Research Progress of Application of Microfluidics Techniques in Cryopreservation_Journal of Biomedical Engineering

Authors：

ZHOUNanfeng , YangYun ,  ZHOUXinli

Institute of Biothermal Technology, University of Shanghai for Science and Technology, Shanghai 200093, China;

Corresponding author：

ZHOUXinli, Email: zjulily@163.com

Keywords：

cryopreservation; microfluidics; cryoprotectant

DOI：

10.7507/1001-5515.20150128

Video：

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Microfluidics technology may be an effective method to solve some problems in cryopreservation. This review presents the research progress of microfluidics technology in the field of cell membrane transport properties, cryoprotectant addition and washout and the vitrification for cryopreservation of biological materials. Existing problems of microfluidics technology in the application of cryopreservation are summarized and future research directions are indicated as well.

Citation： ZHOUNanfeng, YangYun, ZHOUXinli. Research Progress of Application of Microfluidics Techniques in Cryopreservation. Journal of Biomedical Engineering, 2015, 32(3): 702-706. doi: 10.7507/1001-5515.20150128 Copy

1.	ATABAY E P, ATABAY E C, CRUZ L C. Cryotop and solid surface vitrification cryodevices are suitable for the cryopreservation of in vitro-matured water buffalo(bubalus bubalis L.)oocytes[J]. Philippine Journal of Veterinary Medicine, 2013, 50(1):24-33.
2.	ALMANSOORI K A, PRASAD V, FORBES J F, et al. Cryoprotective agent toxicity interactions in human articular chondrocytes[J]. Cryobiology, 2012, 64(3):185-191.
3.	INOUE M, OKONOGI A, TERAO K, et al. Cell culture on MEMS materials in microenvironment limited by a physical condition[J]. Micro Nano Lett, 2012, 7(8):725-728.
4.	HUANG S B, WU M H, WANG S S, et al. Microfluidic cell culture chip with multiplexed medium delivery and efficient cell/scaffold loading mechanisms for high-throughput perfusion 3-dimensional cell culture-based assays[J]. Biomed Microdevices, 2011, 13(3):415-430.
5.	INAMDAR N K, BORENSTEIN J T. Microfluidic cell culture models for tissue engineering[J]. Curr Opin Biotechnol, 2011, 22(5):681-689.
6.	REN Y, CHOW L M, LEUNG W W. Cell culture using centrifugal microfluidic platform with demonstration on Pichia pastoris[J]. Biomed Microdevices, 2013, 15(2):321-337.
7.	CAO Z, CHEN F, BAO N, et al. Droplet sorting based on the number of encapsulated particles using a solenoid valve[J]. Lab Chip, 2013, 13(1):171-178.
8.	QIN W, SCHMIDT L, YANG X, et al. Laser guidance-based cell detection in a microfluidic biochip[J]. J Biomed Opt, 2013, 18(6):060502.
9.	MU X, ZHENG W, SUN J, et al. Microfluidics for manipulating cells[J]. Small, 2013, 9(1):9-21.
10.	VICKERS D L, HINCAPIE M, HANCOCK W S, et al. Lectin-mediated microfluidic capture and release of leukemic lymphocytes from whole blood[J]. Biomed Microdevices, 2011, 13(3):565-571.
11.	MARTINEZ C J, KIM J W, YE C, et al. A microfluidic approach to encapsulate living cells in uniform alginate hydrogel microparticles[J]. Macromol Biosci, 2012, 12(7):946-951.
12.	FAN Y, XU F, HUANG G, et al. Single neuron capture and axonal development in three-dimensional microscale hydrogels[J]. Lab Chip, 2012, 12(22):4724-4731.
13.	METTO E C, EVANS K, BARNEY P, et al. An integrated microfluidic device for monitoring changes in nitric oxide production in single T-lymphocyte (Jurkat) cells[J]. Anal Chem, 2013, 85(21):10188-10195.
14.	LAM B, FANG Z, SARGENT E H, et al. Polymerase chain reaction-free, sample-to-answer bacterial detection in 30 minutes with integrated cell lysis[J]. Anal Chem, 2012, 84(1):21-25.
15.	JHA S K, CHAND R, HAN D, et al. An integrated PCR microfluidic chip incorporating aseptic electrochemical cell lysis and capillary electrophoresis amperometric DNA detection for rapid and quantitative genetic analysis[J]. Lab Chip, 2012, 12(21):4455-4464.
16.	MERNIER G, MARTINEZ-DUARTE R, LEHAL R, et al. Very high throughput electrical cell lysis and extraction of intracellular compounds using 3D Carbon electrodes in Lab-on-a-chip devices[J]. Micromachines, 2012, 3(3):574-581.
17.	JEN C P, AMSTISLAVSKAYA T G, LIU Y H, et al. Single-cell electric lysis on an electroosmotic-driven microfluidic chip with arrays of microwells[J]. Sensors (Basel), 2012, 12(6):6967-6977.
18.	KIM J, HONG J W, KIM D P, et al. Nanowire-integrated microfluidic devices for facile and reagent-free mechanical cell lysis[J]. Lab Chip, 2012, 12(16):2914-2921.
19.	CHEN H H, SHEN H, HEIMFELD S, et al. A microfluidic study of mouse dendritic cell membrane transport properties of water and cryoprotectants[J]. Int J Heat Mass Transf, 2008, 51(23/24):5687-5694.
20.	WANG J, ZHU K, ZHAO G, et al. Dual dependence of cryobiogical properties of Sf21 cell membrane on the temperature and the concentration of the cryoprotectant[J]. PLoS One, 2013, 8(9):e72836.
21.	HUBEL A. Advancing the preservation of cellular therapy products[J]. Transfusion, 2011, 51(Suppl 4):82S-86S.
22.	FLEMING K K, LONGMIRE E K, HUBEL A. Numerical characterization of diffusion-based extraction in cell-laden flow through a microfluidic Channel[J]. J Biomech Eng, 2007, 129(5):703-711.
23.	MATA C, LONGMIRE E K, MCKENNA D H, et al. Experimental study of diffusion-based extraction from a cell suspension[J]. Microfluid Nanofluidics, 2008, 5(4):529-540.
24.	MATA C, LONGMIRE E, MCKENNA D, et al. Cell motion and recovery in a two-stream microfluidic device[J]. Microfluid Nanofluidics, 2010, 8(4):457-465.
25.	HANNA J, HUBEL A, LEMKE E. Diffusion-based extraction of DMSO from a cell suspension in a three stream, vertical microchannel[J]. Biotechnol Bioeng, 2012, 109(9):2316-2324.
26.	SONG Y S, MOON S, HULLI L, et al. Microfluidics for cryopreservation[J]. Lab Chip, 2009, 9(13):1874-1881.
27.	HEO Y S, LEE H J, HASSELL B A, et al. Controlled loading of cryoprotectants (CPAs) to oocyte with linear and complex CPA profiles on a microfluidic platform[J]. Lab Chip, 2011, 11(20):3530-3537.
28.	LI S, LIU W, LIN L. On-Chip cryopreservation of living cells[J]. Journal of the Association for Laboratory Automation, 2010, 15(2):99-106.
29.	ZOU Y, YIN T, CHEN S, et al. On-chip cryopreservation:a novel method for ultra-rapid cryoprotectant-free cryopreservation of small amounts of human spermatozoa[J]. PLoS One, 2013, 8(4):e61593.
30.	ZHOU X M, LIU Z, LIANG X M, et al. Theoretical investigations of a novel microfluidic cooling/warming system for cell vitrification cryopreservation[J]. Int J Heat Mass Transf, 2013, 65:381-388.

1. ATABAY E P, ATABAY E C, CRUZ L C. Cryotop and solid surface vitrification cryodevices are suitable for the cryopreservation of in vitro-matured water buffalo(bubalus bubalis L.)oocytes[J]. Philippine Journal of Veterinary Medicine, 2013, 50(1):24-33.
2. ALMANSOORI K A, PRASAD V, FORBES J F, et al. Cryoprotective agent toxicity interactions in human articular chondrocytes[J]. Cryobiology, 2012, 64(3):185-191.
3. INOUE M, OKONOGI A, TERAO K, et al. Cell culture on MEMS materials in microenvironment limited by a physical condition[J]. Micro Nano Lett, 2012, 7(8):725-728.
4. HUANG S B, WU M H, WANG S S, et al. Microfluidic cell culture chip with multiplexed medium delivery and efficient cell/scaffold loading mechanisms for high-throughput perfusion 3-dimensional cell culture-based assays[J]. Biomed Microdevices, 2011, 13(3):415-430.
5. INAMDAR N K, BORENSTEIN J T. Microfluidic cell culture models for tissue engineering[J]. Curr Opin Biotechnol, 2011, 22(5):681-689.
6. REN Y, CHOW L M, LEUNG W W. Cell culture using centrifugal microfluidic platform with demonstration on Pichia pastoris[J]. Biomed Microdevices, 2013, 15(2):321-337.
7. CAO Z, CHEN F, BAO N, et al. Droplet sorting based on the number of encapsulated particles using a solenoid valve[J]. Lab Chip, 2013, 13(1):171-178.
8. QIN W, SCHMIDT L, YANG X, et al. Laser guidance-based cell detection in a microfluidic biochip[J]. J Biomed Opt, 2013, 18(6):060502.
9. MU X, ZHENG W, SUN J, et al. Microfluidics for manipulating cells[J]. Small, 2013, 9(1):9-21.
10. VICKERS D L, HINCAPIE M, HANCOCK W S, et al. Lectin-mediated microfluidic capture and release of leukemic lymphocytes from whole blood[J]. Biomed Microdevices, 2011, 13(3):565-571.
11. MARTINEZ C J, KIM J W, YE C, et al. A microfluidic approach to encapsulate living cells in uniform alginate hydrogel microparticles[J]. Macromol Biosci, 2012, 12(7):946-951.
12. FAN Y, XU F, HUANG G, et al. Single neuron capture and axonal development in three-dimensional microscale hydrogels[J]. Lab Chip, 2012, 12(22):4724-4731.
13. METTO E C, EVANS K, BARNEY P, et al. An integrated microfluidic device for monitoring changes in nitric oxide production in single T-lymphocyte (Jurkat) cells[J]. Anal Chem, 2013, 85(21):10188-10195.
14. LAM B, FANG Z, SARGENT E H, et al. Polymerase chain reaction-free, sample-to-answer bacterial detection in 30 minutes with integrated cell lysis[J]. Anal Chem, 2012, 84(1):21-25.
15. JHA S K, CHAND R, HAN D, et al. An integrated PCR microfluidic chip incorporating aseptic electrochemical cell lysis and capillary electrophoresis amperometric DNA detection for rapid and quantitative genetic analysis[J]. Lab Chip, 2012, 12(21):4455-4464.
16. MERNIER G, MARTINEZ-DUARTE R, LEHAL R, et al. Very high throughput electrical cell lysis and extraction of intracellular compounds using 3D Carbon electrodes in Lab-on-a-chip devices[J]. Micromachines, 2012, 3(3):574-581.
17. JEN C P, AMSTISLAVSKAYA T G, LIU Y H, et al. Single-cell electric lysis on an electroosmotic-driven microfluidic chip with arrays of microwells[J]. Sensors (Basel), 2012, 12(6):6967-6977.
18. KIM J, HONG J W, KIM D P, et al. Nanowire-integrated microfluidic devices for facile and reagent-free mechanical cell lysis[J]. Lab Chip, 2012, 12(16):2914-2921.
19. CHEN H H, SHEN H, HEIMFELD S, et al. A microfluidic study of mouse dendritic cell membrane transport properties of water and cryoprotectants[J]. Int J Heat Mass Transf, 2008, 51(23/24):5687-5694.
20. WANG J, ZHU K, ZHAO G, et al. Dual dependence of cryobiogical properties of Sf21 cell membrane on the temperature and the concentration of the cryoprotectant[J]. PLoS One, 2013, 8(9):e72836.
21. HUBEL A. Advancing the preservation of cellular therapy products[J]. Transfusion, 2011, 51(Suppl 4):82S-86S.
22. FLEMING K K, LONGMIRE E K, HUBEL A. Numerical characterization of diffusion-based extraction in cell-laden flow through a microfluidic Channel[J]. J Biomech Eng, 2007, 129(5):703-711.
23. MATA C, LONGMIRE E K, MCKENNA D H, et al. Experimental study of diffusion-based extraction from a cell suspension[J]. Microfluid Nanofluidics, 2008, 5(4):529-540.
24. MATA C, LONGMIRE E, MCKENNA D, et al. Cell motion and recovery in a two-stream microfluidic device[J]. Microfluid Nanofluidics, 2010, 8(4):457-465.
25. HANNA J, HUBEL A, LEMKE E. Diffusion-based extraction of DMSO from a cell suspension in a three stream, vertical microchannel[J]. Biotechnol Bioeng, 2012, 109(9):2316-2324.
26. SONG Y S, MOON S, HULLI L, et al. Microfluidics for cryopreservation[J]. Lab Chip, 2009, 9(13):1874-1881.
27. HEO Y S, LEE H J, HASSELL B A, et al. Controlled loading of cryoprotectants (CPAs) to oocyte with linear and complex CPA profiles on a microfluidic platform[J]. Lab Chip, 2011, 11(20):3530-3537.
28. LI S, LIU W, LIN L. On-Chip cryopreservation of living cells[J]. Journal of the Association for Laboratory Automation, 2010, 15(2):99-106.
29. ZOU Y, YIN T, CHEN S, et al. On-chip cryopreservation:a novel method for ultra-rapid cryoprotectant-free cryopreservation of small amounts of human spermatozoa[J]. PLoS One, 2013, 8(4):e61593.
30. ZHOU X M, LIU Z, LIANG X M, et al. Theoretical investigations of a novel microfluidic cooling/warming system for cell vitrification cryopreservation[J]. Int J Heat Mass Transf, 2013, 65:381-388.

Journal of Biomedical Engineering

Research Progress of Application of Microfluidics Techniques in Cryopreservation

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