Preparation and Characterization of Chitosan-Poloxamer-based Antibacterial Hydrogel Containing Silver Nanoparticles_Journal of Biomedical Engineering

Authors：

LIDaheng ¹ ,  YUXuefei ¹ , HUYunrui ² , XITingfei ^3,4 , CHENJia ^5,6 , ZHANGZhixiong ⁴

1. School of Biomedical Engineering, Southern Medical University, Guangzhou 510515, China;
2. School of Pharmaceutical Sciences, Southern Medical University, Guangzhou 510515, China;
3. Center for Biomedical Materials and Tissue Engineering, Academy for Advanced Interdisciplinary Studies, Peking University, Beijing 100871, China;
4. Shenzhen Graduate School, Peking University, Shenzhen 518055, China;
5. Postdoctoral Scientific Research Station in Guangzhou Nansha Information Technology Park Limited Company, Guangzhou 511458, China;
6. Tairan Biotechnology Limited Company, Guangzhou 511458, China;

Corresponding author：

YUXuefei, Email: xuefeiyu@smu.edu.cn

Keywords：

silver nanoparticles; antibacterial hydrogel; biocompatible property

DOI：

10.7507/1001-5515.20160179

Video：

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In order to solve the problem of high cytotoxicity in vitro of nano-silver antibacterial gel, and the problem of large nano-silver particle size and size distribution, this study prepared nano-silver antibacterial gel with better biocompatibility and good antibacterial effect by using physical cross-linking method and using poloxamer as dispersant when prepared nano-silver. In this study, nano-silver was prepared by photo-initiator method and by adding poloxamer as a dispersant, and then UV-visible absorption spectrum test and scanning electron microscopy (SEM) test were carried out using prepared nano-silver mixture and particles after drying respectively. The gel was prepared through adjusting its pH value by using sodium bicarbonate, and then pH value test, SEM test for cross-section of gel, swelling ratio test, viscosity test, inhibition zone test and in vitro cytotoxicity test were carried out. The test results showed that the maximum absorption wavelength of prepared nano-silver, using poloxamer as dispersant and ultra-pure water as solvent, was 414 nm, and the average nano-silver size was about 60 nm. The prepared nano-silver using poloxamer as dispersant had smaller particle diameter and narrower particle size distribution than those using PVP as dispersant. Similarly, the prepared nano-silver using ultra-pure water as solvent also had smaller particle diameter and narrower particle size distribution than those using distilled water as solvent. The pH value of the prepared gel was between 5.8~6.1. The dried gel section had many holes. The water absorption of gel was fine and the viscosity of gel was fit to coat on the gauze. In addition, the prepared gel with nano-silver had greater ability to inhibit Escherichia coli and Staphyloccocus aureus at the concentrations of 24, 18 and 12 μg/mL. And the biocompatibility of the prepared gel with nano-silver was good when the concentration below 24 μg/mL. Based on the above features, the nano-silver antibacterial gel could be used in the treatment of burn or other wounds.

Citation： LIDaheng, YUXuefei, HUYunrui, XITingfei, CHENJia, ZHANGZhixiong. Preparation and Characterization of Chitosan-Poloxamer-based Antibacterial Hydrogel Containing Silver Nanoparticles. Journal of Biomedical Engineering, 2016, 33(6): 1124-1132. doi: 10.7507/1001-5515.20160179 Copy

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1. 李明春, 徐舜,马守栋,等.纳米银凝胶对大鼠烫伤的治疗作用[J].中国医院药学杂志,2012,32(15):1183-1186.
2. 郈秀菊. 妇科外用纳米银抗菌凝胶的研制[D].济南:山东中医药大学,2006.http://cdmd.cnki.com.cn/Article/CDMD-10441-2007217451.htm.
3. 王芳, 毛熙光.纳米银材料在妇科疾病中的应用[J].中国组织工程研究与临床康复,2011,15(16):2993-2996.
4. 黄清泉, 王蓉蓉,王春仁,等.纳米银医疗产品的体外细胞毒性比较[J].药物分析杂志,2009,29(12):2150-2153.
5. 付海洋, 许建霞,徐丽明,等.含纳米银医疗器械的体外细胞毒性试验[J].药物分析杂志,2010,30(7):1354-1356.
6. 熊玲, 蒋学华,陈亮,等.不同粒径银粒子的体外细胞毒性比较[J].中国生物医学工程学报,2007,26(4):600-604.
7. 赵广建. 几种皮肤生物敷料的生物相容性评价[J].中国组织工程研究与临床康复,2008,12(14):2701-2704.
8. 李凯. 不同浓度透明质酸水凝胶性能表征及其对骨髓间充质干细胞三维培养生长行为的影响[D].北京:北京协和医学院,2013.http://cdmd.cnki.com.cn/Article/CDMD-10023-1013311405.htm.
9. 郑连英, 朱江峰,孙昆山.壳聚糖的抗菌性能研究[J].材料科学与工程,2000,18(2):22-24.
10. 程志, 杜娜.壳聚糖在医药领域应用研究进展[J].人民军医,2013,56(2):223-224.
11. 江磊, 林宝凤,梁兴泉,等.壳聚糖及其衍生物水凝胶的研究进展[J].化学通报:印刷版,2007,70(1):47-51.
12. MAYOL L, DE STEFANO D, CAMPANI V, et al. Design and characterization of a chitosan physical gel promoting wound healing in mice[J]. J Mater Sci Mater Med, 2014, 25(6): 1483-1493.
13. 樊新, 黄可龙,刘素琴,等.化学还原法制备纳米银粒子及其表征[J].功能材料,2007,38(6):996-999, 1002.
14. 郭炎荣. 眼用凝胶剂临床应用概述[J].海峡药学,2008,20(4):74-77.
15. 和俊, 司民真.纳米银的制备及其应用[J].光散射学报,2008,20(1):42-46.
16. 唐奚敏. 型壳聚糖凝胶体系的研究及在药剂中的应用[D].上海:上海交通大学,2009.
17. ALVAREZ M M, KHOURY J T, SCHAAFF T G, et al. Optical absorption spectra of nanocrystal Gold molecules[J]. J Phys Chem B, 1997, 101(19): 3706-3712.
18. HUNT P R, KELTNER Z, GAO X, et al. Bioactivity of nanosilver in Caenorhabditis elegans:Effects of size,coat,and shape[J]. Toxicology Reports, 2014, 1: 923-944.
19. SORLIER P, DENUZIERE A, VITON C, et al. Relation between the degree of acetylation and the electrostatic properties of chitin and chitosan[J]. Biomacromolecules, 2001, 2(3): 765-772.
20. JAYAKUMAR R, PRABAHARAN M, SUDHEESH KUMAR P T, et al. Biomaterials based on chitin and chitosan in wound dressing applications[J]. Biotechnol Adv, 2011, 29(3): 322-337.
21. RAI M, YADAV A, GADE A. Silver nanoparticles as a new generation of antimicrobials[J]. Biotechnol Adv, 2008, 27(1): 76-83.
22. FENG Q L, WU J, CHEN G Q, et al. A mechanistic study of the antibacterial effect of silver ions on Escherichia coli and Staphylococcus aureus[J]. J Biomed Mater Res, 2000, 52(4): 662-668.
23. SHARMA V K, YNGARD R A, LIN Y. Silver nanoparticles: Green synthesis and their antimicrobial activities[J]. Adv Colloid Interface Sci, 2009, 145(1/2): 83-96.
24. 韩永萍, 李可意,杨宏伟,等.壳聚糖的抗菌机理及其化学改性研究[J].化学世界,2012,53(4):248-252.

Journal of Biomedical Engineering

Preparation and Characterization of Chitosan-Poloxamer-based Antibacterial Hydrogel Containing Silver Nanoparticles

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