Screening the Optimal Time of Sinusoidal Alternating Electromagnetic Field for the Bone Biomechanical Properties of Rat_Journal of Biomedical Engineering

Authors：

GAOYuhai , LIShaofeng , ZHOUYanfeng , ZHOUJian , SHIWengui , XIEYanfang , FANGQingqing , RENQian ,  CHENKeming

Institute of Orthopaedics, Lanzhou General Hospital of PLA, Lanzhou 730050, China;

Corresponding author：

CHENKeming, Email: Chenkeming@126.com

Keywords：

sinusoidal electromagnetic fields; bone mineral density; bone biomechanics

DOI：

10.7507/1001-5515.20160087

Video：

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The present research is to investigate the time effect of sinusoidal electromagnetic fields (SEMFs) at different exposure time on the biomechanical properties in rats, and to find a best time for improving biomechanical properties. Forty female SD rats were randomly divided into five groups, i.e. control group, 45 min SEMFs group, 90 min SEMFs group, 180 min SEMFs group, and 270 min SEMFs group. In addition to the control group, other groups were exposed to 50 Hz and 0.1 mT magnetic field every day for the corresponding time periods. After eight weeks, bone mineral density (BMD), bone biomechanics, bone tissue morphology, micro-CT and pathological examination were performed. The results showed that there was no abnormal pathological finding in the experimental groups. In the 90 min SEMFs group, BMD, femur maximum load, elastic modulus, yield strength, trabecular number (Tb.N), trabecular thickness (Tb.Th) and trabecular area (Tb.Ar) percentage were all significantly higher than those in the control group (P＜0.01), and trabecular separation (Tb.Sp) was significantly lower than that of the control group (P＜0.01). However, for other experimental groups, some indices showed statistical significance compared to the control group (P＜0.05), but some did not (P>0.05). This study showed that under 50 Hz and 0.1 mT SEMFs, 90 min is the best time that can effectively increase bone mineral density, improve the bone tissue microstructure organization and the biomechanical properties.

Citation： GAOYuhai, LIShaofeng, ZHOUYanfeng, ZHOUJian, SHIWengui, XIEYanfang, FANGQingqing, RENQian, CHENKeming. Screening the Optimal Time of Sinusoidal Alternating Electromagnetic Field for the Bone Biomechanical Properties of Rat. Journal of Biomedical Engineering, 2016, 33(3): 520-525. doi: 10.7507/1001-5515.20160087 Copy

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10.	LAFFOSSE J M, KINKPE C, GOMEZ-BROUCHET A, et al. Micro-computed tomography study of the subchondral bone of the vertebral endplates in a porcine model:correlations with histomorphometric parameters[J]. Surg Radiol Anat, 2010, 32(4):335-341.
11.	BREDELLA M A, LIN E, GERWECK A V, et al. Determinants of bone microarchitecture and mechanical properties in obese men[J]. J Clin Endocrinol Metab, 2012, 97(11):4115-4122.
12.	陈文双,许碧莲,崔燎,等.大鼠椎骨生物力学与骨组织形态计量学的灰色关联分析[J].数理医药学杂志,2011,24(1):38-41.

1. CRANNEY A, GUYATT G, GRIFFITH L, et al; Osteoporosis Methodology Group and The Osteoporosis Research Advisory Group. Meta-analyses of therapies for postmenopausal osteoporosis. Ⅲ:Summary of meta-analyses of therapies for postmenopausal osteoporosis[J]. Endocr Rev, 2002; 23(4):570-578.
2. XU J, RONG H, JI H, et al. Effects of different dosages of parathyroid hormone-related protein 1-34 on the bone metabolism of the ovariectomized rat model of osteoporosis[J]. Calcif Tissue Int, 2013, 93(3):276-287.
3. 姚新苗,徐禄基,冷涛,等.益骨口服液对去势大鼠骨密度与骨生物力学的影响[J].浙江中医药大学学报,2010,34(2):142-143.
4. BREDARIOLI M, DALIO M B, JOVILIANO E E, et al. Effect of cilostazol and pentoxifylline on gait biomechanics in rats with ischemic left hindlimb[J]. J Vasc Surg, 2012, 56(2):476-481.
5. TABRAH F, HOFFMEIER M, GILBERT F, et al. Bone density changes in osteoporosis-prone women exposed to pulsed electromagnetic fields (PEMFs)[J]. J Bone Miner Res, 1990, 5(5):437-442.
6. CHENG Guozheng, ZHAI Yuankun, CHEN Keming, et al. Sinusoidal electromagnetic field stimulates rat osteoblast differentiation and maturation via activation of NO-cGMP-PKG pathway[J]. Nitric Oxide, 2011, 25(3):316-325.
7. 高玉海,成魁,葛宝丰,等.50 Hz不同强度SEMFs对大鼠峰值骨量的影响[J].生物医学工程学杂志,2015,32(1):116-119,136.
8. LANE N E. Epidemiology, etiology, and diagnosis of osteoporosis[J]. Am J Obstet Gynecol, 2006, 194(2 Suppl):S3-S11.
9. FU X, MA X, LU H, et al. Associations of fat mass and fat distribution with bone mineral density in pre- and postmenopausal Chinese women[J]. Osteoporos Int, 2011, 22(1):113-119.
10. LAFFOSSE J M, KINKPE C, GOMEZ-BROUCHET A, et al. Micro-computed tomography study of the subchondral bone of the vertebral endplates in a porcine model:correlations with histomorphometric parameters[J]. Surg Radiol Anat, 2010, 32(4):335-341.
11. BREDELLA M A, LIN E, GERWECK A V, et al. Determinants of bone microarchitecture and mechanical properties in obese men[J]. J Clin Endocrinol Metab, 2012, 97(11):4115-4122.
12. 陈文双,许碧莲,崔燎,等.大鼠椎骨生物力学与骨组织形态计量学的灰色关联分析[J].数理医药学杂志,2011,24(1):38-41.

Journal of Biomedical Engineering

Screening the Optimal Time of Sinusoidal Alternating Electromagnetic Field for the Bone Biomechanical Properties of Rat

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