Effects of Different Preservation Methods on Mechanical Properties of Mouse Femur_Journal of Biomedical Engineering

Authors：

CHENGPengzhen ¹ , NINGFenru ² , LIDan ¹ , LIDonglin ¹ , JIANGHuijie ¹ , PEIGuoxian ¹ ,  YANGLiu ¹

1. Xijing Orthopaedic Hospital The Fouth Military Medical University, Xi'an 710032, China;
2. The First Affiliated Hospital Of Xi'an Jiao Tong University, Xi'an 710061, China;

Corresponding author：

YANGLiu, Email: yangliu@fmmu.edu.cn

Keywords：

biomechanics; deflection; elastic modulus; three point bending

DOI：

10.7507/1001-5515.20160180

Video：

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In order to establish the best procedure to store the femur samples from the biomechanical viewpoint, we compared the effects of different storage methods on the mechanical properties of mouse femurs. We obtained femurs surgically from twenty C57BL/6J female mice, 12 weeks old, and randomly divided them into 5 groups, i.e. fresh control group, 4% paraformaldehyde fixation group, 4℃ storage group, -20℃ storage group and -80℃ storage group, respectively, with five mice in each group. For the three low-temperature storage groups, each group was stored for 1 week, 2 months, 6 months at their respective temperatures. After rewarming, three-point bending test was performed to test the load and deflection changes. The results showed that both the elastic modulus and deflection decreased significantly in the 4% paraformaldehyde group. The maximum load and elastic modulus of the samples in the 4℃ group after one week storage was significantly reduced; The mechanical properties were close to the fresh control group in the -20℃ group stored for 2 months but the maximum load was also reduced after 6 months. However, mechanical properties, such as elastic load, maximum load and elastic modulus, were not changed obviously in the -80℃ storage group. Accordingly, -80℃ cryopreservation had little influence on the mechanical properties of bone tissues, which proved that the temperature -80℃ is a suitable one for long-term preservation.

Citation： CHENGPengzhen, NINGFenru, LIDan, LIDonglin, JIANGHuijie, PEIGuoxian, YANGLiu. Effects of Different Preservation Methods on Mechanical Properties of Mouse Femur. Journal of Biomedical Engineering, 2016, 33(6): 1133-1138. doi: 10.7507/1001-5515.20160180 Copy

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1. 史念珂, 富东慧,蔡霞.大鼠股骨生物力学实验研究[J].天津大学学报,2006,39(4):458-462.
2. DAI Ruchun, LIAO Er'yuan, YANG Chuan, et al. Microcracks: an alternative index for evaluating bone biomechanical quality[J]. J Bone Miner Metab, 2004, 22(3): 215-223.
3. 宋亿楠, 李丽辉,张玲莉,等.穴位埋线对老年性骨质疏松小鼠骨密度及骨生物力学的影响[J].中国骨质疏松杂志,2015,21(1):19-24.
4. 马信龙, 马剑雄,徐卫国,等.骨科生物力学研究的测量方法学专家共识[J].中国骨质疏松杂志,2014,20(9):1039-1054.
5. KANG Q, AN Y H, FRIEDMAN R J. Effects of multiple freezing-thawing cycles on ultimate indentation load and stiffness of bovine cancellous bone[J]. Am J Vet Res, 1997, 58(10): 1171-1173.
6. 王法, 黄耀添,殷琦,等.周围神经缺血再灌注损伤的实验研究[J].中华骨科杂志,1996,16(11):723-725.
7. 林坚涛, 吴铁,于琼,等.补中益气汤对环磷酰胺致骨质疏松小鼠骨生物力学的影响[J].中国组织工程研究与临床康复,2007,11(6):1159-1161, 1164.
8. 郭旭, 彭江,王玉,等.腺病毒介导NELL1促进骨溶解后的高质量骨再生[J].中国矫形外科杂志,2012,20(9):834-837.
9. LILL C A, HESSELN J, SCHLEGEL U, et al. Biomechanical evaluation of healing in a non-critical defect in a large animal model of osteoporosis[J]. J Orthop Res, 2003, 21(5): 836-842.
10. 黄泽昊, 侯艳丽,李君平,等.镉对小鼠骨生物力学及成骨细胞MG-63的影响[J].环境化学,2012,31(12):1835-1839.
11. 秦岭, 梁国穗.骨生物力学在防止骨质疏松药物开发中的应用基础(一)[J].中国骨质疏松杂志,2000,6(1):23-25.
12. 刘文和, 雷光华,李康华,等.被动吸烟大鼠骨量与骨生物力学指标的相关性及淫羊藿黄酮的干预效应[J].中国组织工程研究与临床康复,2008,12(20):3905-3909.
13. 张雪竹, 彭应梅,于建春,等.针刺肾俞穴对SAMP6小鼠股骨生物力学性能的影响[J].中国中西医结合杂志,2008,28(6):518-521.
14. 滕宇, 彭海波,彭明波,等.成骨活性肽对骨折愈合生物力学性能的影响[J].骨科,2012,3(2):65-68.
15. 申才良, 刘斌,唐天驷,等.冷冻、冻干、辐照对用于脊柱融合的胫骨皮质骨力学性能的影响[J].医用生物力学,2016,31(1):61-66.
16. 宋永枝, 崔涛,吴成平.不同保存方法对肌腱生物力学特征的影响[J].洛阳医专学报,2002,20(1):16-17.

Journal of Biomedical Engineering

Effects of Different Preservation Methods on Mechanical Properties of Mouse Femur

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