]. Int J Surg, 2013, 11(9): 977-982.
16. FARHAT Y M, AL-MALIKI A A, CHEN T, et al. Gene expression analysis of the pleiotropic effects of TGF-β₁ in an in vitro model of flexor tendon healing[J]. PLoS One, 2012, 7(12): e51411.

Journal of Biomedical Engineering

Experimental Research on the Prevention of Rabbit Postoperative Abdominal Cavity Adhesion with PLGA Membrane

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