High throughput detection and characterization of red blood cells deformability by combining optical tweezers with microfluidic technique_Journal of Biomedical Engineering

Authors：

ZHANG Meng ,  MENG Xiaochen , ZHU Lianqing

Beijing Key Laboratory for Optoelectronic Measurement Technology, Beijing Laboratory of Biomedical Testing Technology and Instruments, Beijing Information Science and Technology University, Beijing 100101, P.R.China;

Corresponding author：

MENG Xiaochen, Email: mengxc@bistu.edu.cn

Keywords：

red blood cells deformability; microfluidic chip; optical tweezer; high throughput detection

DOI：

10.7507/1001-5515.201911020

Video：

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A high throughput measurement method of human red blood cells (RBCs) deformability combined with optical tweezers technology and the microfluidic chip was proposed to accurately characterize the deformability of RBCs statistically. Firstly, the effective stretching deformation of RBCs was realized by the interaction of photo-trapping force and fluid viscous resistance. Secondly, the characteristic parameters before and after the deformation of the single cell were extracted through the image processing method to obtain the deformation index of area and circumference. Finally, statistical analysis was performed, and the average deformation index parameters (, ) were used to characterize the deformability of RBCs. A high-throughput detection system was built, and the optimal experimental conditions were obtained through a large number of experiments. Three groups of samples with different deformability were used for statistical verification. The results showed that the smallest cell component was 9.71%, and the detection flux of 8-channel structure was about 370 cells/min. High-throughput detection and characterization methods can effectively distinguish different deformed RBCs statistically, which provides a solution for high-throughput deformation analysis of other types of samples.

Citation： ZHANG Meng, MENG Xiaochen, ZHU Lianqing. High throughput detection and characterization of red blood cells deformability by combining optical tweezers with microfluidic technique. Journal of Biomedical Engineering, 2020, 37(5): 848-854. doi: 10.7507/1001-5515.201911020 Copy

1.	向甄, 杨继庆, 文峻. 红细胞变形性的影响因素及测量方法. 中国医学物理学杂志, 2006, 23(6): 423-426.
2.	张燕, 梅同华, 吴泽志. 疏血通对慢性肺心病急性加重患者红细胞膜黏弹特性的影响. 生物医学工程学杂志, 2012, 29(1): 134-136.
3.	龚琬玲, 黄时俊, 司歌, 等. 多模式音频脉冲信号光电穴位刺激对高血压大鼠血液流变性及血压的影响. 生物医学工程学杂志, 2012, 29(3): 415-419.
4.	李一凡. 光镊的发展——追寻光的力量. 内燃机与配件, 2018(6): 250-251.
5.	李银妹, 龚雷, 李迪, 等. 光镊技术的研究现况. 中国激光, 2015, 42(1): 9-28.
6.	Song H, Liu Y, Zhang B, et al. Study of in vitro RBCs membrane elasticity with AOD scanning optical tweezers. Biomed Opt Expr, 2017, 8(1): 384-394.
7.	Zhong M C, Wei X B, Zhou J H, et al. Trapping red blood cells in living animals using optical tweezers. Nat Commun, 2013, 4: 1768.
8.	孙东, 罗涛. 基于微流控的细胞操纵方法与应用. 科技导报, 2018, 36(16): 29-38.
9.	侯建勋, 申世铉. 基于微流控和机器视觉的红细胞变形性测量系统. 中国医疗器志, 2016, 40(3): 173-175.
10.	吴春卉, 姜有为, 程鑫. 微流控芯片在单细胞捕获中的应用. 科技导报, 2018, 36(16): 39-45.
11.	Stauber H, Waisman D, Korin N, et al. Red blood cell (RBC) suspensions in confined microflows: Pressure-flow relationship. Med Eng Phys, 2017, 48: 49-54.
12.	李成宇, 张玉灵, 周哲海. 基于 Hough 变换处理方法的光镊光阱刚度标定. 激光与红外, 2017, 47(10): 1210-1215.
13.	Lee K, Muravyov A, Semenov A, et al. Optical tweezers for measuring the interaction of the two single red blood cells in flow condition// Saratov Fall Meeting 2016: Optical Technologies in Biophysics and Medicine XVIII. Saratov: International Society for Optics and Photonics, 2017, 10336: 1033605.
14.	Tomaiuolo G, Lanotte L, D’Apolito R, et al. Microconfined flow behavior of red blood cells. Med Eng Phys, 2015, 38(1): 11-16.
15.	刘畅. 基于 PDMS 的红细胞变形性微通道芯片研究. 重庆: 重庆大学, 2010.
16.	Smart T J, Richards C J, Bhatnagar R, et al. A study of red blood cell deformability in diabetic retinopathy using optical tweezers// Optical Trapping and Optical Micromanipulation XII. San Diego: International Society for Optics and Photonics, 2015, 9548: 954825.
17.	宋华东. 基于光镊技术的离体红细胞弹性研究. 徐州: 江苏师范大学, 2017.
18.	周新丽, 衣星越, 周楠峰, 等. 用于低温保护剂混合的微流控芯片设计及优化. 生物医学工程学杂志, 2016, 33(3): 461-465.
19.	衣星越, 周新丽, 杨云, 等. 微流控法去除低温保护剂对卵母细胞发育的影响. 生物医学工程学杂志, 2018, 35(1): 123-130.
20.	郑小林, 吴楠, 卢晓, 等. 一种新的硅微通道红细胞变形性测量方法. 中国生物医学工程学报, 2000, 19(1): 7-12.
21.	韩威俊. 微通道内红细胞运动与受力的实验研究//中国力学大会-2017暨庆祝中国力学学会成立60周年大会论文集. 北京: 中国力学学会, 2017: 9.
22.	刘振文. PDMS微流控芯片注塑成型研究. 北京: 北京化工大学, 2019.
23.	任春艳, 马圆圆, 王景冉, 等. 微流控芯片设计和应用. 实验技术与管理, 2018, 35(10): 84-87.
24.	Liu Jiaqi, Zhu Lianqing, Zhang Fan, et al. Microdeformation of RBCs under oxidative stress measured by digital holographic microscopy and optical tweezers. Appl Opt, 2019, 58(15): 4042.
25.	Liu Jiaqi, Zhang Fan, Zhu Lianqing, et al. Mechanical properties of RBCs under oxidative stress measured by optical tweezers. Opt Commun, 2019, 442: 56-59.

1. 向甄, 杨继庆, 文峻. 红细胞变形性的影响因素及测量方法. 中国医学物理学杂志, 2006, 23(6): 423-426.
2. 张燕, 梅同华, 吴泽志. 疏血通对慢性肺心病急性加重患者红细胞膜黏弹特性的影响. 生物医学工程学杂志, 2012, 29(1): 134-136.
3. 龚琬玲, 黄时俊, 司歌, 等. 多模式音频脉冲信号光电穴位刺激对高血压大鼠血液流变性及血压的影响. 生物医学工程学杂志, 2012, 29(3): 415-419.
4. 李一凡. 光镊的发展——追寻光的力量. 内燃机与配件, 2018(6): 250-251.
5. 李银妹, 龚雷, 李迪, 等. 光镊技术的研究现况. 中国激光, 2015, 42(1): 9-28.
6. Song H, Liu Y, Zhang B, et al. Study of in vitro RBCs membrane elasticity with AOD scanning optical tweezers. Biomed Opt Expr, 2017, 8(1): 384-394.
7. Zhong M C, Wei X B, Zhou J H, et al. Trapping red blood cells in living animals using optical tweezers. Nat Commun, 2013, 4: 1768.
8. 孙东, 罗涛. 基于微流控的细胞操纵方法与应用. 科技导报, 2018, 36(16): 29-38.
9. 侯建勋, 申世铉. 基于微流控和机器视觉的红细胞变形性测量系统. 中国医疗器志, 2016, 40(3): 173-175.
10. 吴春卉, 姜有为, 程鑫. 微流控芯片在单细胞捕获中的应用. 科技导报, 2018, 36(16): 39-45.
11. Stauber H, Waisman D, Korin N, et al. Red blood cell (RBC) suspensions in confined microflows: Pressure-flow relationship. Med Eng Phys, 2017, 48: 49-54.
12. 李成宇, 张玉灵, 周哲海. 基于 Hough 变换处理方法的光镊光阱刚度标定. 激光与红外, 2017, 47(10): 1210-1215.
13. Lee K, Muravyov A, Semenov A, et al. Optical tweezers for measuring the interaction of the two single red blood cells in flow condition// Saratov Fall Meeting 2016: Optical Technologies in Biophysics and Medicine XVIII. Saratov: International Society for Optics and Photonics, 2017, 10336: 1033605.
14. Tomaiuolo G, Lanotte L, D’Apolito R, et al. Microconfined flow behavior of red blood cells. Med Eng Phys, 2015, 38(1): 11-16.
15. 刘畅. 基于 PDMS 的红细胞变形性微通道芯片研究. 重庆: 重庆大学, 2010.
16. Smart T J, Richards C J, Bhatnagar R, et al. A study of red blood cell deformability in diabetic retinopathy using optical tweezers// Optical Trapping and Optical Micromanipulation XII. San Diego: International Society for Optics and Photonics, 2015, 9548: 954825.
17. 宋华东. 基于光镊技术的离体红细胞弹性研究. 徐州: 江苏师范大学, 2017.
18. 周新丽, 衣星越, 周楠峰, 等. 用于低温保护剂混合的微流控芯片设计及优化. 生物医学工程学杂志, 2016, 33(3): 461-465.
19. 衣星越, 周新丽, 杨云, 等. 微流控法去除低温保护剂对卵母细胞发育的影响. 生物医学工程学杂志, 2018, 35(1): 123-130.
20. 郑小林, 吴楠, 卢晓, 等. 一种新的硅微通道红细胞变形性测量方法. 中国生物医学工程学报, 2000, 19(1): 7-12.
21. 韩威俊. 微通道内红细胞运动与受力的实验研究//中国力学大会-2017暨庆祝中国力学学会成立60周年大会论文集. 北京: 中国力学学会, 2017: 9.
22. 刘振文. PDMS微流控芯片注塑成型研究. 北京: 北京化工大学, 2019.
23. 任春艳, 马圆圆, 王景冉, 等. 微流控芯片设计和应用. 实验技术与管理, 2018, 35(10): 84-87.
24. Liu Jiaqi, Zhu Lianqing, Zhang Fan, et al. Microdeformation of RBCs under oxidative stress measured by digital holographic microscopy and optical tweezers. Appl Opt, 2019, 58(15): 4042.
25. Liu Jiaqi, Zhang Fan, Zhu Lianqing, et al. Mechanical properties of RBCs under oxidative stress measured by optical tweezers. Opt Commun, 2019, 442: 56-59.

Journal of Biomedical Engineering

High throughput detection and characterization of red blood cells deformability by combining optical tweezers with microfluidic technique

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