Effect of Conditioned Medium from Endothelial Cells on Cancer Stem Cell Phenotype of Hepatoma Cells_Journal of Biomedical Engineering

Authors：

FENGChuan ¹ , YANGXianjiong ^1,2 , SUNJinghui ¹ , LUOQing ¹ ,  SONGGuanbin ¹

1. College of Bioengineering, Chongqing University, Chongqing 400044, China;
2. Department of Chemistry, Guiyang Medical University, Guiyang 550004, China;

Corresponding author：

SONGGuanbin, Email: song9973@126.com

Keywords：

endothelial cell; conditioned medium; hepatoma cells; cancer stem cell; phenotype; co-culture

DOI：

10.7507/1001-5515.20150188

Video：

Export PDF Favorites Scan Get Citation

Abstract Full text Figures/Tables Video References Cited by

In this study, we aimed to investigate the influences of conditioned medium from human umbilical vein endothelial cells (HUVEC) on cancer stem cell phenotype of human hepatoma cells. HUVEC and human hepatoma cells (MHCC97H) were cultured, respectively, and then the MHCC97H cells were co-cultured with conditioned medium from HUVEC (EC-CM) with Transwell system. Anti-cancer drug sensitivity, colony-formation, migration/invasion ability, expression of cancer stem cell marker and sphere formation were performed to determine the cancer stem cell phenotype in MHCC97H cells. We found that MHCC97H cells co-cultured with EC-CM exhibited significantly higher colony-formation ability and lower sensitivity of anti-cancer drugs 5-FU and Cis. Transwell assay showed that treatment with EC-CM obviously increased migration and invasion of MHCC97H cells. Moreover, increased sphere forming capability and expression of CD133 in MHCC97H cells were observed after co-cultured with EC-CM. These results suggested that EC-CM could promote cancer stem cell phenotype of hepatoma cells.

Citation： FENGChuan, YANGXianjiong, SUNJinghui, LUOQing, SONGGuanbin. Effect of Conditioned Medium from Endothelial Cells on Cancer Stem Cell Phenotype of Hepatoma Cells. Journal of Biomedical Engineering, 2015, 32(5): 1061-1066. doi: 10.7507/1001-5515.20150188 Copy

1.	VINEIS P, WILD C P. Global cancer patterns:causes and prevention[J]. Lancet, 2014, 383(9916):549-557.
2.	CAO Lu, ZHOU Yanming, ZHAI Beibei, et al. Sphere-forming cell subpopulations with cancer stem cell properties in human hepatoma cell lines[J]. BMC Gastroenterol, 2011, 11:71-81.
3.	MATAR P, ALANIZ L, ROZADOS V, et al. Immunotherapy for liver tumors:present status and future prospects[J]. J Biomed Sci, 2009, 16:27-30.
4.	YAMASHITA T, JI Junfang, BUDHU A, et al. EpCAM-positive hepatocellular carcinoma cells are tumor-initiating cells with stem/progenitor cell features[J]. Gastroenterology, 2009, 136(3):1012-1024.
5.	LEE T K W, CASTILHO A, CHEUNG V C H, et al. CD24+ liver tumor-initiating cells drive self-renewal and tumor initiation through STAT3-mediated NANOG regulation[J]. Cell Stem Cell, 2011, 9(1):50-63.
6.	CALABRESE C, POPPLETON H, KOCAK M, et al. A perivascular niche for brain tumor stem cells[J]. Cancer Cell, 2007, 11(1):69-82.
7.	KRISHNAMURTHY S, DONG Zhihong, VODOPYANOV D, et al. Endothelial cell-initiated signaling promotes the survival and self-renewal of cancer stem cells[J]. Cancer Res, 2010, 70(23):9969-9978.
8.	CHENG Lin, HUANG Zhi, ZHOU Wenchao, et al. Glioblastoma stem cells generate vascular pericytes to support vessel function and tumor growth[J]. Cell, 2013, 153(1):139-152.
9.	LU Jia, YE Xiang-cang, FAN Fan, et al. Endothelial cells promote the colorectal cancer stem cell phenotype through a soluble form of jagged-1[J]. Cancer Cell, 2013, 23(2):171-185.
10.	CAMPOS M S, NEIVA K G, MEYERS K A, et al. Endothelial derived factors inhibit anoikis of head and neck cancer stem cells[J]. Oral Oncol, 2012, 48(1):26-32.
11.	MARQUARDT J U, GALLE P R, TEUFEL A. Molecular diagnosis and therapy of hepatocellular carcinoma (HCC):an emerging field for advanced technologies[J]. J Hepatol, 2012, 56(1):267-275.
12.	MA S. Biology and clinical implications of CD133+ liver cancer stem cells[J]. Exp Cell Res, 2013, 319(2):126-132.
13.	YANG Zhen-fan, HO D W, NG M N, et al. Significance of CD90+ cancer stem cells in human liver cancer[J]. Cancer Cell, 2008, 13(2):153-166.
14.	PIAO Lian-shu, HUR W, KIM T K, et al. CD133+ liver cancer stem cells modulate radioresistance in human hepatocellular carcinoma[J]. Cancer Lett, 2012, 315(2):129-137.
15.	MA S, TANG K H, CHAN Y P, et al. miR-130b promotes CD133+ liver tumor-initiating cell growth and self-renewal via tumor protein 53-induced nuclear protein 1[J]. Cell Stem Cell, 2010, 7(6):694-707.
16.	CHOI S A, WANG K C, PHI J H, et al. A distinct subpopulation within CD133 positive brain tumor cells shares characteristics with endothelial progenitor cells[J]. Cancer Lett, 2012, 324(2):221-230.
17.	WU Qiuhua, GUO Rongjiao, LIN Min, et al. MicroRNA-200a inhibits CD133/1+ ovarian cancer stem cells migration and invasion by targeting E-cadherin repressor ZEB2[J]. Gynecol Oncol, 2011, 122(1):149-154.
18.	OSKARSSON T, BATLLE E, MASSAGUE J. Metastatic stem cells:sources, niches, and vital pathways[J]. Cell Stem Cell, 2014, 14(3):306-321.
19.	ZHU T S, COSTELLO M A, TALSMA C E, et al. Endothelial cells create a stem cell niche in glioblastoma by providing NOTCH ligands that nurture self-renewal of cancer stem-like cells[J]. Cancer Res, 2011, 71(18):6061-6072.
20.	ZHANG Zhaocheng, DONG Zhihong, LAUXEN I S, et al. Endothelial cell-secreted EGF induces epithelial to mesenchymal transition and endows head and neck cancer cells with stem-like phenotype[J]. Cancer Res, 2014, 74(10):2869-2881.

1. VINEIS P, WILD C P. Global cancer patterns:causes and prevention[J]. Lancet, 2014, 383(9916):549-557.
2. CAO Lu, ZHOU Yanming, ZHAI Beibei, et al. Sphere-forming cell subpopulations with cancer stem cell properties in human hepatoma cell lines[J]. BMC Gastroenterol, 2011, 11:71-81.
3. MATAR P, ALANIZ L, ROZADOS V, et al. Immunotherapy for liver tumors:present status and future prospects[J]. J Biomed Sci, 2009, 16:27-30.
4. YAMASHITA T, JI Junfang, BUDHU A, et al. EpCAM-positive hepatocellular carcinoma cells are tumor-initiating cells with stem/progenitor cell features[J]. Gastroenterology, 2009, 136(3):1012-1024.
5. LEE T K W, CASTILHO A, CHEUNG V C H, et al. CD24+ liver tumor-initiating cells drive self-renewal and tumor initiation through STAT3-mediated NANOG regulation[J]. Cell Stem Cell, 2011, 9(1):50-63.
6. CALABRESE C, POPPLETON H, KOCAK M, et al. A perivascular niche for brain tumor stem cells[J]. Cancer Cell, 2007, 11(1):69-82.
7. KRISHNAMURTHY S, DONG Zhihong, VODOPYANOV D, et al. Endothelial cell-initiated signaling promotes the survival and self-renewal of cancer stem cells[J]. Cancer Res, 2010, 70(23):9969-9978.
8. CHENG Lin, HUANG Zhi, ZHOU Wenchao, et al. Glioblastoma stem cells generate vascular pericytes to support vessel function and tumor growth[J]. Cell, 2013, 153(1):139-152.
9. LU Jia, YE Xiang-cang, FAN Fan, et al. Endothelial cells promote the colorectal cancer stem cell phenotype through a soluble form of jagged-1[J]. Cancer Cell, 2013, 23(2):171-185.
10. CAMPOS M S, NEIVA K G, MEYERS K A, et al. Endothelial derived factors inhibit anoikis of head and neck cancer stem cells[J]. Oral Oncol, 2012, 48(1):26-32.
11. MARQUARDT J U, GALLE P R, TEUFEL A. Molecular diagnosis and therapy of hepatocellular carcinoma (HCC):an emerging field for advanced technologies[J]. J Hepatol, 2012, 56(1):267-275.
12. MA S. Biology and clinical implications of CD133+ liver cancer stem cells[J]. Exp Cell Res, 2013, 319(2):126-132.
13. YANG Zhen-fan, HO D W, NG M N, et al. Significance of CD90+ cancer stem cells in human liver cancer[J]. Cancer Cell, 2008, 13(2):153-166.
14. PIAO Lian-shu, HUR W, KIM T K, et al. CD133+ liver cancer stem cells modulate radioresistance in human hepatocellular carcinoma[J]. Cancer Lett, 2012, 315(2):129-137.
15. MA S, TANG K H, CHAN Y P, et al. miR-130b promotes CD133+ liver tumor-initiating cell growth and self-renewal via tumor protein 53-induced nuclear protein 1[J]. Cell Stem Cell, 2010, 7(6):694-707.
16. CHOI S A, WANG K C, PHI J H, et al. A distinct subpopulation within CD133 positive brain tumor cells shares characteristics with endothelial progenitor cells[J]. Cancer Lett, 2012, 324(2):221-230.
17. WU Qiuhua, GUO Rongjiao, LIN Min, et al. MicroRNA-200a inhibits CD133/1+ ovarian cancer stem cells migration and invasion by targeting E-cadherin repressor ZEB2[J]. Gynecol Oncol, 2011, 122(1):149-154.
18. OSKARSSON T, BATLLE E, MASSAGUE J. Metastatic stem cells:sources, niches, and vital pathways[J]. Cell Stem Cell, 2014, 14(3):306-321.
19. ZHU T S, COSTELLO M A, TALSMA C E, et al. Endothelial cells create a stem cell niche in glioblastoma by providing NOTCH ligands that nurture self-renewal of cancer stem-like cells[J]. Cancer Res, 2011, 71(18):6061-6072.
20. ZHANG Zhaocheng, DONG Zhihong, LAUXEN I S, et al. Endothelial cell-secreted EGF induces epithelial to mesenchymal transition and endows head and neck cancer cells with stem-like phenotype[J]. Cancer Res, 2014, 74(10):2869-2881.

Previous Article
Promotion of Pink1S Auto-phosphorylation with CK2β
Next Article
Molecular Image of Superparamagnetic Iron Oxide Nanopariticle Labeled with hATF in Colon Tumor Models

Journal of Biomedical Engineering

Effect of Conditioned Medium from Endothelial Cells on Cancer Stem Cell Phenotype of Hepatoma Cells

Abstract Full text Figures/Tables Video References Cited by

Previous Article

Next Article

Format

Content