Research Progress in Preparation of Antimicrobial Peptides and Their Mechanisms of Action_Journal of Biomedical Engineering

Authors：

LIYan , WANGJiarong , SUNHongbin

High Magnetic Field Laboratory, Heifei Institutes of Physical Science, Chinese Academy of Science, Hefei 230031, China;

Corresponding author：

Keywords：

antimicrobial peptides; antibacterial mechanisms; cell membranes

DOI：

10.7507/1001-5515.20150084

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Currently, all the conventional antibiotics have developed corresponding drug-resistant pathogenic strains, which have increasingly become a serious threat to people's health. Development of completely new types of antibiotics is one of effective ways to solve the drug resistance issue. Antimicrobial peptides with broad-spectrum antibacterial and antimicrobial activity and wild variety become the ideal alternative to traditional antibiotics. Antimicrobial peptides are derived from wide range of sources, such as plants, animals, and microorganisms. Mechanism of function of the antimicrobial peptides and the investigation approaches of different antimicrobial peptides also vary dramatically. In this paper, we give an overview of preparation, antibacterial mechanisms, and research methodology of antimicrobial peptides.

Citation： LIYan, WANGJiarong, SUNHongbin. Research Progress in Preparation of Antimicrobial Peptides and Their Mechanisms of Action. Journal of Biomedical Engineering, 2015, 32(2): 465-469. doi: 10.7507/1001-5515.20150084 Copy

1.	SILVA N C, SARMENTO B, PINTADO M.. The importance of antimicrobial peptides and their potential for therapeutic use in ophthalmology[J]. Int J Antimicrob Agents, 2013, 41(1):5-10.
2.	NIU Y, WU H, LI Y, et al. AApeptides as a new class of antimicrobial agents[J]. Org Biomol Chem, 2013, 11(26):4283-4290.
3.	ISAKSSON J, BRANDSDAL B O, ENGQVIST M, et al. A synthetic antimicrobial peptidomimetic (LTX 109):stereochemical impact on membrane disruption[J]. Med Chem, 2011, 54(16):5786-5795.
4.	单安山, 马得莹, 冯兴军, 等.抗菌肽的功能、研发与应用[J].中国农业科学, 2012, 45(11):2249-2259.
5.	SPERSTAD S V, HAUG T, BLENCKE H M, et al. Antimicrobial peptides from Marine invertebrates:challenges and perspectives in Marine antimicrobial peptide discovery[J]. Biotechnol Adv, 2011, 29(5):519-530.
6.	WIRADHARMA N, KHOE U, HAUSER CA, et al. Synthetic cationic amphiphilicαhelical peptides as antimicrobial agents[J]. Biomaterials, 2011, 32(8):2204-2212.
7.	XIE Y G, LUAN C, ZHANG H W, et al. Effects of thioredoxin:SUMO and intein on soluble fusion expression of an antimicrobial peptide OG2 in Escherichia coli[J]. Protein Pept Lett, 2013, 20(1):54-60.
8.	YANG K, SU Y, LI J, et al. Expression and purification of the antimicrobial peptide cecropin AD by fusion with cationic elastin-like polypeptides[J]. Protein Expr Purif, 2012, 85(2):200-203.
9.	袁永俊, 胡婷, 朱家骅.酪蛋白抗菌肽的酶法制备[J].食品与机械, 2010, 26(2):1-4.
10.	TEIXEIRA V, FEIO M J, BASTOS M. Role of lipids in the interaction of antimicrobial peptides with membranes[J]. Prog Lipid Res, 2012, 51(2):149-177.
11.	VARKOUHI A K, SCHOLTE M, STORM G, et al. Endosomal escape pathways for delivery of biologicals[J]. J Control Release, 2011, 151(3):220-228.
12.	SALOMONE F, CARDARELLI F, DI LUCA M, et al. A novel chimeric cell-penetrating peptide with membrane-disruptive properties for efficient endosomal escape[J]. J Control Release, 2012, 163(3):293-303.
13.	SALOMONE F, CARDARELLI F, SIGNORE G, et al. In vitro efficient transfection by CM18-Tat11 hybrid peptide:a new tool for gene-delivery applications[J]. PLoS One, 2013, 8(7):e70108.
14.	WIMLEY W C, HRISTOVA K. Antimicrobial peptides:successes, challenges and unanswered questions[J]. J Membr Biol, 2011, 239(1-2):27-34.
15.	TRAVKOVA O G, ANDRA J, MOHWALD H, et al. Influence of arenicin on phase transitions and ordering of lipids in 2D model membranes[J]. American Chemical Society, 2013, 29(39):12203-12211.
16.	MARR A K, MCGWIRE B S, MCMASTER W R. Modes of action of Leishmanicidal antimicrobial peptides[J]. Future Microbiol, 2012, 7(9):1047-1059.
17.	CHO J, HWANG I S, CHOI H, et al. The novel biological action of antimicrobial peptides via apoptosis induction[J]. J Microbiol Biotechnol, 2012, 22(11):1457-1466.
18.	CRUCIANI R A, BARKER J L, ZASLOFF M, et al. Antibiotic magainins exert cytolytic activity against transformed cell lines through Channel formation[J]. Proc Natl Acad Sci, 1991, 88(9):3792-3796.
19.	TORRENT M, ANDREU D, NOGUÉS V M, et al. Connecting peptide physicochemical and antimicrobial properties by a rational prediction model[J]. PLoS One, 2011, 6(2):e16968.
20.	BOBEK L A, SITU H. MUC7 20-Mer:investigation of antimicrobial activity, secondary structure, and possible mechanism of antifungal action[J]. Peptides, 2003, 47(2):643-652.
21.	HWANG B, HWANG J S, LEE J, et al. The antimicrobial peptide, psacotheasin induces reactive Oxygen species and triggers apoptosis in Candida albicans[J]. Biochem Biophys Res Commun, 2011, 405(2):267-271.
22.	尚田田, 徐彦召, 杭柏林, 等.抗菌肽的抗菌机理与构效关系研究进展[J].黑龙江畜牧兽医, 2013, 5(上):26-28.
23.	URSIC-BEDOYA R, BUCHHOP J, JOY J B, et al. Prolixicin:a novel antimicrobial peptide isolated from Rhodnius prolixus with differential activity against bacteria and Trypanosoma cruzi[J]. Insect Mol Biol, 2011, 20(6):775-786.
24.	LOGIN F H, BALMAND S, VALLIER A, et al. Antimicrobial peptides keep insect endosymbionts under control[J]. Science, 2011, 334(6054):362-365.
25.	EPAND R F, EPAND R M, MONACO V, et al. The antimicrobial peptide trichogin and its interaction with phospholipid membranes[J]. Eur J Biochem, 1999, 266(3):1021-1028.
26.	VOGEL H J, SCHIBLI D J, JING W, et al. Towards a structure-function analysis of bovine lactoferricin and related tryptophan-and arginine-containing peptides[J]. Biochem Cell Biol, 2002, 80(1):49-63.
27.	HANEY E F, HUNTER H N, MATSUZAKI K, et al. Solution NMR studies of amphibian antimicrobial peptides:linking structure to function?[J]. Biochim Biophys Acta, 2009, 1788(8):1639-1655.
28.	FERNANDEZ D I, SANI M A, GEHMAN J D, et al. Interactions of a synthetic Leu-Lys-rich antimicrobial peptide with phospholipid bilayers[J]. Eur Biophys, 2011, 40(4):471-480.
29.	STAUDEGGER E, PRENNER E J, KRIECHBAUM M, et al. X-ray studies on the interaction of the antimicrobial peptide gramicidin S with microbial lipid extracts:evidence for cubic phase formation[J]. Biochim Biophys Acta, 2000, 1468(1-2):213-230.
30.	CHOI H, HWANG J S, LEE D G. Antifungal effect and pore-forming action of lactoferricin B like peptide derived from centipede Scolopendra subspinipes mutilans[J]. Biochim Biophys Acta, 2013, 1828(11):2745-2750.
31.	LI L, SHI Y, CHESEREK M J, et al. Antibacterial activity and dual mechanisms of peptide analog derived from cell-penetrating peptide against Salmonella typhimurium and Streptococcus pyogenes[J]. Appl Microbiol Biotechnol, 2013, 97(4):1711-1723.
32.	EVAN F H, KAMRAN N, JAN G M, et al. Structural and biophysical characterization of an antimicrobial peptide chimera comprised of lactoferricin and lactoferrampin[J]. Biochim Biophys Acta, 2011, 1818(3):762-775.
33.	郝刚, 乐国伟, 施用晖.抗菌肽BuforinⅡ衍生物对金黄色葡萄球菌细胞膜作用机制的研究[J].畜牧兽医学报, 2013, 44(7):1124-1130.

1. SILVA N C, SARMENTO B, PINTADO M.. The importance of antimicrobial peptides and their potential for therapeutic use in ophthalmology[J]. Int J Antimicrob Agents, 2013, 41(1):5-10.
2. NIU Y, WU H, LI Y, et al. AApeptides as a new class of antimicrobial agents[J]. Org Biomol Chem, 2013, 11(26):4283-4290.
3. ISAKSSON J, BRANDSDAL B O, ENGQVIST M, et al. A synthetic antimicrobial peptidomimetic (LTX 109):stereochemical impact on membrane disruption[J]. Med Chem, 2011, 54(16):5786-5795.
4. 单安山, 马得莹, 冯兴军, 等.抗菌肽的功能、研发与应用[J].中国农业科学, 2012, 45(11):2249-2259.
5. SPERSTAD S V, HAUG T, BLENCKE H M, et al. Antimicrobial peptides from Marine invertebrates:challenges and perspectives in Marine antimicrobial peptide discovery[J]. Biotechnol Adv, 2011, 29(5):519-530.
6. WIRADHARMA N, KHOE U, HAUSER CA, et al. Synthetic cationic amphiphilicαhelical peptides as antimicrobial agents[J]. Biomaterials, 2011, 32(8):2204-2212.
7. XIE Y G, LUAN C, ZHANG H W, et al. Effects of thioredoxin:SUMO and intein on soluble fusion expression of an antimicrobial peptide OG2 in Escherichia coli[J]. Protein Pept Lett, 2013, 20(1):54-60.
8. YANG K, SU Y, LI J, et al. Expression and purification of the antimicrobial peptide cecropin AD by fusion with cationic elastin-like polypeptides[J]. Protein Expr Purif, 2012, 85(2):200-203.
9. 袁永俊, 胡婷, 朱家骅.酪蛋白抗菌肽的酶法制备[J].食品与机械, 2010, 26(2):1-4.
10. TEIXEIRA V, FEIO M J, BASTOS M. Role of lipids in the interaction of antimicrobial peptides with membranes[J]. Prog Lipid Res, 2012, 51(2):149-177.
11. VARKOUHI A K, SCHOLTE M, STORM G, et al. Endosomal escape pathways for delivery of biologicals[J]. J Control Release, 2011, 151(3):220-228.
12. SALOMONE F, CARDARELLI F, DI LUCA M, et al. A novel chimeric cell-penetrating peptide with membrane-disruptive properties for efficient endosomal escape[J]. J Control Release, 2012, 163(3):293-303.
13. SALOMONE F, CARDARELLI F, SIGNORE G, et al. In vitro efficient transfection by CM18-Tat11 hybrid peptide:a new tool for gene-delivery applications[J]. PLoS One, 2013, 8(7):e70108.
14. WIMLEY W C, HRISTOVA K. Antimicrobial peptides:successes, challenges and unanswered questions[J]. J Membr Biol, 2011, 239(1-2):27-34.
15. TRAVKOVA O G, ANDRA J, MOHWALD H, et al. Influence of arenicin on phase transitions and ordering of lipids in 2D model membranes[J]. American Chemical Society, 2013, 29(39):12203-12211.
16. MARR A K, MCGWIRE B S, MCMASTER W R. Modes of action of Leishmanicidal antimicrobial peptides[J]. Future Microbiol, 2012, 7(9):1047-1059.
17. CHO J, HWANG I S, CHOI H, et al. The novel biological action of antimicrobial peptides via apoptosis induction[J]. J Microbiol Biotechnol, 2012, 22(11):1457-1466.
18. CRUCIANI R A, BARKER J L, ZASLOFF M, et al. Antibiotic magainins exert cytolytic activity against transformed cell lines through Channel formation[J]. Proc Natl Acad Sci, 1991, 88(9):3792-3796.
19. TORRENT M, ANDREU D, NOGUÉS V M, et al. Connecting peptide physicochemical and antimicrobial properties by a rational prediction model[J]. PLoS One, 2011, 6(2):e16968.
20. BOBEK L A, SITU H. MUC7 20-Mer:investigation of antimicrobial activity, secondary structure, and possible mechanism of antifungal action[J]. Peptides, 2003, 47(2):643-652.
21. HWANG B, HWANG J S, LEE J, et al. The antimicrobial peptide, psacotheasin induces reactive Oxygen species and triggers apoptosis in Candida albicans[J]. Biochem Biophys Res Commun, 2011, 405(2):267-271.
22. 尚田田, 徐彦召, 杭柏林, 等.抗菌肽的抗菌机理与构效关系研究进展[J].黑龙江畜牧兽医, 2013, 5(上):26-28.
23. URSIC-BEDOYA R, BUCHHOP J, JOY J B, et al. Prolixicin:a novel antimicrobial peptide isolated from Rhodnius prolixus with differential activity against bacteria and Trypanosoma cruzi[J]. Insect Mol Biol, 2011, 20(6):775-786.
24. LOGIN F H, BALMAND S, VALLIER A, et al. Antimicrobial peptides keep insect endosymbionts under control[J]. Science, 2011, 334(6054):362-365.
25. EPAND R F, EPAND R M, MONACO V, et al. The antimicrobial peptide trichogin and its interaction with phospholipid membranes[J]. Eur J Biochem, 1999, 266(3):1021-1028.
26. VOGEL H J, SCHIBLI D J, JING W, et al. Towards a structure-function analysis of bovine lactoferricin and related tryptophan-and arginine-containing peptides[J]. Biochem Cell Biol, 2002, 80(1):49-63.
27. HANEY E F, HUNTER H N, MATSUZAKI K, et al. Solution NMR studies of amphibian antimicrobial peptides:linking structure to function?[J]. Biochim Biophys Acta, 2009, 1788(8):1639-1655.
28. FERNANDEZ D I, SANI M A, GEHMAN J D, et al. Interactions of a synthetic Leu-Lys-rich antimicrobial peptide with phospholipid bilayers[J]. Eur Biophys, 2011, 40(4):471-480.
29. STAUDEGGER E, PRENNER E J, KRIECHBAUM M, et al. X-ray studies on the interaction of the antimicrobial peptide gramicidin S with microbial lipid extracts:evidence for cubic phase formation[J]. Biochim Biophys Acta, 2000, 1468(1-2):213-230.
30. CHOI H, HWANG J S, LEE D G. Antifungal effect and pore-forming action of lactoferricin B like peptide derived from centipede Scolopendra subspinipes mutilans[J]. Biochim Biophys Acta, 2013, 1828(11):2745-2750.
31. LI L, SHI Y, CHESEREK M J, et al. Antibacterial activity and dual mechanisms of peptide analog derived from cell-penetrating peptide against Salmonella typhimurium and Streptococcus pyogenes[J]. Appl Microbiol Biotechnol, 2013, 97(4):1711-1723.
32. EVAN F H, KAMRAN N, JAN G M, et al. Structural and biophysical characterization of an antimicrobial peptide chimera comprised of lactoferricin and lactoferrampin[J]. Biochim Biophys Acta, 2011, 1818(3):762-775.
33. 郝刚, 乐国伟, 施用晖.抗菌肽BuforinⅡ衍生物对金黄色葡萄球菌细胞膜作用机制的研究[J].畜牧兽医学报, 2013, 44(7):1124-1130.

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