Influence of Emodin Gel on the Fibroblasts of Hypertrophic Scars in Rabbit Ear Model_Journal of Biomedical Engineering

Authors：

TAOYingxia ,  QUYun

Sichuan Provincial Key Laboratory of Rehabilitation Medicine， Department of Rehabilitation Medicine, West China Hospital, Sichuan University, Chengdu 610041, China;

Corresponding author：

QUYun, Email: quyben@163.com

Keywords：

hypertrophic scar; Emodin; rabbit

DOI：

10.7507/1001-5515.20150154

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This research was aimed to study the effect of Emodin gel on the hypertrophic scars of rabbit ears. A total of 18 rabbits were randomly divided into Emodin group (9 rabbits) and control group (9 rabbits) after the successful animal model for hypertrophic scars had been made. The rabbits in the Emodin group were treated with Emodin Gel,while no special treatment was given to those in the control group. The other living conditions were all kept the same in the two groups. The diameter,hardness, and expression of transforming growth factor-β (TGF-β) and interleukin-1 (IL-1) of hypertrophic scars were measured after 4 weeks. Transmission electron microscopy was applied to observe the ultra-structure of the fibroblasts of hypertrophic scars. But there was no difference between the two groups in the diameter of hypertrophic scars (P>0.05). The hardness, expression of TGF-β and IL-1 in hypertrophic scars in the Emodin group decreased, compared to the control group (P<0.05, P<0.01, P<0.05). Transmission electron microscopy showed that the fibroblast and organelle lessened in the cytoplasm and the collagen fibers dissolved obviously. The study showed that Emodin gel decreased the hardness of hypertrophic scars in the rabbit ears, and inhibited the proliferation of fibroblasts in local area. Therefore, Emodin gel treatment would be one of the methods to prevent and treat hypertrophic scars.

Citation： TAOYingxia, QUYun. Influence of Emodin Gel on the Fibroblasts of Hypertrophic Scars in Rabbit Ear Model. Journal of Biomedical Engineering, 2015, 32(4): 862-866. doi: 10.7507/1001-5515.20150154 Copy

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16.	TANDARA A A,KLOETERS O,MOGFORD J E,et al.Hydrated keratinocytes reduce collagen synthesis by fibroblasts via paracrine mechanisms[J].Wound Repair Regen,2007,15(4):497-504.
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19.	GALLANT-BEHM C L,MUSTOE T A.Occlusion regulates epidermal cytokine production and inhibits scar formation[J].Wound Repair Regen,2010,18(2):235-244.

1. JUCKETT G,HARTMAN-ADAMS H.Management of keloids and hypertrophic scars[J].Am Fam Physician,2009,80(3):253-260.
2. GAUGLITZ,G G.Management of keloids and hypertrophic scars:current and emerging options[J].Clin Cosmet Investig Dermatol,2013,6:103-114.
3. MORRIS D E,WU L,ZHAO L L,et al.Acute and chronic animal models for excessive dermal scarring:quantitative studies[J].Plast Reconstr Surg,1997,100(3):674-681.
4. 李荟元,刘建波,夏炜,等.增生性瘢痕动物实验模型的建立与应用[J].中华整形外科杂志,2001,17(5):276-278.
5. 李荟元.增生性瘢痕动物实验模型应用最新动态[J].中国美容医学,2013,22(13):1455-1461.
6. REN H T,HU H,LI Y,et al.Endostatin inhibits hypertrophic scarring in a rabbit ear model[J].J Zhejiang Univ Sci B,2013,14(3):224-230.
7. TOLLEFSON T T,KAMANGAR F,AMINPOUR S,et al.Comparison of effectiveness of silicone gel sheeting with microporous paper tape in the prevention of hypertrophic scarring in a rabbit model[J].Arch Facial Plast Surg,2012,14(1):45-51.
8. 屈云,姚平,李廷谦,等.大黄素对肺成纤维细胞增殖及细胞周期的影响[J].四川大学学报(医学版),2004,35(1):74-76.
9. 秦建华,陈明.大黄素抗肾间质纤维化研究进展[J].中国中西医结合肾病杂志,2006,7(3):84-186.
10. 夏珊,李世荣,刘剑毅,等.大黄素对人增生性瘢痕成纤维细胞的增殖抑制及细胞内游离Ca²⁺的作用[J].第三军医大学学报,2007,29(20):1982-1984.
11. WANG Q,ZHANG N N,LI H Y,et al.Active ingredients in rhubarb with anti-proliferative effects on scar fibroblasts[J].Yao Xue Xue Bao,2012,47(12):1618-1622.
12. POLO M,KO F,BUSILLO F,et al.Cytokine production in patients with hypertrophic burn scars[J].J Burn Care Rehabil,1997,18(6):477-482.
13. WANG J,JIAO H,STEWART T L,et al.Increased TGF-beta-producing CD4+T lymphocytes in postburn patients and their potential interaction with dermal fibroblasts in hypertrophic scarring[J].Wound Repair Regen,2007,15(4):530-539.
14. NOWAK K C,MCCORMACK M,KOCH R J.The effect of superpulsed carbon dioxide laser energy on keloid and normal dermal fibroblast secretion of growth factors:a serum-free study[J].Plast Reconstr Surg,2000,105(6):2039-2048.
15. BARROW R E,DASU M R.Oxidative and heat stress gene changes in hypertrophic scar fibroblasts stimulated with interleukin-1beta[J].J Surg Res,2005,126(1):59-65.
16. TANDARA A A,KLOETERS O,MOGFORD J E,et al.Hydrated keratinocytes reduce collagen synthesis by fibroblasts via paracrine mechanisms[J].Wound Repair Regen,2007,15(4):497-504.
17. EROL O O,GURLEK A,AGAOGLU G,et al.Treatment of hypertrophic scars and keloids using intense pulsed light (IPL)[J].Aesthetic Plast Surg,2008,32(6):902-909.
18. AKAISHI S,KOIKE S,DOHI T,et al.Nd:YAG Laser Treatment of Keloids and Hypertrophic Scars[J].Eplasty,2012,12:e1.
19. GALLANT-BEHM C L,MUSTOE T A.Occlusion regulates epidermal cytokine production and inhibits scar formation[J].Wound Repair Regen,2010,18(2):235-244.

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