Promotion of Function of Endothelial Progenitor Cells with Shexiang Baoxin Pill Treatment under Shear Stress_Journal of Biomedical Engineering

Authors：

LIGang ¹ , CHENYang ¹ ,  WUJiang ^2,3

1. Department of Cardiology, Sichuan Provincial People's Hospital, Chengdu 610072, China;
2. School of West China Basic Medicine and Forensic Medicine, Sichuan University, Chengdu 610041, China;
3. School of Basic Medicine of Xinjiang Medical University, Urumqi 830011, China;

Corresponding author：

WUJiang, Email: 1213365452@qq.com

Keywords：

shear stress; Shexiang Baoxin pill; Endothelial progenitor cell; re-endothelialization

DOI：

10.7507/1001-5515.20150152

Video：

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The aim of this study was to investigate whether shear stress could promote function of endothelial progenitor cells (EPCs)with Shexiang Baoxin Pill (SBP) treatment in vitro, and to study whether shear stress contributed to vascular injury repair by EPCs. EPCs were isolated and characterized; EPCs' proliferation, migration, adhesion, tube formation and eNOS protein level in vitro were investigated by culturing confluent EPCs in 4 mg/mL SBP under physiological shear stress (15 dyne/cm²) for up to 24 hours. Afterwards, EPCs were transfused into rats after wire-induced carotid artery injury augmented re-endothelialization. The results showed that, compared to the SBP group, the shear stress+SBP group obviously enhanced EPCs proliferation, migration, adhesion, tube formation and eNOS protein expression in vitro (P<0.01). After one week, immunofluorescence staining showed that endothelial regeneration rate obviously enhanced in shear stress+SBP group (P<0.01). The present study demonstrates that shear stress can promote function of endothelial progenitor cells treated with SBP, which improves the vascular injury repair potentials of EPCs.

Citation： LIGang, CHENYang, WUJiang. Promotion of Function of Endothelial Progenitor Cells with Shexiang Baoxin Pill Treatment under Shear Stress. Journal of Biomedical Engineering, 2015, 32(4): 847-853. doi: 10.7507/1001-5515.20150152 Copy

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1. ZHAO Y H,YUAN B,CHEN J,et al.Endothelial progenitor cells:therapeutic perspective for ischemic stroke[J].CNS Neurosci Ther,2013,19(2):67-75.
2. JIANG Q J,GONG Z G,DING S F.Endothelial progenitor cells may participate in stress-induced tumour angiogenesis[J].Med Hypotheses,2013,80(6):778-780.
3. HUANG H M,YANG Y G,LV C,et al.Pharmacokinetics and tissue distribution of five bufadienolides from the Shexiang Baoxin pill following oral administration to mice[J].J Ethnopharmacol,2015,161:175-185.
4. REHMAN J,LI J L,ORSCHELL C M,et al.Peripheral blood "endothelial progenitor cells" are derived from monocyte/macrophages and secrete angiogenic growth factors[J].Circulation,2003,107(8):1164-1169.
5. OBI S,MASUDA H,SHIZUNO T,et al.Fluid shear stress induces differentiation of circulating phenotype endothelial progenitor cells[J].Am J Physiol Cell Physiol,2012,303(6):C595-C606.
6. DONG H F,TANG H,CHEN D Z,et al.Analysis of 7 synthetic musks in cream by supported liquid extraction and solid phase extraction followed by GC-MS/MS[J].Talanta,2014,120:248-254.
7. 曹政,谢华强,陈平英,等.麝香保心丸对冠心病患者内皮祖细胞数量及血浆一氧化氮水平的影响[J].中西医结合心脑血管病杂志,2012,10(9):1038-1039.
8. 吴建祥,梁春,任雨笙,等.麝香保心丸对内皮祖细胞功能及分泌一氧化氮的影响[J].中国中西医结合杂志,2009,29(6):511-513.
9. 罗心平,李勇,范维琥,等.麝香保心丸对兔动脉壁一氧化氮代谢影响的研究[J].中国中西医结合杂志,1998,18(S1):36-38,357.
10. YE C,BAI L,YAN Z Q,et al.Shear stress and vascular smooth muscle cells promote endothelial differentiation of endothelial progenitor cells via activation of Akt[J].Clin Biomech (Bristol,Avon),2008,23(Suppl 1):S118-S124.
11. YANG Z,XIA W H,ZHANG Y Y,et al.Shear stress-induced activation of Tie2-dependent signaling pathway enhances reendothelialization capacity of early endothelial progenitor cells[J].J Mol Cell Cardiol,2012,52(5):1155-1163.

Journal of Biomedical Engineering

Promotion of Function of Endothelial Progenitor Cells with Shexiang Baoxin Pill Treatment under Shear Stress

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