Real-time investigation of dynamic morphology of live platelets and generation of platelet microparticles using hopping probe ion conductance microscopy_Journal of Biomedical Engineering

Authors：

LIU Xiao ¹ , LUO Yufu ² ,  ZHANG Yanjun ³

1. China National Academy of Nanotechnology & Engineering, Tianjin 300457, P.R.China;
2. Tianjin Yongjiu Hospital, Tianjin 300450, P.R.China;
3. Department of Neurosurgery, Tianjin Medical University General Hospital; Tianjin Neurological Institute; Tianjin 300052, P.R.China;

Corresponding author：

ZHANG Yanjun, Email: yanjun_zhang@hotmail.com

Keywords：

hopping probe ion conductance microscopy; platelet activation; platelet microparticles

DOI：

10.7507/1001-5515.201611004

Video：

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Abstract Full text Figures/Tables Video References Cited by

Platelets are rapidly activated by activators and produce a large number of platelet microparticles (PMPs) with high coagulation activity, resulting in coagulation dysfunction. However, the generation mechanism of PMPs is still not clear. Hopping probe ion conductance microscopy (HPICM) has special technical advantages in non-contact, real-time, high-resolution imaging of living cells under physiological conditions. Using HPICM, this study monitored the processes of platelet activation and generation of PMPs in real time in the presence of calcium ionophore A23187 and cytochalasin D (CD), respectively. The results proved that the intracellular calcium concentration and the cytoskeletal proteins played important roles in the platelet activation and the generation of PMPs. Compared with the low density spread shape platelets (LDSS), the high density bubble shape platelets (HDBS) were more sensitive to the calcium ionophore A23187 and cytochalasin D. This research has a guiding significance for the further study on the relationship between platelet activation and coagulation function using HPICM.

Citation： LIU Xiao, LUO Yufu, ZHANG Yanjun. Real-time investigation of dynamic morphology of live platelets and generation of platelet microparticles using hopping probe ion conductance microscopy. Journal of Biomedical Engineering, 2017, 34(5): 767-771. doi: 10.7507/1001-5515.201611004 Copy

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13.	Novak P, Li C, Shevchuk A I, et al. Nanoscale live-cell imaging using hopping probe ion conductance microscopy. Nat Methods, 2009, 6(4): 279-281.
14.	Yang X, Liu X, Lu H, et al. Real-time investigation of acute toxicity of ZnO nanoparticles on human lung epithelia with hopping probe ion conductance microscopy. Chem Res Toxicol, 2012, 25(2): 297-304.
15.	Liu X, Yang X, Zhang B, et al. High-resolution morphological identification and characterization of living neuroblastoma SK-N-SH cells by hopping probe ion conductance microscopy. Brain Res, 2011, 1386: 35-40.
16.	Rheinlaender J, Geisse N A, Proksch R, et al. Comparison of scanning ion conductance microscopy with atomic force microscopy for cell imaging. Langmuir, 2011, 27(2): 697-704.
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19.	杨茜, 刘晓, 马丽颖, 等. 非接触式扫描离子电导显微镜技术在探测活体细胞表面微结构中的应用. 电子显微学报, 2009, 28(5): 489-494.0.

1. Siljander P R. Platelet-derived microparticles-an updated perspective. Thromb Res, 2011, 127(Suppl 2): S30-S33.
2. Morel O, Jesel L, Freyssinet J M, et al. Cellular mechanisms underlying the formation of circulating microparticles. Arterioscler Thromb Vasc Biol, 2011, 31(1): 15-26.
3. Jy W, Horstman L L, Ahn Y S. Microparticle size and its relation to composition, functional activity, and clinical significance. Semin Thromb Hemost, 2010, 36(8): 876-880.
4. Morel N, Morel O, Petit L, et al. Generation of procoagulant microparticles in cerebrospinal fluid and peripheral blood after traumatic brain injury. J Trauma, 2008, 64(3): 698-704.
5. 黄曼, 蔡华波, 胡悦育, 等. 颅脑外伤患者血液中微粒促凝活性的变化. 中华医学杂志, 2009, 89(32): 2265-2268.0.
6. Sinauridze E I, Kireev D A, Popenko N Y, et al. Platelet microparticle membranes have 50- to 100-fold higher specific procoagulant activity than activated platelets. Thromb Haemost, 2007, 97(3): 425-434.
7. Doeuvre L, Plawinski L, Toti F, et al. Cell-derived microparticles: a new challenge in neuroscience. J Neurochem, 2009, 110(2): 457-468.
8. Mobarrez F, Antovic J, Egberg N, et al. A multicolor flow cytometric assay for measurement of platelet-derived microparticles. Thromb Res, 2010, 125(3): e110-e116.
9. Karagkiozaki V, Logothetidis S, Kalfagiannis N, et al. Atomic force microscopy probing platelet activation behavior on titanium nitride nanocoatings for biomedical applications. Nanomedicine, 2009, 5(1): 64-72.
10. Yuana Y, Oosterkamp T H, Bahatyrova S, et al. Atomic force microscopy: a novel approach to the detection of nanosized blood microparticles. J Thromb Haemost, 2010, 8(2): 315-323.
11. 韩立, 韩冬, 王秀凤, 等. 扫描探针显微镜进行细胞扫描时探针对于细胞活性的影响. 电子显微学报, 2003, 22(2): 92-96.0.
12. Liu X, Li Y, Zhu H, et al. Use of non-contact hopping probe ion conductance microscopy to investigate dynamic morphology of live platelets. Platelets, 2015, 26(5): 480-485.
13. Novak P, Li C, Shevchuk A I, et al. Nanoscale live-cell imaging using hopping probe ion conductance microscopy. Nat Methods, 2009, 6(4): 279-281.
14. Yang X, Liu X, Lu H, et al. Real-time investigation of acute toxicity of ZnO nanoparticles on human lung epithelia with hopping probe ion conductance microscopy. Chem Res Toxicol, 2012, 25(2): 297-304.
15. Liu X, Yang X, Zhang B, et al. High-resolution morphological identification and characterization of living neuroblastoma SK-N-SH cells by hopping probe ion conductance microscopy. Brain Res, 2011, 1386: 35-40.
16. Rheinlaender J, Geisse N A, Proksch R, et al. Comparison of scanning ion conductance microscopy with atomic force microscopy for cell imaging. Langmuir, 2011, 27(2): 697-704.
17. Zhang Y, Liu X, Liu L, et al. Contact- and agonist-regulated microvesiculation of human platelets. Thromb Haemost, 2013, 110(2): 331-339.
18. 刘晓, 杨茜, 张晓帆, 等. 利用探针跳跃式扫描离子电导显微镜研究活体神经细胞拓扑结构. 生物医学工程学报, 2010, 27(6): 1365-1369.0.
19. 杨茜, 刘晓, 马丽颖, 等. 非接触式扫描离子电导显微镜技术在探测活体细胞表面微结构中的应用. 电子显微学报, 2009, 28(5): 489-494.0.

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