Research on Molecular Biological Characteristics of Proto-oncogene pim-2_Journal of Biomedical Engineering

Authors：

ZHOUXiaodong ,  SHENFubing

School of Laboratory Medicine, Chengdu Medical College, Chengdu 610500, China;

Corresponding author：

SHENFubing, Email: shenfubing@163.com

Keywords：

proto-oncogene pim-2; bioinformatics; protein modification

DOI：

10.7507/1001-5515.20150073

Video：

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The purpose of this paper is to present the research on the molecular biological characteristics of proto-oncogene pim-2 and to analyze the related mechanism. Proto-oncogene pim-2 was studied and analyzed by the bioinformatics method and technology. With an online server, the chromosomal localization of pim-2 gene was analyzed, and the exon, open reading frame, CpG island and miRNAs complementary fragments and the like were predicted. With bioinformatics software, the physicochemical property of transcription protein of proto-oncogene pim-2 and various modification sites of protein sequence, such as ubiquitination and glycosylation, were predicted, the antigenic index was calculated, and the spatial structural was modeled. The research findings showed that the proto-oncogene pim-2 comprised six exons, the CDS (coding sequence) transcribed a section of peptide chain including 311 amino acids, a gene promoter has a CpG island, and the 3'UTR region contains an miRNA gene. The molecular weight of the Pim-2 protein was 34, 188.47, the isoelectric point was 5.78, the instability index was 45.87, and the extinction coefficient was 279nm. A plurality of covalent modification sites, two ubiquitination sites, four glycosylation sites, an SUMO sumoylation site, a nitrosation site, two palmitoylation sites and sixteen regions with higher antigenic index were distributed in the protein sequence. This research showed that the related regions and modification sites distributed on the sequence of proto-oncogene pim-2 were closely related to the carcinogenic effect thereof.

Citation： ZHOUXiaodong, SHENFubing. Research on Molecular Biological Characteristics of Proto-oncogene pim-2. Journal of Biomedical Engineering, 2015, 32(2): 405-411. doi: 10.7507/1001-5515.20150073 Copy

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1. LEWIS B P, BURGE C B, BARTEL D P. Conserved seed pairing, often flanked by adenosines, indicates that thousands of human genes are microRNA targets[J]. Cell, 2005, 120(1):15-20.
2. 覃玉桃, 王仁生.微小RNA与鼻咽癌[J].医学综述, 2011, 17(7):993-996.
3. 陈红.HER2与乳腺癌临床-病理关系的前瞻性分析及其miRNAs调控研究[D].重庆:第三军医大学, 2009.
4. HUANG Tao, WANG Ping, YE Zhiqiang, et al. Prediction of deleterious non-synonymous SNPs based on protein interaction network and hybrid properties[J]. PLoS One, 2010, 5(7):e11900.
5. HULO N, BAIROCH A, BULLIARD V, et al. The 20 years of PROSITE[J]. Nucleic Acids Res, 2008, 36 (Database issue):D245-D249.
6. SELDIN D C, LANDESMAN-BOLLAG E, FARAGO M, et al. CK2 as a positive regulator of Wnt signalling and tumourigenesis[J]. Mol Cell Biochem, 2005, 274(1-2):63-67.
7. PEREA S E, REYES O, BALADRON I, et al. CIGB-300, a novel proapoptotic peptide that impairs the CK2 phosphorylation and exhibits anticancer properties both in vitro and in vivo[J]. Mol Cell Biochem, 2008, 316(1-2):163-167.
8. 魏小华, 梁杰.蛋白激酶CK2在肿瘤生物学中的作用[J].医学综述, 2011, 17(1):75-77.
9. DANIELSEN J M R, SYLVESTERSEN K B, BEKKER-JENSEN S, et al. Mass spectrometric analysis of lysine ubiquitylation reveals promiscuity at site level[J]. Mol Cell Proteomics, 2011, 10(3):M110.003590.
10. 倪晓光, 赵平.泛素-蛋白酶体途径与恶性肿瘤关系的研究进展[J].中华肿瘤防治杂志, 2007, 14(19):1512-1515.
11. 严钦, 俞慧清, 成国祥.蛋白糖基化与免疫研究进展[J].现代免疫学, 2008, 28(2):165-168.
12. HART G W, COPELAND R J. Glycomics hits the big time[J]. Cell, 2010, 143(5):672-676.
13. LIU Boshu, LI Sujun, WANG Yinglin, et al. Predicting the protein SUMO modification sites based on Properties Sequential Forward Selection (PSFS)[J]. Biochem Biophys Res Commun, 2007, 358(1):136-139.
14. 胡丹, 陈福祥.SUMO化与去SUMO化在肿瘤发生中的作用[J].国际肿瘤学杂志, 2012, 39(6):406-408.
15. MIAO Feng, NATARAJAN R. Mapping global histone methylation patterns in the coding regions of human genes[J]. Mol Cell Biol, 2005, 25(11):4650-4661.
16. 张晓静, 丛斌.蛋白质巯基亚硝基化对细胞功能的调控作用[J].中国病理生理杂志, 2011, 27(11):2237-2240.
17. 褚秀玲, 苏建青, 靳书刚, 等.马立克氏病毒pp38基因的克隆测序和B细胞抗原表位预测[J].中国兽医学报, 2007, 27(4):451-454.
18. 秦静, 于士颜, 李锦军.蛋白质棕榈酰化修饰及其研究方法[J].生命的化学, 2008, 28(5):595-598.

Journal of Biomedical Engineering

Research on Molecular Biological Characteristics of Proto-oncogene pim-2

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