Expression of MicroRNAs of An Interneuron Precursor Cell Line GE6 in Various Differentiation Conditions_Journal of Biomedical Engineering

Authors：

GEXinxu ¹ , LIUQian ¹ , YINShu ² ,  LIHedong ¹

1. Development and Stem Cell Institute, West China Second University Hospital, Sichuan University, Chengdu 610041, China;
2. Institute of Neuroscience, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai 200031, China;

Corresponding author：

LIHedong, Email: hedongli2009@hotmail.com

Keywords：

microRNA; microRNA chip; neural progenitor; interneuron; differentiation

DOI：

10.7507/1001-5515.20150226

Video：

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Abstract Full text Figures/Tables Video References Cited by

The purpose of this study was to identify specific microRNAs (miRNAs) during differentiation and maturation of interneurons and to predict their possible functions by analyzing the expression of miRNAs during in vitro differentiation of the rat interneuron precursor cell line GE6. In the experiment, the interneuron precursor cell line GE6 was cultured under three different conditions, i.e. the first was that had not added growth factors and the normal differentiation cultured for 4 days (Ge6_4d); the second was that cultured with bone morphogenetic protein-2 (BMP2) for 4 days (Ge6_bmp2); and the third was that cultured with sonic hedgehog (SHH) for 4 days (Ge6_shh). In addition, another group of undifferentiated GE6 (Ge6_u) was applied as a control. We found in this study that the expression levels of a large number of miRNAs changed significantly during GE6 differentiation. The expression levels of miR-710, miR-290-5p and miR-3473 increased in the GE6 cells with secreted factor BMP2. These miRNAs may play important regulatory roles during interneuron differentiation.

Citation： GEXinxu, LIUQian, YINShu, LIHedong. Expression of MicroRNAs of An Interneuron Precursor Cell Line GE6 in Various Differentiation Conditions. Journal of Biomedical Engineering, 2015, 32(6): 1273-1278. doi: 10.7507/1001-5515.20150226 Copy

1.	MARIN O, ANDERSON S A, RUBENSTEIN J L. Origin and molecular specification of striatal interneurons[J]. J Neurosci, 2000, 20(16):6063-6076.
2.	XU Qing, COBOS I, DE LA CRUZ E, et al. Origins of cortical interneuron subtypes[J]. J Neurosci, 2004, 24(11):2612-2622.
3.	ANDERSON S A, EISENSTAT D D, SHI L, et al. Interneuron migration from basal forebrain to neocortex:dependence on Dlx genes[J]. Science, 1997, 278(5337):474-476.
4.	ANDERSON S A, MARÍN O, HORN C, et al. Distinct cortical migrations from the medial and lateral ganglionic eminences[J]. Development, 2001, 128(3):353-363.
5.	PLEASURE S J, ANDERSON S, HEVNER R, et al. Cell migration from the ganglionic eminences is required for the development of hippocampal GABAergic interneurons[J]. Neuron, 2000, 28(3):727-740.
6.	FRITSCH B, QASHU F, FIGUEIREDO T H, et al. Pathological alterations in GABAergic interneurons and reduced tonic inhibition in the basolateral amygdala during epileptogenesis[J]. Neuroscience, 2009, 163(1):415-429.
7.	DE PIETRI TONELLI D, PULVERS J N, HAFFNER C, et al. miRNAs are essential for survival and differentiation of newborn neurons but not for expansion of neural progenitors during early neurogenesis in the mouse embryonic neocortex[J]. Development, 2008, 135(23):3911-3921.
8.	LI Hedong, HADER A T, HAN Y R, et al. Isolation of a novel rat neural progenitor clone that expresses Dlx family transcription factors and gives rise to functional GABAergic neurons in culture[J]. Dev Neurobiol, 2012, 72(6):805-820.
9.	QURESHI I A, MEHLER M F. Epigenetic mechanisms underlying human epileptic disorders and the process of epileptogenesis[J]. Neurobiol Dis, 2010, 39(1):53-60.
10.	BUTT S J, SOUSA V H, FUCCILLO M V, et al. The requirement of Nkx2-1 in the temporal specification of cortical interneuron subtypes[J]. Neuron, 2008, 59(5):722-732.
11.	XU Qing, GUO Lihua, MOORE H, et al. Sonic hedgehog signaling confers ventral telencephalic progenitors with distinct cortical interneuron fates[J]. Neuron, 2010, 65(3):328-340.
12.	SAMANTA J, BURKE G M, MCGUIRE T, et al. BMPR1a signaling determines numbers of oligodendrocytes and calbindin-expressing interneurons in the cortex[J]. J Neurosci, 2007, 27(28):7397-7407.
13.	WINE-LEE L, AHN K J, RICHARDSON R D, et al. Signaling through BMP type 1 receptors is required for development of interneuron cell types in the dorsal spinal cord[J]. Development, 2004, 131(21):5393-5403.
14.	GARCIA P B, CAI Amie, BATES J G, et al. miR290-5p/292-5p activate the immunoglobulin kappa locus in B cell development[J]. PLoS One, 2012, 7(8):e43805.
15.	LEONE V, D'ANGELO D, FERRARO A, et al. A TSH-CREB1-microRNA loop is required for thyroid cell growth[J]. Mol Endocrinol, 2011, 25(10):1819-1830.
16.	CHAVELES I, ZARAVINOS A, HABEOS I G, et al. MicroRNA profiling in murine liver after partial hepatectomy[J]. Int J Mol Med, 2012, 29(5):747-755.
17.	GOLDBERGER N, WALKER R C, KIM C H, et al. Inherited variation in miR-290 expression suppresses breast cancer progression by targeting the metastasis susceptibility gene Arid4b[J]. Cancer Res, 2013, 73(8):2671-2681.
18.	EDINGER R S, CORONNELLO C, BODNAR A J, et al. Aldosterone regulates microRNAs in the cortical collecting duct to alter Sodium transport[J]. J Am Soc Nephrol, 2014, 25(11):2445-2457.

1. MARIN O, ANDERSON S A, RUBENSTEIN J L. Origin and molecular specification of striatal interneurons[J]. J Neurosci, 2000, 20(16):6063-6076.
2. XU Qing, COBOS I, DE LA CRUZ E, et al. Origins of cortical interneuron subtypes[J]. J Neurosci, 2004, 24(11):2612-2622.
3. ANDERSON S A, EISENSTAT D D, SHI L, et al. Interneuron migration from basal forebrain to neocortex:dependence on Dlx genes[J]. Science, 1997, 278(5337):474-476.
4. ANDERSON S A, MARÍN O, HORN C, et al. Distinct cortical migrations from the medial and lateral ganglionic eminences[J]. Development, 2001, 128(3):353-363.
5. PLEASURE S J, ANDERSON S, HEVNER R, et al. Cell migration from the ganglionic eminences is required for the development of hippocampal GABAergic interneurons[J]. Neuron, 2000, 28(3):727-740.
6. FRITSCH B, QASHU F, FIGUEIREDO T H, et al. Pathological alterations in GABAergic interneurons and reduced tonic inhibition in the basolateral amygdala during epileptogenesis[J]. Neuroscience, 2009, 163(1):415-429.
7. DE PIETRI TONELLI D, PULVERS J N, HAFFNER C, et al. miRNAs are essential for survival and differentiation of newborn neurons but not for expansion of neural progenitors during early neurogenesis in the mouse embryonic neocortex[J]. Development, 2008, 135(23):3911-3921.
8. LI Hedong, HADER A T, HAN Y R, et al. Isolation of a novel rat neural progenitor clone that expresses Dlx family transcription factors and gives rise to functional GABAergic neurons in culture[J]. Dev Neurobiol, 2012, 72(6):805-820.
9. QURESHI I A, MEHLER M F. Epigenetic mechanisms underlying human epileptic disorders and the process of epileptogenesis[J]. Neurobiol Dis, 2010, 39(1):53-60.
10. BUTT S J, SOUSA V H, FUCCILLO M V, et al. The requirement of Nkx2-1 in the temporal specification of cortical interneuron subtypes[J]. Neuron, 2008, 59(5):722-732.
11. XU Qing, GUO Lihua, MOORE H, et al. Sonic hedgehog signaling confers ventral telencephalic progenitors with distinct cortical interneuron fates[J]. Neuron, 2010, 65(3):328-340.
12. SAMANTA J, BURKE G M, MCGUIRE T, et al. BMPR1a signaling determines numbers of oligodendrocytes and calbindin-expressing interneurons in the cortex[J]. J Neurosci, 2007, 27(28):7397-7407.
13. WINE-LEE L, AHN K J, RICHARDSON R D, et al. Signaling through BMP type 1 receptors is required for development of interneuron cell types in the dorsal spinal cord[J]. Development, 2004, 131(21):5393-5403.
14. GARCIA P B, CAI Amie, BATES J G, et al. miR290-5p/292-5p activate the immunoglobulin kappa locus in B cell development[J]. PLoS One, 2012, 7(8):e43805.
15. LEONE V, D'ANGELO D, FERRARO A, et al. A TSH-CREB1-microRNA loop is required for thyroid cell growth[J]. Mol Endocrinol, 2011, 25(10):1819-1830.
16. CHAVELES I, ZARAVINOS A, HABEOS I G, et al. MicroRNA profiling in murine liver after partial hepatectomy[J]. Int J Mol Med, 2012, 29(5):747-755.
17. GOLDBERGER N, WALKER R C, KIM C H, et al. Inherited variation in miR-290 expression suppresses breast cancer progression by targeting the metastasis susceptibility gene Arid4b[J]. Cancer Res, 2013, 73(8):2671-2681.
18. EDINGER R S, CORONNELLO C, BODNAR A J, et al. Aldosterone regulates microRNAs in the cortical collecting duct to alter Sodium transport[J]. J Am Soc Nephrol, 2014, 25(11):2445-2457.

Journal of Biomedical Engineering

Expression of MicroRNAs of An Interneuron Precursor Cell Line GE6 in Various Differentiation Conditions

Abstract Full text Figures/Tables Video References Cited by

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